서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

Lee, Eun-Mi,Lee, Young-Eun,Lee, Esder,Ryu, Gyeong Ryul,Ko, Seung-Hyun,Moon, Sung-Dae,Song, Ki-Ho,Ahn, Yu-Bae Korean Diabetes Association 2011 Diabetes and Metabolism Journal Vol.35 No.5

<P><B>Background</B></P><P>Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis.</P><P><B>Methods</B></P><P>Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.</P><P><B>Results</B></P><P>CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.</P><P><B>Conclusion</B></P><P>Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.</P>

소양호에서 세균수와 세균 체적의 계절적 수심별 변화

안태석,최승익,변명섭,박호완 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

To define the ecological roles of bacteria in Lake Soynng, the depth profiles of bacterial numbers and their biovolume were estimated temporally. In August, 1992, the bacterial numbers and biovolume were ranged from 12×10^(5) to 28×10^(5) cells ml^(-1) and from 0.4 to 1.0 ㎛³ cell^(-1), respectively. At 30m depth, the biovolume was highest as 1.0㎛³ cell^(-1) while the numbers were not changed. These large bacteria might be allochthonous bacteria originated from terrestrial during the heavy rain. In October, 1882, the bacterial numbers were ranged from 6×10^(5) to 17×10^(5) cells ml³ , and biovolumes at epilimnion were about 0.5 ㎛³ cell^(-1), while at metalimnion and hypolimnion, larger than 0.9 ㎛³ cell^(-1). In this time, zooplankton grazing pressure and nutrients release from sediment might affect the bacterial numbers and biovolume. In April, 1993, when Asterionella and Fragifaria were dominant species of phytoplankton only in epilimnion, the bacterial numbers were about 20×10^(5) cells ml^(-1), and biovolumes were about 0.8㎛³ cell^(-1). While at other depths, the numbers and biovolumes were smaller. These results suggest that the bacterial numbers were dynamically changed with season, while their mean cell volume were commonly stable as 0.5㎛³ cell^(-1). But by the allochthonous bacteria, organic materials released by phytoplnnkton and grazing pressure by zooplankton might increase the biovolume.

뇌졸중 환자의 경직 감소를 위한 경피적 전기 신경 자극술과 침술의 효과

백천호,소성욱,김희상,안경회,남상수,박선구 大韓再活醫學會 1997 Annals of Rehabilitation Medicine Vol.21 No.6

세계철도의 고속화와 재래철도 속도 향상에 대한 연구

安勝虎 철도전문대학교 1996 論文集 Vol.12 No.1

최대 오차 벡터를 이용한 최적 공간 벡터 변조 기법에 관한 연구

안호균,김태엽,진승오,남징락 國立 昌原大學校 産業技術硏究所 1998 産技硏論文集 Vol.12 No.-

This study proposes a optimized space vector modulation(SVM), which makes a good output current with low total harmonic distortion(THD) and introduces the concept of maximum error vector for new method. The performance of the new modulation technique using maximum error vector is shown by simulation and experiments.

6세 이하의 어린이에서 Streptococcus mutans와 Streptococcus sobrinus의 분포에 관한 연구

안승태,박재홍,이긍호 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.2

Mutans streptococci는 치아우식증과 관련이 있다고 보고 되고 있으며, 이 streptococci 종 중에서 S. mutans나 S. sobrinus가 인간의 치아우식증에서 가장 일반적으로 나타난다. 치아우식증에서 이 세균종의 분포는 인종과 지역에 따라 다양하다. 본 연구에서는 2-6세인 52명의 치아우식증 환자에서 비우식부위와 우식부위로부터 치태를 채취하여 DNA를 추출한 dex와 gtf 유전자에 기초한 S. mutana와 S. sobrinus에 특이한 primer를 제작한 후 PCR을 시행하여 S. mutans와 S. sobrinus의 분포도를 조사하여 다음과 같은 결과를 얻었다. 1. 어린이 치아우식증 환자에서 S. mutans나 S. sobrinus가 발견된 어린이는 37명 (71.2%)이었다. 2. 이들 어린이 중에서, S. mutans 또는 S. sobrinus가 비우식 부위에서만 발견된 어린이는 3명(5.8%), 우식 부위에서만 발전된 어린이는 22명(42.3%), 두 부위 모두에서 발견된 어린이는 12명(23.1%)이었다. 3. S. mustans나 S. sobrinus가 나타난 비우식부위 치태는 15개 (28.8%), 우식부위 치태는 34개(65.4%)였다. 4. 비우식부위의 경우 S. mutans만 출현한 어린이는 52명 중 8명(15.4%), S. sobrinus만 출현한 어린이는 6명(11.5%), 두 세균종이 같이 출현한 어린이는 1명 (1.9%)이었다. 5. 우식부위에서 S. mutans 출현한 어린이는 52명 중 24명(46.2%), S. sobrinus만 출현한 어린이는 2명(3.8%), 두 세균종이 같이 출현한 어린이는 8명 (15.4%)이었다. 6. S. mutans는 gtf에 기초한 primer, S. sobrinus는 dex에 기초한 primer가 PCR에서 효과적으로 각각의 세균을 검출하였다. 이상의 결과로 미루어 우리나라 어린이의 경우 치아우식증은 MS와 밀접한 연관성이 있으며, MS중에서도 S. mutans가 중요하다고 판단된다. 한편 S. mutans와 S. Sobrinus의 동반출현은 치아우식증과 더욱 밀접한 관련이 있는 것으로 보인다. Mutans streptococci have been reported to be implicated in dental caries. Of these streptococcal species, Streptcoccus mutans and Streptococcus sobrinus are most commonly found in human dental caries. Prevalence of these bacterial species in dental caries is found to be varied in different races and countries. Therefore, importance of these bacteria in dental caries remains to be determined. The present study was performed to detect S. mutans and S. sobrinus in 52 Korean children with dental caries between 2 to 6 years of age. For the study, plaque samples were collected from caries-active(CA) and caries-free(CF) teeth of each subject. DNA was extracted from the plaques and amplified by polymerase chain reaction(PCR) using primers corresponding to dex and gtf genes. The results obtained from the study were as follows: 1. Of 52 children, 37 children (71.2%) were found to harbor S. mutans and/or S. sobrinus. 2. Of the MS-infected 37 children, 3 children (5.8%) harbored S. mutans and/or S. sobrinus in the CF plaques only, 22 children (42.3%) in the CA plaques only and 12 children (23.1%) in both CF and CA plaques. 3. S. mutans and/or S. sobrinus were detected in 34 CA plaques (65.4%), while 15 in CF plaques (28.8%). 4. In the case of CF plaques, 8 plaques (15.4%) were observed to harbor S. mutans only, 6 plaques (11.5%) to harbor S. sobrinus only and 1 plaque (1.9%) to harbor both species. 5. Of CA plaques, 24 plaques (46.2%) were detected to have S. mutans only, 2 plaques (3.8%) to have S. sobrinus only and 8 plaques (15.4%) to have both species. 6. In comparison with the efficiency of two different primers for PCR, it was found that the primer based on gtf gene was more effective in detecting S. mutans, while the primer corresponding to dex gene was better for S. sobrinus. Overall results suggest that MS appears to be important in dental caries of the Korean children, and S. mutans is more closely associated with than dental caries as compared to S. sobrinus.

조기 능동 운동을 가능케 하는 굴건 수복 방법에 관한 생체외 연구

안상로,윤승호 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The results of the flexor tendon repair within the digital sheath have been unpredictable due to peritendinous adhesion. It has been known that three factors are responsible for the formation of adhesion, that is, tendon sheath injury, tendon suture and immobilization. Of these three factors, immobilization is the only one that is theoretically under the control of the surgeon. This problem has led to the development of early active and protective mobilization techniques. However, there was no method providing enough repair strength to a point sufficient to permit early active flexion without risk of tendon rupture or large gap formation. Also the protective mobilization techniques produced a gap at the repair site and could not provide normal amplitude of tendon excursion. So, the author performed these four in vitro studies to obtain a method of flexor tendon repair permitting early active flexion against moderate resistance. The deduced tensile strength permitting early active flexion against moderate resistance is above 7.5kg. The extensor digiti quarti proprius tendon of adult pigs' foreleg was used as the experimental tendon. Each tendon repair was performed under magnification by the Meiji experimental opera tive microscope. The tensile strength in each experiment was tested with a Tensilon tensometer and the gap was measured at each kg. of tensile strength. The first experiment was the measurement of the tensile strength of two strands of 4-0 mersilene with one knot and six strands of 4-0 mersilene with three knots. The second experiment was the measurement of the tensile strength of the diagonal grasp from one grasp to four grasps with a knot, in the tendon end and with a knot far from the tendon end. The third experiment was the measurement of the tensile strength and gap formation of the author's method. Three interrupted sutures with. knots far from the tendon ends were placed in the tendon. Two sutures were placed in both posterolateral aspects of the tendon and one suture was placed in the anterior aspect of the tendon. Each suture grasped only one-sixth of the circumference of the tendon and was placed periph-erally to preserve the intratendinous vasculature. The fourth experiment was the measurement of the tensile strength and gap formation of modified Kessler's, Ketchum's and Savage's method. The following results were obtained. 1. The tensile strength of two strands of 4-0 mersilene with one knot and six srands of 4-0 mersilene with three knots was 2. 45kg and 7.20 kg. respectively which was similar to the deduced tensile strength of 2.5kg. and 7.5kg. respectively. 2. The tensile strength of three and four diagonal grasps with a knot far from the tendon end was 2.83kg. and 2.96kg. respectively which was above the deduced tensile strength of 2.5kg. 3. The tensile strength of the author's method was 8.04kg. which was above the deduced tensile strength of 7.5kg. The gap formation of the author's method with each kg. of tension was low as follows, 0 mm. at 1 kg., 0. 03mm. at 2 kg., 0. 16mm. at 3 kg. 0. 71 mm. at 4 kg. , 1. 53mm. at 5 kg., 2. 38mm. at 6 kg., 3.61mm. at 7 kg., 4.75mm. at 8 kg.. 4. The tensile strength of various methods was low and the gap formation of various methods. was large compared to the author's method. This author's method provided a high tensile strength and a minimal gap, so this method will. be one of the methods that permit immediate active finger flexion against moderate resistance even after tendon softening.

교육용 운전 시뮬레이터의 종합기능 정립에 관한 연구

安勝虎 철도전문대학교 2001 論文集 Vol.16 No.-

In this paper it is tried to create a model of dynamic characteristics for the testing electric locomotive. The complex model, which is based on excel program of the micro soft co., consists of the following components: · model of traction characteristics for EL 8100 · model of recovery braking and air brake for EL 8100 · model of diverse train resistances for EL 8100 - starting and running resistance - curve and gradient resistance · realization of traction characteristics for EL 8100 based on the model of dynamic characteristics · realization of recovery braking characteristics for EL 8100 based on the model of dynamic characteristics.

AISI D2강 위에 다층으로 증착된 WC-Ti_(1-X)Al_(X)N 코팅의 국부부식 거동에 관한 연구

안승호,이정호,김호건,김정구,한전건 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.9

Multilayered WC-Ti_(1-x)Al_(x)N coatings are characterized by periodically repeated structures of lamellae of WC-Ti/WC-Ti_(1-x)Al_(x)N. WC-Ti_(1-x)Al_(x)N coatings of changing Al concentration were deposited on AISI D2 steel by high-ionization sputtered plasma vapor deposition (PVD). The Al concentration could be controlled by using evaporation source for Al and fixing the evaporating rate of other metals. Four kinds of WC-Ti_(1-x)Al_(x)N coatings were prepared: WC-Ti_(0.6)Al_(0.4)N, WC-Ti_(0.53)Al_(0.47)N, WC-Ti_(0.5)Al_(0.5)N and WC-Ti_(0.43)Al_(0.57)N. The corrosion behavior of WC-Ti_(1-x)Al_(x)N coatings was investigated using electrochemical techniques in deaerated 3.5% NaCl electrolyte (galvanic corrosion test, potentiodynamic polarization test, electrochemical impedance spectroscopy and Mott-Schottky method) and surface analyses (GDOES, SEM and EDS). Particular attention was paid to the effect of growth defects on the coating properties related to the corrosion behavior. The results of the coating and substrate galvanic corrosion test showed low galvanic current densities. In the potentiodynamic polarization test and EIS measurement, the corrosion current density of WC-Ti_(0.5)Al_(0.5)N was lower than others and presented higher R_(ct) values than others after 240 h immersion time. This could be attributed to the formation of a Ti oxide, which promotes partial closing of the defects. Capacitance values obtained from EIS as a function of different potentials (C^(-2) vs E) showed that the oxide layer exhibited an n-type semiconductor behavior.