인삼 추출물 함유 한방화장품이 건강한 성인 여성의 삶의 질에 미치는 영향에 관한 예비 연구; Skindex-16을 중심으로

조가영 ( Ga Young Cho ),박효민 ( Hyo Min Park ),권이경 ( Lee Kyung Kwon ),조성아 ( Sung A Cho ),강병영 ( Byung Young Kang ),김윤범 ( Yoon Bum Kim ) 대한화장품학회 2015 대한화장품학회지 Vol.41 No.4

본 연구에서는 건강한 성인 여성을 대상으로 인삼 추출물을 함유한 스킨케어 제품의 시료 제시 방법과 여성들의 삶의 질 개선 효과 간의 상관 관계를 분석하였다. 삶의 질이란 질병이나 건강 상태가 일상생활에 미치는 신체적, 정신적 그리고 사회적 영향에 대한 개인적인 반응을 나타내는 개념이다. 이에 저자들은 45명의 건강한 성인 여성을 두 그룹으로 나누어 한 그룹(Group A)에는 브랜드 고유의 패키지를 유지한 인삼 크림 시료를 제공하였고, 다른 그룹(Group B)은 브랜드와 제품명을 알 수 없는 하얀색 통에 담은 동일한 시료를 제공하여 8주간 사용하게 하였다. 피부 관련 삶의 질 평가는 Skindex-16 설문지를 사용하여 시험 초기와 4주 후, 8주 후에 걸쳐 시행되었다. 시험 참여를 중지한 2명의 피험자를 제외한 43명의 Skindex-16 초기값의 평균은 22.70 ± 4.82점이었다. 8주 후, 그룹 A의 Skindex-16 지표는 초기값 23.30 ± 5.14에서 20.20 ± 4.83으로, 그룹 B는 22.17 ± 4.58에서 20.52 ± 3.60로 통계적으로 유의한 정도로 개선되었다. 세부 항목에서는 그룹 A의 ‘증상’ 항목이 4주 후, ‘감정’ 항목이 8주 후에 통계적으로 유의하게 개선되었으며, ‘기능’ 항목은 어느 그룹에서도 통계적으로 유의한 변화가 관찰되지 않았다. 또한, 총합과 세부 항목 모두 측정 시기 및 그룹에 따른 교호작용은 통계적으로 유의한 차이가 확인되지 않았다. 본 실험을 통해 인삼 크림이 포함된 스킨케어가 건강한 성인 여성의 삶의 질에 긍정적인 영향을 줄 수 있음을 확인하였으며, 스킨케어 행위 자체가 패키지에 대한 영향보다 삶의 질의 개선에 주는 영향이 큼을 추측할 수 있다. 장기적으로 ‘삶의 질’이란 기존의 피부과학 지표의 개선과 주관적 만족도의 개념을 넘어 신규한 화장품 효능 영역으로 추가적인 연구가 필요할 것으로 생각된다. This study is designed to analyze the effect of skincare using cosmetic containing ginseng extract, on improving quality of life (QOL) of healthy women, with blind testing. QOL is a concept that represents how one’s disease or health condition can physically, psychologically, and socially influence his or her daily life. The study was conducted to assess the effect of a ginseng cosmetic preparation on quality of life (QOL) using the Skindex-16 score, stratified by blind versus non-blinded option. 45 healthy women aged between 30 and 49 years with no skin disease were recruited for this study. Volunteers were divided into two groups. Group A (n = 22) received anti-aging cream with ginseng extract in the original packaging, which included the brand name and logo. Group B (n = 23) received the same cream in a plain white jar without any package decoration or logo. Both groups used the cream for 8 weeks. For the skin-related QOL assessment, Skindex-16 was used at baseline, forth, and eighth week. All volunteers except two dropouts in Group A completed the dermatology-specific QOL measure, Skindex-16, at baseline, after 4 weeks, and after 8 weeks of treatment with the provided samples. As a result, the mean score of 43 participants at baseline was 22.70 ± 4.82. There was a significant difference between the baseline score and the score after 8 weeks in both groups: The scores changed from 23.30 ± 5.14 to 20.20 ± 4.83 in Group A, from 22.17 ± 4.58 to 20.52 ± 3.60 in Group B. The “Symptom” subscale of Skindex-16 improved after 4 weeks and the “Emotion” subscale improved after 8 weeks in Group A. The “Function” subscale did not show improvement in either groups. Both groups showed no interaction effect between follow up time and groups in Skindex-16 and subscale. This research opens up the possibility of skincare using ginseng cream having a positive effect on QOL in healthy women. Moreover, one can predict that skincare ritual itself may have greater impact on the improvement of QOL, compared to the product packaging.

The Influence of Feed Energy Density and a Formulated Additive on Rumen and Rectal Temperature in Hanwoo Steers

Cho, Sangbuem,Mbiriri, David Tinotenda,Shim, Kwanseob,Lee, A-Leum,Oh, Seong-Jin,Yang, Jinho,Ryu, Chaehwa,Kim, Young-Hoon,Seo, Kang-Seok,Chae, Jung-Il,Oh, Young Kyoon,Choi, Nag-Jin Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.11

The present study investigated the optimum blending condition of protected fat, choline and yeast culture for lowering of rumen temperature. The Box Benken experimental design, a fractional factorial arrangement, and response surface methodology were employed. The optimum blending condition was determined using the rumen simulated in vitro fermentation. An additive formulated on the optimum condition contained 50% of protected fat, 25% of yeast culture, 5% of choline, 7% of organic zinc, 6.5% of cinnamon, and 6.5% of stevioside. The feed additive was supplemented at a rate of 0.1% of diet (orchard grass:concentrate, 3:7) and compared with a control which had no additive. The treatment resulted in lower volatile fatty acid (VFA) concentration and biogas than the control. To investigate the effect of the optimized additive and feed energy levels on rumen and rectal temperatures, four rumen cannulated Hanwoo (Korean native beef breed) steers were in a $4{\times}4$ Latin square design. Energy levels were varied to low and high by altering the ratio of forage to concentrate in diet: low energy (6:4) and high energy (4:6). The additive was added at a rate of 0.1% of the diet. The following parameters were measured; feed intake, rumen and rectal temperatures, ruminal pH and VFA concentration. This study was conducted in an environmentally controlled house with temperature set at $30^{\circ}C$ and relative humidity levels of 70%. Steers were housed individually in raised crates to facilitate collection of urine and feces. The adaptation period was for 14 days, 2 days for sampling and 7 days for resting the animals. The additive significantly reduced both rumen (p<0.01) and rectal temperatures (p<0.001) without depressed feed intake. There were interactions (p<0.01) between energy level and additive on ruminal temperature. Neither additive nor energy level had an effect on total VFA concentration. The additive however, significantly increased (p<0.01) propionate and subsequently had lower acetate:propionate (A/P) ratios than non-additive supplementation. High concentrate diets had significantly lower pH. Interactions between energy and additive were observed (p<0.01) in ammonia nitrogen production. Supplementation of diets with the additive resulted in lower rumen and rectal temperatures, hence the additive showed promise in alleviating undesirable effects of heat stress in cattle.

Dose-Dependent Effects of Malformin A1 on IAA-Induced Ethylene Production in Mung Bean (Vigna radiata L.) Hypocotyl Segments

( Soon Young Kim ),( A Young Cho ),( Kun Woo Kim ),( Seung Eun Oh ) 한국식물학회 2004 Journal of Plant Biology Vol.47 No.3

Purified malformin Al (cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Ile), a cyclicpentapeptide toxin from Aspergillus niger, was applied to the hypocotyl segments of mung bean (Vigna radiata L.) seedlings to investigate its role in regulating ethylene biosynthesis. Production of ethylene was induced by treating the plants with 0.1 mM indole-3-acetic acid (IAA). When 0.1 μM malformin A1 was then applied, ethylene production increased and the activities of two key enzymes for its biosynthesis, 1-aminocyclopropane-1-carboxylic acid (ACC)-synthase (ACS) and ACC-oxidase (ACO), were also stimulated. However, at levels of 1 or 10 μM malformin A1, both ethylene production and enzymatic activities were significantly reduced. In the case of ACO, in vitro activity was regulated by malformin Al, independent of ACS activity or the influence of IAA. Furthermore, the conjugate form of ACC, N-malonyl ACC, was significantly promoted by treatment with 0.1 μM malformin A1. These data suggest that malformin A1 can modulate ethylene production through diverse paths and that its effect depends on the concentration of the treatment administered.

S-510 A case of peritoneal dialysis-associated peritonitis caused by Agromyces mediolanus

( Ju Hwan Oh ),( Min Woo An ),( Hyun Kwon Ju ),( Na Yun Kang ),( A Young Cho ),( In O Sun ),( Kwang Young Lee ) 대한내과학회 2016 대한내과학회 추계학술발표논문집 Vol.2016 No.1

Introduction:?Agromyces mediolanus (A.mediolanus) is an unusual pathogen in human infection. No case of peritoneal dialysis (PD)-associated peritonitis by A.mediolanus has been reported. This is the first case report of PD-associated peritonitis by A.mediolanus in the world. We describe a case of PD-associated peritonitis by A.mediolanus that was cured after catheter removal.?Case: A 59-year-old man who had been treated with CAPD was admitted to our hospital because of turbid peritoneal effluent accompanied by constant diffuse abdominal pain. The patient had an exit-site infection with purulent secretion. The laboratory findings showed PD peritonitis: the white blood cell (WBC) count of the peritoneal effluents was 1157/mm3 with a neutrophil predominance (91.3%). Cefazolin and ceftazidime were given intraperitoneally per day. The peritoneal WBC decreased, but the patient’s clinical condition didn’t improve on the 5th day after starting intraperitoneal antibiotics. Culture of the peritoneal dialysate revealed Agromyces species, which was susceptible to meropenem, imipenem, vancomycin, but resistant to ceftriaxone. Thus, we changed antibiotics to intraperitoneal vancomycin and meropenem on the 5th day. Despite the intraperitoneal antibiotics for 4 days, the patient’s abdominal pain persisted and the peritoneal WBC count became elevated. Therefore, we removed the PD catheter and the patient was switched to hemodialysis on the 9th day. Vancomycin and imipenem was continued for another 2 weeks and the patient’s clinical condition improved. The patient has been maintained on hemodialysis.?Conclusions: There is no previous report of peritonitis associated with A.mediolanus in a CAPD patient. In this case, we used intraperitoneal cefazolin and ceftazidime and changed to vancomycin and imipenem according to the result of peritoneal dialysate. However the patient improved after removal of catheter. Therefore, early removal of the PD catheter in A. mediolanus related peritonitis without prompt reaction to antibiotic therapy should be considered.

다양한 레진시멘트로 합착한 섬유포스트의 결합강도 비교

이현아,조영곤 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.6

이 연구는 다양한 접착시스템을 사용하는 6가지 레진시멘트를 이용하여 근관에 합착한 섬유포스트를 push-out 검사에 의해 포스트와 치근 상아질 간의 결합강도를 상호 비교하기 위하여 시행되었다. 발거된 하악 소구치의 치관부를 제거하고 치근부를 근관충전한 후 9 mm의 포스트 룸을 형성하였다. 사용된 레진시멘트의 종류에 따라서 무작위로 6개씩 선택하여 Duo-Link 군 Variolink Ⅱ 군, Panavia F 군, Multilink Automix 군, RelyX Unicem 군, Maxcem 군으로 분류하였다. 제조사의 지시에 따라서 각각의 레진시멘트를 Uni-dose needle tip을 이용하여 근단부에서부터 주입하여 광투과성 섬유 포스트인 no. 2 DT Light Post를 근관에 합착하였다. 증류수에 24시간동안 보관한 후, 주수 하에 저속의 Diamond Wheel Saw를 이용하여 각 치근을 횡단면으로 1 mm두께로 절단하여 연속적인 절편 6개를 얻었다. 각 군의 시편을 universal testing machine에 고정하고 push-out 검사를 시행하여 push-out 강도를 통계 분석한 결과, 서로 다른 접착시스템을 사용한 레진시멘트 군 간의 push-out 강도는 유의한 차이가 나타나지 않았다 (p>0.05). The purpose of this study was to compare the push-out strength of a fiber post cemented with various resin cements. Newly extracted 36 human mandibular premolars which had single root canal were selected and their crown portions were removed. The root canal was instrumented using PROTAPER^(TM) system and obturated using continuous wave technique, In each root, a 9-mm deep post space was prepared. #2 translucent fiber post (DT Light post, Bisco Inc., Schaumburg, IL, U.S.A.) was cemented using injection technique with Uni-dose needle tip (Bisco) and six different resin cements. The tested resin cements were Duo-Link(Bisco Inc., Schaumburg, IL, U.S.A.), Variolink Ⅱ (Ivoclar-Vivadent AG, Schann, Liechtenstein), Panavia F (Kuraray Medical Inc., Okayama, Japan), Multilink Automix (Ivoclar-Vivadent AG, Schann, Liechtenstein), RelyX Unicem (3M ESPE Dental Products, St. Paul, MN, U.S.A.), and Maxcem (Kerr Co.,CA, U.S.A.). After storage in distilled water for 24 hours, each root was transversally sectioned into approximately 1-mm thick sections. This procedure resulted in 6 serial sections per root. Push-out test was performed using a universal testing machine (EZ Test, Shimadzu Co.) with a crosshead speed of 1 mm/min. The data were analyzed with one-way ANOVA and Tukey HSD (p=0.05). The push-out strength of the groups which cemented fiber post with Panavia F and Multilink Automix were lower than those of the other groups. But, there were no statistically significant difference among groups at a probability level of 0.05.

순창군 장류로부터 분리된 황국균의 동정 및 특성

임은미 ( Eun Mi Lim ),이지영 ( Ji Young Lee ),모하메드 ( Mohammed A. Abdo Elgabbar ),한갑훈 ( Kap Hoon Han ),이보순 ( Bo Soon Lee ),조용식 ( Yong Sik Cho ),김현영 ( Hyoun Young Kim ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.4

In this study, we attempted to isolate fungi from soybean fermented foods produced in Sunchang County and to identify Aspergillus oryzae from fungal isolates. Ten fungal isolates were identified with β-tubulin gene. According to the sequences of β-tubulin gene, ten fungal isolates were identified as A. oryzae/flavus complex. For further identification of the ten of fungal isolates, omtA gene, one gene of the aflatoxin biosynthesis gene cluster, was sequenced and the sequences were compared with those of A. oryzae and A. flavus strains from the GenBank database. In addition, identification of the ten fungal isolates was further confirmed using the PCR amplicon of norB and cypA intergenic region, in which a deletion was recognized relative to A. flavus and A. parasiticus. The amplicon size of the ten fungal isolate strains was smaller than those of A. flavus and A. parasiticus, but the same as that of the reference A. oryzae strain. These results indicated that the ten isolates should be identified as A. oryzae. The protease activity in rice koji made with 6, 13, 17, 27, 37 and 38 of strain, respectively was twice higher than that in control. The kojis made with nine of the A. oryzae isolates, respectively, did not produce aflatoxin, suggesting that the strains could possibly be used as starters for soybean products.

Comparison of Proteome Components of Helicobacter pylori Before and After Mouse Passage

Lee, Kyoung-Ja,Kim, Bok-Ran,Cho, Young-A,Song, Yun-Gyu,Song, Jae-Young,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Cho, Myung-Je,Rhee, Kwang-Ho,Seo, Ji-Hyun,Youn, Hee-Shang,Lee, Woo-Kon 대한미생물학회 2011 Journal of Bacteriology and Virology Vol.41 No.4

The mouse model is alleged to be a useful tool for understanding of pathophysiological roles of Helicobacter pylori in the development of gastric disorders. However, it has been observed that H. pylori strains significantly differed in their fitness in mice and even mouse strains differed in their susceptibilities to a H. pylori strain. Bacterial components of H. pylori which could affect on its fitness in mice have to be elucidated for the establishment of the mouse model for H. pylori infections. In the comparison of colonization ability between two H. pylori Korean isolates, 51 (isolated from a patient with duodenal ulcer) and 52 (isolated from a patient with gastric cancer), 52 could colonize better than 51 on the gastric mucosa of mouse. Proteome components of H. pylori 52, as a good colonizer and H. pylori 51, as a poor one were quantitatively compared each other. Five bacterial proteins including catalase, urease subunit alpha/beta, enolase and ferritin, were up-regulated in 52. In addition, the respective proteome components of the two strains were also compared with their mouse-passaged homologous strains. Seven and five proteins, which included catalase, flagellin A/B in common, were up-regulated in mouse-adapted 51 and 52, respectively. Among the fourteen identified proteins, urease subunit alpha/beta, flagellin A/B, catalase, ferritin, superoxide dismutase and neutrophil-activation protein have been previously known to be necessary to gastric colonization of H. pylori in animal models. The other up-regulated proteins including enolase, elongation factor Tu and fructose-bisphosphate aldolase have been reported to be associated with acid tolerance of H. pylori. These data provide confirmatory evidence for the importance of those proteins in the development of H. pylori-associated gastric disorders.

Estrogen Receptor-${\alpha}$ 유전자 5' 영역의 Single Nucleotide Polymorphism의 탐색과 한우와 Holstein에서 번식능력 및 산유능력과의 관계

염규태,전향아,박해금,김영신,김현,김재환,성환후,조영무,조재현,고응규,Yeom, Gyu-Tae,Jeon, Hyang-A,Park, Hae-Geum,Kim, Young Sin,Kim, Hyun,Kim, Jae Hwan,Seong, Hwan-Hoo,Cho, Young Moo,Cho, Jae-Hyeon,Ko, Yeoung-Gyu 한국동물번식학회 2014 Reproductive & developmental biology Vol.38 No.3

This study was conducted for SNPs in the 5'-regions of estrogen receptor-${\alpha}$ (ESR-${\alpha}$), and association with calving interval (CI), service per conception (SPC) and 305 days milk yield in Hanwoo and Holstein dairy cattle. The genetic improvement was incurred low reproduction performance. The objective of this study was to investigate connections between single nucleotide polymorphisms (SNP) of Estrogen receptor-${\alpha}$ (ESR-${\alpha}$) with reproduction performance (calving interval, service per conception, and 305 d milk yield) in Hanwoo and Holstein dairy cattle. Hanwoo and Holstein blood samples were collected from 183 and 124 dam of breeding farms and DNA was extracted. Primer design was based on NCBI GenBank (Accession No. AY340579). The PCR-RFLP method with Bgl I was used to genotype the cattle. The result showed two variants of the ESR-${\alpha}$ gene. The Bgl I cut the 492 bp amplification product into 322 bp and 170 bp fragments for allele G, while allele A remained uncut, resulting in two restriction fragments for homozygote G/G and three fragments for heterozygote A/G. We found two of different genotypes in these breeds, A/G and G/G. In Hanwoo, the A/G genotype frequency was 0.13, and G/G was 0.87. The CI of A/G was $382.18{\pm}10.03$ days, and G/G was $381.69{\pm}5.22$ days. The SPC of A/G was $1.62{\pm}0.16$, and G/G was $1.32{\pm}0.04$. While CI showed no significance difference, SPC exhibited significant difference (p<0.05). In Holstein cattle, the frequency of genotype A/G was 0.02 and G/G was 0.98. The 305 days milk yield of A/G was $7,253.00{\pm}936.00kg$ and of G/G was $8,747.51{\pm}204.88kg$, showing no significant difference.

Measurement of Neutron Total Cross Section and Resonance Parameters of Natural Hafnium at Pohang Neutron Facility

A. K.M.M.H. Meaze,Guinyun Kim,Dongchul Son,Hengsik Kang,Hossain Ahmed,In Soo Ko,Jong Hwa Chang,Kun Joong Yoo,Moo-Hyun Cho,Tae-Ik Ro,Won Namkung,Won-Chung Chung,Young Seok Lee,Young-Ae Kim 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.2

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북한 이탈주민의 외상후 스트레스장애에 대한 3년 추적연구

홍창형,유정자,조영아,엄진섭,구현지,서승원,안은미,민성길,전우택 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objective : This study is a follow-up of a first survey of 200 North Korean defectors who entered South Korea in 2001. The purpose of this study is to examine changes in the current prevalence of partial PTSD and full PTSD after 3 years and to evaluate related factors. Methods : This study followed up on 151 of the 200 North Korean defectors from our first survey. 20 interviewers conducted face-to-face interviews and assisted defectors in completing a self-report assessment. The traumatic events which participants with chronic PTSD and delayed onset PTSD experienced were then examined. Results : Over the 3 years, the current prevalence rate of partial PTSD was reduced from 31.8% to 5.3%, and the current prevalence rate of full PTSD was reduced from 27.2% to 4.0%. Of particular interest was that 88.8% of those diagnosed with full or partial PTSD in the first survey had recovered after 3 years. Conclusion : The current prevalence rate of partial and full PTSD rapidly decreased during 3 years.