정상 및 원추각막에서 각막 전, 후면의 융기 및 시상면만곡도의 비교분석

이종수,이승욱,이승민,이미현,Jong Soo Lee,Seung Wook Lee,Seung Min Lee,Mi Hyun Lee 대한안과학회 2012 대한안과학회지 Vol.53 No.10

Purpose: To compare changes of anterior and posterior corneal elevation, and sagittal curvature according to the severity of keratoconus and to compare differences between keratoconus and normal cornea. Methods: A total of 81 eyes diagnosed with keratoconus and 20 eyes of normal subjects were evaluated with a Pentacam Scheimpflug camera. The keratoconus eyes were divided into 3 groups according to mean keratometer (K): mild (K ≤ 47.0 diopters (D)), moderate (47.0 to 52.0 D), and severe (52.0 D≥). The following parameters were obtained to evaluate the correlation of keratoconus: corneal thickness, anterior and posterior corneal elevation, and sagittal curvature. Results: Out of 81 keratoconus eyes, 56 eyes were mild, 12 eyes were moderate, and 13 eyes were severe keratoconus. The mean central corneal keratometer, anterior and posterior corneal elevation, and sagittal curvature of the keratoconus eyes were 49.7 D, 22.07 μm, 38.16 μm, 52.76 D and the values increased statistically compared to the normal eyes. Furthermore, the values increased significantly with the severity of keratoconus. ROC curve analysis showed the estimated meaningful value for anterior and posterior corneal elevation and sagittal curvature of keratoconus for diagnosis; there were no diagnostic values for corneal thickness and refractive power. Conclusions: The index of 5.5 μm for mean anterior elevation, 12.5 μm for mean posterior elevation, and 44.5 μm for mean sagittal curvature using the PentacamⓇ are useful to diagnose keratoconus. Variation of anterior and posterior elevation, and sagittal curvature measured by PentacamⓇ are useful in understanding the process of keratoconus. J Korean Ophthalmol Soc 2012;53(10):1432-1437

몇종의 세균과 Saccharomyces cerevisiae에 대한 식품첨가물의 향균 특성

이종수,오준세,김나미,금종화,이석건,Lee, Jong-Soo,Oh, Jun-Sei,Kim, Na-Mi,Keum, Jong-Hwa,Lee, Suk-Kun 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2

현재 식품첨가물로 많이 사용되고 있는 유기산과 안정제 및 색소 등의 각종 세균과 Sacch. cerevisiae 에 대한 향균 특성을 조사하였다. 산도 조절용으로 사용되고 있는 젖산, 사과산, 호박산 및 주석산은 L. acidophilus와 Sacch. cerevisiae에 대하여 향균성이 없었으나 B. subtilis등의 세균에 대하여는 향균성이 있었고 특히 사과산은 P. aeruginosa에 대하여 강한 향균력이 있었다.(최소생육저지농도 : 0.05%). 안정제로서의 알긴산과 펙틴은 B. subtilis, E. coli, P. aeruginosa에 대하여 비교적 강한 향균성을 보였고 L. acidophilus에 대하여는 향균성이 없었다. 황색 색소(홍화엘로우)와 적색 색소(Red powder-N)는 향균성이 없었고 표백제인 $NaHSO_3$의 세균에 대한 최소생육저지농도는 0.05%, Sacch. cerevisiae에 대하여는 0.5%로 향균성이 있었다. In order to survey the safety of some food additives, antimicrobial activity of acidulants, stabilizers, antioxidants, natural coloring materials and bleaching agents against 5 strains of bacteria and Sacch. cerevisiae were investigated by dilution method and minimal inhibitory concentration(MIC) method. Malic acid as acidulants displayed the effective antimicrobial activity in vitro against P. aeruginosa and its MIC is 0.05%. Alginic acid and pectin as stabilizer also displayed strong antimicrobial activity against B. subtilis, E. coli and P. aeruginosa, and tannin(antioxidants) and $NaHSO_3$ displayed antimicrobial activity against all bacteria tested. However gums(Arabia, Xanthan, Gua) and natural coloring materials(Hongwha Yellow, Red powder-N) were not affected to growth of bacteria and Sacch. cerevisiae.

겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향

이종수,김은희,김수진,이지은,김나미,Jong Soo Lee,Eun Hee Kim,Soo Jin Kim,Ji Eun Lee,Na Mi Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. Methods: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe® and Systane® at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. Results: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe® and Systane®. The LDH titers increased after Soothe® exposure, but showed no significant difference after Systane® exposure. Soothe® and Systane® treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane® showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe®. Conclusions: The apoptotic responses of Soothe® and Systane® are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane® may be less severe than Soothe® at higher concentrations and longer exposure times. J Korean Ophthalmol Soc 2010;51(12):1643-1651

익상편조직의 섬유모세포에 미치는 사이클로스포린의 영향

이종수,이승욱,이상준,김나미,Jong Soo Lee,Seung Wook Lee,Sang Jun Lee,Na Mi Kim 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. Methods: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 μg/ml (0.0001%), 1 μg/ml (0.0001%), 10 μg/ml (0.001%), 100 μg/ml (0.01%), or 500 μg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-α and IL-1b. IL-6, IL-8 was performed using ELISA kits. Results: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-α and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). Conclusions: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-α were observed compared with that of the control. J Korean Ophthalmol Soc 2012;53(3):466-472

전통 민속주와 전통 인삼주의 생리기능성

이종수,김재호,이대형,김나미,안병학,Lee, Jong-Soo,Kim, Jae-Ho,Lee, Dae-Hyoung,Kim, Na-Mi,Ahn, Byung-Hak 고려인삼학회 2008 고려인삼연구와 산업 Vol.2 No.2

화침을 이용한 흉요추부 압박골절 치험 1례

이종수,한순현,Lee, Jong-Soo,Han, Soon-Hyun 척추신경추나의학회 2002 대한추나의학회지 Vol.3 No.1

Stable compression fractures are most often caused by trauma as traffic accidents. Stable compression fractures can refer pain to the mid-back, which is characterized by the pain in the thoracolumbar spinous prosess. recently we experienced a 19 years old man who had lumbago originating from Stable compression fractures and condition was improved through burning acupunture. 흉요추 이행부의 안정성 압박골절은 통증의 소실과 후만각 증가 억제에 의한 척추의 안정화이다. 이의 원인을 후방인대의 손상에 의한 손상으로 보고 프로로테라피(Prolotherapy)로 이의 치료를 시행 할 수 있다고 생각되며 한방적인 방법으로 화침을 적용할 수 있었다. 최근 저자는 흉요추 이행부의 안정성 압박골절에 의한 요배통 환자를 발견하고 화침치료를 통해 증세 회복에 도움이 되었기에 치료내용과 경과를 문헌고찰과 함께 보고하는 바이다.

송아지 혈청 농도가 인체의 각막상피세포에 미치는 영향

이종수,김은희,김수진,Jong Soo Lee,MD,PhD,Eun Hee Kim,MD,Su Jin Kim,MD 대한안과학회 2011 대한안과학회지 Vol.52 No.7

Purpose: To investigate the biologic effects of topical calf serum on corneal epithelial cells in vitro. Methods: The effects of calf serum on the corneal epithelial cells were evaluated using the MTT (3-[4,5-Dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide) assay, and the concentration of IL-1α, TGF-β1 and MMP-9 in the cells was measured. Cell damage was determined using lactate dehydrogenase (LDH), and cellular morphologies were examined by transmission electromicroscopy. Results: Metabolic activity of the corneal epithelial cells decreased at higher concentrations and longer exposure durations. IL-1α, TGF-β1 and MMP-9 titers were lower in calf serum-treated cells than in the control. LDH and cellular damage to the corneal epithelial cells, such as chromatin margination and cytoplasmic organelle swelling, were prominent in cells treated with 30% calf serum. Conclusions: Cellular metabolic activity was higher and cellular toxicity was lower in cells treated with 10% calf serum compared to those treated with the 20% and 30% concentrations. Furthermore, inflammatory cytokines were sufficiently inhibited in cells treated with the 10% solution. These results indicate that 10% calf serum could be used clinically. J Korean Ophthalmol Soc 2011;52(7):852-858

β-galactosidase를 생성하는 고온성(高溫性) 세균(細菌)의 생리적(生理的) 특성(特性)에 관한 연구(硏究)

이종수,김찬조,Lee, Jong Soo,Kim, Chan Jo 충남대학교 농업과학연구소 1982 Korean Journal of Agricultural Science Vol.9 No.1

고온균(高溫菌)의 생리적(生理的) 특성(特性)과 내열기구(耐熱機構)에 관한 기초자료(基礎資料)를 얻고자 온천토양(溫泉土壤)에서 ${\beta}$-galactosidase를 생산(生産)하는 고온균(高溫菌)을 분리(分離)하여 동정(同定)하고, 몇가지 주요(主要)한 생리적(生理的) 특성(特性)을 검토(檢討)하였으며, 그의 균체지방산(菌體脂肪酸) 조성(組成)을 gas chromatography로 분석(分析)하여 다음과 같은 결과(結果)를 얻었다. 1. ${\beta}$-galactosidase를 생산(生産)하는 공시(供試) 균주(菌株)는 Thermus속(屬)으로 동정(同定)되었다. 2. 분리선정(分離選定)한 균주(菌株)의 최적(最適) 생육온도(生育溫度)는 $65^{\circ}C$이었고, $37^{\circ}C$에서 생육(生育)하지 않는 절대(絶對) 고온균(高溫菌)이었으며 최적(最適) pH는 6.5내외(內外)이었다. 3. NaCl에 대(對)한 내성(耐性)은 NaCl 1% 이상(以上)에서 생육(生育)하지 못했다. 4. 공시균주(供試菌株)의 항생물질(抗生物質) 내성(耐性)은 Penicillin G는 $10{\mu}g/m{\ell}$, Chloramphenicol은 $0.5{\mu}g/m{\ell}$이었다. 5. Vitamin 요구성(要求性)은 Ca-pantothenate와 pyridoxine-HCl을 절대적(絶對的) 생육인자(生育因子)로 요구(要求)하였고 niacin을 자극적(刺戟的) 생육인자(生育因子)로 요구(要求)하였다. 6. 공시균주(供試菌株)는 최대(最大) 흡수파장(吸收波長)이 420nm 부근(附近)의 색소(色素)를 생성(生成)하였고 그의 생성능(生成能)은 약(弱)하였다. 7. 공시균주(供試菌株)의 균체 지방산(脂肪酸) 조성(組成)은 palmitic acid 60.20%, lauric acid 11.80%, myristic acid 7.56%, behenic acid 4.25%, capric acid 1.77%, stearic acid 2.13%, arachidic acid 1.53%이었다. This experiment was carried out to elucidate the thermotolerant properties of a thermophilic bacterium which was isolated from soils of the hot springs area and selected for the ${\beta}$-galactosidase production. This bacterium was identified as a strain belong to the genus Thermus. Biochemical and physiological characteristics of this strain were studied, including the investigation of the fatty acid composition of its neutral fats. The results obtained were summarized as follows. 1. Optimal temperature and pH for growth of this strain were $65^{\circ}C$ and pH 6.5 respectively, and it was found to be an absolute thermophilic bacterium which could not grow at the temperature below $43^{\circ}C$. 2. No growth was obtained in the medium which contained more than 1.0% of sodium chloride. 3. The tolerable concentration of antibiotics were 10mg of penicillin G per ml of medium and 0.5mg of chloramphenicol per ml respectively. 4. This strain had auxotrophilic requirements for calcium-pantothenate and pyridoxin-HCl as an essential factor and for niacin as a stimulative factor. 5. Yellow pigment was released into the liquid culture of this strain, which showed maximum absorption at 420 nm. 6. Fatty acid composition of neutral fats of the strain was palmitic acid, 60.20%; lauric acid, 11.80%; myristic acid, 7.56%; behenic acid, 4.25%; capric acid, 1.77%; stearic acid, 2.13%; arachidic acid, 1.53%; and others unidentified, 10.7%.

공무원의 전문성 향상방안으로서 경력개발제도(CDP)에 관한 연구 : CDP의 시각에서 분석한 한국 인사행정체계의 문제점과 개선방안

이종수 한국행정연구원 2002 韓國行政硏究 Vol.11 No.4

인사행정의 본질적 과제 가운데 조직의 수요와 개인의 욕구를 결합시키는 역할이 중요성을 더해가고 있다. 이를 위해 최근 각 국 정부는 경력개발제도(CDP)를 주목하고 있으며, 한국의 중앙인사위원회도 2002년 대통령에게 경력개발제도의 도입검토를 보고한 바 있다. 본 연구에서는 최근 정부의 인력관리에 적용가능성이 타진되고 있는 경력개발제도의 개념을 소개하고, 공무원들의 이에 관련된 인식을 분석하며, 나아가 경력개발제도의 적용을 위한 개선과제를 검토하여 보았다. 이를 위해 연구방법으로 문헌연구와 설문조사, 심층면접, 브레인스토밍(Brain Storming)방법을 병행하였다. 분석 결과, 한국의 공무원 인사행정 체계에서 나타나는 계급제적 채용제도, 직위분류제도의 미정착이 경력개발제도의 요소를 필요로 하는 기본배경이 되고있다. 조직문화와 순환보직제도, 짧은 보직기간, 직무수행을 위한 know-how의 취득원과 교육훈련의 불일치 등은 경력개발을 통한 전문성 제고를 저해하는 요소로 분석되었다. 결론적으로, 경력개발제도는 한국 공무원의 전문성 제고와 직무수행 역량의 향상을 위한 다양한 쟁점을 제기하고 있고, 발전방안을 시사하고 있다. The findings suggest a modification to the current uni-dimensional approach to study organizational culture. Public personnel administration has been subjected to virtually uninterrupted wave of reforms under the legacy of New Public Management. It has been the key area of governmental reforms during the past two decades. Many efforts were made to initiate structural changes also in Korea. However. since the feature of traditional class system prevails in the human resource management of the Korean government, it is difficult to achieve the utmost cfficiency. This paper examines major issues of public personnel administration in Korea, particularly form the viewpoint of CDRCareer Development Program). The purpose of this paper is threefold. First, the notion of CDP is introduced to the field of public personnel administration research. Second, the current problems of personnel administration in Korea are analysed from the views of Career Development Program. Third, policy alternatives are developed for the improvement of job competency of public officials in Korea. To accomplish these objectives, this research adopts questionnaire survey method and Brain Storming approach in part. Seven policy alternatives are presented in this study. In order to successfully achieve the unification of South and North Korea, it is necessary to carry out a systematic study as to how the political and administrative systems of North Korea could be transformed into a democratic system. Such a transformation implies the fundamental reconstruction of a nation, accompanied by drastic changes in political and administrative systems. In the case of the former East Germany, the nations statehood itself changed as a result of German unification. Regardless of the method, if the product of Korean unification were a democratic, constitutional, free-market economy and a welfare society, North Korea would inevitably undergo a transformation of its state system. In particular, the transformation of human

피기백 인공수정체를 이용한 백내장 수술 후의 잔존 굴절 이상의 교정 1예

이종수,김영희,박정열,김성일,Jong Soo Lee,MD,PhD,Young Hee Kim,MD,Jung Yul Park,MD,Sung Il Kim,MD 대한안과학회 2014 대한안과학회지 Vol.55 No.12

Purpose: To report a case of corrected residual refractive error after cataract surgery with sulcoflex piggyback intraouclar lens (IOL). Case summary: A 77-year-old man was diagnosed as having hypermature cataract in the right eye and his corrected visual acuity in the same eye was hand-motion before surgery. Refractive error was +9.0 diopter (D) 6 months after conventional cataract surgery in the right eye. The authors performed additional cataract surgery using a piggyback method inserting a +10.0 D IOL in the ciliary sulcus. Four weeks after surgery, his refractive error was -1.25 D, visual acuity increased to 120/200 and 60/200 (with and without correction, respectively). No complication was observed during follow-up time and the patient was satisfied with his results. Conclusions: Correction of residual refractive error after cataract surgery with sulcoflex piggyback IOL is safe and effective. J Korean Ophthalmol Soc 2014;55(12):1890-1894