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Bacillus licheniformis EMR-1에서의 MLS 유도내성 기전 -erm K의 크로닝-
최응칠,곽진환,B.와이스브럼,Choi, Eung-Chil,Kwak, Jin-Hwan,Weisblum, Bernard 대한약학회 1988 약학회지 Vol.32 No.4
Inducible MLS resistance gene of Bacillus licheniformis specified by erm K was subcloned in Bacillus subtilis and the DNA sequence corresponding to its control region was determined. The determinant erm K was in Pvu II=Hind III fragment, which was 1.3 kb. The leader region is capable of forming a complex series of inverted complementary repeat sequences (ICRS) centering on at least six axes of symmetry, some of them mutually exclusive, in a way that resulted ultimately in post-transcriptional unmasking of the ribosome loading site for methylase synthesis.
세균의 항생물질 내성에 관한 연구 Macrolide계 항생물질에 대한 유도 내성 Bacillus속 세균
최응칠,김병각,심미자,정경수,김혜령,이종길 대한약학회 1982 약학회지 Vol.26 No.3
Several strains of bacteria having resistance to macrolide antibiotics were isolated. EMR-1, one of them, exhibited the induced resistance to macrolide antibiotics and this microorganism was identified as a bacterium belong to Bacillus species. The subinhibitory concentration of erythromycin or oleandomycin induced strong resistance to both erythromycin and oleandomycin themselves and to other macrolide antibiotics such as leucomycin, spiramycin and josamycin. The effective concentration of inducer, erythromycin was $0.0016-0.2\mu$g/ml. The inactivating enzyme of these antibiotics was not produced by EMR-1.
최응칠 생화학분자생물학회 1986 BMB Reports Vol.13 No.4
Viomycin inhibited polypeptide biosynthesis, initiation complex formation and translocation of peptidyl-tRNA on ribosomes derived from a sensitive strain of Mycobacterium smegmatis (R-15), but not significantly on ribosomes from viomycin-resistant mutants (R-31 and R-43). The inhibition of translocation was stronger than that of initiation complex formation in the sensitive strain. The binding of [^(14)C] tuberactinomycin O, a viomycin analog, to ribosomal particles was studied by Millipore filter method, The sensitive ribosome exhibited higher affinity for the antibiotic than the resistant ribosomes, The resistance was localized on the largeribosomal subunit in a mutant (R-31), and on the small subunit in another mutant (R-43). The binding of the drub; to the sensitive ribsomal subunit was markedly reduced by combination with the resistant pair subunit, and the entire ribosome became resistant to the antibiotic.
Mycobacterium smegmatis를 이용한 Viomycin의 내성 및 작용 기전에 관한 연구
최응칠 대한약학회 1980 약학회지 Vol.24 No.1
Viomycin inhibited polypeptide biosynthesis, initiation complex formation and translocation of peptidyl-tRNA on ribosomes derived from a sensitive strain of Mycobacterium smegmatis (R-15), but not significantly on ribosomes from viomycin-resistant mutants(R-31 and R-43). The inhibition of translocation was stronger than that of initiation complex formation in the sensitive strain. The binding of [$^{14}C$] tuberactinomycin O, a viomycin analog, to ribosomal particles was studied by Millipore filter method. The sensitive ribosome exhibited higher affinity for the antibiotic than the resistant ribosomes. The resistance was localized on the large ribosomal subunit in a mutant(R-31), and on the small subunit in another mutant(R-43). The binding of the drug to the sensitive ribosomal subunit was markedly reduced by combination with the resistant pair subunit, and the entire ribosome became resistant to the antibiotic.
a possible combined attenuation control of the inducible MLS resistance
최응칠,Weisblum, B. 한국미생물학회 1987 微生物과 産業 Vol.13 No.2
이글에서는 항생물질 내성의 생화학적 기전, MLS계 항생물질 내성기전, Post-transcriptional attenuation control, MLS계 항생물질 내성기전 연구방향, erm K의 cloning: transcriptional attenuation control의 가능성에 대해 논하였다.
한국산 생약자원 개발연구 (제2보) : prunus tomentosa의 약효성분에 관하여
최응칠(Eung-Chil Choi),한대석(Dae-Suk Han),김영호(Young-Ho Kim),홍인혁(In-Hyuk Hong) 한국생약학회 1983 생약학회지 Vol.14 No.2
HPLC is a useful method for identifying amygdalin which has been the subject of considering controversy for cancer remedy. The resolution of amygalin was satisfactory and the separation of amygdalin was accomplished using carbohydrate analysis column, mobile phase of acetonitrile: H₂O (90 : 10), and UV detector. Quantitative analysis showed that amygdalin contents of Prunus tomentosa, P. serrulata, and P. ishidoyana(Amygdalaceae) were 2.18, 2.41, and 2.26%, respectively. It was suggested that the seed of Prunus species was worth useful resource of amygdalin.