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Bacillus lentimorbus WJ5의 분비 전용 Srb Homologue 발현과 항진균 활성
장유신,이영근,김재성,조규성,장병일 한국미생물학회 2003 미생물학회지 Vol.39 No.3
Bacillus sp.는 다량의 효소와 기능성 펩타이드들을 세포외로 분비하는 것으로 알려져 있다. Signal recognition particle (SRP)과 SRP receptor는 세포외 분비 단백질의 이동에 있어서 중심적 역할을 담당한다. B. lentimorbus WJ5의 DNA microarray 결과, 감마선 조사로 유도된 항진균 활성 결핍 돌연변이체인 WJ5m12에서 B. subtilis srb homologue (srbL)의 발현이 감소되는 것을 관찰할 수 있었다. SRP receptor와 항진균 활성 사이의 연관성을 고찰하기 위하여 B. lentimorbus WJ5의 srbL을 PCR로 증폭하여 pQE30 vector에 클로닝 하였으며, B. lentimorbus WJ5m12에 형질전환 시켰다. 형질전환된 B. lentimorbus WJ5m12::srbL은 항진균 활성이 복원되었다. 이차원 전기영동 결과, 수 종의 세포외 분비 단백질의 전구체들이 항진균 활성 결핍 돌연변이 균주에서 축적되었고 B.lentimorbus WJ5m12::srbL에서는 야생형 균주의 단백질 수준까지 감소되는 현상을 관찰할 수 있었다. 이는 B.lentimorbus WJ5의 항진균 활성 관련 물질의 이동에 있어서 srbL이 중요한 역할을 한다는 것을 암시한다. Bacillus sp. secretes high levels of extracellular enzymes into the culture medium. The signal recognition particle (SRP) and the SRP receptor play a central role in targeting pre secretory proteins to the translocase. By the analysis of the DNA microarray of B. lentimorbus WJ5, it was detected that WJ5m12, antifungal activity deficient mutant induced by gamma radiation, had a down-regulated expression of the SRP receptor gene (B. subtitis srb homologue, srbL). To determine the relationship of SRP receptor to antifungal activity, srbL of B. lentimorbus WJ5 was amplified by PCR and ligated into pQE30 vector, and then transferred into WJ5m12. The transformant, WJ5m12::srbL, recovered the antifungal activity. From the 2-DE analysis, the several presecretory proteins accumulated in the mutant cell and decreased to a level of the wild type in WJ5m12::srbL. It seems that the srbL could play an important role in the secretion of the antifungal activity related proteins of B. lentimorbus WJ5.
장유신,박수진 한국공업화학회 2002 한국공업화학회 연구논문 초록집 Vol.2002 No.0
Activated carbon fibers (ACFs) are one of the best supports so far, metal or metal oxides supported on ACFs can be used as the active catalyst phases. In this work, the catalytic reduction of NO over silver supported ACFs prepared by electrolytic silver plating has been studied. It is found that the silver content on carbon surfaces increases with increasing the plating time. However, a slightly gradual decrease of adsorption properties, including specific surface areas and total micropore volumes based on t-plot approaches, in increasing the plating time is observed within the range of well-developed micropore structures. As an experimental result, the net heat of adsorption or BET's ‘C’ of the silver supported ACF catalysts is largely influenced on the amount of silver metal. Consequently, the catalytic ability for NO removal over silver supported ACF catalyst is resulted in the silver content, and in the surface and adsorption properties of the samples, as well as, the reaction time increases, for the catalytic reaction of NO-Ag and NO-C.
장유신,박수진 한국공업화학회 2002 한국공업화학회 연구논문 초록집 Vol.2002 No.0
A widely used method for activated carbon fibers (ACFs)/Cu catalyst is nitric oxide reduction, which includes the process involved whenever nitric oxide (adsorptive) is brought into contact with a solid (adsorbent), and then reduced into nitrogen and oxygen. In this work, the catalytic reduction mechanisms of NO over ACFs/Cu prepared by electrolytic copper plating has been studied. It is found that copper content on carbon surfaces increases with increasing the plating time. However, a slightly gradual decrease of adsorption properties, such as, BET specific surface area, in increasing the plating times is observed within the range of well-developed micropore structures. As an experimental results, nitric oxide is converted into the nitrogen and oxygen on ACFs and ACFs/Cu catalyst surfaces at 500°C. Especially, ACF/Cu catalyst surfaces scavenge oxygen from catalytic reaction. K is indicated that there are different catalytic reduction mechanisms of nitric oxide over ACFs and ACF/Cu catalyst.