Sweet BV의 성분분석과 항체역가 및 allergy 반응에 대한 임상적 연구

최석호,차배천,권기록,Choi, Suk-Ho,Cha, Bae-Chun,Kwon, Ki-Rok 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.2

Objectives : The aim of this study was to observe prevention of allergic reactions of Sweet Bee Venom (removing enzyme components from Bee Venom). Methods: Content analysis of Sweet Bee Venom and Bee Venom was rendered using HPLC method and characterization of Anti-Sweet Bee Venom in Rabbit Serum. Clinical observation was conducted for inducement of allergic responses to Sweet BV. Results : 1. Analyzing melittin content using HPLC, Sweet BV contained 34.9% more melittin than Bee venom pharmacopuncture at same concentration. 2. Observing chromatogram of HPLC, removal of the enzyme was successfully rendered on Sweet BV. 3. The anti-serum of Sweet BV showed high titers against melittin and bee venom and relatively low titer against phospholipase A2. 4. After conducting approximately 3,000 cases of Sweet BV administration, not a single case of generalized anaphylatic reaction occurred in clinical observation. 5. Mild compared to the bee venom pharmacopuncture, Sweet BV showed some acute hypersensitive reactions of edema, itchiness, and aching locally. 6. Sweet BV was administered on six patients with previous history of suffering from generalized acute hypersensitive reactions with the bee venom. None of the patients showed allergic reactions with Sweet BV, suggesting it can effectively prevent anaphylatic shock which may occur after the bee venom pharmacopuncture procedure. Conclusion : Summarizing above results, Sweet Bee Venom appears to be an effective measurement against allergic reactions from the bee venom pharmacopuncture especially against anaphylatic shock.

왕고들빼기로부터 얻은 Triterpene Acetate의 혈청 콜레스테롤 저하효과

김미정,이은,차배천,최무영,임태진,박희준,Kim, Mi-Jeong,Lee, Eun,Cha, Bae-Chun,Choi, Moo-Young,Rhim, Tae-Jin,Park, Hee-Juhn 한국생약학회 1997 생약학회지 Vol.28 No.1

The diets with three types of triterpenoid constituents, which were isolated from Lactuca indica, provoked significant changes of serum lipoprotein-cholesterol meta bolisms of hypercholesterolemic rats induced by high-cholesterol diet, together with the reduction of atherogenic index. Especially, triterpene acetates which have triterpene moieties such as ${\beta}-amyrin$, ${\alpha}-amyrin$, lupeol, pseudotaraxasterol, taraxasterol and germanicol showed a considerable hypocholesterolemic activity. The rat given orally with triterpene acetates did not exhibit a significantly higher value of atherogenic index than that of normal rats.

인삼과 산양삼, 산삼의 HPLC를 이용한 부위별 성분 분석 비교

한영주,권기록,차배천,권오만,Han, Young-Ju,Kwon, Ki-Rok,Cha, Bae-Chun,Kwon, Oh-Man 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.1

Objectives : The aim of this experiments is to provide an objective differentiation of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng through components analysis of different parts of ginseng. Methods : Comparative analyses of ginsenoside-$Rg_3$, ginsenoside-$Rh_2$, and ginsenosides $Rb_1$ and $Rg_1$ from the root, stem, and leaves of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng were conducted using HPLC. Results : 1. For content comparison of leaves, ginseng showed highest content of ginsenoside $Rg_1$ than other samples. Natural wild ginseng showed relatively high content of ginsenosides $Rg_1$ and $Rb_1$ than other samples. 2. For content comparison of the stem, ginseng and 10 years old Chinese cultivated wild ginseng didn't contain ginsenoside $Rb_1$. Natural wild ginseng showed higher content of ginsenosides $Rg_1$ and $Rb_1$ than other samples. 3. For content comparison of the root, ginsenoside $Rh_2$ was found only in 5 and 10 years old Korean cultivated wild ginseng. 4. Distribution of contents by the parts of ginseng was similar in ginseng and Chinese cultivated wild ginseng. Conclusions : Above experiment data can be an important indicator for the identification of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng.

HPLC를 이용한 인삼, 홍삼, 산양산삼 및 홍산삼의 성분 비교 분석

이장호,권기록,차배천,Lee, Jang-Ho,Kwon, Ki-Rok,Cha, Bae-Chun 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.2

Objectives The aim of this experiment is to provide an differentiation of ginseng, red ginseng, cultivated wild ginseng(CWG), and red wild ginseng(RWG) through component analysis using HPLC(High Performance Liquid Chromatography, hereafter HPLC). Methods Comparative analyses of ginsenoside $Rg_3$, ginsenoside $Rh_2$, and ginsenosides $Rb_1$ and $Rg_1$ of various ginsengs were conducted using HPLC. Results 1. CWG was relatively heat-resistant and showed slow change in color during the process of steaming and drying, compared to cultivated ginseng. 2. Ginsenoside $Rg_3$ was not detected in cultivated ginseng and CWG, whereas it was high in red ginseng and RWG. Ginsenoside $Rg_3$ was more generated in red ginseng than in RWG. 3. Ginsenoside $Rh_2$ appreared during steaming and drying of cultivated ginseng, whereas it was more increased during steaming and drying of CWG. 4. Ginsenoside $Rg_1$ content was more increased during steaming and drying of cultivated ginseng, whereas it was more decreased during steaming and drying of CWG. 5. Ginsenoside $Rb_1$ content was increased about 500% during steaming and drying of cultivated ginseng, whereas it was increased about 30% during steaming and drying of CWG, indicating that ginsenoside $Rb_1$ was more generated in red ginseng than in RWG. 6. Ginsenoside $Rg_3$ content was higher, whereas ginsenoside $Rg_1$ content was lower in 11th RWG than in 9th RWG, indicating that ginsenoside $Rg_3$ content was increased and $Rg_1$ content was decreased as steaming and drying continued to proceed. Ginsenoside $Rh_2$ and $Rb_1$ contents began to be increased, followed by decreased after 9th steaming and drying process. Conclusions Above experiment data can be an important indicator for the dentification of ginseng, red ginseng, CWG, and RWG. And the following studies will be need for making good product using CWG.

월별 봉독의 함량 비교 분석

곡경승,이장호,김민기,차배천,권기록,Chu, Ching-Seng,Lee, Jang-Ho,Kim, Min-Ki,Cha, Bae-Chun,Kwon, Ki-Rok 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objectives : The aim of this study was to observe variation of Bee Venom content from the collection period. Methods : Content analysis of Bee Venom was rendered using HPLC method by standard melittin Results : Analyzing melittin content using HPLC, 478.97mg/g at june , 493.89mg/g at july, 468.18mg/g at August and 482.15mg/g was containing in Bee Venom at september. So the change of melittin contents was no significance from June to September. Conclusion : Above these results, we concluded carefully that collecting time was not important factor for the quality control of Bee Venom, restricted the period from June to September.

Sweet BV의 함량분석과 시술 부위별 LD⁵⁰ 관찰

곡경승,박희수,김민기,차배천,이은,권기록,Chu, Ching-Seng,Park, Hee-Soo,Kim, Min-Ki,Cha, Bae-Chun,Lee, Eun,Kwon, Ki-Rok 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objectives : This study was conducted to carry out quantitative evaluation and safety of Sweet Bee Venom. Methods : Content analysis was done using HPLC, measurement of LD$^{50}$ was conducted intravenous, subcutaneous, and intramuscular injection to the ICR mice. Results : 1. According to HPLC analysis, removal of the enzymes containing phospholipase A2 was successfully rendered on Sweet Bee Venom. And analyzing melittin content, Sweet Bee Venom contained 12% more melittin than Bee Venom. 2. LD$^{50}$ of ICR mice with Sweet Bee Venom was more than 20mg/kg in subcutaneous injection and intravenous injection, between 15mg/kg and 20mg/kg in muscular injection. 3. LD$^{50}$ of ICR mice with Bee Venom was between 6 and 9mg/kg in subcutaneous injection and intravenous injection, and more than 9mg/kg in muscular injection. Conclusion : Above results indicate that Sweet Bee Venom was more safe than Bee Venom and the process of removing enzymes was well rendered in Sweet Bee Venom.

처치온도에 따른 초미분화 산양삼의 홍삼화 연구

김진호,권기록,이은희,차배천,Kim, Jin-Ho,Kwon, Ki-Rok,Lee, Eun-Hee,Cha, Bae-Chun 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.3

Objectives : The aim of this study was to find optimal conditions for producing red ginseng from cultivated wild ginseng using the Turbo Mill. Methods : Characteristics of powdered cultivated wild ginseng based on various temperature settings of the Turbo Mill were observed, and changes in the content was measured by HPLC for various ginsenosides. Results : 1. The diameter of cultivated wild ginseng powder ground by the Turbo Mill was around 10${\mu}m$. 2. As the temperature rose, pressure, Specific Mechanical Energy(SME), and density decreased, whileas Water Solubility Index(WSI) increased. 3. As the temperature rose, super fine powder showed tendency to turn into dark brown. 4. Measuring content changes by HPLC, there was no detection of ginsenoside Rg3 and ginsenosideRg1, Rb1, and Rh2 concentrations decreased with increase in temperature. Conclusions : Super fine powder of cultivated wild ginseng produced by the Turbo Mill promotes easy absorption of effective ingredients by breaking the cell walls. Using this mechanism to produce red ginseng from cultivated wild ginseng, it yielded less than satisfactory results under the current experiment setup. Further researches are needed to verify more suitable condition for the production of red ginseng.

GC - MS Analysis of Diterpene Quinone Constituents of Salviae Miltiorrhizae Radix and Biological Activity

박희준(Hee-Juhn Park),이승배(Seung-Bae Lee),이은(Eun Lee),차배천(Bae-Chun Cha),최무영(Moo-Young Choi),이승목(Sung Mok Lee),정원태(Won Tae Chung) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.3

단삼의 혈소판응집 억제 활성물질이 지용부 및 수용부에 분포한다는 각각 다른 보고가 있으나 본 연구에서는 수용부는 혈소판응집억제 활성을 나타내지 않았으며 diterpene quinone 성분의 하나인 cryptotanshinone은 현저한 혈소판응집억제 활성을 나타내었으므로 이의 유효 생리활성물질은 diterpene quinone 색소에 분포할 것으로 추측된다. 뿐만 아니라, cryptotanshinone은 항균작용도 동시에 나타내는 생리활성 물질이었다. 또한, 복잡한 조성을 나타내는 단삼의 지용성 색소 성분의 분석을 위해서 GC-MS 분석을 행한 결과 유용한 분석적 결과를 제시하였다. 즉, 가장 많은 색소 성분은 tanshinone Ⅱ이었으며, 또한 새로운 diterpene quinone 성분의 존재가 시사되었다. The ether extract of Salviae miltiorrhizae Radix(SMR) was fractionated to give five subfractions, so that two subfractions of them were recrystallized to yield each pure diterpene quinone pigment. On the basis of spectral evidence, these two compounds were identified as tanshinone Ⅱ and cryptotanshinone. Cryptotanshinone exhibited both of a potent platelet anti-aggregating activity in vitro and a potent antimicrobial activity. GC-MS analysis of the ether extract showed that tanshinone Ⅱ was contained in the largest proportion of all the diterpene quinones. In addition, GC-MS analysis gave other valuable analytical informations.

해동피의 화학성분 및 생리활성

이은(Eun Lee),최무영(Moo Young Choi),박희준(Hee Juhn Park),차배천(Bae Chun Cha),조순현(Soon Hyun Cho) 한국생약학회 1995 생약학회지 Vol.26 No.2

The study on phytochemical analysis and the biological activity of Kalopanacis Cortex was carried out in this research. As a phytochemical result, liriodendrin as a lignan glycoside was isolated and characterized. Two subfraction separated from the acidic substance of CHCl₃ fraction were saturated and unsaturated fatty acid, respectively. Saturated fatty acid mixture identified from GC-MS tool was as follows: palmitic acid, stearic acid, arachidic acid, heneicosanoic acid, docosanoic acid, tricosanoic acid, tetracosanoic acid, pentacosanoic acid, hexacosanoic acid and octacosanoic acid. Unsaturated fatty acid was found to be linoleic acid on the basis of spectroscopic method. An active principle of liriodendrin exhibited significant antihepatotoxic activity but failed to show a considerable antiedemic activity. In this paper, the result of writhing test on liriodendrin was also described.

왕고들빼기의 혈청 콜레스테롤 저하효과 및 트리테르페노이드 성분

박희준(Hee Juhn Park),이명선(Myung Sun Lee),이은(Eun Lee),최무영(Moo Young Choi),차배천(Bae Chun Cha),정원태(Won Tae Jung),양한석(Han Suk Yang) 한국생약학회 1995 생약학회지 Vol.26 No.1

A methanol extract of the herbs of Lactuca indica L. effectively decreased the serum levels of total cholesterol and LDL-cholesterol when orally administered with diet. On fractionation of the extract, a chloroform-soluble fraction showed the similar effects with the methanol extract. Chromatographic separation afforded a miarture of triterpene alcohols and their acyl derivatives. A mixture of triterpene alcohols were identified as β-amyrin, α-amyrin, lupeol, pseudotaraxasterol, taraxasterol and germani col on the basis of spectroscopic methods. The acyl moieties in the corresponding acyl mixture were characterized as acetates and palmitates, respectively. And three kinds of sterol such as β-sitossterol, compesterol and stigmasterol were isolated as a mixture state.