Ganoderma lucidum IY 009조다당 분획들의 항암활성과 항보체활성간의 상호관계

이권행,이준우,한만덕,정훈,김영일,오두환,Lee, Kweon-Haeng,Lee, June-Woo,Han, Man-Deuk,Jeong, Hoon,Kim, Young-Il,Oh, Doo-Whan 한국미생물·생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.1

Antitumor polysaccharides were known to activate complement system and to increase specific serum proteins in mouse, and researcher reported that antitumor activity of polyasccharides might be correlated with their biological properties such as activation of complement system and increase of specific protein $L_{A}$, $L_{B}$ and $L_{C}$ within the mouse serum. In case of several Ganoderma lucidum, there was no correlation between their antitumor activities and their bioloical properties, but the antitumor activities against sarcoma 180 of the alkali extracted crude polysaccharide fractions of the Ganoderma lucidum IY 009. AS, T, AI and M were correlated with their bioloical properties such as anticomplementary activity and intensity of mouse serum protein $L_{A}$, $L_{B}$ and $L_{C}$.

CCl4 로 독성을 유발시킨 초대배양 간세포를 이용한 고등균류로부터 간세포 보호물질의 검색

이준우,한만덕,이권행 ( June Woo Lee,Man Deuk Han,Kweon Haeng Lee ) 한국균학회 1992 韓國菌學會誌 Vol.20 No.4

한국산 고등균류의 항보체활성 효과에 관한 연구

정훈,이준우,이권행 ( Hoon Jeong,June Woo Lee,Kweon Haeng Lee ) 한국균학회 1990 韓國菌學會誌 Vol.18 No.3

영지 균사체의 알칼리 추출물이 보체계와 망내계에 미치는 영향

이준우,정훈,정천희,이권행 ( June Woo Lee,Chun Hee Chung,Hoon Jeong,Kweon Haeng Lee ) 한국균학회 1990 韓國菌學會誌 Vol.18 No.3

Ganoderma lucidum IY 009로 부터 분리된 항암성 다당류의 약리 및 독성

이권행,이정옥,이준우,정훈,한만덕,정준호,오두환 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.2

G. lucidum IY 009의 균사체의 조다당 분획, AS는 sarcoma 180 cell에 직접적인 toxicity를 나타내지 않았으나, ICR mouse의 항체 생성과 marcrophage의 탐식능 및 분리된 macrophage로부터 superoxide ion 생성을 촉진시켰으며, AS분획의 항암활성은 macrophage 기능의 특이한 저해제인 trypan blue에 의해 저해 받았다. 또한 AS분획은 human serum중의 complement C3를 활성화시켰으며, AS분획의 항암활성은 보체계 활성화에 관여하는 2가 양이온의 chelator인 EDTA에 의해 저해 받았다. Mouse leukemia P388 및 L1210이 이식된 inbreed mouse, BDF1에서 Krestin은 항암활성을 전혀 나타내지 못하였으나, AS분획은 수명연장 및 고형암 증식억제 효과를 나타내었다. AS분획을 ICR mouse에 경구투여한 경우, 별 다른 독성을 보이지 않았으며, LD_50은 2,230 ㎎/㎏ 이상으로 나타났다. The highest antitumor activity was observed in water soluble AS fraction of the Canoderma lucidum IY 009. AS fraction did not show any cytotoxicity on sarcoma 180 cell but stimulated antibody production, opsonization of macrophage in ICR mouse and superoxide ion production from isolated macrophage. AS fraction activated complement C3 in human serum, and their antitumor activity was inhibited by EDTA, a chelator of cation related complementary activation. AS fraction exerted on prolong of life span and inhibition of tumor growth in the leukemia P388 or L1210 transplanted inbreed mouse, BDF1 but Krestin did not. AS fraction did not show any serious and lethal effects through oral administration on ICR mouse, and LD_50 of those was above 2,230 ㎎/㎏.

Ganoderma lucidum IY 009조다당 분획들의 항암활성과 항보체활성간의 상관관계

이권행,이준우,한만덕,정훈,김영일,오두환 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.1

항암활성 다당류들은 mouse의 보체계를 활성화시키고 serum내 특정 혈청 단백질을 증가시키는 것으로 알려져 왔다. 여러 연구자들의 의하면, 다당류의 항암활성은 보체계의 활성화와 mouse serum의 특정 단백질인 L_A, L_B 및 L_C등의 증가와 관련이 있다고 보고하였다. 6종의 Ganoderma lucidum의 균사체로부터 얻은 조다당의 항암활성과 항보체활성 사이에는 상관관계가 없었으나, G. lucidum IY 009의 균사체를 알칼리로 추출하여 얻은 조다당 분획- AS, T, AI 및 M 사이에서 sarcoma 180에 대한 항암활성이 높은 것이 항보체활성이 높게 나타났다. 한편 AS, AI 및 M을 투여한 mouse의 serum중의 L_A, L_B 및 L_C protein의 강도는 항암활성과 비례적으로 나타났으나, 열수추출 분획 H의 경우는 비례하지 않았다. Antitumor polysaccharides were known to activate complement system and to increase specific serum proteins in mouse, and some researcher reported that antitumor activity of polysaccharides might be correlated with their biological properties such as activation of complement system and increase of specific protein L_A, L_B and L_C within the mouse serum. In case of several Ganoderma lucidum, there was no correlation between their antitumor activities and their bioloical properties, but the antitumor activities against sarcoma 180 of the alkali extracted crude polysaccharide fractions of the Ganoderma lucidum IY 009. AS, T, AI and M were correlated with their bioloical properties such as anticomplementary activity and intensity of mouse serum protein L_A, L_B and L_C.

Ganoderma lucidum IY 009로 부터 분리된 항암성 다당류의 정제 및 구조분석

이권행,정훈,이준우,한만덕,최경숙,오두환 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.2

Ganoderma lucidum IY 009로부터 얻어진 조다당 분획, AS분획 0.2 g을 물에 녹여 DEAE cellulose(OH form)로 충진된 column에서 물, 0.1M NaHCO_3, 0.3M NaHCO_3, 0.5M NaHC0_3 및 0.5 N NaOH등으로 차례로 용출시켜 AS-1분획, AS-2분획, AS-3분획, AS-4분획 및 AS-5분획을 각각 얻었다. AS-1, AS-2, AS-3, AS-4 및 AS-5의 sarcoma 180에 대한 암저지활성은 각각 97.5%, 68.0%, 73.0%, 81.0% 및 66.0%로 물로 용출된 중성다당 분획인 AS-1가 가장 우수하였다. AS-1분획은 Sepharose CL-4B column에서 용출시켰을 때 단일 peak를 보였으며, 분자량은 약 580,000 dalton이었다. AS-1분획의 당 함량은 98.9%로 mannose 67.5%, xylose 22.5%, glucose 5.8%, galactose 1.8% 및 ribose 2.4%로 구성되어 있으며, I.R. spectrum 864.3㎝^-1에서 흡수파장이 나타나 α-xylomannan이라고 생각되었다. AS-1의 주요 methylated alditol acetate는 1,4-Di-0-acetyl-2,3,5-Tri-0-methyl-xylitol, 1,4,5-Tri-0-acetyl-2,3,6-Tri-0-methylmannitol 및 1,3,4,5-Tetra-0-acetyl-2,6-Di-0-methylmannitol로 확인되었고, molar ratio는 대략 1 : 2 : 1였으므로, AS-1의 core portion은 (1→4)linked mannopyranosyl unit로 매 3개의(1→14)linked mannopyranosyl residue마다 1개의 xylopyranosyl residue가 mannopyranosyl residue와 (1→3)결합을 하고 있는 structural unit를 가진 xylomannan으로 추정되었으며, structural unit의 중합도는 약 835였다. Alkali soluble(AS) fraction, revealed the highest antitumor activity of the alkali extracted fractions of G. lucidum IY 009, was loaded on DEAE cellulose(OH form) column. AS-1, AS-2, AS-3, AS-4 and AS-5 were obtained by stepwise elution with H_2O, 0.1 M NaHC0_3, 0.3 M NaHCO_3, 0.5 M NaHC0_3 and 0.5 N NaOH respectively, and their antitumor activities(I.R. %) against the sarcoma 180 were 97.5%, 68.O% 73.0%, 81.O% and 66.O% respectively. AS-1 observed highest antitumor activity was appeared as single peak on the Sepharose CL-4B column chromatography, and their molecular weight was about 580,000 dalton. The carbohydrate content of AS-1 was 98.9%, their monosaccharide consisted of 67.5% of mannose, 22.5% of xylose, 5.8% of glucose, 1.8% of galactose and 2.O% of ribose. AS-1 was assumed α-linkaged xylomannan having infrared absorption at 864.3 ㎝^-1. The main alditol acetates of AS-1 were identified as 1,5-Di-O-acetyl-2,3,4-Tri-0-methylxylitol, 1,4,5-Tri-0-acetyl-2,3,6-Tri-0-methylmannitol and 1,3,4,5-Tetra-0-acetyl-2,6-Di-0-methylmannitol by methylation analysis, and their molar ratio was 1 : 2 : 1. The core portion of AS-1 might be α-(1→4)mannopyranosyl unit branched with side chain, C1 of xylopyranosyl residue linked to C3 of every 3 mannopyranosyl units, and the degree of polymerization of structural unit in AS-1 was about 835.

Lentinus edodes IY105 알칼리 추출물의 보체계활성 및 항종양효과

이준우,정천희,정훈,이권행 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.6

경기도 광릉에서 채집한 자실체로부터 분리한 Lentinus edodes IY105균을 배양하여 얻은 균사체를 알칼리로 추출하여 얻은 물질이 면역계와 sarcoma 180이 이식된 mouse에 미치는 영향을 검토하였다. Lentinus edodes IY105 배양균사체의 알칼리 추출물은 항보체활성과 alternative complement hemolytic 활성을 가지고 있었고, carbon clearance 활성을 나타내는 것으로 보아 mouse의 망내계에 존재하는 macrophage를 활성화시켜 이물질에 대한 탐식기능을 증가시킴을 알 수 있었다. 또한, 이물질은 mouse의 용혈반 형성 세포수를 증가시키는 것으로 보아 항체생산 자극효과가 있는 것으로 추측되었으며, 종양세포인 sarcoma 180이 이식된 mouse의 암을 50% 정도 억제하는 효과를 나타내었다. 알칼리 추출물의 성분을 분석한 결과, 당 17%, 단백질 42%로 구성된 단백다당체가 주성분으로 단백다당체는 16종의 아미노산과 5종류의 당으로 구성되어 있었다. Alkali extract obtained from mycelia of Lentinus edodes IY105 was shown to have potent anticomplementary activity and alternative complementary activity in vitro. It was also shown to activate reticuloendothelial system of ICR mice in the carbon clearance. Amount of carbohydrates and protein of the extract were 17% and 42% respectively. It was found that carbohydrates were consisted of 5 monosaccharides and protein was consisted of 16 amino acids. Antitumor activity was observed in the alkali extract treated group of sarcoma 180 bearing ICR mice.

Isozyme patterns 차이에 의한 영지 , 치마버섯 및 동충하초의 계통분류

이준우,이권행 한국균학회 1991 韓國菌學會誌 Vol.19 No.2

To classify fungal species employed for pharmacological effects, mycelial proteins of six isolates of Ganoderma lucidum, five isolates of Schizophyllum commune and five isolates of Cordyceps spp. were separated on polyacrylamide gel to compare them by esterase, acid phosphatase, leucine aminopeptidase and peroxidase patterns. Similarity of isozyme patterns among the isolates of G. lucidum IY003, IY004, IY005 and IY008 was indicated over 70%, but that among the isolates of G. lucidum 1Y009, IY010 and others was indicated from 48% to 9%. Highest similarity of isozymes of S. commune was observed to be between 1Y803 and 1Y805, and similarity between these two isolates was 57%. Similarity among other isolates was shown to be from 40% to 56%. Isozyme patterns of Cordyceps spp. were comparatively different, even though they were originated from the same kind of insect as their isolate. Similarity between Cordyceps spp. IY901 and IY904, which was isolated from moths, was 67% and that of IY905 and IY909, which was originated from the larvae, was 42%. Similarity among other isolates was shown to be from 12% to 67%.