http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
갈근에서 분리한 화합물의 $\alpha$-glucosidase와 $\alpha$-amylase 활성 저해 효과
박지희,백목련,이병회,연규환,유시용,김영섭,박상언,홍경식,Park, Jee-Hee,Baek, Mok-Ryeon,Lee, Byung-Hoi,Yon, Gyu-Hwan,Ryu, Shi-Yong,Kim, Young-Sup,Park, Sang-Un,Hong, Kyung-Sik 한국약용작물학회 2009 한국약용작물학회지 Vol.17 No.5
Compounds of isolated from roots extract of Pueraria thunbergiana were tested their inhibitory effects on $\alpha$-glucosidase and $\alpha$-amylase. Inhibitory activity of methylene chloride (MC) fraction and ethyl acetate (EA) fraction against $\alpha$-glucosidase showed more than 60% at a concentration of $500{\mu}g/m{\ell}$. Among the nine compounds tested on $\alpha$-glucosidase, biochanin A, (-)-tuberosin and calycosin from MC fraction and daidzein from EA fraction were stronger inhibitors than acarbose ($IC_{50}=530{\mu}g/m{\ell}$), and their $IC_{50}$ were 9, 144, 328 and $20{\mu}g/m{\ell}$, respectively. Biochanin A and (-)-tuberosin also inhibited $\alpha$-amylase activity as like as acarbose $IC_{50}=20.5{\mu}g/m{\ell}$), and their $IC_{50}$ were 22 and $348{\mu}g/m{\ell}$, respectively. Although daidzein was already known $\alpha$-glucosidase inhibitory effects, it was newly evaluated that biochanin A and (-)-tuberosin inhibited $\alpha$-glucosidase as well as $\alpha$-amylase, and that calycosin did $\alpha$-glucosidase.
김현기,최재석,유대석,최연희,연규환,홍경식,이병회,김혜진,김은주,박병근,정영철,김영섭,유시용 한국생약학회 2007 생약학회지 Vol.38 No.2
A rapid and practical HPLC assay was developed for quantitative analysis of saponins in Platycodi Radix. Seven saponin components in Platycodi Radix, i.e., deapioplatycoside E, platycoside E, deapioplatycodin D3, platycodin D3, polygalacin D2, platycodin D2 and platycodin D were successfully resolved on C18 column and detected by ELSD. Standard curves were linear over the concentration range 1~2,000 μg/ml (r 2 >0.992). Intra- and inter-day coefficients for variation of seven saponin components were <10% and limit of quantification of them were around 0.7~1.5 μg/ml, respectively. Using this method, contents of seven saponins in various plant materials under different cultivating conditions were estimated.
최재석,유대석,최연희,연규환,홍경식,이병회,김혜진,김현기,김은주,노성환,정영철,김영섭,유시용,Choi, Jae-Seok,Yoo, Dae-Seok,Choi, Yeon-Hee,Yon, Gyu-Hwan,Hong, Kyung-Sik,Lee, Byung-Hoe,Kim, Hye-Jin,Kim, Hyun-Ki,Kim, Eun-Ju,Roh, Seong-Hwan,Jeong 한국생약학회 2007 생약학회지 Vol.38 No.2
We investigated the contents of crude saponin in the decoctions of Platycodi Radix prepared by the different extraction processes. Each lyophilized decoctions prepared by the different protocol were suspended in water and partitioned with ethylacetate (EtOAc) and n-butanol (BuOH), which gave the EtOAc fraction, BuOH fraction and the remaining water fraction, respectively. The content of crude saponin, which was estimated as the amount of BuOH fraction, and the HPLC profiles of the BuOH fraction were analyzed, and compared each others. The crude saponin content in the decoctions were increased proportionally to the increment of the extraction time, amount of water, extraction temperature and the number of repetition for extraction. Consequently, the optimized conditions were deduced to be suitable and recommendable for the preparation of Platycodi Radix ; the water amount needed for the extraction be 15-20 volumes to crude material, temperature for extraction be optimal at $85{\sim}100^{\circ}C$, extraction time be more than 5 hours and the repetition of extraction was 2 times.
최춘환,Joo-Won Yoon,연규환,김영섭,유시용,석순자,Sunny Kang,김영호 한국생약학회 2012 Natural Product Sciences Vol.18 No.4
Mushrooms have a long history of use in traditional medicine, and hundreds of novel constituents in mushrooms with miraculous biological properties have been identified recently. Although diverse effects for medicinal use of mushrooms such as anticancer activity are proven, their reversal activities of drug resistance in cancer cells was rarely reported so far. In the search for novel medicinal use of mushrooms, we tested the multidrug resistance (MDR) reversal activities of diverse mushrooms collected from Korea. Among, the mushroom extracts tested, Cantharellus cibarius (M02) and Russula emetica (M12) revealed MDR reversal activities of paclitaxel in the P-glycoprotein (Pgp)-positive HCT15 and MES-SA/dX5 cancer cells, but not in the Pgp-negative A549 and MES-SA cancer cells. In addition, these mushrooms also enhanced the cytotoxicity of doxorubicin, another well-kwown Pgp-associated anticancer drug against MES-SA/DX5 cells, but not against MES-SA cells. Meanwhile, the cytotoxicity of cisplatin, a well-known Pgp-non-associated anticancer drug, was not affected by the mushrooms all the cells tested. From these results, we suspected that some ingredients of M02 and M12 have Pgp-associated MDR reversal activities.
갈근에서 분리한 화합물의 α-glucosidase와 α-amylase 활성 저해 효과
박지희,백목련,이병회,연규환,유시용,김영섭,박상언,홍경식 한국약용작물학회 2009 한국약용작물학회지 Vol.17 No.5
Compounds of isolated from roots extract of Pueraria thunbergiana were tested their inhibitory effects on α-glucosidase and α-amylase. Inhibitory activity of methylene chloride (MC) fraction and ethyl acetate (EA) fraction against α-glucosidase showed more than 60% at a concentration of 500μg/ml. Among the nine compounds tested on α-glucosidase, biochanin A, (-)-tuberosin and calycosin from MC fraction and daidzein from EA fraction were stronger inhibitors than acarbose (IC50=530μg/ml), and their IC50 were 9, 144, 328 and 20μg/ml, respectively. Biochanin A and (-)-tuberosin also inhibited α-amylase activity as like as acarbose IC50=20.5μg/ml), and their IC50 were 22 and 348μg/ml, respectively. Although daidzein was already known α-glucosidase inhibitory effects, it was newly evaluated that biochanin A and (-)-tuberosin inhibited α-glucosidase as well as α-amylase, and that calycosin did α-glucosidase.