Triptolide suppresses interleukin-1beta-induced human beta-defensin-2 mRNA expression through inhibition of transcriptional activation of NF-kappaB in A549 cells.

Jang, Byeong-Churl,Lim, Ki-Jo,Choi, In-Hak,Suh, Min-Ho,Park, Jong-Gu,Mun, Kyo-Chul,Bae, Jae-Hoon,Shin, Dong-Hoon,Suh, Seong-Il D.A. Spandidos 2007 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.19 No.5

<P>The immunosuppressive effect of triptolide has been associated with suppression of T-cell activation. However, the immunosuppressive effects of triptolide on innate immunity in the epithelial barrier remain to be elucidated. Human beta-defensin (HBD)-2 is an inducible antimicrobial peptide and plays an important role in the innate immunity. We have previously demonstrated that IL-1beta induced HBD-2 mRNA expression in A549 cells through activation of nuclear factor-kappaB (NF-kappaB) transcriptional factor as well as p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK), or phosphatidylinositol-3-kinase (PI3K). In this study, we investigated effects of triptolide on IL-1beta-induced HBD-2 mRNA expression in A549 cells. Triptolide inhibited IL-1beta-induced HBD-2 mRNA expression in a dose-dependent manner. Addition of triptolide did not suppress activation of p38 MAPK, JNK, or PI3K in response to IL-1beta. Triptolide inhibited IL-1beta-induced MAPK phosphatase-1 expression at the transcriptional level and resulted in sustained phosphorylation of JNK or p38 MAPK, explaining the little effect of triptolide on IL-1beta-induced phosphorylation of these kinases. Although triptolide partially suppressed IL-1beta-mediated degradation of IkappaB-alpha and nuclear translocation of p65 NF-kappaB, triptolide potently inhibited NF-kappaB promoter-driven luciferase activity in A549 cells. These results collectively suggest that the inhibitory effect of triptolide on IL-1beta-induced HBD-2 mRNA expression in A549 cells seems to be at least in part mediated through nuclear inhibition of NF-kappaB transcriptional activity, but not inhibition of p38 MAPK, JNK, or PI3K. This inhibition may explain the ability of triptolide to diminish innate immune response.</P>

폐포 대식세포 및 단핵구가 Interleukin-2 Enhanced Natural Killer 및 LAK Activity에 미치는 영향

조철호 ( Jo Cheol Ho ),김병일 ( Kim Byeong Il ),김세규 ( Kim Se Gyu ),천선희 ( Cheon Seon Hui ),김형중 ( Kim Hyeong Jung ),장준 ( Jang Jun ),안철민 ( An Cheol Min ),김성규 ( Kim Seong Gyu ),이원영 ( Lee Won Yeong ),윤정구 ( Yun J 대한내과학회 1992 대한내과학회지 Vol.42 No.5

Alveolar macrophages (AM) are thought to function as primary effector cells against tumors growing in the lung. Systemic administration of lymphokine activated killer (LAK) cells and IL-2 resulted in partial antitumor response in patients with advanced cancer. LAK activity is influenced by various factors. We studied the effects of AM and blood monocytes from healthy donors on IL-2 enhanced NK activity against K-562 cells and LAK activity against Raji cells utilizing a 4h ^(51)Cr release assay. The following results were obtained: 1) The addition of different doses of human blood monocytes showed no suppression or enhancement of IL-2 enhanced NK and LAK activity. 2) The addition of high dose of AM (Lymphocyte: AM=1:1) significantly suppressed IL-2 enhanced NK activity. Smaller doses of AM (Lymphocyte: AM= 10:1and 100:1) did not suppress IL-2 enhanced NK activity. 3) The addition of high dose of AM (Lymphocyte: AM = 1:1 and 10:1) significantly suppressed LAK activity. The smallest dose of AM (Lymphocyte: AM= 100:1) did not suppress LAK activity. In conclusion, IL-2 enhanced NK and LAK activity were dose-dependently suppressed by human alveolar macrophages. However IL-2 enhanced NK and LAK activity were not suppressed by blood monocytes.

기-액병류상향 고정층반응기에서 액체의 흐름과 축방향분산

함병호,조지훈,편무실,김태옥,손민일 明知大學校 産業技術硏究所 1996 産業技術硏究所論文集 Vol.15 No.-

For upward cocurrent gas-liquid flow in a fixed bed reactor, the flow and axial dispersion of liquid were investigated in an air-water-Zeolite particle system. Based on experimental residence time distributions, parameters of mixing models were evaluated with two proposed liquid mixing models: axial dispersion model with stagnant zone(DSM) and plug flow model with stagnant zone(PFM). Accordingly, the applicability and parameters of each mixing model were analyzed for various operating conditions. We found that the flow and axial mixing characteristics of liquid could be analyzed well by DSM and parameters were well estimated by curve fitting using trial and error method. Also, the fraction of stagnant zone was decreased with increasing liquid velocity, while the dimensionless mass transfer coefficient between dynamic and stagnant zones was increased, and these parameters estimated by DSM were lower than those by PFM. In dynamic zone, calculated Peclet number were increased with increasing liquid velocity and axial mixing characteristics of liquid tended to access to plug flow for the high liquid Reynolds number(about Re1>50). For the porous catalyst particle system the flow and axial mixing characteristics of liquid had nearly same tendency that for the nonporous particle(glass bead) system, while dimensionless mass transfer coefficient was lower value than that for it. In these conditions, correlations of these parameters were obtained for various operating conditions.

함암요법으로 골수기능이 억제된 악성종양환자에서 rhG-CSF의 임상적 효과

김원민,서영환,조경상,유병전,김상도,이승일,정춘해 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

We have studied the efficacy of rhG-CSF in patients with non-hodgkin's lymphoma, acute leukemia, and small cell lung cancer undergoing anticancer chemotherapy. These patients were below leukocyte count 3,000 cubic millimeter due to myelosuppression induced by the first cycle of intensive chemotherapy. Treatment with rhG-CSF (100㎍ per square meter of body surface area per day in a 30-minute intravenous infusion) was begun two days and for 14 consecutive days after the end of the second cycle of chemotherapy. The results were as follows. 1. The onset of myelosuppression was 6 days after chemotherapy, and the onset of recovery was 16.7 days after chemotherapy, and the duration of granulocytopenia was 10.7 days in patients with malignant tumor during contrast period. 2. The duration of granulocytopenia was shortened 5.2 days in patients administered rhG-CSF than without rhG-CSF, and we observed the shortest duration of granulocytopenia with increasing granulocyte in patients with small cell lung cancer. 3. Observing the differential count of leukocyte checked the highest level of leukocyte in contrast and rhG-CSF period, the persentage of neutrophil was increased in patients with acute leukemia and small cell lung cancer during rhG-CSF period, but not increased in patients with non-hodgkin's lymphoma. 4. rhG-CSF was not influenced on liver faction, renal fuction, uric acid, and glucose metabolism, also had no effect on recovery of platelet. In conclusion, rhG-CSF can be administered to patients with hematologic or nonhematologic malignant tumor that myelosuppression induced by anticancer chemotherapy result in shortening the duration of granulocytopenia and increasing the peripheral neutrophil, therefore full dose chemotherapy can be administered on time, and rhG-CSF may reduce the morbidity and mortality of patients with malignant tumor undergoing chemotherapy.

다공성 충전층반응기에서 기-액흐름양상의 전이와 액체체류량

손민일,함병호,조지훈,김태옥,장영준 明知大學校 産業技術硏究所 1996 産業技術硏究所論文集 Vol.15 No.-

For both downward and ward cement gas-liquid flow in a porous packed bed reactor the transition of gas-liquid flow pattern and liquid holdup were investigated experimentally in an air-water-Zeolite particle system. Experimental results showed that gas velocity at trickle-to-pulse flow transition boundary in the downflow system was decreased with increasing liquid velocity and that gas velocity at bubble-to-pulse flow transition boundary in the upflow system was nearly constant for lower liquid velocity, however, it was increased with increasing liquid velocity for turbulent flow region. We also found that the dynamic liquid holdup in the upflow system was higher than that in the downflow system and it was increased with increasing liquid velocity and with decreasing gas velocity. Furthermore,the dynamic liquid holdup was sensitively affected by liquid velocity at trickle flow in the downflow system while it was sensitively affected by gas velocity at bubble flow in the upflow system and at pulse flow in the downflow and upflow systems. For the porous particle system fluid velocities at gas-liquid flow transition boundaries and dynamic liquid holdup had nearly same that for the nonporous particle(glass bead) system.

기-액병류상향 충전탑에서 액체체류량과 축방향혼합특성

조지훈,손민일,박용성,함병호,김태옥 明知大學校 産業技術硏究所 1993 産業技術硏究所論文集 Vol.12 No.-

For upward cocurrent gas-liquid flow in a packed column, holdup and axial mixing characteristics of liquid were investigated. In an air-water-glass bead system. liquid holdup and residence time distribution(RTD) were measured and Peclet number based on axial dispersion model was evaluated from experimental RTD. We found that liquid holdup was increased with increasing liquid velocity and size of packing material, and it was decreased with increasing gas velocity. Also, liquid holdup for the cocurrent up flow system was larger than that for the downflow system at the same experimental conditions. As increasing gas velocity, the in fluence of packing size and operation method on liquid holdup was reduced. The calculated Peclet number was not affected by gas velocity, and it was increased with increasing liquid velocity and with decreasing packing size. Furthermore, the effect of liquid velocity on Petlet number for higher liquid Reynolds number(Re1>70) was larger than that for lower Re1. In these conditions, correlations of the dynamic liquid holdup and the Bodenstein number were obtained.

자발성 뇌지주막하출혈 환자에서 Cardiac Troponin Ⅰ를 이용한 심근손상의 발생율

김용권,류진호,소정일,문원식,전병조,허탁,민용일 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : More than 90% of acute stroke patients have measurable cardiovascular sequelae, but we have been often overlooked in formal discussions of treatment. If we estimate the incidence of myocardial injury in patients with spontaneous SAH, we may figure the possibility of cardiac dysfunction in such patients. This study was designed to investigate the incidence of myocardial injury in patients with spontaneous SAH using cardiac troponin I(cTnI). Methods : A prospective single emergency center study was performed to determined preoperative incidence of unrecognized cardiac injury in patients suffering spontaneous SAH. We include the spontaneous SAH patients who underwent serum measurements of the cardic troponin I immediately upon admission last six month period. ECG, CK, CK-MB and myoglobin were also performed at admission. We excluded the spontaneous SAH patients who had past history of myocardial ischemia and ECG abnormality. Results : Fifty-two patients(34 females, 18 males) with spontaneous SAH were studied prospectively. 18 patients(34.6% of the total study population) had cTnI level above 0.5ng/ml. ECG was performed in 52 patients and was abnormal in 15 of the 52 patients(28.8%). Conclusion : The measurement of cTnI has provided physicians with a myocardial marker that has a cardiac sensitivity for cardiac injury equal to that of CK-MB yet with greater specificity. So, cardiac troponin I is useful to estimate the incidence of myocardial injury in patients with spontaneous SAH. And we may estimate the possibility of cardiac dysfunction in such patients. This knowledge will hopefully aid in the care and improve the outcome.

전기적화상에 의한 구순결손의 재건 -증례보고-

박진규,김경원,김병린,민병일 大韓顎顔面成形再建外科學會 1990 Maxillofacial Plastic Reconstructive Surgery Vol.12 No.3

Electrical burns of the lips are most frequently seen in small children, who are apt to chew on electrical cord or plug, the ends of extension cords in their mouth, saliva creates a short circuit across the terminals within the plug, causing an electrical burn. Tissue destruction with electrical burns is sudden and extensive. Extensive, deep coagulation necrosis is instaneously produced by the extreme temparatures of electrical arc. If the child is well grounded, the circuit flow through his body may cause cardiac arrest. The purpose of this report is to document two cases of electrical lip burn and reconstruction of the lip defect with some local flap techniques. For case 1, Z plasty & V - Y plasty and lengthening of the commissure and in case 2, Abbe flap technique was used and scar was revised later. Z - plasty and V- Y plastry were used for scar release and Abbe flap was designed on lower lip to meet the need of upper lip. For short of right lip width, lengthening of the commissure was done. We are to report the improvement with forementioned operation on the patient of electrical burn upon the lip.

Effects of Operating Condition on the 2-dimensional Temperature and Velocity Distributions of a Thermal Plasma Jet in a Segmented Arc Heater

Byeong-Il Min,Juyoung Ko,Sooseok Choi,Tae-Hee Kim 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.5

A segmented arc heater with an input power of 2.5 MW for thermal protection studies of a re-entry vehicle was numerically simulated to determine its appropriate operating conditions. A self-developed magnetohydrodynamics code was used to analyze the 2-dimensional characteristics of the thermal plasma jet in the segmented arc heater. Operating ranges of the air ow rate and the arc current were from 20 to 60 g/s and from 400 to 800 A, respectively. Although the velocity of the thermal plasma jet and the input power were increased by increasing the air ow rate, the plasma enthalpy, which is proportional to the temperature, was decreased at a high air ow rate because of the cooling effect. On the other hand, an increase in the arc current had the positive effect of increasing the plasma temperature. As a result, the target enthalpy of over than 20 MJ/kg was achieved for an air ow rate of 40 g/s and an arc current above 600 A.

악하선에 발생한 Küttner 종양 1예

최병일(Byeong Il Choi),강민석(Min Seok Kang),김민기(Min Ki Kim),임범진(Beom Jin Lim),변형권(Hyung Kwon Byeon) 대한두경부종양학회 2015 대한두경부 종양학회지 Vol.31 No.2

Küttner tumor is a benign inflammatory disease of the salivary gland that clinically mimics a malignant tumor because of its presentation as a unilateral painless hard mass. However the precise cause of this disease is still unknown. Pathologically, it is characterized by infiltration of IgG4-positive plasma cells. Also, it is accompanied with an elevated level of plasma IgG4. We report one case of Küttner tumor of the right submandibular gland in a 37 year old woman.