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이준황,원병연,윤현철 한국바이오칩학회 2007 BioChip Journal Vol.1 No.1
The signal stability from electrochemical immunosensors based on the enzymatic back-filling strategy was investigated. The presence of an analyte, especially antibody, which is bound to the surfaceimmobilized capture molecule, could cause a significant signal loss during subsequent handling procedures and signaling due to its limited affinity /stability. Compared to the typical method of detection, the signal from the back-filling immunoassay is generated differently from the covalently immobilized (‘back-filled’) glucose oxidase (GOX) to the sensing surface, not from the bound analyte that is usually labeled with signaling molecules. Therefore, an enhancement of signal stability would be expected with the back-filling immunoassay. To evaluate the merits of the enzymatic back-filling method, various antibody dissociation conditions from immunosensor surfaces were compared in terms of signal stability and sensitivity. As a model immunosensing reaction, the 2, 4-dinitrophenyl (DNP) group was functionalized to the biorecognition surface on a thin-film gold electrode, and anti-DNP antibody was employed as the target analyte. Cyclic voltammetry was used for immunosensor signal registration and to trace the protein adsorption/dissociation process. The back-filling method was found to be suitable for the analysis of immune reactions without labeled-antibody molecules, and the signal stability was comparable to the affinity sensor employing biotin/avidin couple.
이준,황병환,송혜진,장선형,최수영,Lee, Jun,Hwang, Byung Hwan,Song, Hye-Jin,Jang, Seon Hyeong,Choe, Soo Young 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.5
본 시험은 흑홍삼이 함유된 혼합물의 숙취해소효능을 확인하기 위해 수행하였다. 효소원을 이용한 혼합 재료들 간의 ADH와 ALDH의 활성은 시중에 판매되고 있는 타사 비교군에 비해 현저히 높은 값을 보였다. 그리고 알코올을 투여하여 숙취를 유도한 시험동물의 혈중 에탄올의 양과 아세트알데히드의 양을 측정하여 생체 내 알코올 분해효능을 평가한 결과, 흑홍삼혼합물을 섭취한 군에서 알코올 투여 1시간 후부터 에탄올의 양은 숙취유도군에 비해 월등히 낮았으며, 시간이 지날수록 농도 의존적으로 에탄올의 양이 감소하였다. 주요 숙취 증상을 야기하는 아세트알데히드의 양은 숙취유도군에서 시간이 지날수록 증가한 반면, 흑홍삼혼합물 섭취군은 알코올 섭취 1시간 후부터 증가하였다가 시간이 지날수록 빠르게 감소함을 확인하였다. 또한 흑홍삼혼합물이 알코올분해효소의 활성에 미치는 영향을 알아보기 위해 간 조직을 분리해 ADH와 ALDH의 활성을 측정한 결과, 흑홍삼혼합물을 섭취한 군이 타사 비교군에 비해 높은 활성을 보임을 확인하였다. 이상의 시험결과로 보아 흑홍삼 혼합음료는 혈중 에탄올의 흡수를 지연시킬 뿐 아니라, ADH와 ALDH의 활성을 증가시켜 혈중 에탄올과 아세트알데히드의 농도를 효과적으로 감소시켜 숙취해소 음료로 이용이 가능하리라 기대된다. Ginsenosides are major constituents of ginseng and are known to be responsible for its pharmacological properties. This study aimed to investigate the detoxification effect of a mixture containing black red ginseng powder, red ginseng extract, Puerariae radix extract, and Hovenia dulcis extract, on SD (Sprague Dawley) rats treated with 30% ethanol. Thirty minutes before treatment, the animals were orally administered different concentrations of the mixture or water. Results revealed that the concentration of ethanol in blood serum was significantly decreased in the black red ginseng mixture treated group in a dose-dependent manner, as compared with that of the control group. The blood level of acetaldehyde increased until 1 hr after alcohol administration, but the levels rapidly decreased later. Furthermore, ADH and ALDH activities in the hepatic tissue were also increased in the black red ginseng mixture administered group, than in the control group. These results indicate that the black red ginseng mixture has the ability of decomposing alcohol by increasing the ADH and ALDH activities responsible for alcohol metabolism.
북한강 수계 조류대발생 원인종 남조 Anabaena의 분자계통학적 검토
이준 ( Zhun Li ),한명수 ( Myung Soo Han ),황수옥 ( Su Ok Hwang ),변명섭 ( Myeong Seop Byeon ),황순진 ( Soon Jin Hwang ),김백호 ( Baik Ho Kim ) 한국하천호수학회 2013 생태와 환경 Vol.46 No.2
Between May and August 2012, a massive cyanobacterial bloom with Anabaena has been occurred throughout the North Han River. Sampling was conducted at one station on each lake, L. Uham, L. Cheongpyung, and L. Paldang, where occurred a dense bloom, in 13 July. According to the microscopic examination, the blooms was dominated by one specific filamentous cyanobacterium Anabaena and other phytoplankton. Morphologically, previous literature proven that this Anabaena species is A. crassa (Lemmermann) Komark.-Legn. & Cronberg. However, identification of species in a mixed population is complicated due to limited morphological differences. Therefore, with live sample including trichome, akinete and heterocyst, the sequences of 16S rRNA gene of Anabaena isolates were cloned and analyzed, and three 16S rRNA gene sequences of 1188~1520 bp in length were obtained. It was shown from the homologous analysis results that the obtained 16S rRNA sequences were highly homologous to the relevant sequences of A. crassa in GenBank. The 16S rRNA sequences of 63 species were retrieved from GenBank, and the phylogenetic tree was constructed by using these sequences.
황엄지,남상식,이준설,이형운,양정욱 한국작물학회 2017 한국작물학회지 Vol.62 No.1
To obtain basic data on the effects of differences cultivation periods and methods on growth and development of sweet potato tuber. We investigated growth and development of six major cultivars showing different characteristics, such as dry type(Yulmi, YM; Jinhongmi, JHM), intermediate type(Pungwonmi, PWM; Hogammi, HGM), and purple type(Sinjami, SJM; Danjami, DJM). The average tuber weight of YM and JHM was 4.1 and 8.6 g respectively, 50 days after transplant. At 80 days, the weight of YM and JHM rapidly increased to 25.2 and 26.1 g, respectively. Finally, at 120 days, the weight of YM and JHM increased to 120.7 and 79.4 g, respectively. The average tuber weight of PWM and HGM was 13 and 3.3 g, respectively, 50 days after transplant. The development pattern of PWM showed a consistent increase in tuber weight during the cultivation period, an average weight of 104.2 g at 120 days. However, the tuber development pattern of HGM was different from that of PWM. The average tuber weight of HGM rapidly increased for 100 days after transplant and then showed no increase at 120 days. In case of purple type sweet potato, the average tuber weight of SJM consistently increased during the cultivation period, whereas DJM showed rapid increase in average tuber weight at 80 days after transplant. Korean consumers prefer small, round sweet potato approximately 100g in weight. Based on the results, YM, PWM and SJM are classified as early developing cultivars that can be harvested approximately 120 days after transplant. On the other hand, JHM, HGM, and DJM are classified as late developing cultivars that can be harvested approximately 130 days after transplant.
황재삼 ( Jae Sam Hwang ),박인균 ( In Gyun Park ),윤은영 ( Eun Young Yun ),정덕웅 ( Duck Oung Jung ),윤형주 ( Hyung Joo Yoon ),이상범 ( Sang Beom Lee ),김성렬 ( Seong Ryul Kim ),이준하 ( Joon Ha Lee ),김인우 ( In Woo Kim ),권용남 ( 한국잠사학회 2012 한국잠사곤충학회지 Vol.50 No.2
To find diapause-related genes, we were performed by differential hybridization with three types of [α-32P]dCTP-labeled total cDNA probes synthesized from diapause-prepared, diapause-maintained and diapause-activated stage of Bombus ignitus queen. Nine individual cDNA clones were found to be differentially expressed in diapause-maintained and diapause- activated stage. Among these clones, BIDC9(BIDC ; Bombus ignitus differentially expressed clone) was analyzed through full-length sequencing and expression pattern analysis. This clone was specifically expressed in the thorax organ. The effect of Juvenile hormone analog(JHA) and CO2 treatment was examined. JHA treatment induced the expression of BIDC9 cDNA clone abruptly after 4 day of treatment. CO2 treatment induced also the clone after 2 day of treatment. BIDC9 cDNA was identified as Bombus ignitus diapause gene contained an open reading frame of 1376 bp encoding 255 amino acids.
전신성 홍반성 루푸스 환아에서 병발한 혈구 탐식성 조직구 증식증 1례
황자영,노석만,이진,장필상,김영훈,김진택,이준성,Hwang, Ja Young,No, Suk Man,Lee, Jin,Jang, Pil Sang,Kim, Young Hoon,Kim, Jin Tack,Lee, Joon Sung 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.10
본 저자들은 전신성 홍반성 루푸스와 미만성 증식성 사구체신염으로 진단된 남아의 치료과정 중 발열과 혈구 감소증이 지속된 환아에서 이차성 혈구 탐식성 조직구 증식증을 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Hemophagocytic lymphohistiocytosis is a reactive disorder characterized by a generalized non-malignant histiocytic proliferation with prominent hemophagocytosis by stimulated histiocytes in the bone marrow and reticuloendothelial systems resulting in pancytopenia and liver dysfunction. Several diseases including infection, malignancy and autoimmune disease are known to be causative disorders. This case demonstrated histiocytic hemophagocytosis in the bone marrow, resulting in pancytopenia during treatment of systemic lupus erythematosus and did not show any underlying disease.
이준하,김인우,김미애,윤은영,남성희,안미영,이영보,황재삼 한국잠사학회 2015 International Journal of Industrial Entomology Vol.31 No.1
In a previous report, we identified several candidate antimicrobial peptides through de novo RNA sequencing of the centipede Scolopendra subspinipes mutilans. Here, we identify and characterize one of these peptides, Scolopendrasin I. We identified the centipede antimicrobial peptide Cecropin from the centipede transcriptome using an SVM algorithm, and subsequently analyzed the amino acid sequence for predicted secondary structure using a GOR algorithm. We identified an alpha helical region of Cecropin and named it Scolopendrasin I. We then assessed antimicrobial and hemolytic activity of Scolopendrasin I. Scolopendrasin I showed antimicrobial activity against various microbes, including antibiotic-resistant Gram-negative bacteria, in a radial diffusion assay. Scolopendrasin I had potent antibacterial activity against acne-associated microbes in a colony count assay and showed no hemolytic activity in a hemolysis assay. In addition, we confirmed that Scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, two components of bacterial cell membranes. In conclusion, the results presented here provide evidence that this is an efficient strategy for antimicrobial peptide candidate identification and that Scolopendrasin I has potential for successful antibiotic development