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엄유리,김나현,김종윤,심수진,전권석,김만조 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Wild-simulated ginseng (WSG, Panax ginseng C. A. Meyer) in Korea which depends on an artificial forest growth method. Methods and Results : WSG samples were collected from 8 different regions in Korea in October, 2017, and these were analyzed by ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF MS) using optimized analytical methods to compare the metabolite patterns to cultivated regions. Furthermore, their metabolite compositions differed according to individual plant samples. The metabolite profiling data were processed by multi-variate statistical analyses such as PCA and PLS-DA to determine the differences among geographical origins. To identify significant metabolites according to regional variances of WSG, more sophisticated multi-variate statistical analyses such as metabolite selection should be performed. Conclusion : This metabolomics approach can also be applied to evaluate the overall quality of WSG, as well as to discriminate the cultivars for the medicinal plant industry.
엄유리,진미란,이대영,김창국,홍창표,이이,김옥태 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.6
Platycodon grandiflorum A. DC. contains a variety of triterpene saponins induced from the base structureof oleanane-type in roots. The β-amyrin synthase (bAS) plays an important role in controlling the biosynthesis oftriterpene saponins and we obtained the full-length cDNA of the gene, which is named PlgOSC1 in P. grandiflorum. To identify the PlgOSC1 products, we expressed it in heterologous yeast cells. Results of GC-MS analysis showed aunique peak that was consistent with that of the authentic β-amyrin standard with exactly the same retention time. Furthermore, the pattern of MS fragments of the peak was also exactly the same as that of the β-amyrin. In qRT-PCRanalysis of four organs of P. grandiflorum, no difference in PlgOSC1 expression level was observed; therefore, platycosidesderived from β-amyrin might be present in all P. grandiflorum tissues. In conclusion, PlgOSC1 encodes a bAS enzymethat catalyzes the first committed step of platycosides biosynthesis in P. grandiflorum.
Genetic diversity analysis of Glycyrrhiza uralensis using 8 novel polymorphic microsatellite markers
엄유리,Mei Lan Jin,이이,허목,차선우,정찬식,김성민,이종훈 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.2
Licorice plant (Glycyrrhiza spp.) is an important herb, but the major portion of the national demand is imported to Korea because the domestic production base is vulnerable. We performed basic molecular breeding research for domestic cultivation and production. All publicly available G. uralensis EST sequences, which totaled 56,089, were assembled into 4,821 unigenes and examined for microsatellites. Eight polymorphic microsatellite loci were identified and 16 G. uralensis and 6 G. glabra accessions, which were collected from different locations, were genotyped using the microsatellites. Genetic diversity within the accessions was estimated by construction of a dendrogram. The dendrogram was clustered into two groups. The results showed that there is a correlative genetic relationship between species. The microsatellite markers were found to be useful for diversity analysis as they are able to successfully distinguish the Glycyrrhiza accessions.
엄유리,이이,김성철,정연주,김금숙,최동욱,차선우,김옥태 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.4
Many ginsenoside biosynthesis-related genes have been identified in Panax ginseng. However, there is noreport of the analysis of changes in gene expression induced methyl jasmonic acid (MeJA) using DNA microarrayanalysis. To identify the genes related to ginsenoside biosynthesis, we harvested P. ginseng adventitious roots at 0,24, 72 and 120 h after MeJA treatment. At 120 h after MeJA elicitation, the contents of all ginsenosides increasedcompared to the control. We analyzed gene expression patterns in ginseng adventitious roots treated with MeJA usingDNA microarray analysis and selected candidate genes related to ginsenoside biosynthesis, including genes encodingsqualene synthase (SQS), squalene epoxidase (SE), dammarenediol-II synthase (DS), cytochrome P450 oxidase (CYP)and glycosyltransferase (GT). The expression patterns of these genes in MeJA-treated ginseng adventitious rootsobtained by quantitative RT-PCR were consistent with those obtained by microarray analysis. Therefore, DNA microarrayanalysis is an efficient tool for selecting candidate genes associated with secondary metabolite biosynthesis in plants.
엄유리,이문순,이이,석영선,Um, Yu-Rry,Lee, Moon-Soon,Lee, Yi,Seok, Yeong-Seon 한국연초학회 2011 한국연초학회지 Vol.33 No.1
Genus Nicotiana has 76 species including N. tabacum. These plants are used not only as a material for cigarette manufacturing but also as ornamental plant, medicinal plant, poisonous substance plant, and bug repellent plant. N. tabacum is used as a main material for cigarette manufacturing with N. rustica. N. sylvestris and N. alata is used as ornamental plants because of their beautiful flowers and N. rustica is used for bug repellent or pesticide because of its high concentration of nicotine. N. glauca, a tree tobacco, is used for bio-fuel production. N. tabacum is used as a popular model plant system for degeneration, regeneration, and transformation. N. benthamiana is also used as a model system for foreign gene expression by agroinfiltration. The transformation ability of tobacco plant is a good target for molecular farming. Hepatitis B virus envelop protein, E. coli heat-labile enterotoxin, diabetes autoantigen, and cholera toxin B subunit were produced using tobacco plants. Secondary metabolites of tobacco include nicotine, anabasine, nornicotine, anatabine, cembranoid, solanesol, linoleic acid, rutin, lignin and sistosterol, and they are used for various medicine productions which cannot be produced by organic synthesis for their complicated structures. In conclusion, we have to understand the applicability of tobacco plant in detail and study to enlarge the usage of the plants.
도라지 수집종의 형태적 특성과 SSR마커에 의한 유연관계 분석
엄유리,이이,Mei Lan Jin,이대영,이재원,김금숙,김창국,홍창표,김옥태 한국약용작물학회 2016 한국약용작물학회지 Vol.24 No.1
Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers. Methods and Results :We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups. Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.
인삼종자로부터 분리된 내생균의 동정과 식물생장 촉진 관련 활성의 평가
엄유리,김보라,정진주,정찬문,이이 韓國藥用作物學會 2014 한국약용작물학회지 Vol.22 No.4
Endophytes are microorganisms that live in the internal tissues of plants without harming the host plants. In this symbiotic relationship, the host plants provide nutrients and shelter to the endophytes, in turn, endophytes can promote the growth of host plants and act as a biological control agents against plant pathogens. Plant-microbe interactions like this are noted for natural methods for sustainable agriculture and environmental conservation. However, in spite of the infinite potential, there are only a few reports on the endophytes present in ginseng. In this study, we isolated and identified the endophytes from Panax ginseng seeds and evaluated the biological activities (IAA production ability, nitrogen fixation ability, phosphate solubilization capacity, siderophore production ability, and antifungal activities) of the endophyte isolates. Eight different endophytes were identified by 16S rRNA sequencing. Most of the endophytes have antibiotic and plant growth promoting (PGP) activities. Particularly, PgSEB5-37E have the highest antibiotic activity, both PgSEB5-37B and PgSEB5-37H have high PGP traits such as an abilities to produce IAA, solubilize phosphate and fix nitrogen. These results indicated that the endophytes from P. ginseng seeds may have applicable value to many industries. In order to use the isolated endophytes, quantitative analysis and field tests are needed to be performed.