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Schwanniomyces castellii CBS 2863(ATCC 26077)으로부터 $\alpha$-Amylase 정제 및 특성
박종천,배석,임선영,이진종,이향,전순배,Park, Jong-Chun,Bai, Suk,Lim, Suhn-Young,Lee, Jin-Jong,Lee, Hyang,Chun, Soon-Bai 한국미생물·생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.6
The extracellular alpha-amylase was purified to homogenity from the culture filtrate of starch grown Sch. castellii CBS 2863. The purified enzyme was glycoprotein with a molecular weight of about 56 kDa. The pH and temperature optimum were 5.5 and 40C, respectively. The enzyme was fairly stable up to 40C and at acid pH range (pH 4.0-7.0). The apparent Km and Vmax of the enzyme toward starch was 1.0mg/ml and 100U/mg protein, respectively. The analysis of amino acid composition was found to be acidic protein. The amino acid sequence of N-terminal peptide consisted of Asp-Val-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr.
Schwanniomyces castellii CBS 2863으로부터 ${\alpha}$-Amylase 유전자 Cloning
박종천,배석,전순배,Park, Jong-Chun,Bai, Suk,Chun, Bai-CHun 한국미생물학회 1994 미생물학회지 Vol.32 No.1
Schwanniomyces castellii의 제놈 DNA로 제조된 유전자 은행으로부터 cloning된 ${\alpha}$-amylase 유전자가 Sacchromyces cerevisiae에서 발현되었다. Cloning된 삽입 DNA 절편의 크기는 약 5.0 kb이었고, Southern 및 immunoblot 분석 결과 cloning된 ${\alpha}$-amylase 유전자가 Sch. Castellii로부터 유래되었음이 확인되었다. S. cerevisiae SHY3 형질전환체에서 Sch. Castellii ${\alpha}$-amylase 유전자발현은 모균주에 비해 낮았으나, 단백질의 분자량 및 효소의 성질은 Sch. Castellii에서 분리한 ${\alpha}$-amylase의 그것과 차이가 없었다. The gene encoding ${\alpha}$-amylase of Schwanniomyces castellii was cloned in Saccharomyces cerevisiae. The 5.0-kilobase insert was shown to direct the synthesis of ${\alpha}$-amylase. Southern blot analysis confirmed that this ${\alpha}$-amylase gene was derived from the genomic DNA of Sch. castellii. Immunoblot analysis showed that ${\alpha}$-amylase production from S. cerevisiae transformant was less than that of donor strain. The ${\alpha}$-amylase secreted from S. cerevisiae transformant was shown to be indistinguishable from that of Sch. castellii on the basis of molecular weight and enzyme properties.
Schwanniomyces occidentalis var. persoonii CBS 2169 $\alpha$-Amylase 유전자의 Nucleotide Sequence
박종천,배석,오상진,이진종,전순배,Park, Jong-Chun,Bai, Suk,Oh, Sang-Jin,Lee, Jin-Jong,Chun, Soon-Bai 한국미생물·생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.6
The relationship between Schwanniomyces occidentalis CBS 2863 (formerly castellii) and CBS 1153 (formerly alluvius), and their variety persoonii was examined at alpha-amylase gene level. Using Sch. occidentalis alpha-amylase gene as probe, Sch. occidentalis alpha-amylase gene homologues were obtained from Sch. occidentalis CBS 1153 and Sch. occidentalis var. persoonii. The restriction analysis of these homologues showed that the restriction enzyme sites between Sch. occidentalis CBS 2863 and CBS 1153 was identical but different between these strains and Sch. occidentalis var. persoonii.
박종천,김진,이우범,이성우,주현수,Park, Jong-Chun,Kim, Jin,Lee, Woo-Bum,Lee, Sung-Woo,Joo, Hyun-Soo 한국하천호수학회 2000 생태와 환경 Vol.33 No.1
본 연구는 광양만 퇴적물의 이화학적 조성과 중금속 함량을 알아보기 위해 함수율, COD, $H_{2}S$, 입도 및 10가지의 중금속을 17개 조사지점에 대해 분석${\cdot}$조사하였다. 조사기간동안 나타난 결과로서 퇴적물의 함수율 변화는 35.5${\sim}$53.8%를 나타내었다. COD와 $H_{2}S$는 각각 3.8${\sim}$12.9 mg/g, 0.1${\sim}$11.4 ${\mu}g/g$를 나타내었다. 퇴적물 입도의 구성은 입경 $74{\mu}m$ 이하가 40.5${\sim}$86.7% 74${\mu}m$ 이상이 11.5${\sim}$43.0%로 나타났다. 등농도 분포도를 이용한 중금속의 공간분포로부터 광양만 퇴적물에 영향을 미치는 몇 곳의 배출원을 추정할 수 있었다. 중금속 배출원 중 여천공단 내의 신풍천, 쌍봉천 그리고 하수처리장의 방류수역이 주요 배출원으로 판정되었다. 중금속의 분석 결과를 US, EPA 비오염해역의 guideline에 비교할 때 납과 수은은 guideline에 모두 적합한 것으로 평가되었으나 망간, 아연, 구리, 철, 비소, 크롬 등은 guideline을 모두 상회하고 있었다. For the purpose of surveying the physicochemical composition of sediment collected from Kwangyang Bay, the percentage of water loss, COD, $H_2S$, grain size and 10 heavy metals were studied at 17 sites. During the surveying period, the changes of the percentage of water loss were appeared $35.5\;{\sim}\;53.8%$. COD and $H_2S$ were showed $3.8\l{\sim}\;12.9\;mg/g$, and $0.1\;{\sim}11.4\;{\mu}g/g$, respectively, In composition of grain size on the sediment, percentages of grain sizes under $74\;{\mu}m$ were varied from 40.5% to 86.7% and above $74\;{\mu}m$ were varied from 11.5% to 43.0%. From the spatial distribution of heavy metal using contour map, we can suppose some heavy metal discharges which affect sediment of Kwangyang Bay, It was estimated that Shinpung creek, Ssang-bong creek, and draining area of sewange treatment plant were the main discharge among the heavy metal output sources. By comparison between present study and heavy metal guideline of nonpolluted sea sediment that is provided by EPA, US, it was showed that the contents of Pb and Hg were acceptable but contents of Mn, Zn, Cu, Fe, As, and Cr were higher than those of EPA guideline.
박종천,이우범,주현수 ( Jong Chun Park,Woo Bum Lee,Hyun Soo Joo ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
In Kwangyang bay, the biological and ecological surveys were carried out at seventeen sites both in surface and bottom water from July 1996 to May 1997. Fifteen abiotic and biotic parameters were determined on each sample. These data were subjected to simple regression, multiple stepwise regression and the principal factor analysis-to scrutinize the effect of environmental conditions on water quality and ecosystem. By the results of these analyses in Kwangyang bay, ecosystem were regulated by five factors for four seasons. The results showed that distribution of the microbial population was mainly influenced by inflow of fresh water, nitrogenous nutrients, suspended solids and water temperature. The overall size of microbial population was greater ten and five times than those obtained in 1982 and 1992, respectively.
특집 : 다산학(茶山學)의 경학적 원류 ; 다산(茶山) 정약용(丁若鏞)의 『독례통고(讀禮通考)』 연구(硏究) 초탐(初探)
박종천 ( Jong Chun Park ) 한국실학학회 2011 한국실학연구 Vol.0 No.21
정약용(丁若鏞, 1762~1836)은 청(淸)나라 서건학(徐乾學, 1631~1694)의 『독례통고(讀禮通考)』를 비평적으로 수용하고 활용함으로써, 조선 후기 예학사에서 청초(淸初) 고증학적 예학의 박학(博學)적 장점과 조선 예학의 실용(實用)적 특장을 잘 결합시켜서 새로운 실학적 예학의 가능성을 선보였다. 특히, 『상의절요(喪儀節要)』의 「오복도표(五服圖表)」는 상기(喪期)를 중심으로 구성된 『독례통고』의 「상기표(喪期表)」의 번잡한 문제점을 극복하기 위해 친족(親族) 중심의 새로운 편제를 시도함으로써 실용성을 높이는 한편, 고례(古禮)의 이상을 반영하는 『의례(儀禮)』를 중심으로 하는 예경(禮經)과 역대 주석과 예제 및 국전(國典) 등을 일목요연하게 비교할 수 있도록 함으로써 학문적인 고증과 심층적인 비교를 위한 박학적 체계성을 합리적 실용성과 접목시켰다. 『예고서정(禮考書頂)』과 『독례통고전주(讀禮通考箋注)』는 『독례통고』를 연구하는 구체적인 과정을 잘 보여주었고, 『상례사전(喪禮四箋)』은 체계와 구성방식에서 『독례통고』의 영향을 적극적으로 수용해서 발전시켰으며, 『풍수집의(風水集議)』는 『독례통고』를 통해 유교적 풍수비판을 집대성함으로써 산송(山訟)을 비롯한 각종 사회적 문제를 일으키는 풍수(風水)에 대한 이론적이고 체계적인 비평으로서 주목할 만하다. 이러한 작업을 통해 정약용은 조선 후기 『독례통고』연구의 최고봉을 이루게 된다. Yak-yong Cheong(丁若鏞, 1762~1836) is the most brilliant ritual theorist that combined practical ritual studies in the Chosun(朝鮮) dynasty and erudite ritual studies in the Ching(淸) dynasty. He critically studied Qianxue Xu(徐乾學, 1631~1694)`s Dulitongkao(讀禮通考). In Sangryesajeon(喪禮四箋), Cheong had accepted the format of Dulitongkao and developed it systematically. Sanguijeolyo (喪儀節要) reconstructed some important charts and diagrams of Dulitongkao rationally and practically. Yegoseojeong(禮考書頂) and Tokryetonggojeonju(讀禮通考箋注) showed his study process and situation of Dulitongkao obviously. Pungsujipui(風水集議) represented his critical ritual studies of Fengshui(風水).
2차원 진행규칙파열에서의 쇄파현상에 따른 비선형성 파의 특성에 관한 수치적 연구
박종천(Jong-Chun Park),宮田 秀明(Hideaki Miyata) 한국해양공학회 1996 韓國海洋工學會誌 Vol.10 No.3
The breaking phenomenon of regular periodic waves generated by a numerical wave maker is simulated by finite-difference method which can cope with strong interface motions. The air and water flows are simultaneously solved in the time-marching solution procedure for the Navier-Stokes equation. A density-function technique is devised for the implementation of the interface conditions. The accuracy is examined and applied to the simulation of two-dimensional breaking phenomena of periodic gravity waves.