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조갑진균증의 진단 방법으로서 조갑판 PAS 염색 및GMS 염색의 유용성에 대한 연구
전지현,김일환 대한의진균학회 2005 대한의진균학회지 Vol.10 No.1
Background: Tinea unguium is a common problem seen in clinical practice. Considering the many differential diagnoses of dystrophic nails, it is important to make a definitive diagnosis of dermatophyte infection before the initiation of antifungal therapy. Potassium hydroxide (KOH) preparation and fungal culture, which are commonly used in the diagnosis of these infections, often yield false-negative results. Recent reports have suggested that nail plate biopsy (Bx) using periodic acid-Schiff (PAS) (Bx/PAS) stain may be a very sensitive technique for the diagnosis of tinea unguium. Objective: The purpose of this study was to evaluate the usefulness of PAS and Grocott's methenamine silver (GMS) staining of nail specimen in the diagnosis of tinea unguium as a standard method. Methods: We evaluated 75 nail specimens from suspected tinea unguium using KOH preparation, biopsy using periodic acid-Schiff stain, and Grocott's methenamine silver stain. Results: Of the 75 nails which were negative on potassium hydroxide mounting, 43 and 39 cases were tested positive respectively on periodic acid-Schiff stain and Grocott's methenamine silver stain. Conclusion: Bx/PAS and Bx/GMS are the sensitive methods for the diagnosis of tinea unguium. They are indicated if clinical suspicion of onychomycosis is high and KOH preparation shows no fungal elements.
기립성 단백뇨와 특발성 만성 피로를 동반한 Nutcracker 증후군 1례
전지현,유병원,이재승,김명준,Juhn Ji Hyun,Yoo Byung Won,Lee Jae Seung,Kim Myung Jun 대한소아신장학회 2001 Childhood kidney diseases Vol.5 No.1
Nutcracker증후군은 좌측 신정맥이 대동맥과 상장간막 동맥에 압박되어 발생하는 드문 질환으로 혈뇨, 단백뇨, 기립성 단백뇨의 원인이 된다. 초음파와 도플러 검사로 진단이 가능하다. 저자들은 기립성 단백뇨와 특발성 만성피로를 보인 보기드문 심한 Nutcracker 증후군 환아를 경험하였기에 문헌고찰과 함께 보고하는 바이다. The nutcracker syndrome is the congestion of left renal vein due to the compression of left renal vein by the aorta and the superior mesenteric artery and has been known as tile cause of hematuria with or without left renal flank pain, mild to moderate proteinuria and orthostatic proteinuria. We present here one case of 13.5 year of girl has severe typical nutcracker syndrome with orthostatic protinuria and idiopathic chronic fatigue. (J. Korean Soc Pediatr Nephrol 5 . 64- 8, 2001)
RE-11 : Metformin modulates the GnRH - induced ERK activation in LβT2 gonadotropes
전지현,박소연,정경아,정혜원,이사라 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: Metformin has been used as a new treatment option in PCOS women. Most recently, the spectrum of metformin`s targets has been expanded, and molecular studies have explored the tissue-specific mechanisms of metformin in the liver, the muscle, the endothelium, and the ovary. Among various mechanisms that reported to be related with Metformin`s action in PCOS, we want to evaluate the effect on reactive oxygen species (ROS) in the GnRH induced ERK activation. 방법: The primary gonadotrope cell line, LβT2 was maintained in DMEM supplemented with glulcose, 10% fetal bovine serum, and 5% penicillin/ streptomycin and incubated in a humidified atmosphere of 5% carbon dioxide. Cells were treated for 5 min of GnRH and Metformin (50 and 500ng/ml) for 1hr, 6hr, and 24hr. Western blotting was performed with 1:1,000 dilution of anti phospho-ERK primary antibody and anti-ERK rabbit primary antibody to control. Immunofluoresence was used and quantification of total ERK phosphorylation was performed relative to total ERK in the same lane. 결과: ERK phosphorylation by GnRH was significantly reduced with Metformin (50 ng/ml) for 24hr treatment (p<0.05) and Metformin (500ng/ml) for 1, 6, and 24hr treatment compared with control. (p<0.05) 결론: Metformin decreased the GnRH induced ERK activation in LβT2 gonadotropes in time dependent manner.
X-linked Myotubular Myopathy in a Family with Two Infant Siblings: A Case with MTM1 Mutation
전지현,이철,남궁란,박민수,박국인,이진성,김세훈 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.3
X-linked myotubular myopathy (XLMTM) is a rare congenital muscle disorder, caused by mutations in the MTM1 gene. Affected male infants present severe hypotonia,and generalized muscle weakness, and the disorder is most often complicated by respiratory failure. Herein, we describe a family with 2 infants with XLMTM which was diagnosed by gene analysis and muscle biopsy. In both cases, histological findings of muscle showed severely hypoplastic muscle fibers with centrally placed nuclei. From the family gene analysis, the Arg486STOP mutation in the MTM1 gene was confirmed.