웅성불임 Burley 종 담배에 있어서 이면교배에 의한 양적형질의 유전분석

정윤화,제상율 한국육종학회 1982 한국육종학회지 Vol.14 No.1

연초의 이배체와 반수체의 저온단일 감응성 비교

정윤화,금완수 한국연초학회 1994 한국연초학회지 Vol.16 No.2

Response of two ploidy levels to cool temperature and short day treatment were investigated under controlled conditione of Phytotron. The haploid and diploid of seven genotypes were started and grown to the 8- leaf stage in the greenhouse. They were treated during 15 and 20 days to 8- hour photoperiods at 18$^{\circ}C$ in controlled - environmental room to induce premature flowering, respectively. Diploid plants of seven genotypes flower later than their haploid plants at 20 days treatment. But under 15 days treatment, diploid plants of NC82, Hicks, BY4, NC2326 and Coker86 were not different from their haploid plants for days to flower. Diploid plants of seven genotypes developed the same number of leaves as their haploid plants at 20 days treatment. Under 15 days treatment, diploid plants of Coker347 and NC95 developed more leaves per plant than their haploid plants. Correlation coefficient between the ranks of leaves per plant of seven genotypes at two ploidy levels was 0.964 and 0.929 at 15 and 20 days treatment, respectively.

연초(Nicotiana tabacum L.)의 반수체 육종법 1주1계통법, 집단육종에 의한 육성계통의 특성비교

정윤화,이승철,김달웅 한국연초학회 1992 한국연초학회지 Vol.14 No.2

The present study was conducted to compare the relative efficiency of four different breeding methods in tobacco varietal development. A single Fl hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L Bright Yellow 4(BY4) and NC95, was selfed. F2population above cross was screened for resistance against bacterial wilt caused by Pseudommonas solanacearum E.F.Smith under the naturally infested field conditions, and the 30 lines were developed from F2 individual plant by anther culture (ADH), maternal method utilizing Nicotiana afpicana (MDH), single- seed descent(SSD) and Bulk breeding method, respectively. All characters except content of total alkaloids of ADH and MDH which wore bred by haploid methods reduced more than that of lines bred by conventional methods(SSD & Bulk) : however, the yields were 8% lower than other lines. The total alkaloid content of ADH was higher than that of MDH, and yield was reduced about 4n even though the number of leaf was identical with the MDH. All other characters of ADH were also reduced significantly. In the lines bred by conventional methods, population developed by SSD showed significantly wider leaf width, shorter plant height, later days to flower, and lower in percent reducing sugar than those by Bulk. The populations derived from haploid method showed greater phenotypic variance and wider range of variation than conventionally developed ones. The results obtained indicate that selection will provide a significantly greater genetic gain for leaf number and leaf length in the ADU and MDH populations, and for plant height and days to flower in the SSD and Bulk populations.

황색종 연초의 약배양 및 종간교배에 의한 반수체 배가계통의 특성비교

정윤화,이승철,김달웅,Jeong, Yun-Hwa,Lee, S. C.,Kim, D. U. 한국연초학회 1992 한국연초학회지 Vol.14 No.1

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single F t hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC 95, was used to develop the 30 dihaploid lines by anther culture(F1-ADH) and maternally-derived doubled haploid utilizing Nicotiana africana(F1-MDH), respectively. As compared with mid-parent, ADH lines showed increasing in number of leaves, delaying in days to flower and narrowing in leaf width. However, no significant differences in the other characters investigated were recognized. MDH lines also showed narrow leaf width, while no significant differences in the other characters were observed. The variations of the characters investigated were generally greater in ADH than MDH lines. MDH lines had higher plant height and shorter days to flower than ADH lines, while other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were plant height, leaf number, days to flower, and yield, while those showing relatively low value were leaf length, leaf width, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(F1-ADH, F1-MDH) and correlated responses associated with selection for yield were estimated. Plant height, leaf width, days to flower, percent reducing sugar and disease resistance would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

연초 (Nicotiana tabacum L.) 감자바이러스Y 저항성 품종육성 I. 황색종 품종 McNair30의 감자바이러스Y 저항성유전

정윤화,정석훈,금완수,최상주,이승철 한국연초학회 1984 한국연초학회지 Vol.6 No.2

To classify the inheritance of resistance to potato virus Y, crosses between susceptible flue-cured tobacco variety NC 95 and resistant variety McNair 30 were conducted. The parents, $F_1$ plants, $F_2$ populations, and haploid plants derived from anthers of $F_1$ plants were screened for a resistance of two potato virus Y strains (PVY-VB and PVY-VN) isolated in Korea. The Chi-square values for the $F_3$ populations and haploids of $F_1$ fitted 1 :3 and 1 :1 ratios of resistant to susceptible for two strains, respectively. Therefore, it was found that the resistance of McNair 30 for the potato virus Y was controlled by a single recessive gene. Moreover the resistance to two strains screened was inherited dependently.

Development of Tobacco Mosaic Virus (TMV) resistant Flue-cured F1 Hybrid, KF118

정윤화,조수헌,정석훈,Wan-SooKeum,Sang-JuChoi,Suk-HunJung,Yue-GyuKang,Youl-YoungChung 한국작물학회 2003 Korean journal of crop science Vol.48 No.5

Tobacco mosaic virus (TMV), TMV-common strain, causes severe damage at growth, yield and leaf quality on flue-cured tobacco in Korea. Controlling TMV outbreak with cultivating practices and chemicals are limited, thus, the best way would be developing TMV resistant variety. A new flue-cured hybrid, KF118, was developed from a cross of maternal parent MSTC175 and TC853 at the Agro-tech. Research Group, KT&G Central Research Institute. KF118 exhibited more vigorous growth characteristics in early stage than that of NC82, standard variety in Korea, while other characters were favorable to those of NC82, It is highly resistant to TMV and bacterial wilt(Ral-stonia solanacerum), and black shank(phytophthora parasitica var. nicotianae) is comparable to that of NC82. It should adapt well to the flue-cured production area, and can reduce premature flowering under unfavorable weather conditions. Yield of KF118 is 1 to 2% higher, and leaf quality is 4% higher than NC82. Fl hybrid, KF118, met acceptable standards for chemical and physical characteristics of cured leaf, and ranked high in good smoking taste by panel members in KT&G.

18F-fluoro-2-deoxy-D-glucose positron emission tomography findings of neurolymphomatosis

정윤화,우인숙,한덕재,한치화 대한혈액학회 2014 Blood Research Vol.49 No.2

Extraction and Electrophoretic Characterization of Rice Proteins

정윤화,김미숙 한국식품영양과학회 2002 Preventive Nutrition and Food Science Vol.7 No.4

Rice proteins were extracted from brown and milled rice of five varieties: Kwanganbyeo, Daeanbyeo, Daejinbyeo, Surabyeo, Hwaseongbyeo; and their electrophoretic patterns were analyzed by SDS-PAGE. Albumin was extracted with water, globulin with 5% NaCl, prolamin with 70% ethanol, and glutelin with 0.2 M sodium borate buffer (pH 10.0) containing 0.5% SDS, 0.6% -mercaptoethanol. The ratios of albumin : globulin : prolamin : glutelin in the brown rice were 10.8∼14.1 : 12.4∼16.4 : 3.6∼5.3 : 68.6∼72.8, and in milled rice were 4.4∼5.6 : 10.6∼12.0 : 3.9∼5.4 : 75.7∼79.8. In albumin seven major bands were observed with molecular weights ranging from 14.9∼96.8 kDa, in globulin four bands with molecular weights in the range of 14.4∼56.9 kDa, prolamin had only one band with a molecular weight of 14.4 kDa, and glutelin had four bands with molecular weights of 14.4∼57.4 kDa. There were no differences in electrophoretic patterns between rice varieties or between brown and milled rice.

『묻고, 묻지 못한 이야기 : 담벼락에 묻힌 5월 광주』 문선희 엮음, 난다, 2016

정윤화,Jeong, Yun-Hwa 한국기록학회 2016 기록학연구 Vol.0 No.49

황색종 연초품종의 자연교잡율

정윤화,금완수,조명조,이승철 한국작물학회 1993 Korean journal of crop science Vol.38 No.6

연초 종자생산의 기초자료를 얻기 위하여 임성품종과 웅성불임 품종을 공시하여 격리거리 및 이식시기에 따른 자연교잡율을 조사하였다. 1. 임성품종간의 인접재배시 자연교잡율은 평균 1.6%이었고 0.9~2.5%범위로 나타났다. 2. 임성품종과 웅성불임 게통의 인접재배시 자연교잡율은 29%로 임성품종에 비하여 현저히 높게 나타났다. 3. 이식시기따른 자연교잡율은 4월20일 이식한 처리구가 5월 5일 및 5월20일 이식한 처리구보다 현저히 높았다. 4. 격리거리에 따른 자연교잡율은 격리거리가 멀수록 낮았고 300m 격리구에서도 자연교잡이 0.8%로 나타났다. This experiment was conducted to investigate the amount of natural crossing at varying isolation distance and various transplanting time in tobacco. Normal variety and male sterile line showed an average of 1. 6 and 29% natural crossing in the adjacent plantings, respectively. The natural crossing was highest in the adjacent planting, as expected, and less at the farther distance from pollinator plants. Plants transplanted in April 20th were higher in natural crossing than those of other transplanting time.