벤조산과 관련 화합물의 구강암 세포주에 대한 세포독성효과

한두석,박윤규,강정일,백승화,Han, Du-Seok,Park, Yeun-Kju,Kang, Jeong-Il,Baek, Seung-Hwa 대한약학회 2008 약학회지 Vol.52 No.4

The cytotoxic effect of benzoic acid and related compounds on the growth of normal cell lines and human oral epithelioid cell line was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT) methods. 3,4,5-Trihydroxybenzoic acid decreased the cell viability of human oral epithelioid cells and the cell adhesion activity of human oral epithelioid cells. Under the light microscopy, 100 ${\mu}M$ 3,4,5-trihydroxybenzoic acid showed the highest cytotoxic activity. From these results, we can propose that 3,4,5-trihydroxybenzoic acid has a potential anticancer activity.

Carbofuran이 NIH 3T3섬유모세포와 흰쥐의 신장에 미치는 영향과 Phenobarbital sodium의 보상효과에 관한 연구

한두석,임요섭,한성수 한국독성학회 1997 Toxicological Research Vol.13 No.1

This study was carried out to investigate toxicity of insecticide carbofuran and compensatory effects of phenobarbital sodium (PB) in vivo and in vitro. Sprague Dawley male rats were used as experimental animals and divided into carbofuran only administered group and simultaneous application group of carbofuran and PB. At 30 rain and 1, 3, 6, 12, 24, 48 and 96 hrs after each treatment, the animals were sacrificed by decapitation. Kidney were immediately removed, immersed in fixatives, and processed with routine method for light microscopic study. Paraffin sections were stained with H-E, PAM and PAS. $5.0\times 10^4$ cell/ml of NIH 3T3 fibroblast in each well of 24 multidish were cultured: After 24 hours, the cells were treated with solution of six groups; control group cultured in media only, carbofuran $MTT_50$ or $NR_50$ group cultured in the media containing carbofuran $MTT_50$ or $NR_50$ and four experimental groups cultured in the media containing carbofuran $NR_50$ plus various concentratins of PB. After the NIH 3T3 fibroblast of all groups were cultured in same condition for 48 hours, Tetrazolium MTT (MTT) and NR (neutral red) assay were performed to evaluate the cytotoxicity of cell organelles. Under the light microscope, atrophic change of renal corpuscles were frequently observed in 1 and 2 days after carbofuran treatment. The increase of the mesangium was apparent in 1 and 2 days after carbofuran treatment. Necrotic changes of the epithelium and loss of brush border of proximal tubules were most severe at 2 and 3 days after carbofuran treatment, respectively. In contrast, there were no evidences of the toxic effects on renal tissues at 48hrs in carbofuran-PB treated groups. Carbofuran $MTT_50$ and $NR_50$ were 78$\mu M$, 82.5$\mu M$ respectively. MTT and NR quantities were significantly increased in carbofuran-PB 100$\mu M$ treatment group and carbofuran-PB 100$\mu M$ treatment group. On the basis of these results, it is obvious that PB has compensatory effects against carbofuran toxicity.

한국산 생약으로부터 해독물질의 개발(제3보) 카드뮴으로 처리한 3T3섬유모세포에서 소엽 메탄올 분획의 수복효과

한두석,유현경,곽정숙,백승화 한국독성학회 1995 Toxicological Research Vol.11 No.2

This study was carried out to evaluate the cytotoxicity of cadmium on 3T3 fibroblast and to develop the antidote on 3T3 fibroblast which was injuried by $IC_{50}$ of cadmium. The groups for repaired effects were divided into 7 groups such as medium alone treated group. Cadmium treated $IC_{50}$ groups and 5 experimental groups $(IC_{50}$ cadmium plus $10^{-4}$ concentration of each methanol fraction). After incubation for 48 hrs in the same conditions, MTT (tetrazolium MTT), NR (neutral red) and SRB (sulforhodamine B protein) assay were measured. Light microscopic observations were also investigated. The ethyl acetate fraction of Perilla frutescens showed significantly repaired effect against cadmium cytotoxiclty and this fraction inhibited critical cell regeneration in light microscopy.

페놀산의 구조가 암세포에 대한 세포독성에 미치는 영향

한두석,오상걸,오은상 한국독성학회 2003 Toxicological Research Vol.19 No.1

The purpose of this study was to determine the role of substituted groups in phenolic compounds to develop an anticancer agent having strong cytotoxicity against cancer cells but weak against normal cells. The phenolic compounds used in this study were gallic acid and ferulic acid with hydroxyl and carboxyl groups, syringic acid with hydroxyl, carboxyl and methoxy groups, and pyrogallol with hydroxyl groups. Cytotoxicities of these compounds were evaluated by MTT assay for cell viability and XTT assay for cell adhesion activity in normal human skin fibroblast (Detroit 551) and human skin melanoma (SK-MEL-3) cells. Syringic acid, gallic acid and ferulic acid decreased the cell viability and cell adhesion activity in SK-MEL-3 cells but not in Detroit 551 cells while pyrogallol decreased in both cells. The susceptibility of cell viability based on the IC_(50) values of MTT assay in Detroit 551 cells was in the following order: pyrogallol > gallic acid > ferulic acid > syringic acid, while it was in SK-MEL-3 cells: Syringic acid > progallol > ferulic acid > gallic acid. These results suggest that carboxyl and methoxy groups of these compounds play an important role in selectivity of cytotoxicity in normal and cancer cells.

웨이트 트레이닝 모델에 의한 운동부하가 흰쥐 장단지근의 핵소체 형성부위 활성과 근섬유의 형태에 미치는 효과

한두석,천상우,최영자 원광대학교 생체재료·매식연구소 1995 원광생체재료·매식 Vol.4 No.1

This study was to investigate the nucleolar organizer region(NOR) associated protein by means of silver chloride staining and muscle fiber types in sections stained for SDH in the gastronecmius muscle loaded with movement by klitgaard's weight training model. Ag-NOR technique is a direct measure of the ongoing transcriptional activity of the rRNA gene or their activity. The number of Ag-NORs sites within nucleolus may reflect protein synthetic activity and proliferation. Thirty Spraque-Dawley adult female rats(190-200g) were divided into control and 3 experimental groups, which were trained for 1, 2 and 3 months. Body weight and muscle weight measured at 1, 2 and 3 months. The removed muscle was fixed by immersion, and processed for light microscopy. The results were as follows; 1. Body weight was increased, but muscle weight was decreased with the lapse of time of training. 2. After movement, the mean number of nucleolar organizer regions per nucleus was significantly increased at 2 and 3 months in gastronecmius muscle. 3. The percent of nucleolus include 4 Ag-NOR or more was increased with the lapse of time of training. 4. In muscle fiber types, type A was decreased but type C was significantly increased with the lapse of time of training.

팔선양조용수의 카드뮴에 대한 세포독성 억제효과

한두석,김진석,한종현,이호섭,김지주,최은영,강길웅,백승화 圓光大學校 基礎自然科學硏究所 2000 基礎科學硏究誌 Vol.18 No.1

카드뮴, E. coil endotoxin 과 Salmonella endotoxin이 NIH3T3 섬유모세표에 미치는 세포독성을 검정하고, 카드뮴 MTT_50에 의하여 손상된 NIH3T3 섬유모세포를 해독시킬수 있는 물질을 창출할 목적으로 본 연구를 수행하였다. 실험은 배양액만으로 배양한 군을 대조군, 세포독성실험에 의하여 결정된 IC_50농도의 카드뮴과 4종류의 팔선양조용수를 각가 배양액에 넣어 배양한 군을 실험군으로 분류하여 실험하였다. 모든 군은 동일한 조건에서 48시간 배양한 후, MMT량을 측정하고 광학현미경적 관찰을 실시하였다. 팔선양조용수는 E. coil endotoxin에 대하여 해독효과가 높게 나타났고, 카드뮴과 Salmonella endotoxin에 대하여도 10^-2㎎/㎖ 농도와 10^-3㎎/㎖ 농도에서 유의한 해독효과를 나타냈다. This study was carried out to evaluate antitoxic effects PULSUN brewing water against cadmium by colorimetric methods. The antitoxic activity of PULSUN brewing water in NIH 3T3 fibroblasts was evaluated by MTT{3-(4,5-dimethyIthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide}assay. The light microscopic study was carried out to observe morphological changes of the treated cells. These activity E. Coli endotoxin and salmonella antitoxin. The number of NIH 3T3 fibroblasts were antioxic and tend to recpnerate. These results suggest that PULSUM brewing water retains a potential antitoxic activity.

농길리 메탄올 추출물의 세포독성

한두석,정우영,박명오,신민교,오현주,백승화,Han Du-Seok,Chung Woo-Young,Park Myung-Oh,Shin Min-Kyo,Oh Hyun-Ju,Baek Seung-Hwa 대한예방한의학회 2001 대한예방한의학회지 Vol.5 No.1

The cytotoxic activity of Cratalariae sessiliflorae on cultured NIH 3T3 fibroblasts and human oral epithelioid carcinoma cells (KB) were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) colorimetric method These fractions of methanol extract of Cratalariae sessiliflorae showed inhibitory effect in vitro in the milligram range against KB cell lines. In general, the antitumor activities of these fractions were does-dependent over the milligram range. The comparison of IC50 values of these fractions in tumor cell lines showed that their susceptibility to these fractions decrease in the following order: Fr. 4> Fr. 6> Fr. 10> Fr. 2> Fr. 11> Fr. 3> Fr. 8> Fr. 7> Fr. 9> Fr. 1> Fr. 5 by the MTT assay. These fractions were tested for their cytotoxic effects on NIH 3T3 fibroblasts using MTT assay. They exhibited potent cytotoxic activities in vitro in the milligram range against NIH 3T3 fibroblasts. In general, the cytotoxic activities of these fractions were does-dependent over the milligram range. The comparison of CD50 values of these fractions in NIH 313 fibroblasts shows that their susceptibility to these fractions in decrease the following order: Fr. 10> Fr. 9> Fr. 2 = Fr. 4> Fr. 8> Fr. 11> Fr. 1 = Fr. 7> Fr. 3> Fr. 5 = Fr. 6 by the MTT assay. These results suggests that fraction 5 has the most growth - inhibitory activity against KB cell lines.

운동부하가 장단지근의 수축기능과 구조에 미치는 영향

한두석,최영자 원광대학교 생체재료·매식연구소 1997 원광생체재료·매식 Vol.6 No.2

授乳期間中 家兎 乳腺組織內 大식細胞의 動態에 關한 形態學的 觀察

韓斗錫 圓光大學校 1986 論文集 Vol.20 No.2

本 硏究는 E. coli를 注入한 家兎의 乳腺組織內에서 大? 細胞의 動態를 觀察하기 위하여 實施하였다. 乳腺組織의 病理學的 所見은 E. coli를 注入한 후 經時的으로 觀察하였으며, 大 細胞의 動態는 E. coli를 注入하여 炎症이 惹起된 乳腺內에 Trypan blue와 indian ink를 注入한 후 觀察하였다. 그 결과는 다음과 같았다. E. coli의 浮游液을 注入한 群에서는 共히 肉眼的으로 乳腺의 腫?, 充血 및 出血, 또한 限局性 壞死?와 結이 있었다. 組織學的으로는 初期에 腺胞와 乳管內에 多數의 好中球의 出現과 上皮細胞의 變成, 壞死 및 脫落], 間質內에 好中球의 浸潤, 出血 및 水腫이 觀察되었다. 時間이 經過함에 따라 ??芽細胞의 增殖과, 淋巴球, 好?球 및 大 細胞의 出現이 있었으며, 동시에 이들 細胞成分으로 構成된 限局性 壞死?가 觀察되었다. 肉芽腫性 變化가 7일째부터 認定되었다. 健康한 家兎의 授乳末技 乳腺組織의 綜合組織內에서는 授乳初期의 乳腺組織에 比하여 Trypan blue와 indian ink 粒子를 ?食한 大 細胞가 多數 觀察되었다. 그리고 炎症初期의 炎症? 周圍에서는 大 細胞를 少數를 認定할 수 있었으나 炎症이 進行됨에 따라 多數의 大 細胞가 炎症? 周圍에 出現하였다. The present study was undertaken to clarify the migration of macrophage in the mammary gland of rabbits with E. coli strains. The pathological changes were observed in mammary gland experimentally induced with E. coli strains and the migration of macrophage after injection of trypan blue or ink was examined in the mammary glands of healthy lactating rabbits and of the experimentally infected rabbits. From the results reported, the following points are ocncluded. In the bacterial suspension infused by E. coli, the affected quarters of udder showed grossly swelling, hyperemia, hemorrhage, focal necrosis ad firmnes. The microscopic findings of early stage of the mammary gland were appearance of large numbers of heterophils in the glandular lumina and ducts accompanied by degeneration, necrosis and desquamation of epithelial cells, and also infiltration of heterophils, hemorrhage and edema in the interstitial tissue, and destruction of alveoli. later, proliferation of fibroblasts, plasma cell, lymphocytes, eosinophils and macrophages appeared in the glandular tissue, and with these cells necrotic foci of glandular tissue were surrounded by highly proliferated connective tissue. in addition, glanulomatous inflammatory changes could be observed in the glandular tissue from the 7th day after infusion. Macrophages phagocytized the particles of trypan blue or indian ink were increased in number in the glandular tissue of early lactating period as compared with the later lactating period in the normal rabbits. also numerous macrophages appeared around the inflammatory foci in the course of advance of inflammation induced by bacterial suspension.

배양 섬유모세포에서 6가 크롬의 세포독성에 대한 Poncirin의 영향

한두석,전성우,양승진,최병남,석승한,홍기연,송호준 대한본초학회 2006 大韓本草學會誌 Vol.21 No.1