겨울딸기(Rubus buergeri Miquel) 잎 추출물의 항산화 활성 및 항염증효과

김민선,양경월,김승영 한국생물공학회 2019 KSBB Journal Vol.34 No.4

Rubus buergeri Miquel contain various kinds of phytochemical substances and are known to have a wide range of physiological activities such as anti-inflammatory, antibacterial and anticancer effects. However, the underlying mechanisms of its inhibitory effects on inflammation in the RAW 264.7 macrophage cell have not yet been examined. In this study, we investigated the anti-oxidant activity and anti-inflammatory of Rubus buergeri Miquel leaf extract (RBM) on LPStreated RAW 264.7 cell. We found that pretreatment with RBM inhibited nitric Oxide (NO), prostaglandin E2 (PGE2) and proinflammatory cytokines. In addition, RBM significantly inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase- 2 (COX-2) on LPS-treated RAW 264.7 cell. Based on these results, we suggest that the RBM has the potential for use as an anti-oxidant and anti-inflammatory agents.

LPS로 자극된 RAW 264.7 세포에 대한 제주산 복분자 딸기 추출물의 항염증 효과

박태진,양경월,장성찬,김승영 한국생물공학회 2019 KSBB Journal Vol.34 No.3

Rubus coreabus Miquel are found in the southernpart of Korea and their fruits are known to have anticancer,antioxidant and anti-inflammatory properties. However, thesuppressive effect of Rubus coreanus Miquel stem and leafextract (RSL) on the expression of lipopolysaccharide (LPS) -induced inflammatory cytokines and mediators has not beenyet examined. So, we evaluated the anti-inflammatory effectof Rubus coreabus stem and leaf extract in LPS-stimulatedRAW264.7 cell. In this study, RSL inhibited the generation proinflammatorycytokines such as of nitric oxide (NO), prostaglandinE2 (PGE2), cyclooxygenase-2 (COX-2), inducibleNO synthase (iNOS) in a concentration dependent mannerwithout no observable toxicity in RAW 264.7 cells. Our resultsindicate that RSL could be a new source for the ingredients ofcosmetics and pharmaceuticals.

Gluconacetobacter sp. gel_SEA623-2를 이용한 Bacterial Cellulose 생산에 초산농도 및 유산균 혼합배양이 미치는 영향

김경민,김지현,양경월,Kim, Kyung min,Kim, Jihyeon,Yang, Kyong Wol 한국미생물학회 2014 미생물학회지 Vol.50 No.3

In this study, Gluconacetobacter sp. gel_SEA623-2 isolated from citrus that produces bacterial cellulose was used to examine the effect of initial concentration of acetic acid and mixed culture inoculated with Lactobacillus plantarum KCCM 80077 on productivity of bacterial cellulose. In mixed culture added with 0.5% acetic acid, the viable cell count increased from $2.4{\times}10^6CFU/ml$ to $1.1{\times}10^7CFU/ml$ after 14 days of culture, and total acidity was about 0.3% higher than single culture added with 0.5% acetic acid, which implies that additional lactic acid was produced by L. plantarum KCCM 80077. In single culture, although bacterial cellulose productivity was higher when the initial concentrations of acetic acid were 0.0% and 0.5%, than when it was 1.0%, there was no significant difference. However, in mixed culture, adding 0.5% acetic acid resulted in dry weight of $37.83{\pm}6.81g/L$ and thickness of $10.33{\pm}0.58mm$, showing a significant difference from that of single culture added with 1% acetic acid, $28.40{\pm}1.23g/L$ and $7.50{\pm}0.50mm$ (P<0.05). 본 연구에서는 bacterial cellulose를 생산하는 감귤에서 분리된 Gluconacetobacter sp. gel_SEA623-2를 이용하여 초기 초산농도 및 Lactobacillus plantarum KCCM 80077 유산균을 접종한 mixed culture가 bacterial cellulose 생산성에 미치는 영향을 알아보았다. 0.5%의 초산을 첨가한 mixed culture의 경우 초기 생균수가 $2.4{\times}10^6CFU/ml$에서 14일 동안 배양한 이후에 $1.1{\times}10^7CFU/ml$까지 증가하였고 total acidity가 0.5%의 초산을 첨가한 single culture와 비교하여 0.3% 가량 높게 측정되어 유산균에 의해 추가적으로 젖산이 생성된 것으로 판단된다. Single culture에서는 초기 초산농도가 0.0 및 0.5%일 때 1.0% 농도와 비교하여 상대적으로 높은 bacterial cellulose 생산성을 나타내기는 하였으나 유의적인 차이는 보이지 못하였다. 하지만 mixed culture에서는 0.5%의 초산을 첨가하였을 때 $37.83{\pm}6.81g/L$의 dry weight과 $10.33{\pm}0.58mm$의 thickness를 나타냄으로써 1%의 초산을 첨가한 single culture의 $28.40{\pm}1.23g/L$와 $7.50{\pm}0.50mm$의 생산량과 비교하여 유의적인 차이를 나타내었다(P<0.05).

백합뿌리 추출물의 멜라닌 생성 억제효과

윤훈석(Hoon Seok Yoon),양경월(Kyung-Wol Yang),김정은(Jung Eun Kim),김정미(Jeong Mi Kim),이남호(Nam Ho Lee),현창구(Chang-Gu Hyun) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.5

피부미백 소재를 개발하기 위해 멜라닌 함량, 세포내 tyrosinase 활성의 측정 및 Western blotting 실험이 수행되었다. 백합(Lilium Oriental Hybrid ‘Siberia’) 뿌리의 80% 에탄올 추출물로부터 얻은 에틸아세테이트 분획물(R-EA)은 α-melanocyte stimulating hormone(α-MSH)에 의해 멜라닌 생성이 유도된 B16/F10 흑색종 세포에서 농도 의존적으로 멜라닌 생성을 저해하였다. 정확하게 세포내 tyrosinase 활성과 멜라닌 함량은 에틸아세데이트 분획물 100μg/mL 처리 시 α-MSH 단독 처리군에 비해 각각 45%와 74%의 저해율을 보였다. α-MSH에 의해 멜라닌 생성이 유도된 B16/F10 흑색종 세포에서 단백질 발현양상을 살펴본 결과 TRP-1이 가장 많이 억제된 양상을 확인할 수 있었고 이 결과는 세포내 tyrosinase 활성저해보다 멜라닌 생성저해가 더 많이 일어난 것과 일맥상통하는 것이다. 이를 종합해 볼 때 p-coumaric acid와 resveratrol 함량이 백합뿌리의 에탄올 추출물에 비해 많이 함유된 에틸아세테이트 분획물은 멜라닌 생성 유도물질에 의해 촉진된 ERK의 활성화를 억제하는 피부미백 소재로서 그 가치가 입증된다고 사료된다. In order to develop a skin-whitening agent, melanin contents and intracellular tyrosinase activity were determined by western blotting. Ethyl acetate fractions of 80% ethanol extracts from lily (Lilium Oriental Hybrid ‘Siberia’) bulbs (R-EA) inhibited melanin synthesis in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)-treated B16/F10 murine melanoma cells. Intracellular tyrosinase activity and melanin contents were suppressed by 45% and 74%, respectively, in response to treatment with 100 μg/mL of R-EA. Examination of protein expression associated with α-MSH-induced melanogenesis showed that tyrosinase related protein (TRP)-1 was inhibited more strongly than tyrosinase, and these results were correlated with stronger inhibition of melanin synthesis than intracellular tyrosinase activity. Taken together, R-EA containing p-coumaric acid and resveratrol could be used as a hypopigmentation agent through suppression of sustained extracellular signal-regulated kinase (ERK) activation via melanogenic induction.

제주 당근 꽃의 항산화 및 항염증 활성

김수경(Su-Gyeong Kim),변후돈(Hoo-Dhon Byun),김상철(Sang Cheol Kim),양경월(Kyong-wol Yang),김정희(Jeong Hee Kim),한종헌(Jong-Heon Han) 한국생물공학회 2015 KSBB Journal Vol.30 No.2

The antioxidant and anti-inflammatory activities of extract and its fraction of Daucus carota var. sativa flower were studied in vitro. Extract and ethyl acetate fraction, butanol fraction of carrot flower showed radical scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH). We also investigated the effect of extract and ethyl acetate fraction, butanol fraction of carrot flower on NO production in a lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Extract and its fraction of carrot flower significantly inhibited NO production and this inhibitory action was not due to the cytotoxicity. This study suggests that extract and ethyl acetate fraction, butanol fraction of Daucus carota var. sativa flower could contribute to the chemoprevention and therapy of oxidative stress and inflammation.

Propionibacterium acnes에 대한 제주 자생식물 에탄올 추출물의 항균효과 검색

고미옥 ( Mi-ok Ko ),강희주 ( Hee-joo Kang ),황준호 ( Joon-ho Hwang ),양경월 ( Kyong-wol Yang ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.1

Propionibacterium acnes (P. acnes)는 여드름의 주요 병원성균으로 염증성 질환에 관련된 미생물 중에 하나이다. 본 연구에서는 여드름 주요 유발 균주인 P. acnes에 대하여 70% Ethanol로 추출한 제주 자생식물 추출물의 항균활성을 disc diffusion test 실험을 이용하여 측정하였다. 실험 결과 61개의 제주 자생 식물 추출물 중에서 45개 추출물의 항균 활성이 검출되었으며, 16개의 추출물은 항균 활성이 검출되지 않았다. 그중에서 여우 구슬(Phyllanthus urinaria L.) - 줄기·잎 추출물이 18.96 ± 0.69 mm로 가장 높은 항균 활성을 나타내었고, 참당귀(Angelica gigas Nakai) - 뿌리, 돌외(Gynostemma pentaphyllum (Thunb.) Makino), 참당귀(Angelica gigas Nakai) - 잎·줄기, 산박하(Isodon inflexus (Thunb.) Kudo), 차나무(Camellia sinensis L.) - 꽃, 그리고 로즈마리(Rosmarinus officinalis L.) - 줄기 추출물 순으로 높은 항균 활성을 나타내었다. 이러한 결과는 제주 자생 식물 추출물이 여드름 치료·예방의 항균제 및 기능성 화장품 천연 원료로써의 이용 가능성을 제안한다. Propionibacterium acnes, a major pathogens bacterium of acne, is one of the microflora associated with the inflammation. In this study, the antibacterial effects of 70% ethanol extracts from the 61 natural plants in Jeju against P. acnes were investigated using the disc diffusion method. The 45 natural plant extracts showed antibacterial effects and the 16 natural plants extracts didn’t show antibacterial effects. The Phyllanthus urinaria L. (stem and leaves) extract showed thr highest antimicrobial activity with 18.96 ± 0.69 mm clear zone of the growth of P. acnes, followed by Angelica gigas Nakai (roots), Gynostemma pentaphyllum (Thunb.) Makino, Angelica gigas Nakai (stem and leaves), Isodon inflexus (Thunb.) Kudo, camellia sinensis L. (flowers), Rosmarinus officinalis, etc. These results suggest that the natural plant in Jeju can be used as functional cosmetic biomaterials or antimicrobial agents against P. acnes.