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박현근,이정은,이령아,김광호 대한대장항문학회 2008 Annals of Coloproctolgy Vol.24 No.6
Purpose: DNA methylation is a major epigenetic mechanism for modification of genetic expression without a change in the DNA sequence. MBD2 (methyl-CpG-binding domain 2 protein) belongs to a family of enzymes concerning of DNA demethylation and suppresses the hypermethylation of the CpG island and DNA transcription. In this study, we investigated the change of MBD2 expression in the blood and tissue of colorectal cancer patients and compared the two expression levels. Methods: The 68 patients included in this study were patients with colorectal cancer who had undergone surgery at our hospital, and 50 other patients with no malignant disease were recruited from normal populations. Total RNA samples were isolated from whole blood samples and cancer tissues of specimens using a TRI REAGENT BD kit. MBD 2 expression was measured by real-time quantitative reverse transcription-polymerase chain reaction assays. Results: The mean age was older in the case group than in the control group. The mean expression level of MBD2 in blood was not different between the two groups. In the case group, the tissue MBD2 expression was lower than the blood MBD2 expression under all conditions, and that difference was statistically significant (P<0.01). The expression of MBD2 in cancer tissue showed a negative correlation with that in the blood of cancer patients, correlation coefficient of R=0.073, but that result was not statistically significant (P=0.611). Conclusions: The blood MBD2 expression was statistically the same in the cancer and the control groups. In the cancer group, blood MBD2 expression was significantly higher than tissue MBD2 expression. The reverse correlation between blood MBD2 expression and tissue MBD2 expression in cancer patients suggests that MBD2 may affect the mechanism of carcinogenesis. Purpose: DNA methylation is a major epigenetic mechanism for modification of genetic expression without a change in the DNA sequence. MBD2 (methyl-CpG-binding domain 2 protein) belongs to a family of enzymes concerning of DNA demethylation and suppresses the hypermethylation of the CpG island and DNA transcription. In this study, we investigated the change of MBD2 expression in the blood and tissue of colorectal cancer patients and compared the two expression levels. Methods: The 68 patients included in this study were patients with colorectal cancer who had undergone surgery at our hospital, and 50 other patients with no malignant disease were recruited from normal populations. Total RNA samples were isolated from whole blood samples and cancer tissues of specimens using a TRI REAGENT BD kit. MBD 2 expression was measured by real-time quantitative reverse transcription-polymerase chain reaction assays. Results: The mean age was older in the case group than in the control group. The mean expression level of MBD2 in blood was not different between the two groups. In the case group, the tissue MBD2 expression was lower than the blood MBD2 expression under all conditions, and that difference was statistically significant (P<0.01). The expression of MBD2 in cancer tissue showed a negative correlation with that in the blood of cancer patients, correlation coefficient of R=0.073, but that result was not statistically significant (P=0.611). Conclusions: The blood MBD2 expression was statistically the same in the cancer and the control groups. In the cancer group, blood MBD2 expression was significantly higher than tissue MBD2 expression. The reverse correlation between blood MBD2 expression and tissue MBD2 expression in cancer patients suggests that MBD2 may affect the mechanism of carcinogenesis.
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Escherichia coli K12의 막단백질 형성과 페니실린 민감성에 대한 산소의 능동적 역할
박현근,한홍의 한국미생물학회 1986 미생물학회지 Vol.24 No.3
Membrane proteins of facultatively anaerobic Escherichia coli K12 which was logarithmically grown in aerobiosis and anaerobiosis were compared on 5 to 10% liner gradient gel electrophoresis (Na Dod $SO_4 -PAGE$). Membrane proteins were formed as different patterns between aerobiosis and anaerobiosis. Among them, 91Kdal protein (pbp1a) was not synthesized in aerobiosis and 60Kdal protein (fts cluster), in anaerobiosis. Thereby cells cultured aerobically were differenciated as diversiform cell shape, comparing cells cultured anaerobically and the latter were resistant to penicillin G. Thus it is believed that in facultative anaerobes atmospheric oxygen regulated the synthesis of membrane proteins and even the expression of equivalent genes, and moreover alleviated the resistance to an antibiotic penicillin.
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박현근,양문,한홍의,Park, Hyeon-keun,Yang, Moon,Han, Hong-ui 한국미생물학회 1997 미생물학회지 Vol.33 No.4
아미노산이 김치유산균에 미치는 영향을 검토하였다. 발효온도 $15^{\circ}C$에서 형태가 다른 집락을 73개 분리하였다. 이 중에서 69.9%가 덱스트란 생성균주이었으며, Leuconostoc 속이 4.1%, Lactobacillus 속이 65.8%를 차지하였다. Tyrosine 500 ppm이 되도록 첨가한 배지에서 분리균주의 생장은 완전히 억제되었다. 그러나 실제로 이 아미노산을 첨가하여 만든 김치에서 분리된 총 집락수는 58개 이었고, 이 중에서 덱스트란 생성균주가 70.7%로 큰 변동이 없었으나 대신에 Leuconostoc 속이 41.4%로 증가하였고, Lactobacillus 속은 29.3%로 감소하였다. 각 속에서 우점종은 Leu. mesenteroides와 Lac. minor이었다. 따라서 실제 김치에서 tyrosine은 Lactobacillus 속의 생장을 억제하는 효과가 있었다. Effects of amino acids on the lactic acid bacteria in kimchi were studied. 73 different lactic acid bacteria have been isolated during the kimchi fermentation at $15^{\circ}C$. Among these bacteria, dextran formers were occupied by 69.9%, of which Leuconostoc and Lactobacillus were 4.1% and 65.8%, respectively. All isolates didn't grow in a medium added with 500 ppm of tyrosine, whereas such an inhibition was not exhibited in kimchi with the same concentration of tyrosine. In kimchi added with tyrosine the lactic acid bacteria were less diverse than in the natural kimchi but the ratio of dextran formers were similar. As contrasted with natural kimchi, Leuconostoc was rather increased up to 41.4% and Lactobacillus was decreased down to 29.3%. Dominant species in each genus were Leu. mesenteroides and Lac. minor. Thus it is believed that tyrosine had inhibition effect for the growth of most Lactobacillus in kimchi.
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Effect of Soil Characteristics on VOC Detoxification under Microwave Vacuum Radiation
박현근,전인철 한국환경관리학회 2003 하계학술연구발표회 Vol.2003 No.-
비극성 유기오염물질(Toluene, p-Xylene)로 오염된 토양에 전자기파로 조사할 때 VOC의 제거에 영향을 미치는 토양의 물리적 특성에 관하여 연구하였다. 토양내의 수분은 VOC제거에 크게 영향을 주었으며, 수분함량(0∼10wt.%)에 따라 제거속도가 비례적으로 증가하였고 모래 입자크기는 토양의 유공성으로 인하여 오염물질의 휘발속도에 영향을 주었다. 진흙토양과 모래토양에 전자기파를 조사하였을 때 진흙토양에서 오염물질이 보다 빨리 추출되었다. 그리고 토양속에서 오염물질의 잠복기간에 관계없이 오염물질의 휘발속도는 동일하였다. Various crucial parameters and conditions were investigated to enhance the extraction of nonpolar organic contaminants, toluene and p-xy-lene, from sandy and clay soil during microwave heating. The presence of water(0 to 10.00wt.%) in the soil keeps its dielectric loss factor relatively high at lower temperature and gives impetus to vaporize the organic component, thus enhancing the rate of p-xylene removal. In sandy soil, the soil texture(grain size) altered the dielectric properties of the wet soil due to porosity, which modified the rate of volatilization of the organic chemical. Also clay soil appeared to be more sensitive to microwave radiation than sandy soil, thus yielding a faster extraction rate. The volatilization rates of the organic contaminant from soil were found to be the same, regardless of its incubation period in soil(1 day, 2 and 31 days).
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