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- 저자 : Berenice Hernandez (검색결과 3 건)
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( Dionicio Fuentes-aragon ),( Sandra Berenice Juarez-vazquez ),( Mateo Vargas-hernandez ),( Hilda Victoria Silva-rojas ) 한국균학회 2018 Mycobiology Vol.46 No.2
The filamentous Ascomycota Colletotrichum gloeosporioides sensu lato is a fungus that has been reported worldwide as a causal agent of anthracnose disease in avocado and other crops. In Mexico, this species affects fruits from an early stage of development in the orchard until the post-harvest stage. Although fungicides are continuously applied to control Colletotrichum species, pericarp cankers and soft rot mesocarp in fruits are still frequently observed. Considering the lack of a precise description of the causative agent, the aim of the current study was to determine the pathogens involved in this symptomatology. Twenty-four isolates were consistently obtained from the pericarp of avocado fruits cv. “Hass” collected in the central avocado-producing area of Mexico. Morphological features such as colony growth, conidia size, and mycelial appressorium were assessed. Bayesian multilocus phylogenetic analyses were performed using amplified sequences of the internal transcribed spacer region of the nuclear ribosomal DNA; actin, chitin synthase, glyceraldehyde-3-phosphate dehydrogenase partial genes; and APn2-Mat1-2 intergenic spacer and mating type Mat1-2 partial gene from the nine selected isolates. In addition, fruits were inoculated with a conidial suspension and reproducible symptoms confirmed the presence of Colletotrichum fructicola in this area. This pathogenic species can now be added to those previously reported in the country, such as C. acutatum, C. boninense, C. godetiae, C. gloeosporioides, and C. karstii. Disease management programs to reduce the incidence of anthracnose should include C. fructicola to determine its response to fungicides that are routinely applied, considering that the appearance of new species is affecting the commercial quality of the fruits and shifting the original population structure.
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Electrochemical oxidation of methyl orange azo dye at pilot flow plant using BDD technology
Cecilia Ramırez,Juan M. Peralta-Hernandez,Adriana Saldana,Berenice Hernandez,Roberto Acero,Ricardo Guerra,Sergi Garcia-Segura,Enric Brillas 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.2
Solutions of methyl orange azo dye were degraded by electrochemical oxidation using a 3 L flow plant with a boron-doped diamond (BDD)/stainless steel cell operating at constant current density, ambient temperature and liquid flow rate of 12 L min-1. A 23 factorial design considering the applied current density, azo dye concentration and electrolysis time as variable independents was used to analyze the process by response surface methodology. LC–MS analysis revealed the formation of seven oxidation products from the cleavage of the –N55N– group of the dye, followed by deamination, formation of a nitro group and/or desulfonation of the resulting aromatics.
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Mariela Jimenez,Fabiola C. Munoz,Daniel Cervantes-Garcıa,Maritza M. Cervantes,Alicia Hernandez-Mercado,Berenice Barron-Garcıa,Jose L. Moreno Hernandez-Duque,Adrian Rodrıguez-Carlos,Bruno Rivas-Santiag 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.11
The maintenance of a healthy skin barrier is crucial to prevent and treat atopic dermatitis (AD) lesions and avoid infections. Glycomacropeptide (GMP) is a bioactive peptide that has demonstrated promising results as an anti-inflammatory and antipruritic therapy for experimental AD. This study aimed to analyze the effect of GMP on impaired cutaneous barrier-related signs in a rat model of AD lesions. AD-like dermatitis was induced on the skin by repeated topical applications of 2,4-dinitrochlorobenzene, and animals were orally administered GMP before or after AD induction. The expression of skin structural proteins and antimicrobial peptides (AMPs) was evaluated by immunoblot or immunohistochemistry, epidermal thickening was evaluated by histochemistry, the level of IFN-γ and changes in the microbiota were evaluated by quantitative polymerase chain reaction, and the quantity of fecal short-chain fatty acids (SCFAs) was evaluated by gas chromatography. GMP administration significantly increased filaggrin, β-defensin 2, and cathelicidin-related AMP expression in AD-like lesions. Involucrin expression was not modified. In GMP-treated animals, epidermal thickening and IFN-γ expression were strongly reduced in damaged skin. GMP treatment impacted the skin microbiota and prevented Staphylococcus aureus colonization, which is associated with AD. In addition, high levels of Bifidobacterium were detected in the feces of GMP-treated animals, and the acetic acid and butyric acid contents increased in animals prophylactically administered GMP. These results suggest that GMP markedly prevents or reverses skin barrier damage in rat AD-like lesions through a bifidogenic effect that induces fecal SCFA production with prolonged treatment. Our findings provide evidence that GMP may represent an optimum strategy for the therapy of the dysfunctional cutaneous barrier in AD.
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