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Chitooligosaccharides attenuate UVB-induced damages in human dermal fibroblasts.
Ahn, Byul-Nim,Kim, Jung-Ae,Himaya, S W A,Bak, Soon-Sun,Kong, Chang-Suk,Kim, Se-Kwon Springer-Verlag 2012 Naunyn-Schmiedeberg's archives of pharmacology Vol.385 No.1
<P>As a continuation of our research on chitooligosaccharides (COS), this study focused on the protective effect of COS of various molecular weights (1-3, 3-5, and 5-10?kDa) on cellular damage caused by ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblast cells. The results show that the protective effect of COS on UVB-stressed human fibroblasts was dependent on molecular weight. COS suppressed UVB irradiation-induced reactive oxygen species generation and DNA damage, accompanied by the downregulation of matrix metalloproteinase (MMP)-1 and MMP-13. In a comparative analysis, COS (3-5?kDa) exhibited the most potent protective effect on UVB-stressed fibroblasts. The presence of COS (3-5?kDa) attenuated UVB-induced collagenolytic MMP production and collagen degradation. The photoprotective activity of COS (3-5?kDa) was confirmed by transcriptional phosphorylation of mitogen-activated protein kinase-responsive signaling pathways.</P>
복강내 NF-κB decoy oligodeoxynucleotide를 이용한 수술 후 복강내 유착 발생 감소
안별님 ( Byul Nim Ahn,),송미현 ( Mi Hyun Song,),김주현 ( Ju Hyun Kim,),김경현 ( Kyung Hyun Kim ),박관규 ( Kwan Kyu Park ),최윤석 ( Youn Seok Choi ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.4
목적 쥐모델에서 nuclear factor κB decoy oligodeoxynucleotide (NF-κB decoy ODN) 복강내 주입을 이용한 수술 후 복강내 유착 발생 억제 효과를 알아보고자 하였다. 연구방법 40마리의 BALB/c 암컷 생쥐를 ketamine과 xylazine 복강내 주사를 이용하여 마취 후 개복하고 cytobrush를 이용하여 복막이 벗겨지는 상처를 입히고, 실험군은(n = 20) NF-κB decoy ODN (10 μg, 500 μL)을 복강내에 채우고, 대조군은(n=20) 생리 식염수 500 μL를 복강내에 채운 후 봉합하고 2주 뒤에 유착 정도를 판정하였다. 유착 정도는 유착이 없는 0등급에서 심한 정도에 따라 경증, 중등도, 중증 등급(1-3등급)으로 분류하였다. 결과 수술 후 첫날 실험군에서 2마리, 대조군에서 3마리의 생쥐가 사망하였다. 대조군은 경증과 중등도의 유착이 각각 2마리에서 관찰되었고 13마리에서 중증의 유착이 관찰되었으나, 실험군에서 4마리는 유착이 관찰되지 않았고, 6마리는 경증의 유착, 8마리는 중등도의 유착이 관찰되어 통계적으로 유의한 차이를 나타내었다(P<0.001). 결론 NF-κB decoy ODN를 복강내에 주입하여 NF-κB 작용을 차단하는 방법은 수술 후 복강내 유착 정도를 줄일 수 있다. Objective The objective of this study was to evaluate the effect of intra-peritoneal nuclear factor κB (NF-κB) decoy oligodeoxynucleotide on the prevention of postoperative intra-abdominal adhesion. Methods Forty female BALB/c mice were randomly assigned into 2 groups. The mice were anesthetized and then midline abdominal incision was made. Peritoneal injury was given using cytobrush until deperitonealization. The experimental group (n = 20) received NF-κB decoy oligodeoxynucleotide (NF-κB decoy ODN) in the peritoneal cavity before abdominal closure, while the control group (n = 20) received saline. At 2 weeks postoperation, after the mice were euthanized, the status of intra-abdominal adhesion was evaluated. The degrees of adhesion were graded into no (grade 0), mild (grade 1), moderate (grade 2), and severe adhesion (grade 3). Results Two mice in the experimental group and 3 mice in the control group were dead within 1 day postoperation. In the control group, 2, 2, and 13 mice showed mild, moderate, and severe adhesion, respectively. In the experimental group, 4 mice showed no adhesion, whereas, 6 and 8 mice showed mild and moderate adhesion, respectively. The difference of degrees of adhesion between the groups was statistically signifi cant ( P<0.001). Conclusion Intra-peritoneal NF-κB decoy ODN reduced the severity of postoperative intra-abdominal adhesion.