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Lysosome-related organelles extracts from egg-white for hypermelanosis reduction
윤지희,오민주,안지영,SEKHONSIMRANJEET SINGH,김양훈,민지호 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.2
Pigmentary disorders generally indicate that an increased amount of melanin, leading to darker color of skin, called hypermelanosis. Thus, several methods were studied for finding therapy of hypermelanosis until now in worldwide. In this study, the hypermelanosis reduction has been observed with daily lysosome-related organelles (LRO) extract from eggwhite. The scrub was used for enzyme extraction so as to maintain the stability of the extracted enzyme. The LRO extracts were divided and into two groups: free enzyme and fresh enzyme. Free enzyme was extracted on the first day from the LRO and fresh enzyme was extracted by scrub from LRO daily. The melanin dissolved phosphate buffer (pH 7.0) was treated by two groups of enzymes for a period of 30 days. The fresh enzymes were found to be more effective than the free enzymes. The activity of free enzymes decreases with time and as a result it could not degrade melanin after 36 days. The peroxidase activity was also measured and it matched with the results of previous studies. Therefore, the daily fresh enzymes displayed remarkable ability in hypermelanosis reduction than with free enzyme that lost its activity after some time. The present work can be potentially used for dermal disease in medical industry and also in cosmetic products.
서울 시내 PC방(Internet Cafe)의 세균분포와 항생제 내성 양상
윤지희,백한주,진한주,손예원,곽선영,양환진,홍은경,최성숙,하남주,Yun Ji-Hee,Back Han-Joo,Jin Han-Joo,Son Ye-Won,Kwak Sun-Young,Yang Hwan-Jin,Hong Eun-Kyung,Choi Sung-Suk,Ha Nam-Joo 한국미생물학회 2005 미생물학회지 Vol.41 No.3
본 연구는 PC방의 세균 오염 실태를 조사하기 위한 목적으로 2004년 7월${\~}$12월까지 서울시내에 있는 PC방(Internet Cafe)들 중에서 34곳을 표본으로 선정하여 연구를 수행하였다. 총 세균수를 측정한 결과, 키보드에서는 $9.0{\times}10^4$ CFU/ml, 마우스에서는 $2{\times}10$ CFU/ml, 그리고 화장실 손잡이에서는 $5{\times}10^3$ CFU/ml의 세균이 검출되었다. 이는 화장실 손잡이보다 키보드와 마우스에서 세균에 대한 오염정도가 더 심한 것을 의미한다. PC방에서 항생제에 대한 내성을 지닌 7개의 균주를 분리해 내었는데, 2개의 균주는 methicillin과 erythromycin에 대한 내성을 나타내었고, 5개의 균주는 gentamicin, ampicllin, cefotaxim, chloramphenicol에 대해서 내성을 나타내었다. 이들 균주들을 동정한 결과, 2 균주는 Staphylococcus aureus로 동정되었고, 4 균주는 Actinobacillus ureae로 동정되었으며. 나머지 1개의 균주는 Pasteurella multocida로 동정되었다. Pasteurella multocida와 Actinobacillus ureae는 잠재적인 병원균으로 이전에 Pasteurella ureae로 알려졌던 Actinobacillus ureae는 인간의 상부 호흡기에 드물게 공생하는 것으로 알려졌으며, Pasteurella multocida는 많은 가축의 비인두강 안에 존재하는 평범한 정상 균총을 구성하는 세균인 것으로 알려졌다. 항생제 내성에 대한 연구 결과 Staphylococcus aureus는 erythromycin과 methicillin의 혼합제에 대해서 $100\;{\mu}g/ml$ 이상의 높은 항생제 내성을 나타내었고, Pasteurella multocida와 Actinobacillus ureae는 gentamicin, ampicillin 에 대하여 $100\;{\mu}g/ml$ 이상의 높은 항생제 내성이 있음을 알 수 있었다. This study was performed to investigate the hygiene condition of PC room(internet cafe) in Seoul Korea. From July 2004 to December, 34 samples were collected, there's an average of $9.0{\times}10^4$ CFU/ml on keyboards, $2{\times}10$ CFU/ml on mouse and $5{\times}10^3$ CFU/ml on door konbs toilets, suggesting that keyboards and mouse are more contaminated than toilet door knobs. Seven antimicrobial resistant strains were isolated from PC Rooms. Two isolates were resistant to methicillin and erythromycin, while five isolates were resistant to gentamicin, ampicllin, cefotaxim, and chloramphenicol. By identification, these strains were identified as Staphylococcus aureus (2 strains). Actinobacillus ureae (4 strains) and Pasteurella multocida (1 strain), respectively. Pasteurella multocida and Actinobacillus ureae are potentially pathogenic bacteria. Actinobacillus ureae, formerly, known as Pasteurella ureae, is an uncommon of the upper respiratory tract in humans. Pasteurella multocida is a part of the normal flora in the nasopharynx of many domestic animals. We concluded that Staphylococcus aureus is highly resistant to erythromycin and methicillin over $100\;{\mu}g/ml$, while Pasteurella multocida and Actinobacillus ureae is highly resistant to gentamicin, ampicillinover over $100\;{\mu}g/ml$.
“새 정부 출범에 따른 초등교원정책의 쟁점과 과제”에 대한 토론문 2
윤지희 한국교원교육학회 2017 한국교원교육학회 학술대회자료집 Vol.2016 No.11
이번 학술대회의 “새 정부 출범에 따른 교원정책의 방향과 과제”라는 주제는 4 차 산업혁명 시대의 도래와 학령인구 감소, 새 정부 출범 등으로 교육현장의 교원 들도 앞으로의 국면이 어떻게 진행될지 큰 관심을 보이는 주제이기에 매우 시의적 절한 논의라고 생각합니다. 특히 박상완 교수님께서 발제해주신 “새 정부 출범에 따른 초등교원정책의 쟁점과 과제”는 변화하는 시대에 초등교사가 담당해야 할 역 할은 무엇이며, 전문성을 제고하기 위해 어떤 노력이 필요할지, 앞으로 초등교사에 게 필요한 정책은 무엇일지를 화두를 던지는 옥고라고 생각이 듭니다.
Engineering of non-mevalonate pathway for carotenoid production from C1 substrate
윤지희,김민선,오민규 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Formate is an environmental and sustainable C1 feedstock, which is soluble and could be synthesized from CO<sub>2</sub> or CO. In this study, we produced lycopene from formate using M. extorquens by engineering non-mevalonoate pathway, also called MEP pathway, for effective lycopene production. First, LMZ01 strain was constructed by deleting CrtCD, genes for consuming lycopene, and used as a parental strain, showing improved lycopene production. Then, dxs, a rate-limiting step of MEP pathway, was overexpressed under lac, tac, and mxaF promoter, respectively. Expression level was shown to be strong in the order of mxaF, tac, and lac promoter by expressing EGFP. Interestingly, while the mxaF promoter with strong expression level was ineffective, the tac promoter with moderate level showed 45% increase in lycopene production. This showed lycopene could be produced reasonably from formate and suitable expression level of MEP pathway is crucial.