제품디자인 프로세스에서 모델링 적정성에 관한 연구 - 디자인 개발 사례를 중심으로 -

신종헌,이길순 한국기초조형학회 2014 기초조형학연구 Vol.15 No.1

With the change of industrial society into the information-oriented society, many changes have occurred in terms of design process and design methodology. At the same time, with continuous changes in design paradigm, more abilities are now required from designers than any other times in the past. Processes are systemized and complemented by companies to react to markets because it is necessary to reduce the time to be taken for design development. Under this background, this study aims to focus on the 3D modelling stage, of the ordinary product design processes, that product shapes and structures are implemented, and discuss its appropriateness. 3D modelling on the computer has an advantage that it plays a role of bird's-eye view because it is three-dimensional, but it is two-dimensional anyway because it is a graphic on the monitor. This has also a disadvantage that there may occur an error between digital modelling and analogue mock-up, and finally loss occurs among designers and companies. This study aims to reduce the error that may occur during the product design 3D modelling, and based on its appropriateness, propose a modelling method that has a concept of preliminary filtering to guide product designers. Therefore, this study considers the concept and typical characteristics of modelling, current status of utilizing modelling at a working level, and its advantages and disadvantages, and based on these, proposes various methods depending on scale sketch, print check, and partial prototype. It seems that further researches need to be combined with the verification of the effect of identifying the appropriateness of 3D modelling. 산업 사회에서 정보화 사회로 변화하면서 디자인 프로세스와 디자인 방법론에도 많은 변화가 생겼다. 그리고 디자인의 패러다임도 지속적으로 변화하고 있어 현재는 과거 어느 때 보다 디자이너들에게 많은 능력을 요구하고 있다. 기업은 시장대응을 위해 디자인 개발 기간을 단축해야 하는 상황이 됨에 따라 프로세스를 체계화 하고 보완해 가고 있다. 이런 배경에서 본 연구는 일반적인 제품디자인 프로세스 중 제품의 형상과 구조가 구체화되는 3D모델링 단계에 집중하여 그 적정성에 대하여 논하고자 한다. 컴퓨터상의 3D모델링은 입체감을 통해 조감도 역할을 하는 장점은 있지만, 모니터 상의 그래픽이기 때문에 결국은 2차원에 해당한다. 이것은 디지털의 모델링과 아날로그의 목업(Mock-up) 사이에 오류가 발생할 수 있는 단점을 내포하고 있으며, 결국 디자이너와 기업에게 손실요소로 발생한다. 본 연구의 목적은 제품디자인 3D모델링을 할때 발생할 수 있는 오류를 최소화 하여 적정성을 바탕으로 한 사전 필터링 개념의 모델링 방법을 제안하여 제품 디자이너들에게 가이드 역할을 하는 것이다. 따라서, 본 연구에서는 모델링의 개념과 유형적 특징, 실무적 활용실태, 장단점을 고찰하고 그것을 바탕으로 스케일 스케치, 프린트 체크, 부분적 프로토타입 등 유형에 따라 다양한 방법을 제안한다. 향후 3D모델링 적정성의 효과에 대한 검증에 관한 연구가 병행되어야 할 것이다.

Genistein과 Daidzein이 대장암 HCT-116 세포의 성장에 미치는 영향

신종헌,강구성,김정옥,윤길숙,권태균,김정완,손윤경 대한병리학회 2006 Journal of Pathology and Translational Medicine Vol.40 No.1

Background : Genistein and daidzein are two major soybean isoflavones. They have received increasing attention because of their possible roles for cancer prevention. However, their mechanisms of action and molecular targets on the human colon cancer cells are not fully understood. Methods : Human colon cancer HCT-116 cells were treated with genistein and daidzein to investigate their effects on the cell growth and this was analyzed with MTT assay. TUNEL assay and Hoechst33342 stain were carried out to identify apotosis. Results : Daidzein was able to inhibit cell proliferation and induce apoptosis of the HCT-116 cells, but genistein didn't affect the cell growth. The ER antagonist ICI182780 didn’t attenuate the antiproliferative and proapoptotic effects of daidzein: this means the effect of daidzein on the HCT-116 cells may not be dependent on the ER pathway. The other soybean isoflavone, genistein, attenuated the effects of daidzein on the HCT-116 cells and its mechanism should be elucidated. Conclusions : These data suggest that daidzein may act as a preventive agent on human colon cancer, and its mechanism of action doesn’t involve the ER-dependent pathway.

Reaction of Thianthrene Cation Radical Perchlorate with Cumene Hydroperoxides

신종헌,김경태,Jongheon Shin,Kyongtae Kim Korean Chemical Society 1983 대한화학회지 Vol.27 No.2

아세토니트릴용액중의 티안트렌 양이온 라디칼 과염소산 염(1)은 Cumene hydroperoxide(4), p-chlorocumene hydroperoxide(2), p-nitrocumene hydroperoxide(3)와 상온에서 반응한다. 이 반응의 공통 생성물로서 티안트렌을 얻었으며 4로 부터 5-(4'-hydroxyphenyl) thianthrenium perchlorate (5), 2로부터 5-(5'-chloro-2'-hydroxyphenyl) thianthrenium perchlorate (7)와 5-acetonylthianthrenium perchlorate (6), 그리고 3으로부터 6만을 얻었다. 이 반응의 양관계는 1의 2몰이 티안트렌 1몰과 티안트렌이움 염(또는 염들) 1몰을 생성한다. 1에 대한 친핵성 활성도는 페놀 > > p-클로로페놀 ${\sim}$아세톤 > > p-니트로페놀 순서임이 밝혀졌다. Hydroperoxide의 산촉매 불균일 분해반응외에도 소량의 균일분해 반응생성물이 3과 4로부터 생성됨을 알았다. Reaction of thianthrene cation radical perchlorate (1) with cumene (4), p-chlorocumene (2), and p-nitrocumene (3) hydroperoxides in acetonitrile at room temperature afforded, inter alia, thianthrene as a common product and 5-(4'-hydroxyphenyl) thianthrenium perchlorate (5) for 4, 5-(5'-chloro-2'-hydroxyphenyl) thianthrenium perchlorate (7) and 5-acetonylthianthrenium perchlorate (6) for 2 and 6 for 3, respectively. Stoichiometry of these reactions showed that 2 moles of 1 gave rise to 1mole of thianthrene and 1 mole of thianthrenium salt (or salts). Nucleophilic reactivity to 1 was found to be in the order of phenol > > p-chlorophenol ${\sim}$ acetone > > p-nitrophenol. Apart from acid-catalyzed heterolytic decomposition of hydroperoxides, small amount of homolytic decomposition products were found from 3 and 4.

해양천연물의 구조결정 방법

신종헌 한국미생물 · 생명공학회 1999 생물산업 Vol.12 No.3

Lipopolysaccharide/Interferon-gamma에 의한C6 Glioma 세포주의 Nitric Oxide 생성 양상과Dexamethasone의 억제효과

신종헌,강구성,김지연,김선주,박지영,곽은경,손윤경 대한병리학회 2002 Journal of Pathology and Translational Medicine Vol.36 No.6

Background : Glial cell-derived nitric oxide (NO), and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to see the production of NO in lipopolysaccharide (LPS)/interferon-gamma (IFN-α)-treated C6 glioma cells and the effect of dexamethasone on NO production and apoptosis of LPS/IFN-α-treated C6 glioma cells. Methods : The apoptosis of LPS/IFN-α treated C6 glioma cell was examined with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method and the production of NO in culture medium was measured. The expression of iNOS mRNA was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The effect of the N-monomethyl L-arginine (NMMA) and dexamethasone on the apoptosis and NO production was also examined. Results : Inducible nitric oxide synthase (iNOS) mRNA and NO production were markedly increased in LPS/IFN-α- treated C6 glioma cells. The expression of iNOS mRNA arose at 3 hours, peaked at 12 hours, and declined 24 hours after LPS/IFN-α-treatment. Accumulation of NO derivatives in the culture media was increased at least upto 48 hours after LPS/IFN-α. The induction of iNOS expression and NO production in LPS/IFN-α-treated C6 cells was correlated with apoptotic cell death judged by TUNEL staining. After treatment of NMMA, one of the NOS inhibitors, NO production and apoptosis were markedly decreased. Dexamehasone treatment suppressed the NO production by concentration depenedent manner. Conclusions : From the above results it is concluded that the LPS/IFN-α induced apoptosis of C6 cells is mediated by iNOS-derived NO and NO production and apoptosis was suppressed by dexamethasone.

해양 미생물의 천연물에 대한 연구의 현황

신종헌 한국산업미생물학회 1994 생물산업 Vol.7 No.3

틱 장애 환자의 추적 조사

신종헌,정철호,김희철 대한소아ㆍ청소년정신의학회 1996 소아청소년정신의학 Vol.7 No.1

충남지역 자살자 특성에 따른 자살원인과 수단에 관한 비교연구

신종헌(Shin, Jeong-Heon),최은희(Choe, Eun-Hee) 한국지역사회복지학회 2009 한국지역사회복지학 Vol.0 No.31

Psammaplin A is a natural prodrug that inhibits class I histonedeacetylase

김동훈,신종헌,권호정 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.1

Histone deacetylase (HDAC) has been highlighted as one of key players in tumorigenesis and angio-genesis. Recently, several derivatives of psam-maplin (Psams) from a marine sponge have been known to inhibit the HDAC activity, but the molecular mechanism for the inhibition has not fully un-derstood. Here, we explored the mode of action of Psams for the inhibition of HDAC activity in the molecular and cellular level. Among the derivatives, psammaplin A (Psam A) showed the potent inhibitory activity in enzyme assay and anti-proliferation assay with IC50 value of 0.003 and 1 M, respectively. Psam A selectively induced hyper acetylation of histones in the cells, resulting in the upregulation of gelsolin, a well-known HDAC target gene, in a transcriptional level. In addition, reduced Psam A showed a stronger inhibitory activity than that of non-reduced one. Notably, glutathione-depleted cells were not sensi-tive to Psam A, implying that cellular reduction of the compound is responsible for the HDAC inhibition of Psam A after uptake into the cells. Together, these data demonstrate that Psam A could exhibit its activity under the reduced cobe a new natural prodrug targeting HDAC.

Binding Geometry of Free Base and Mn(III)meso-Tetrakis(N-methylpyridium-4-yl)porphyrin to Various Duplex and Triplex DNAs

성기웅,신종헌,김래영,장명덕,한성욱,이동진,조태섭 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.2

The binding mode of free base and Mn(III)meso-tetrakis(N-methylpyridium-4-yl)porphyrin (TMPyP and MnTMPyP) to various duplex and triplex synthetic polynucleotides was investigated by polarized light spectroscopy, specifically circular and linear dichroism (CD and LD) spectroscopy. Both TMPyP and MnTMPyP produced a similar positive CD spectrum in the Soret absorption region when bound to poly(dA)·poly(dT) duplex and poly(dA)·[poly(dT)]2 triplex. Considering that the third poly(dT) strand blocks the major groove, this result suggested that both porphyrins bound near the minor groove where the axial ligands of the central Mn(III) ion did not affect the binding mode. In contrast, TMPyP intercalated and MnTMPyP bound the exterior of poly(dG)·poly(dC) and poly(dI)·poly(dC) duplexes, as judged from the negative and positive CD signals, respectively. The CD signals for both intercalated TMPyP and externally bound MnTMPyP were greatly altered when bound to poly(dG)·poly(dC)·poly(dC)+ and poly(dI)·poly(dC)·poly(dC)+ triplexes, indicating that the binding modes of both porphyrins were affected by the presence of the third strand, poly(dC)+, which contrasted with the AT polynucleotides. This observation supported the conclusion that the external binding site of MnTMPyP at the GC and IC duplexes is the major groove. The reduced LD spectrum indicated that the angles of the two electric transition moments of both TMPyP and MnTMPyP in the Soret absorption region were 57° and 65° with respect to the local DNA helix axis when bound to the poly(dA)·[poly(dT)]2 triplex. On the other hand, a strong, wavelength-dependent reduced LD was observed in the Soret region for both porphyrins when associated with CGC triplex, and the excessive tilt degree prevented any reasonable calculation of the angles.