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이수영,곽재영,김성균,허성주 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.5
BACKGROUND: The purpose of this study is to examine physical characteristics of and initial biological properties to anodized titanium treated with poly(D,L-lactide-co-glycolide) (PLG) mixed with recombinant human bone morphogenic protein-2 (rhBMP-2). METHODS: Titanium specimens were prepared in groups of four as follows: group NC was anodized under 300 V as control; group PC was anodized then dropped and dried with solution 0.02 ml PLG; group D was anodized then dropped and dried with solution 0.02 ml PLG/rhBMP-2 (3.75 lg per disc); and group E was anodized then coated with 0.02 ml PLG/rhBMP-2 (3.75 lg per disc) by electrospray. Human osteoblastic-like sarcoma cells were cultured. Cell proliferation and alkaline phosphatase (ALP) activity test were carried out. Runx-2 gene was investigated by the reverse transcriptionpolymerase chain reaction. Immunofluorescence outcome of osteogenic proteins was observed. RESULTS: After 3 days, there were significantly higher proliferations compared rhBMP-2 loaded titanium discs with rhBMP-2 unloaded discs. The ALPase activity on rhBMP-2 loaded titanium discs was significantly higher than in rhBMP-2 unloaded discs. The expression level of Runx2 mRNA presented the highest on the PLG/rhBMP-2-coated surface. CONCLUSION: PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium.
Electro Diagnostic Nerve Feeling Assay in In-Vivo Muscles
이수영,백정기,강응준,이승준,이경 한국전시산업융합연구원 2018 한국과학예술융합학회 Vol.36 No.-
In the nerve networks, feeling/sensing controls are dependent on the neurotransmitters current. Signal detection for this current was searched using voltammetric circuits with three-electrode systems under cyclic voltammetry (CV) and chronoamperometry in the muscle and in-vivo brain. A working electrode was inserted into the rat brain’s 0.5-mm core under anesthesia, using a needle-type 0.3-mm-diameter macro. 10-mm-long inserted 5 mm deep and then cemented with a tooth binder and subsequently connected to the voltammetric workstation. Under the implanted conditions, various feeling senses for light, sound, and smell were examined. The results were applied to the detection of the spirit feeling current, five senses assay, and thinking neuro signals. The methods are found to be applicable in nerve detection, argument body control, and physiological behavior study.
이수영 제어로봇시스템학회 2013 제어로봇시스템학회지 Vol.19 No.4
전방향 영상 시스템은 한 장의 영상에 360도 모든 방향의 영상정보를 담을 수 있는 영상획득 시스템을 의미한다. 기하광학소자를 이용하여 사람의 눈이나 기존의 카메라로는 한 번에 볼 수 없는 매우 넓은 범위의 영상데이터를 효율적으로 얻을 수 있다. 또한 전방향 영상획득의 원리와 시점이 다른 두 영상, 즉 스테레오 영상획득의 원리를 적용한 전방향 스테레오 영상 시스템은 두 영상간의 정합을 통해 3차원 거리를 측정할 수 있다는 특징이 있다. 본 고에서는 전방향 스테레오 영상획득에 관해 기존에 제안된 연구결과들의 광학적 원리와 장단점을 조사, 비교하였다.
국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도
이수영,신주영,이영아,안창호,김예진,박필만,안혜련,이가연,정현환,Lee, Su Young,Shin, Ju Young,Lee, Young Ah,Ahn, Chang Ho,Kim, Yae Jin,Park, Pil Man,An, Hye Ryun,Lee, Ka Youn,Jung, Hyun Hwan 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.5
This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.
Acoustic character recognition via electrochemical in-vivo nerve feeling assay
이수영,이경 한국전시산업융합연구원 2019 한국과학예술융합학회 Vol.37 No.2
Aims ; In the future, brain computer interfacing technology expects to have a strong emergence of human. Methods ; Brain diagnosis technology depends on the measurement of neuronal currents, similar to the strength inside of a biological body strength of feeling sensation, and muscle activity. Results ; For that purpose, the present study investigated the electrochemical brain signals by using electrochemical three-electrode systems with cyclic voltammetry (CV) and chronoamperometry on the muscle surface. Conclusions ; Under these conditions, neuro application of character recognition was obtained, and developed results can be applied to acoustic intonation sensing assay.
땅콩 알레르기의 생쥐모델에서 Bifidobacterium (strain BGN4)의 항알레르기 효과
이수영,오세조,박석원,전계리,김지연,윤소윤,지근억 대한 소아알레르기 호흡기학회 2006 Allergy Asthma & Respiratory Disease Vol.16 No.2
Purpose : We underwent this study to evaluate the immunomodulating effects of intragastric administration of bifidobacterium(BGN4) using murine model of peanut allergy. Methods : C3H/HeJ mice were sensitized with 1 mg/dose of crude peanut(PN) extract with cholera toxin, intragastricly. Group I mice were pretreated with BGN4 for 14 days before PN snesitization, Group II were treated 14-days each before and during sensitization, Group III were sham treated PN sensitized mice, and Group IV were naive. PN-specific serum IgE levels and PN stimulated cytokine productions from splenocyte were measured in study groups. Results : PN-specific IgE levels were significantly lowered in Group II mice compare to Group I or Group III. PN-stimulated IL-4 productions were also remarkably depressed in Group II mice. The ratio of IFN-γ/IL-4 in Group II was the highest among experimental Groups, furthermore, PN-stimulated IL-12 production was only measured in Group II mice. The decreased levels of PN-specific IgE in Group II consist with decreased production of IL-4 and increased ratio of IFN-γ/IL-4 in this experiment. Conclusion : BGN4 treatment, especially pre-and-during PN sensitization, seemed to have anti-allergic effect by suppressing PN-specific IgE production. And lowered production of IL-4, increased production of IL-12, and the increased ratio of IFN-γ/IL-4 could be suggested as a part of immunomodulating mechanism of BGN4 treatment in this experiment. 목 적 : 땅콩알레르기의 생쥐모델을 이용하여 bifidobacreria(BGN4)의 위장관 투여에 의한 항원 특이적 IgE 항체 생산 조절과 비장세포의 항원 특이적 사이토카인 생성에 미치는 영향을 알아보기 위하여 본 연구를 시행하였다. 방 법 : 연구자들이 이미 확립한 IgE-매개성 땅콩 알레르기의 생쥐모델을 이용하였다. 이전의 연구 결과 가장 적절한 IgE, 생성 반응과 항원 특이적 사이토카인 생성이 유도된 땅콩 감작 농도인 1 mg/dose/mouse의 용량으로 실험 제 1, 2, 3, 7, 21일에 땅콩의 조항원을 위장관을 통하여 감작시켰다. 이 모델을 이용하여, BGN4가 제1형 알레르기 면역반응에 미치는 영향을 보기 위하여 BGN4의 경구 치료시기에 따라 실험군을 4군으로 나누어 진행하였다. 제1군은 땅콩 감작 전 2주간 BGN4를 투여한 군(pre/PN), 제2군은 땅콩 감작 전 2주 및 감작 시작 후 2주간의 총 4주간 BGN4를 투여한 군(Pre+Post/PN), 제3군은 땅콩 감작 전 2주간 PBS만 투여한 양성대조군(PBS/PN), 제4군은 땅콩 감작 및 BGN4 치료를 하지 않은 음성 대조군(naive)이었다. 연령 4주의 female C3H/HeJ strain의 생쥐를 각 군당 3-4마리씩 이용하여 동일한 실험을 2회씩 반복하였다. 각 군별로 4군간 실험 주별 혈청 땅콩 특이 IgE 농도을 비교하였고, 실험 4주에 얻은 비장세포의 72시간 배양을 통한 땅콩 특이적 IL-4, IL-10, IL-12, IFN-γ의 생성량을 비교 분석하였다.결 과 : 땅콩의 위장관 감작이 끝난 후 땅콩 특이 IgE의 농도는 제1군(pre/PN)과 제 3군(PBS/PN)에 비하여 제2군(pre+post/PN)에서 의미 있게 감소하였다. 그러나 땅콩 항원 자극에 따른 비장세포의 증식능은 감소하지 않았다. 한편 비장세포배양 부유액에서의 IL-4, INF-γ 및 IL-10은 제1군과 2군에서 모두 감소하였고, 특히 IL-4 의 생성 감소 및 IFN-γ/IL-4 ratio 증가는 혈청 땅콩 특이 IgE 생성의 감소와 비례하였다. IL-12는 감작 항원 자극에 의해서는 BGN4 감작 전 투여 및 감작기간 중 지속투여군인 제2군에서만 의미 있는 농도로 생성되었으며, Con A 자극에 의한 IL-12의 생성은 제2군 뿐 아니라 BGN4 전처치군인 제1군에서도 증가하였다.