한우 보증씨수소 집단의 유전적 다양성 및 구조 변화 분석

신동현,김도현,오재돈 강원대학교 동물자원공동연구소 2018 동물자원연구 Vol.29 No.4

본 연구는 한우 보증씨수소 844두를 출생년도를 기준으로 8개 집단으로 분류하고, 각 개체들의 친자확인용 유전자 마커정보를 농협경제지주 한우개량사업소 홈페이지에서 제공 받아 유전적 다양성 및 구조 변화 분석에 활용하였다. 한우 보증씨수소 전체 집단의 대립유전자수(number of alleles)의 평균은 10.54개, 기대 및 관측 이형접합율(Hex, Hob)의 평균은 각각 0.764, 0.773, 다형성 정보량 지수(PIC)의 평균은 0.727 그리고 Fis의 평균은 –0.014로 확인되었다. 한우 보증씨수소 집단을 출생년도 별로 구분한 8개 집단의 유전적 다양성 및 구조 분석 결과, D집단(2005-2004년)의 기대이형접합율(0.777), 관측이형접합율(0.792) 그리고 다형성정보지수(0.740)가 가장 높은 것으로 확인되었다. C집단(2003-2004년)과 E집단(2007-2008년)에서는 기대이형접합율이 관측이형접합율 보다 큰 것으로 확인되었고, 나머지 그룹 모두에서는 관측이형접합율이 기대이형접합율 보다 큰 것으로 확인되었다. 대립유전자 출현빈도를 기반으로 유전적 조성과 구조를 추론하기 위해 STRUCTURE software를 이용하여 분석한 결과 세대가 지남에 따라 특정 유전적 성분의 변화 또는 비중의 증감을 확인 할 수 있었다. 이는 개량 목표를 설정하고 지속적으로 추진되고 있는 개량 사업이 한우 씨수소 집단의 유전적 구조 변화에 영향을 미치고 있음을 확인 할 수 있는 중요한 자료로, 한우 개량 사업의 효율적인 추진을 위해 유용하게 활용 될 것으로 사료된다. In this study, 844 Hanwoo proven bulls in South Korea (called KPN) were classified into 8 groups based on their birth year. Microsatellite (MS) marker information for paternity identification of each individual is provided at the homepage of the National Agricultural Cooperation Federation, Korea (NACF) and is mainly for the analysis of genetic diversity and structural changes. The polymorphism analysis of KPN whole groups revealed the average number of alleles in each marker (number of alleles), the expected heterozygosity (Hex), the observed heterozygosity (Hob), the polymorphism information content (PIC) and the Fis mean as 10.54, 0.764, 0.773, 0.727 and -0.014, respectively. For group D, with the birth year 2004-2005, the Hex and Hob were 0.777 and 0.792 respectively and the PIC was 0.740. The Hex of group C and D, with birth years 2003-2004 and 2007-2008, respectively, were greater than Hob. In all the other groups, Hob was greater than Hex. Genetic composition and structure were analyzed using STRUCTURE software. According to the analyzed results, the generation of Hanwoo groups showed changes in specific genetic components according to the flow. It was confirmed that the continuous improvement in the Hanwoo affects the genetic structure of the proven bulls group. The results of this study are expected to be used for enhancing the efficiency of Hanwoo improvement project.

전력소비 변동성에 대한 기온의 연속 · 누적 효과 분석

신동현 에너지경제연구원 2016 에너지경제연구원 수시연구보고서 Vol.- No.-

국내 지진기록의 통계적 분석에 기반한 스펙트럴 가속도 응답 예측기법

신동현,홍석재,김형준 한국지진공학회 2016 한국지진공학회논문집 Vol.20 No.1

This study suggests a prediction model of ground motion spectral shape considering characteristics of earthquake records in Korea. Based on the Graizer and Kalkan’s prediction procedure, a spectral shape model is defined as a continuous function of period in order to improve the complex problems of the conventional models. The approximate spectral shape function is then developed with parameters such as moment magnitude, fault distance, and average shear velocity of independent variables. This paper finally determines estimator coefficients of subfunctions which explain the corelation among the independent variables using the nonlinear optimization. As a result of generating the prediction model of ground motion spectral shape, the ground motion spectral shape well estimates the response spectrum of earthquake recordings in Korea.

In Silico Evaluation of Deleterious SNPs in Chicken TLR3 and TLR4 Genes

신동현,송기덕 한국가금학회 2018 韓國家禽學會誌 Vol.45 No.3

The innate immune recognition is based on the detection of microbial products. Toll-like receptors (TLRs) located on the cell surface and the endosome senses microbial components and nucleic acids, respectively. Chicken TLRs mediate immune responses by sensing ligands from pathogens, have been studied as immune adjuvants to increase the efficacy of vaccines. Single nucleotide polymorphisms (SNPs) of TLR3 and TLR4 genes in chicken were associated with resistance and susceptibility to viral infection. In this study, SNPs of chTLR3 and chTLR4 genes were retrieved from public database and annotated with chicken reference genome. Three-dimensional models of the chTLR3 and chTLR4 proteins were built using a Swiss modeler. We identified 35 and 13 nsSNPs in chTLR3 and chTLR4 genes respectively. Sorting Intolerant from Tolerant (SIFT) and Polymorphism Phenotyping v2 (Polyphen-2) analyses, suggested that, out of 35 and 13 nsSNPs, 4 and 2 SNPs were identified to be deleterious in chTLR3 and chTLR4 gene respectively. In chTLR3, 1 deleterious SNP was located in ectodomain and 3 were located in the Toll / IL-1 receptor (TIR) domain. Further structural model of chTLR3-TIR domain suggested that 1 deleterious SNP be present in the B-B loop region, which is important for TIR-TIR domain interactions in the downstream signaling. In chTLR4, the deleterious SNPs were located both in the ectodomain and TIR domain. SNPs predicted for chTLR3 and chTLR4 in this study, might be related to resistance or susceptible to viral infection in chickens. Results from this study will be useful to develop the effective measures in chicken against infectious diseases.

밀란병기 이내로 진단된 간세포암 환자의 사망 원인분석

신동현,이현우,곽금연,백용한,최문석,이준혁,고광철,백승운,강원석 대한간암학회 2016 대한간암학회지 Vol.16 No.2

Background/Aims: Hepatocellular carcinoma (HCC) is a unique condition where the cause of death might not only be due to progressive cancer, but also from liver failure. We evaluated specific causes of death for HCC patients who were initially diagnosed within the Milan criteria. Methods: A retrospective cohort of 147 patients with mortality who were initially diagnosed with HCC within the Milan criteria between January 2008 and December 2012 at a single institution was reviewed. Results: During follow-up, 104 patients (70.7%) experienced one or more cirrhotic complications, such as ascites, variceal bleeding, or hepatic encephalopathy. Near mortality, cancer progression (exceeding the Milan criteria) was recorded for 102 patients (69.3%), while cirrhosis progression (greater than two-point increase in Child-Pugh score) was noted in 110 (74.8%) patients. Alphafetoprotein, protein-induced by vitamin K antagonist-II levels and treatment modality were associated with cancer progression, while age and Child-Pugh class were associated with cirrhosis progression. There were 61 patients with in-hospital mortality; cancer progression plus liver failure was noted in 34 patients (55.7%), liver failure without cancer progression was seen in 20 patients (32.8%), and only four patients (6.6%) showed mortality from extrahepatic metastasis without liver failure. Conclusions: Among HCC patients who were diagnosed within the Milan criteria, most of them had cirrhosis progression near mortality, and significant proportion died without uncontrolled cancer growth, mainly due to liver failure. These findings show the importance of liver function that should be considered in managing HCC patients.

시공정밀도에 따른 비구조용 콘크리트벽돌 조적벽체의 프리즘 압축강도

신동현,김형준 대한건축학회 2020 大韓建築學會論文集 : 構造系 Vol.36 No.2

Prism compressive strength is the most influential parameter to evaluate the seismic performance of non-structural concrete brick masonrywalls, and is affected by the practice and workmanship of masonry workers. This study experimentally investigates the influence ofworkmanship on prism compressive strength throughout the compressive test with prism specimens constructed according to masonryworkmanship. To do this, the workmanship is categorized into good, fair, and poor conditions which are statistically evaluated with thicknessand indentation depth of bed-joints. Then, the effect of workmanship on the structural properties of masonry prisms is evaluated byinvestigating relations between properties such as their compressive strength, elastic modulus and numerical parameters such as thickness,filling of bed-joints. This study demonstrates that the indentation depth is more important parameter for structural properties of masonryprisms and masonry prisms with loss in bed-joint area less than of 7% can be in fair condition. 본 연구에서는 조적공의 시공정밀도가 프리즘 압축강도에 미치는 영향을 분석하기 위하여 시공정밀도에 따른 조적 프리즘 공시체를 제작하고 압축실험을 수행하였다. 이를 위하여, 시공정밀도는 양호, 보통, 및 불량으로 구분하고 줄눈의 두께와 만입정도에 대해 통계분석을 하였으며, 조적 프리즘의 물성치와 조적프리즘 시공정밀도의 관계를 분석하였다. 분석결과 조적프리즘의 줄눈 두께보다 만입 정도가 프리즘 압축강도에 민감하게 영향을 미쳤으며, 양호 정도의 시공정밀도를 확보하기 위해서는 7% 미만의 줄눈 채움 정도 손실이 요구되었다.

수중 음파통신 기술과 동향

신동현,박성준,김창화 대한전자공학회 2018 전자공학회지 Vol.45 No.5

바이오에탄올의 CO₂저감 효과에 대한 경제성 분석

신동현,Sin, Dong-Hyeon 대한석유협회 2005 석유와 에너지 Vol.2005 No.11

비선형 증분동적해석을 통한 철골 중간모멘트 골조의 붕괴성능 평가

신동현,김형준 한국구조물진단유지관리공학회 2014 한국구조물진단유지관리공학회 논문집 Vol.18 No.2

Steel intermediate moment frames (IMFs) have been generally used as seismic load resisting systems (SLRSs) of a building to provide resistances against strong ground shaking. However, most of low and mid-rise steel buildings in Korea were constructedduring pre-seismic code era or before the introduction of well-organized current seismic codes. It has been recognized that the seismic performance of these steel IMFs is still questionable. In order to respond to such a question, this study quantitatively investigates the seismic capacities of steel IMFs. Prototype models are built according to the number of stories, the levels of elasticseismic design base shear and the ductilities of structural components. Also, the other prototype models employing hysteretic energy dissipating devices (HEDDs) are considered. The collapse mechanism and the seismic performance of the prototype models are then described based on the results obtained from nonlinear-static and incremental-dynamic analyses. The seismic performanceof the prototype models is assessed from collapse margin ratio (CMR) and collapse probability. From the assessment, the prototype model representing new steel IMFs has enough seismic capacities while, the prototype models representing existing steel IMFs provide higher collapse probabilities. From the analytic results of the prototype models retrofitted with HEDDs, the HEDDs enhance the seismic performance and collapse capacity of the existing steel IMFs. This is due to the energy dissipating capacityof the HEDDs and the redistribution of plastic hinges. 철골 중간모멘트골조는 강한 지반운동에 대하여 적합한 저항능력을 확보하기 위한 지진력저항시스템으로서 일반적으로 사용되고 있다.하지만 국내의 대다수 중·저층 철골건축물은 내진설계가 도입되기 이전에 건설되었거나 현재의 내진설계기준의 요구조건을 준수하지 않은 것들로, 이러한 건물들이 가지는 내진성능에는 의문점이 존재한다. 이와 같은 문제점의 인식에 기반하여 본 연구에서는 국내 철골 중간모멘트골조의 내진성능에 대한 정량적 제시를 목표로 우선 층수 종류, 지진에 대한 보유내력, 부재 연성도, 제진장치의 유무를 변수로 하여표본 건물을 설계하였다. 표본 건물의 내진 성능과 붕괴 매커니즘은 비선형 정적해석과 증분동적해석으로부터 획득한 붕괴여유비와 붕괴확률을 이용하여 분석하였다. 해석결과를 통하여 현행 국내기준에 따라 내진설계된 신축건물은 설계지진에 대해 충분한 내진성능을 가졌으며, 이에 반해 구조부재의 연성저감이 발생하거나 낮은 설계 밑면전단력에 대한 저항력을 가진 기존건물의 경우에는 높은 붕괴확률을 가지며목표로 한 내진성능을 만족시키지 못하는 것으로 나타났다. 이와 같은 내진성능을 충족시키지 못하는 내진설계 도입 이전의 건물에 대해서에너지 소산장치를 통해 보강하게 되면 장치의 에너지 소산능력뿐만 아니라 소성힌지의 재분배를 통해 붕괴확률 및 내진성능이 신축건물수준으로 향상되었다.

해저 퇴적토 내 유기성 메탄 회수를 위한 가스하이드레이트 치환기법 연구

신동현,박대원 유기성자원학회 2018 유기물자원화 Vol.26 No.4

In this study, the effect of physico-chemical factors (e.g., pressure, electrolyte, and organic matter) in the gas hydrate deposit on CH4-CO2 replacement process was investigated experimentally. The higher initial pressure during gas injection led the higher reaction rate at the first time, but finally it did not. Electrolytes and organic matter have some effects on reforming process after dissociation of gas hydrate. It is expected that further research using real marine sediments with actual gas hydrate will enable the development of technologies applicable to the characteristics of domestic seabed geology. Ultimately, it is expected that it will be possible to recover and utilize methane as an organic resource through application of domestic gas hydrate deposit in the Ulleung Basin, East Sea. 본 연구에서는 압력, 전해질, 유기물 등 가스하이드레이트 부존 지역의 물리화학적 인자가 치환반응에 미치는 영향을 실험적으로 규명하였다. 가스 주입 시 초기 압력이 높을수록 초반 반응속도는 향상되었으나 시간이 지남에 따라 치환효율이 일정한 값으로 수렴하였다. 전해질과 유기물 등의 경우 가스하이드레이트 해리 후 재생성 과정에서 일부 영향을 미치는 것으로 밝혀졌다. 향후 실제 유기성 메탄 하이드레이트가 부존된 퇴적토를 활용한 추가 연구를 통해 국내 해저 지질특성에 적용 가능한 기술 개발이 가능할 것으로 기대된다. 궁극적으로 향후 동해 울릉 분지의 가스하이드레이트 매장 지역 현장 적용을 통해서 유기성 자원인 메탄을 회수하고 활용할 수 있을 것으로 예상된다.