체형에 따른 성인 남녀의 생활습관에 관한 연구

이희섭 한국조리과학회 1997 한국식품조리과학회지 Vol.13 No.2

성인 남녀를 비만도에 따라 저체중군의 체형, 표준체중군의 체형, 과체중군의 체형으로 구분하여 체형에 대한 인식, 기호습관, 식습관, 건강에 대한 관심도, 체중 조절에 대한 태도 등을 조사한 결과는 다음과 같다. 1. 조사대상자 중 저체중군의 체형이 7.9%, 표준체중의 체형이 55.2%, 과체중군의 체형이 36.9%로 나타났으며 저체중군은 마르고 키가 크고 과체중군은 뚱뚱하고 키가 작은 경향을 보였다. 또한 자신의 실제 체형과 자신이 이상형이라고 생각하는 체형과는 차이가 있는것으로 나타났으며 이런 경향은 남자보다는 여자가 더욱 심한 것으로 나타났다(p<0.001). 남자의 42.6%, 여자의 21.5%만이 자신의 체형에 대해 만족하는 것으로 나타났다(p<0.01). 여자는 체중이 적을수록 자신의 체형에 대한 만족도가 증가하였으며 체형과 상관없이 모두 마른 체형을 선호하는 것으로 나타났다(p<0.001). 2. 남자의 86.5%, 여자의 49.0%가 술을 마시는 것으로 나타났으며(p<0.001)저체중군 남자와 표준체중군 여자가 술을 가장 많이 마시는것으로 조사되었다. 음주횟수는 1주일에 한 두 번 마신다가 가장 많았다. 남자의 64%가 담배를 피우는 것으로 조사되었으며 여자들은 대부분 흡연하지 않는 것으로 조사되었다(p<0.001). 기호음료 중에서 남녀 모든 군에서 커피의 선호도가 가장 높게 나타났으며(p<0.01) 남자의 51.0%, 여자의 45.6%가 건강(보조)식품이나 영양제를 먹는다고 응답하였다. 3. 남자의 57%, 여자의 45%가 식사를 항상 규칙적으로 하는 것으로 나타났으며 과체중군 여자를 제외하고 모든 체형에서 식사를 항상 규칙적으로 하는 비율이 높게 나타났다. 전혀 규칙적이 아니라고 답한 비율은 표준체중군 여자에게서 가장 높게 나타났다. 3끼의 식사량에 있어서는 남녀 모두 적당하게 먹는다고 응답한 비율이 가장 높았으며 배부르게 먹는 경우는 과체중군에서 높게 나타났다(p<0.001). 음식의 간에 있어서 남녀 모든 군에서 적당하게 먹거나 싱겁게 먹는 다고 답한 비율이 대부분을 차지하였다. 남자의 78.7%, 여자의 92.6%가 간식을 먹는 것으로 조사되었으며(p<0.01), 저체중군의 간식율이 가장 높게 나타났다. 편식을 안 한다고 답한 비율은 남자가 여자보다 높게 나타났으며(p<0.05), 저체중군의 편식율이 가장 높게 나타났다. 한편, 식사의 질에 대하여 식사를 고칠 필요가 있다고 답한 비율은 남자가 40.4%, 여자가 56.3%로 나타났으며(p<0.01), 다른 군에 비하여 저체중군 남자와 과체중군 여자에게서 높게 나타났다. 4. 수면 시간은 남자는 평균 6.92시간, 여자는 6.53시간으로 조사되었으며, 과체중군 남자가 가장 길었으며, 저체중군 여자가 가장 짧은 것으로 조사되었다. 수면시간에 관해서 남녀 모두 적당하다는 비율이 가장 높았다. 한편 하루 중 노동, 운동, 휴식, 식사의 균형에 관해서 남녀 모두 가끔 그렇다고 응답한 비율이 높게 나타났으며 전혀 아니라고 응답한 비율도 모든 체형에서 24% 이상으로 나타났다. 남자의 70.2%, 여자의 52.3%는 운동을 하고 있는 것으로 조사되었으며(p<0.001)남자는 체형과 상관없이 운동을 가끔하는 비율이 가장 높았으며 전혀 운동을 하지 않는 비율도 24%이상으로 나타났다. 여자의 경우 운동을 항상 하고 있는 비율은 저체중군에서, 가끔하는 비율은 과체중군에서 높게 나타났다. 자각적 건강 상태에 관하여 남녀 모두 과반수 이상이 그저 그렇다고 응답하였으며 건강하여 문제가 없다는 남자에게서, 항상 피곤하고 건강 장애가 있다는 여자에게서 높게나타났다(p<0.05). 항상 피곤하고 건강 장애가 있다고 응답한 경우는 과체중군 남자와 표준체중군 여자에게서 가장 높게 나타났다. 5. 남자의 53.9%, 여자의 83.2%가 체중 조절에 관심이 있다고 하였으며(p<0.001) 남녀 모두 체중이 증가할수록 체중 조절에 대한 관심도가 높아졌다(p<0.01). 체중 조절에 대한 시도 경험에 대해서는 남자는 생각 없다가, 여자는 과거에 몇 차례 해 본 경험이 있다가 가장 높게 나타났다(p<0.001). 현재도 체중조절을 하고 있다는 응답자는 10% 정도로 조사되었다. 체중 조절 시도 방법에 있어서 남자의 저체중군은 체중을 늘이는 방향으로 과체중군은 체중을 줄이는 방향으로 시도하였으며, 여자의 경우에는 모두 체중을 줄이는 방향으로 시도하는 비율이 높게 나타났다(p<0.001). 체중조절을 위한 좋은 방법으로는 남자의 경우 음식량의 조절보다는 운동을 많이 한다가 가장 많았으며 여자들은 음식물의 섭취량을 줄인다와 음식량의 조절보다는 운동을 많이 한다고 답한 비율이 비슷하게 나타났다(p<0.001). The purpose of this study was to investigate total life habits of male and female adult through questionaire. Questionaire was composed of items such as perception of body shape, food habits, preference habits, the concern of the health and weight control. For this study, the subjects were divided into three groups like lowweight group, standard-weight group, over-weight group according to their body shape. The results can be summarized as follows: 7.9% of the subjecets were included in low-weight group, 55.2% in standard-weight group, 36.9% in over-weight group. The subjects perception of ideal body shape was significantly different from that of actual body shape. Males were more satisfied with their body shape than females. Most of females were unsatisfied with present their body shape and prefered slim and long body shape. Males, specially low-weight group, had adherence to smoking, alcohol and health food compared with the other groups. Food habits of males were considered to be fair compared with those of females. Meal amounts of over-weight group were significantly higher than those of the others. Low-weight group took more snacks and took a stong dislike of food. Males exercised hard and thought their health status to be fair. Low-weight group of males and standard-weight group of females considered their health status to be bad. 69% of the subjects were concerned about weight control. Low-weight group of males had a desire to gain weight while most of females had a desire to lose weight.

방사선 조사에 의한 참치 자숙액 에탄올 추출물의 조지방 함량 및 지방산조성 변화 연구 /

이희섭,최종일,김현주,유천우,김주봉,황영정,정영진,김진규,변명우,이주운 한국방사선산업학회 2008 방사선산업학회지 Vol.2 No.2

Antiobesity Effect of Garlic Extract Fermented by Lactobacillus plantarum BL2 in Diet-Induced Obese Mice

이희섭,임원철,이성진,이승현,이진협,조홍연 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.9

Obesity is viewed as a serious public health problem. This study aimed to investigate the antiobesity effects of fermented garlic extract by lactic acid bacteria (LAFGE) on obesity. Male C57BL/6J mice were fed with high-fat diet (HFD) to induce obesity. TheHFD-induced obesemicewere orally administrated with 250 or 500mg/kg LAFGE for 8 weeks. Feeding HFD-fed mice with 250 or 500mg/kg LAFGE reduced body weight by 14% and 18%, respectively, compared to HFD. HFD-fed mice with 500mg/kg LAFGE administration had lower epididymal, retroperitoneal, and mesenteric adipose tissue mass by 36%, 44%, and 63%, respectively, compared to HFD.The concentration of plasma triacylglyceride and total cholesterolwas significantly lower in theHFD-fed mice withLAFGE administration.Moreover,LAFGEsupplementation suppressed adipogenesis by downregulation in mRNA and protein expression of PPARγ, C/EBPα, and lipogenic proteins, including SREBP-1c, FAS, and SCD-1. Based on these findings, LAFGE may ameliorate diet-induced obesity by inhibiting adipose tissue hypertrophy by suppressing adipogenesis.

생쥐의 卵子自然成熟 및 體外受精에 있어서 卵丘細胞의 役割

이희섭,홍기연,이희민,최은하,김원신 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

This study was to determine whether cumulus cell augments spontaneous maturation in vitro of oocyte and in vitro fertilization of the mouse. The maturation rate was evaluated by analyzing the metaphase Ⅱ chromosomal configurations 24 hour after incubation and the fertilization frequency was evaluated by observation of zygotic division to 2-cell 16 hour after insemination. The maturation rate of cumulus-oocyte complex was not significantly different in that of denuded oocytes(86% versus 81%). The fertilization frequency of cumulus-oocyte complex was significantly higher than that of denuded oocytes (59% versus 29%, p<0.05). It is concluded that the presence of cumulus cell does not augment the spontaneous maturation of immature oocyte but augments the fertilization frequency in mouse.

식물성 질소원의 보충이 면양에 의한 볏짚의 소화율에 미치는 영향

이희섭,강희신 ( Hee Seob Lee,Hi Shin Kang ) 한국축산학회 1980 한국축산학회지 Vol.22 No.2

Three Corridale male sheep were fed each of the three diets; Acacia leaf hay alone (AL), chopped rice st raw plus near isonitrogeneous quantity of Soybean oil meal (RS) or Acacia Leaf Hay(RA) according to a Latin square design to studs the effect of supplemental nitrogen on the digestibilities of nutrients and the voluntary intake of rice straw. The results obtained from the above trial were summarized as follows: (1) The voluntary feed intake of acacia leaf hay was found to he 935g/day and its digestibility of dry matter, crude protein and crude fibre were 51.8, 46.0 and 52.0% respectively. (2) Although the total UM intake were not affected by the supplemental nitrogen sources, the intake of chopped rice straw by the sheep at the RS was 11.7% higher thar that of RA. (3) The crude protein intake of sheep at the AL was higher (p. 05) than those of RS and RA. The crude protein digestibility of RS, however, was higher than those of AL and RA as much as 71.7% and 64.2%. (4) Despite the trend that the liber intake of sheep fed chopped rice straw, RS and RA, were higher than that of AL as much as 49.3% and 64.3% the digestibility of crude fiber showed no significant difference among the treatments. The difference between the RS and AL., however, was significant at level of 10% probability. The total digestible nutrients of chopped rice straw was calculated to be 50% in DM basis by employing the TDN value of known Soybean oil meal and Acacia leaf hay.

춘천지역 60세 이상 노년층의 건강식품 섭취실태 조사

이희섭,이혜숙,이정애,강금지 대한지역사회영양학회 2001 대한지역사회영양학회지 Vol.6 No.4

This study was carried out to investigate the intake patterns of health food according to the kind of disease of the elderly aged over 60 years living in the Chuncheon area. This study was conducted by using a questionnaire from December 1999 to the March 2000. The data was analyzed using a SPSS program. The results were summarized as follows : The poorer the self- perceived health condition, the lower the level of activity of daily living(ADL) and instrumental activities of daily living(IADL) were the higher. the frequency score of health food intake. The incidence of disease, such as arthritis(44.7%), hypertension(18.8%), digestive diseases(17.1%), diabetes(10.5%) were found to be higher. than other chronic diseases among the elderly. The frequency score of health food intake was not significantly different according to self-perceived health status, disease status, or the level of ADL and IADl.. The must important source of information for health food intake was the recommendation of friends and relatives(68.7%). Twenty eight point two percent of the elderly considered health foods to be effective, however 8.3% of the elderly considered them to be ineffective. The elderly with chronic diseases had taken health floods in the descending order of neuralgia-arthritis, hypertension, digestive disease, diabetes. The elderly with digestive diseases, diabetes, renal disease, neuralgia- arthritis and respiratory disease were inclined to habitually take health floods once or twice per year. Intake of Korean traditional folk food was as high as intake of registered health food and Chinese tonic medicine. The most frequently taken health food was tonic medicine, which is fo11owed by pumpkin, ginseng products, herbal tea formula with black goat, deer antler and Kye-so-ju. Intakes of health food such as tonic medicine, blood of the deer, royal jelly, aloe were found to be higher among the elderly with chronic diseases such as diabetes, hypertension, and digestive disease. However, intakes of ginseng products, deer antler, pumpkin, herbal tea formula with black goat and Kye-so-ju were found to be higher among the healthy elderly. The elderly with neuralgia-arthritis had taken sixteen kinds of health foods. From these results, a wide consumer education program should be developed to convince people of the importance of well-balanced diet and to choose proper health foods according to the elderly's health conditions. Also, comprehensive and scientific research into Korean traditional folk foods are needed for the correct use.

산모 말초 혈액으로부터 태아 세포의 분리

이희섭,김정중,김원신 원광대학교 생명공학연구소 1997 생명공학연구소보 Vol.5 No.1

We synthesized rabbit antisera to the enzyme, human type Ⅰ 3-HSD(3-hydroxy-5-ene steroid dehydrogenase, EC 1.1.1.145) that is the key enzyme in the stroidogenesis and membrane-bounding enzyme of trophoblasts, by oligopeptide coupling method. This anti-3-HSD antisera reacts with 43kDa protein in human placental lysate by SDS-PAGE and Western blot, and the syncytiotrophoblasts and cytotrophoblasts from placental villi are stained positively with this antisera by immunohistochemistry (published in Bulletin of Life Science and Biotechnology 1995;3:68). Fetal trophoblast cells were isolated from peripheral blood of pregnant women by the immune-bead method using anti-3-HSD antibody and Dynabeads M-280(Dynal A.S. Oslo Norway). Isolated cells were observed directly by inverted microscope and observed with Pap stain by light microscope. Isolated trophoblast cells were adherent to Dynabeads M-280 and exhibited the typical morphology of syncytiotrophoblast cells. We mentioned previously polymerase chain reaction to the Y-chromosome containing gene, DYZ1, SRY, and AMGL is the easy method in sex-determination(published in Kor J Obstet Gynecol 1997;40:1412). For the purpose of fetal cell identification, fetal cells were isolated from peripheral blood of five women who were pregnant with male fetus ascertained by karyotyping of amnionic cells and ultrasonography. Sex-determination of the isolated fetal cells was performed by PCR method. In all cases(5/5, 100%), maleness was confirmed. According to our results, it was concluded that isolation of these fetall cells by immune beads method could allow noninvasive diagnosis of wide range of inherited disorders.

연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

이동용 로봇의 속도 및 방향제어를 위한 퍼지-신경 제어기 설계 ( The Fuzzy-Neural Controller Design for Speed and Azimuth Control of Mobile Robot )

이희섭,한성현,이진,이만형,권태환 대한기계학회 1994 대한기계학회 춘추학술대회 Vol.- No.-

생쥐 난자의 체외 성숙과 수정에 있어서 난구 세포의 역할에 관한 연구

이희섭,류철희,이기숙,오병찬,조성남,진소자,두재균,김관식,김종덕 의과학연구소 1996 全北醫大論文集 Vol.20 No.2

The time course and conditions encessary for oocyte maturation and the role of cumulus cells on fertilization in vitro were studied in the mouse. nuclear maturation of cultured oocytes was evaluated by analyzing chromosomal configurations. Isolated oocytes from cumulus-oocyte complex were matured spontaneously in vitro, nad 81% of oocytes developed to the metaphase Ⅱafter 24 horus inculbation. Cumulus-oocyte complex matured in vitro, and 865 of those developed to the metaphase Ⅱ agter 24 hours. The fertilization frequency of isolated oocytes in hyaluronidase was similar in both +HCG and -HCG culture meticum(29.2% and 21.7%, respecitvely). However the fretilization frequency of cumulus-oocyte complex was greater than that of isolated oocytes, and the fertilization frequency of cumulus-oocyte complex cultrued in +HCG culture meticum was lower than that cultrued in -HCG culture meticum(36.4% and 58%, respectively). It is concluded that frequency of spontaneous maturation of isolated oocyte was similar to that of cumulus-oocyte complex, and that the presence of cumulus cells during fertilization may affect the oocyte's ferilization.