태아 허파의 초기 발달 중 과립허파꽈리세포의 분화와 미세구조의 특징

유동화,김대중,김성수,김경용,이원복,Yoo, Dong-Hwa,Kim, Dae-Joong,Kim, Sung-Su,Kim, Kyung-Yong,Lee, Won-Bok 한국현미경학회 1999 Applied microscopy Vol.29 No.3

초기발생중인 태령 9주부터 20주의 사람 태아의 허파 7예에서 과립허파꽈리세포의 분화과정과 미세구조의 특징을 밝히고자 투과전자현미경으로 관찰한 결과는 다음과 같다. 1. 발생 9주와 12주에서 기관지 나무의 상피의 형태로는 과립허파꽈리세포의 분화를 확인할 수 없었지만 발생이 진행되면서 15주 후부터 허파꽈리를 형성할 부위의 상피는 점차 거짓중층상피에서 단순입방상피로 분화하였다. 2. 발생 9주에서 처음으로 뭇층판소체가 관찰되어 과립허파꽈리세포의 분화를 확인할 수 있었다. 이외에도 발생 $9\sim15$주 동안에 이 세포에 특징적인 세포질포함체, 과립상포함체, 뭇소포포함체 그리고 진한포함체 등의 세포내 포함물을 관찰할 수 있었다. 3. 발생 19, 20주에서 뭇층판소체는 수와 크기가 급격하게 증가하였지만 다른 세포내 포함물은 감소되어 있었다. 요약하면 사람 태아의 허파에서 발생 초기단계에 뭇층판소체를 가진 허파꽈리세포(또는 전구세포)가 관찰되었으며, 이외에도 특징적인 다양한 세포내 포함물을 가진 세포들이 관찰되었다. 그러므로 과립허파꽈리세포는 발생 9주 또는 그 이전부터 분화한다고 할 수 있다. The differentiation and ultrastructural characteristics of type II pneumocyte was investigated using 7 cases of human fetal lungs from 9 to 20 weeks of gestation by transmission electron micropscopy. The result obtained were as follows. 1. From the pattern of epithelium, type II pneumocyte was not discernable, but with the gradual development of gestation, the epithelium of the future pulmonary alveoli was transformed from pseudostratified columnar into simple cuboidal epithelium after 15 weeks of geatation. 2. The multilamellar body very specific to type II pneumocyte was observed at first at 9 weeks of gestation. Besides, another characteristics of the cell were also observed such as cytoplasmic inclusion body, granular inclusionbody,multivesicularinclusionbodyanddensebody. 3. The number and size of multilamellar body increased, but those of other inclision bodies decreased at 19 and 20 weeks of gestation. In summary, type II pneumocytes (or precursor cells) with multilamellar body and another characteristic inclusion bodies were observed in the human fetal lungs from 9 weeks of gestation. And so, it is suggested that the differentiation of type II pneumocyte starts at or before 9 weeks of gestation.

피부질환별 직접면역형광검사의 결과에 대한 분석: 11년간의 단일 기관 후향적 연구

유동화 ( Dong-wha Yoo ),이장훈 ( Jang-hoon Yi ),박경덕 ( Kyung-deok Park ),권혁진 ( Hyeok-jin Kwon ),김기호 ( Ki-ho Kim ),윤정호 ( Jung-ho Yoon ) 대한피부과학회 2024 대한피부과학회지 Vol.62 No.1

Background: Direct immunofluorescence (DIF) is a histochemical technique used to detect tissue-bound autoantibodies and diagnose various immune-mediated skin diseases. Objective: This study aimed to evaluate the sensitivity of DIF for each disorder, and the consistency between clinical, histopathological, and DIF results. Methods: A retrospective study was conducted in 194 patients who underwent skin biopsy and DIF testing at our hospital between January 2011 and December 2021. An antibody panel against immunoglobulin G (IgG), IgA, IgM, C3, C1q, and fibrinogen was used. The concordance rate and κ-coefficient between the clinical, histopathological, and DIF results were evaluated. Results: DIF was observed to be positive in 87 cases; 51 cases of immune-mediated bullous diseases, seven cases of connective tissue diseases (CTDs), 25 cases of vasculitis, and four cases of other diseases. The overall sensitivity of DIF for immune-mediated bullous diseases was 71.8%, which was higher than that of histopathology (64.8%). In CTDs and vasculitis, the overall sensitivities of DIF were 30.4% and 65.8%, respectively, which were lower than those of histopathology (73.9% and 84.2%, respectively). In addition, good concordance among the clinical, histological, and DIF results was observed. Conclusion: DIF is a useful diagnostic method, especially for immune-mediated bullous diseases, lupus erythematosus, and Henoch-Schonlein purpura. However, in other CTDs and vasculitis cases, the sensitivity of DIF is relatively low. Therefore, the diagnostic value of DIF along with clinical and histopathological findings will be maximized only when the DIF test is performed for appropriate diseases. (Korean J Dermatol 2024;62(1):18∼28)

지적장애인을 위한 재활승마 프로그램 개발

유동화 ( Yoo Dong-hwa ),황선환 ( Hwang Sun-hwan ) 한국융합과학회 2018 한국융합과학회지 Vol.7 No.1

연구목적: 많은 승마 클럽에서 운영되고 있는 재활승마 프로그램에는 여러 가지 문제가 제기되고 있다. 몇몇 클럽에서는 재활을 위한 전문교육을 수료하지 않았거나 지도자 자격증이 없는 코치들이 여전히 장애인들에게 승마를 교육하고 있다. 따라서 본 연구의 목적은 지적장애인들에게 적용이 가능한 유용한 재활승마 프로그램을 개발하고, 장애인들이 안전하게 승마를 즐길 수 있도록 하는데 있다. 연구방법: 이전에 개발된 세 가지의 대표적인 재활승마 프로그램과 교본 및 연구물을 심층적으로 분석하여 장애인에게 반드시 필요한 프로그램 요소를 발굴하고, 전문가들에게 검증을 받았다. 개발된 재활승마 프로그램은 다섯 단계로 구성되어 있으며, 각 단계의 프로그램은 상위 단계로 가기 위한 적절한 검증 과정이 포함된다. 첫 번째 단계는 기승자가 말을 무서워하지 않도록 하는 단계이다. 두번째 단계는 말과 소통하고 사랑하는 방법을 이해하며, 균형을 유지하는 법을 배운다. 세 번째 단계에서는 기승자가 말을 리드하면서 말과의 관계에서 상호 감정을 이해한다. 네 번째 단계는 어떠한 상황에서도 안정성을 유지할 수 있도록 기승자의 근력을 강화하는 단계이다. 마지막 단계에서는 말과의 소통뿐만 아니라 말에 대한 애정, 자신감, 균형과 리듬 및 근력을 최적화 한다. 매 단계는 두 가지 주요 목표가 있다. 하나는 말과의 커뮤니케이션 능력을 함양하는 것이고 또 다른 하나는 라이딩 기술을 향상시키는 것이다. 결론: 승마 프로그램은 재활이 필요한 사람들에게 가장 적합한 운동 중의 하나이다. 잘 개발된 재활승마 프로그램은 지적장애인에게 매우 유용하게 활용될 것이다. 본 재활승마 프로그램이 많은 승마클럽에서 활용되고 지적장애인의 재활뿐만 아니라 신체적, 정신적 건강에도 도움이 될 것이다. Purpose: The Rehabilitation Horse-Riding programs, which are run by many horse-riding clubs in Korea have various problems. In some clubs, general horse-riding coaches have never received any specialized education course for rehabilitation, or have no teaching license, but they still give training to disabled persons. In many cases, they sometimes use any horses that are not suitable for rehabilitation training. These kind of circumstances are very risky and can create serious accidents at any time. This article is to provide a useful Rehabilitation Horse-Riding problem, applicable to those mentally disabled persons, who may not be so good as able-bodied people, but have little problem in general physical activities, so that they can enjoy horse riding in a very safe way. Methods: This program is composed of 5 levels in total and each level has appropriate test process to move up to a higher level. And every level has main subject in two parts, one is set to communicate with horse for better horsemanship, and the other is set to enhance the riding skill. Results: In this Rehabilitation Horse-Riding program for mentally-disabled persons, we will have five(5) levels as follows; Level 1 is to help the rider, first of all, not to be afraid of horse that have very big build. Level 2 is to make the rider understand how to communicate with the horse and how to love the horse, and learn the sense of stability how to keep balance sitting on the wobbling horseback. Level 3 is to give the impression on the rider and horse, that the rider is always the leader in the relationship between them, and help the rider to learn the rhythmic sense, which comes next to balancing sense. Level 4 is to strengthen the muscle so that the rider can always balance in any unexpected situation such as irregular movement, or startle response of the horse. Level 5 is to train the rider to be a smart horseman, by being accustomed to the communication with the horse and affection for the horse, self-confidence, sense of balance and rhythm, and appropriate muscle power as well. Conclusion: A horse-riding program is one of the best activities for the people who need rehabilitation. And we believe that well-made program will be especially good for mentally-disabled persons. We hope that this program will be practically used by many horse-riding clubs and help those mentally-disabled persons for their rehabilitation, physically and mentally as well.

말의 보행형태에 따른 승마의 운동강도

유동화 ( Dong Hwa Yoo ),오유성 ( Sung Won Youn ),윤성원 ( Mun Jin Lee ),이문진 ( Yoo Sung Oh ) 한국스포츠정책과학원(구 한국스포츠개발원) 2014 체육과학연구 Vol.25 No.4

이 연구의 목적은 말의 보행형태인 평보, 경속보와 좌속보, 구보에 따른 승마의 운동강도(심박수, 산소섭취량, 호흡교환률, 대사량, 에너지 소비량)를 규명하는데 있다. 남녀 승마동호인 15명을 대상으로 4단계(평보, 경속보, 좌속보, 구보)의 과정을 5분씩 기승해서 얻은 심박수 및 호흡가스분석 자료(산소섭취량, 호흡교환률, 대사량, 에너지소비량)를 토대로 각 보행형태에 따른 평균값을 비교하였고, 성별에 따른 승마의 운동강도의 차이를 비교를 통해 다음과 같은 결론을 얻었다. 말의 보행형태별 운동강도에는 차이가 나타났으며, 말의 보행속도가 빨라질수록 운동강도가 증가하는 경향을 보였다. 경속보, 좌속보, 구보의 운동강도는 통계적으로 차이가 없었으며, 이들의 운동강도가 평보에 비해 높은 것으로 나타났다. 승마의 보행형태별 운동강도는 평보가 중강도, 속보와 구보가 고강도로 분류되었다. 말의 보행형태별 남녀 간의 운동강도는 유의한 차이가 나타나지 않았다. 단, 분당에너지소비량에는 남녀 간의 차이가 나타났는데 이러한 결과는 분당 에너지소비량이 남녀 간 체중의 차이에 의해 영향을 받기 때문인 것으로 판단된다. The purpose of this study was to examine the intensity of horseback riding as exercise (heart rate, oxygen intake, respiration exchange ratio, metabolism, energy expenditure) according to the tread of a horse such as walking, rising trotting, sitting trotting, and cantering. In this study, the subjects were 15 amateur horse riders. They participated in a 4-stage process(riding while the horse was walking, rising trotting, sitting trotting, and cantering) while wearing gas masks and heart rate belts for 5 minutes during each stage. Through this study, the average of heart rate, oxygen intake, respiration exchange ratio, metabolism, and energy expenditure in each stage of riding was attained and compared. The result of this study can be summarized as follows. First, the intensity of exercise by horse riding is significantly different depending on the tread of a horse. The intensity of exercise is increased as the speed of the horse’s movements increased. Trotting and cantering show higher intensity than walking. Among the different treads of a horse, walking can be categorized as having an intermediate intensity of exercise, whereas trotting and cantering are categorized into a higher intensity. However, there are no significant differences in intensity between trotting and cantering. Second, there are no significant differences between male female participants in terms of intensity of exercise, but there are differences in the energy consumption per minute. The weight differences between male and female participants may be what cause this difference.

승마 운동 시 말의 품종과 걸음걸이에 따른 보행 속도 연구

유동화(Yoo, Dong-Hwa),오유성(Oh, Yoo-Sung),천영진(Chun, Young-Jin) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.2

The purpose of this study was to calculate the speed according to each walking type using the thoroughbred and warmblood establish the standard speed. For horse riding, ten thoroughbreds and twelve warmbloods were used. The riders arrived at the equestrian court about an hour before the experiment day, and performed a preparatory exercise with a light bare hand gymnastic exercise. After about 20 minutes, they prepared the horses through a lightweight balance, a speedometer, The departure sensor and the arrival sensor were installed at a linear distance of 20 m in the section speed meter(MICRO GAIT WITTY). In order to apply the same criteria at the time of departure and arrival, the sensor was installed at the mouth height of the horse. The riders passed the 20 m distance five times according to the footwork method, and then calculated the mean and standard deviation. During the walk, if the horse deviated from the path or the footwork was changed, it was nullified and re-performed. The data obtained through the experiment were calculated by using the Excel program for 5 mean and standard deviation according to the 4 methods of walking. Based on the total 15 times of data for each breed, the speed difference. The independent t-test was conducted and the significance level was set to .05. The results showed that thoroughbred was slower than warmblood in all walking types and showed a statistically significant difference among all varieties except for canter(p<.05).

유전자 돌연변이가 확인된 모구 형질세포양 수지상세포 종양 1예

유동화 ( Dong-wha Yoo ),박경덕 ( Kyung-deok Park ),권혁진 ( Hyeok-jin Kwon ),김기호 ( Ki-ho Kim ),윤정호 ( Jung-ho Yoon ) 대한피부과학회 2023 대한피부과학회지 Vol.61 No.1

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and aggressive lymphoma with an overall incidence of 0.04 cases per 100,000 people. BPDCN is a hematopoietic clonal neoplasm that originates from plasmacytoid dendritic cell precursors. A 63-year-old man presented with multiple erythematous nodules over his whole body, including his face, trunk, and both upper and lower extremities that appeared 1 month ago. Skin biopsy showed diffuse dermal infiltration by monomorphic atypical lymphocytes with large, irregular nuclei and scant cytoplasms. Immunohistochemical staining was positive for CD4, CD56, and CD123. The karyotype test showed abnormalities in male chromosomes 47, XY, +8 [2]/46, and XY [25], and mutations in DNMT3A, TET2, SRSF2, and ATRX genes were identified in a next-generation sequencing (NGS)-based acute myeloid leukemia gene panel test. The patient was diagnosed with BPDCN and treated with a KALLA 1406 regimen; however, he died on the 17th day of treatment. (Korean J Dermatol 2023;61(1):57∼61)

CDMA 이동통신 시스템의 기지국 시스템 로딩 구조 설계

유동화(Yoo Dong Hwa),조무호(Cho Moo Ho),조철회(Cho Cheol Hye) 한국통신학회 1994 한국통신학회 학술대회논문집 Vol.1994 No.11

직접회로상의 선간 정전용량 계산을 위한 시뮬레이션에 관한 연구

유동화(Hwa Dong Yoo),박화식(Hwa Sik Park),황호정(Ho Jung Hwang) 대한전자공학회 1990 대한전자공학회 학술대회 Vol.1990 No.11

체외수정과 배이식에 의한 임신성공예에 관한 연구

구병삼,유동화,이규완,나중열,홍성봉,배인하,Ku, Pyong-Sahm,Yoo, Dong-Wha,Lee, Kyu-Won,Rha, Joong-Yul,Hong, Sung-Bong,Bae, In-Ha 대한생식의학회 1986 Clinical and Experimental Reproductive Medicine Vol.13 No.2

We have reviewed 59 cases of patients amoung 65 cases who underwent IVF and ET with reasonable indications irom 1984 and the results as follows. 1. Major indications for IVF and ET were tubal factor (40.7%), unexplained infertility (25.4%), endometriosis (15.3%), failed AID and AIH (10.1 %), and sperm abnormality (8.5%). 2. For superovulation of human oocytes, l00mg of clomiphene citrate and 75 IU of HMG used. The monitoring of oocyte maturation was bone by ultrasound examination and serum 17-${\beta}$ estradiol, LH values. The peak $E_2$ value was 956.36${\pm}$702.13 pg/ml. 3. The oocytes were obtained by laparoscopy 24-36 hours after the injection of HCG. 4. The mean numbers of follicles at laparoscopy was 3.06 and the successful rate of laparoscopy was 79.7%. 5. And 165 follicles were aspirated from which 98 oocytes were recovered, 59.4% of all follicles had at least one oocyte aspirated. 21.4% of the eggs were mature, 52.0% were moderate, 26.5%. were immature. 6. 67.3% of oocytes were cleaved and were transferred at 4-6 cell stages. 7. Four pregnancies including one chemical pregnancy and one spontaneous abortion were established by ${\beta}$-subunit, u-hCG and ultrasound examinations.

Epidemilogy of Respiratory Distress Syndrome

홍성봉(SB Hong),박용균(YK Park),이규완(KW Lee),유동화(DH Yoo) 대한산부인과학회 1986 Obstetrics & Gynecology Science Vol.29 No.7

1980년 1월 1일부터 1984년 12월 31일까지 고려대학교 의과대학 혜화병원 산부인과에서 최근에 분만한 총 4,879예중 호흡곤란증후군이 발생한 46예를 대상으로하여 아래와 같은 결론을 얻었다 1 발생빈도는 0 94%이었다 2 성별로는 남아가 80 4%로 많았다 3 체중별로 보면 2500gm미만에서 67 5%가 발생하였다 4 제태기간에 따른 빈도롤 보면, 37주이하에서 69 5%의 높은 빈도를 보였고, 동기간의 전체 분만에서 보면, 정상 제태기간에서는 0 33%만이 발생한 반면 조산아의 경우 337예중 30예인 8 90%에서 호흡곤란증후군이 발생하였다 5 산모의 연령별로 보면, 동기간의 전체분만중 30∼34세군이 2 17%, 35∼39세군이 2 08%의 높은 빈도를 보였고, 25∼29세군은 0 93%의 빈도를 보였다 6 3회 경산에서 2 35%, 1회 경산에서 1 05%로 초산의 0 90%보다 높았다 7 제왕절개술에서 1 24%로 질식분만의 0 83%보다 높은 빈도를 보였다 제왕절개술의 경우 그 적응증은 uterine hypoperfusion이 52 9%로 반수이상을 차지했다 8 쌍태아의 경우는 17 4%이고, 제2아에서 50 0%로 높은 빈도를 보였으나 통계학적 의의는 없었다 9 계절별로 보면 가을, 봄이 34 8%, 30 4%로 여름(13 0%)보다 높은 빈도를 보였다 10 호흡곤란증후군에서는 89 1%로 신생아가 사망했고, 그 사망시기는 2일이내가 87 8%를 차지했다 This is a review of 46 cases of Respiratory Distress Syndrome in 4879 deliveries at Hae Wha General Hospital, Korea University from January 1, 1980 to December 31, 1984 The results were summerized as follows: 1 Overall incidence of RDS was 0 94% 2 The incidence of RDS in male baby was remarkably higher than that of female baby (80 4% in male baby & 19 6% in female baby ) 3 The rate of RDS in low birth weight (under 2500Gm)was 67 5% 4 The rate of RDS in preterm (before 37wks) was 69 5% The overall incidence of RDS in preterm(8 90%)was higher than that of term bady (0 33%) 5 The overall incidence of RDS by maternal age was 2 17% in the age group of 30-34 years old, 2 08% in the age group 35-39 years old, and 0 93% in the age group of 25-29 years old 6 The incidence of RDS in para 3 (2 23%) and para 1 (1 05%) women was higher than that of primiparous(0 90%) 7 The incidence of RDS was higher in C/S (1 24%) than vaginal delivery (0 83%), the most common indication for C/S was uterine hypoperfusion, i e placenta previa and abruptio placenta (41 1%, 5 9%) and fetal distress (5 9%) 8 The rate of twin delivery in RDS was 17 4% and second baby was prevalent (50 0%), but there was no statistical significance 9 The incidence was higher in autumn and spring (34 8%, 30 4%) than summer season (13 0%) 10 The mortality was 89 1% & among these 87 8% were died in first 2 days after birth