Streptomyces sp. AO-0511이 생산하는 Herbimycin A 및 Dihydroherbimycin A의 이화학적 특성 및 생물 활성

장흥배,김세찬,김재헌,Chang, Hung-Bae,Kim, Se-Chan,Kim, Jae-Heon 한국미생물학회 2006 미생물학회지 Vol.42 No.1

A streptomycete strain was isolated from the soil samples from Korea. The chemotaxonomy and 16S rDNA sequencing confirmed that the strain belonged to the genus Streptomyces and we named it Streptomyces sp. AO-0511. Two antibiotics, herbimycin A and dihydroherbimycin A produced by this strain were tested for their physico-chemical and biological characteristics. Both compounds were stable under acidic pH. Dihydroherbimycin A was more heat-stable and polar compared with herbimycin A. Only weak antibacterial activities were detected against Bacillus subtilus ATCC 6633 and Micrococcus luteus ATCC 9341. However, herbimycin A and dihydroherbimycin A showed strong inhibitory activities on lung cancer cells (A549 cells) and leukemia cells (HL-60). The cytotoxicity was determined using L5178Y and P388 cell lines. The results showed that herbimycin A and dihydroherbimycin A had lower toxic effects on the cells compared with the standard compounds, comptothecin and cyclosporin A. Therefore, both compounds could be good candidates for the development of new anticancer drugs.

Streptomyces sp. ᄂ79-290 배양액 유래의 산성지 용성 항균활성 물질의 분리정 제

장흥배(Hung Bae Chang) 산업기술교육훈련학회 2014 산업기술연구논문지 (JITR) Vol.19 No.3

In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L79-290 was discovered which produced a water-nonsoluble acidic antibiotic L79-290. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L79-290 was tested by several carbon and nitrogen sources such as maltose and peptone, respectively. L79-290 showed anti-microbial activities against several Gram-positive and Gram-negative bacteria. From the culture supernatant, L79-290 was purified by silicagel column chromatography follow after solvent and water extraction. In the pH stability test, L79-290 showed good stability over 80% under acidic and alkali conditions, respectively. Based on the spectroscopic measurement including ultraviolet(UV), infrared(IR), and fast atomic bombardment mass(FAB-Mass) spectrom analyses, L79-290 was identified as novobiocin. Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic that is produced by Streptomyces niveus.

Streptomyces sp. L79-333 배양액 유래의 항균활성물질의 분리정제

장흥배(Hung Bae Chang) 산업기술교육훈련학회 2016 산업기술연구논문지 (JITR) Vol.21 No.3

The strain designated as Streptomyces sp. L79-333 produced a water-insoluble compound, named as KP-2, which showed anti-microbial activities against several Gram-positive and negative bacteria under paper disk anti-microbial assay. The production of KP-2 in Streptomyces sp. culture broth was exarnined using several carbon and nitrogeo sources such as maltose and skim milk for their effects on growth of Streptomyces sp. as well on antibiotic activity. KP-2 was purified from the culture supernatant by 2-step silica gel column chromatography followed by solvent extraction. KP-2 represented a high pH stability under acidic and alkaline conditions showing more than 80%. Based on the studies of its spectroscopic properties using ultraviolet(UV) and infrared(IR), and fast atomic bombardment mass(FAB-Mass) spectrum analyses, KP-2 was identified as echinomycin. Echinomycin, also known as quinomycin A, is ao quinoxaline group antibiotic.

Streptomyces sp. L77-140 이 생산하는 항생물질의 분리정제 및 동정

장흥배(Hung-Bae Chang) 산업기술교육훈련학회 2011 산업기술연구논문지 (JITR) Vol.16 No.4

In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L77-140 was discovered which produced a water-soluble basic antibiotic L77-140. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L77-140 was tested by several carbon and nitrogen sources such as soluble starch and soybean flour, respectively. L77-140 showed anti-microbial activities against Gram-positive and Gram-negative bacteria. From the culture supernatant, L77-140 was purified by cation exchange chromatography and silicagel column chromatography. In the pH stability test, L77-140 was stable under acidic and alkali conditions. Based on the spectroscopic measurement including Ultra Violet(UV), In Frared(IR), Nuclear Magnetic Resonance (NMR) and mass spectrum(Mass) analyses, L77-140 was identified as spenolimycin.

당귀,지황,홍삼 첨가에 따른 발효 청국장의 기능성 변화 연구

최은지 ( Eun Ji Choi ),이정숙 ( Jung Sook Lee ),장흥배 ( Hung Bae Chang ),이미숙 ( Mee Sook Lee ),장해동 ( Hae Dong Jang ),권영인 ( Young In Kwon ) 한국미생물생명공학회 2010 한국미생물·생명공학회지 Vol.38 No.4

Cheonggukjang is one of the traditional fermented soy-based foods in Korean diets. Studies in cell cultures, humans have revealed anti-hypertension, anti-stress, anticancer, antioxidant, immune enhancing effects. Angelica gigas, Rehmanniae radix, and Red ginseng are popular medicinal plants and widely used for oriental medicine. In this study a strategy had been developed to mobilize beneficial phenolics from Angelica gigas, Rehmanniae radix, and Red ginseng combined with fermented soy by Cheonggukjang fermentation for antioxidant and Type II diabetes management. The quality and functional characteristics of Chenggukjang fermented with Angelica gigas, Rehmanniae radix and Red ginseng. Cheonggukjang (CKJ), Angelica gigas Cheonggukjang (CKJ-DD), Rehmanniae radix Cheonggukjang (CKJ-RG), Angelica gigas and Rehmanniae radix Cheonggukjang (CKJ-DD+RG) and Red ginseng Cheonggukjang (CKJ-RED) were evaluated. The mobilized phenolic profile was evaluated for antioxidant activity and the potential to inhibit α-amylase linked to hyperglycaemia. This research has important implications for the development of functional soy-based-fermented foods enriched with Angelica gigas, Rehmanniae radix and Red ginseng phenolics for oxidative stress - induced diabetic complications. Furthermore, Hunter`s color values of 5 types cheonggukjang, lightness (L-values), redness (a-values) and yellowness (b-values) were evaluated. Free amino acid content of CKJ-RED (0.993 mg/gd. w.) showed higher than that of CKJ (0.205 mg/g-d.w.).

친화성 고분자 유도체의 합성 및 단백질의 분리정제에 관한 연구 - Benzoyl - AH - Sepharose 4B 의 합성 및 흰느타리버섯중 단백질의 분리정제 -

민태진,장흥배,최원기 ( Tae Jin Min,Hung Bae Chang,Won Ki Choi ) 한국균학회 1988 韓國菌學會誌 Vol.16 No.3

Synthesis of Resin Derivatives and Purification of Protein : Synthesis of Benzoyl-AH-Sepharose 4B and Purification of Protein in Pleurotus cornucopiae(Per.)Rolland Benzoyl-AH-Sepharose 4B의 합성 및 흰느타리버섯 중 단백질의 분리정제

Min,Tae-Jin,Chang,Hung-Bae 동국대학교 자연과학연구소 1988 자연과학연구 논문집 Vol.8 No.-

흰느타리버섯중의 단백질을 분리정체하기 위해서 AH-Sepharose 4B 겔을 출발물질로 하여 benzoyl-AH-Sepharose 4B를 합성한 다음 친화성 크로마토그래피 하였다. 합성겔의 ligand 인 benzoyl기의 capacity는 겔 1ml당 9.3 micromole이었다. 합성겔인 benzoyl-AH-Sepharose 4B에 친화성이 있는 단백질과 비친화성 단백질들의 겉보기 분자량은 각각 29.5, 31.5, 34.0, 71.0 및 89.0KD와 1.6, 4.6, 7.0, 35.0 및 61.0KD였으며 비극성, 극성, 양성 및 음성전기를 갖고있는 아미노산의 조성은 각각 45.68, 26.93, 11.81 및 15.58%와 42.56, 29.56, 12.90 및 14.98% 였다. 비교실험하기 위해 실시한 AH-Sepharose 4B겔에 의한 친화성 크로마토그래피에서 겔에 친화성이 있는 단백질들의 겉보기 분자량은 각각 3.2, 31.0 및 61.0KD였고 , 비극성, 극성, 양성 및 음성전기를 갖는 아미노산의 조성은 각각 44.05, 29.13, 13.91 및 12.91%였다. 합성겔의 hydrophobic한 ligand인 benzoyl기에 의해서 비극성 단백질들이 선택적으로 분리되었다. For selective purification of protein in Pleurotus cornucopiae(Per.) Rolland, affinity chromatography was performed by benzoyl-AH-Sepharose 4B gel synthesized using AH-Sepharose 4B with starting material. Ligand capacity of benzoyl group was 9.3micromole per milliliter of gel. Apparent molecular weights of affinity proteins eluted from benzoyl-AH-Sepharose 4B were 29.5, 31.5, 34.0, 71.0 and 89.0KD, while that of nonaffinity proteins were 1.6, 4.6, 7.0, 35.0 and 61.0KD respectively, and the contents of nonpolar, polar, positively charged and negatively charged amino acids in affinity proteins were 45.68, 26.93, 11.81 and 15.58%, while that nonaffinity proteins were 42.56, 29.56, 12.90 and 14.98% respectively. Apparent molecular weights of affinity proteins eluted from AH-Sepharose 4B were 3.2, 31.0 and 61.0KD, respectively, and the contents of nonpolar, polar, positively charged and negatively charged amino acids were 44.05, 29.13, 13.91 and 12.91%, respectively. The nnpolar proteins were selectively purified by hydrophobic ligand of benzoyl group of gel.