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신민기,전선민,구용의 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.2
Genetically modified (GM) rice varieties containingtraits such as tolerance to abiotic stress and resistanceagainst pests and diseases continue to be developed. However, contamination incidents from unauthorized GMrice varieties have been encountered. To date, no GM ricecrop has been authorized for consumption and/or commercializationin Korea. Therefore, to enhance safetymanagement of unauthorized genetically modified organisms(GMOs), accurate and reliable detection methods areneeded to identify GMOs in crops or products. In thisstudy, we developed rapid detection methods for GM riceevents (Bt63, KMD1, Kefeng6, Kefeng8, and LLRice62)using ultra-fast PCR system. Ultra-fast PCR is a state-ofthe-art technology and decreases PCR run-times dramatically. However, the ultra-fast PCR is not widely used inGMO analysis. Thus, we designed a detection method forfive events of GM rice and confirmed them by performingspecificity, sensitivity, and applicability assays. All resultsdemonstrate that the ultra-fast PCR system is a specific,sensitive, and reliable method to identify and monitor GMrice events. Additionally, it can be utilized as a rapid andsimple method for GMO analysis in crops or processedproducts. This study can be used as a reference for futureresearch on new analysis methods of unauthorized GMOs.
형상적응형 파지와 케이징 파지가 가능한 부족구동 기반 로봇 의수 메커니즘 개발
신민기,조장호,김기영,우현수 한국로봇학회 2022 로봇학회 논문지 Vol.17 No.4
This paper presents a simple and robust under-actuated robotic finger mechanism that enables self-adaptive grip, fingertip pinch, and caging grasp functions. In order to perform daily activities using hands, the fingers should be able to perform adaptive gripping and pinching motion, and the caging grasp function is required to realize natural gripping motions and improve grip reliability. However, general commercial prosthetic hands cannot implement all three functions because they use under-actuation mechanism and simple mechanical structure to achieve light-weight and high robustness characteristic. In this paper, new mechanism is proposed that maintains structural simplicity and implements all the three finger functions with simple one degree-of-freedom control through a combination of a four-bar linkage mechanism and a wire-driven mechanism. The basic structure and operating principle of the proposed finger mechanism were explained, and simulation and experiments using the prototype were conducted to verify the gripping performance of the proposed finger mechanism.
Detection method for genetically modified potato using an ultra-fast PCR system
신민기,전선민,구용의 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.9
Genetically modified (GM) potatoes having resistance to insects and viral diseases, low reducing sugar contents, and black spots for high quality continue to be developed. However, no GM potato has been approved as food or feed in the Republic of Korea as the country adheres to a zero-tolerance policy to unauthorized genetically modified organisms (GMOs). When the self-sufficiency rate is low, a detection method to assess GMOs in crops or other products is necessary. Therefore, a rapid method for two GM potato events (SPS-Y9 and EH92-527-1) using an ultra-fast PCR (UF-PCR) system has been developed, and its specificity, sensitivity, and applicability were demonstrated. UF-PCR can decrease the runtime of PCR by more than half of that needed in conventional methods. However, UF-PCR is not a common method for GMO analysis. This rapid detection method may be useful for GMO analyses in field conditions.
신민기,전선민,박종석 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.4
A calibration curve is required for reliable and accurate quantitative real-time PCR analysis of genetically modified (GM) organisms, necessitating the use of reference materials as calibrators. In this study, two types of DNA calibrators—plasmid DNA (pDNA) and genomic DNA (gDNA)—were used to quantify four GM soybean events (SYHT0H2, MON87751, DAS-44406-6, and DAS-81419-2). The PCR efficiency and linearity for the calibrators adhered to the CODEX guidelines. The conversion factor (Cf) was calculated as the ratio of copies of GM events to those of endogenous genes using the pDNA calibration curve. To assess the accuracy and repeatability of these assays, quantification at GM levels of 3% and 1% was performed. Based on our results, we believe that the pDNA calibrator assessed in this study can be used as a reference material for GMO quantitative analysis and can replace gDNA, especially considering the ease of management and advantages of mass production.