Schwanniomyces castellii CBS 2863으로부터 ${\alpha}$-Amylase 유전자 Cloning

박종천,배석,전순배,Park, Jong-Chun,Bai, Suk,Chun, Bai-CHun 한국미생물학회 1994 미생물학회지 Vol.32 No.1

Schwanniomyces castellii의 제놈 DNA로 제조된 유전자 은행으로부터 cloning된 ${\alpha}$-amylase 유전자가 Sacchromyces cerevisiae에서 발현되었다. Cloning된 삽입 DNA 절편의 크기는 약 5.0 kb이었고, Southern 및 immunoblot 분석 결과 cloning된 ${\alpha}$-amylase 유전자가 Sch. Castellii로부터 유래되었음이 확인되었다. S. cerevisiae SHY3 형질전환체에서 Sch. Castellii ${\alpha}$-amylase 유전자발현은 모균주에 비해 낮았으나, 단백질의 분자량 및 효소의 성질은 Sch. Castellii에서 분리한 ${\alpha}$-amylase의 그것과 차이가 없었다. The gene encoding ${\alpha}$-amylase of Schwanniomyces castellii was cloned in Saccharomyces cerevisiae. The 5.0-kilobase insert was shown to direct the synthesis of ${\alpha}$-amylase. Southern blot analysis confirmed that this ${\alpha}$-amylase gene was derived from the genomic DNA of Sch. castellii. Immunoblot analysis showed that ${\alpha}$-amylase production from S. cerevisiae transformant was less than that of donor strain. The ${\alpha}$-amylase secreted from S. cerevisiae transformant was shown to be indistinguishable from that of Sch. castellii on the basis of molecular weight and enzyme properties.

Schwanniomyces occidentalis var. persoonii CBS 2169 $\alpha$-Amylase 유전자의 Nucleotide Sequence

박종천,배석,오상진,이진종,전순배,Park, Jong-Chun,Bai, Suk,Oh, Sang-Jin,Lee, Jin-Jong,Chun, Soon-Bai 한국미생물·생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.6

The relationship between Schwanniomyces occidentalis CBS 2863 (formerly castellii) and CBS 1153 (formerly alluvius), and their variety persoonii was examined at alpha-amylase gene level. Using Sch. occidentalis alpha-amylase gene as probe, Sch. occidentalis alpha-amylase gene homologues were obtained from Sch. occidentalis CBS 1153 and Sch. occidentalis var. persoonii. The restriction analysis of these homologues showed that the restriction enzyme sites between Sch. occidentalis CBS 2863 and CBS 1153 was identical but different between these strains and Sch. occidentalis var. persoonii.

Schwanniomyces castellii CBS 2863(ATCC 26077)으로부터 $\alpha$-Amylase 정제 및 특성

박종천,배석,임선영,이진종,이향,전순배,Park, Jong-Chun,Bai, Suk,Lim, Suhn-Young,Lee, Jin-Jong,Lee, Hyang,Chun, Soon-Bai 한국미생물·생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.6

The extracellular alpha-amylase was purified to homogenity from the culture filtrate of starch grown Sch. castellii CBS 2863. The purified enzyme was glycoprotein with a molecular weight of about 56 kDa. The pH and temperature optimum were 5.5 and 40C, respectively. The enzyme was fairly stable up to 40C and at acid pH range (pH 4.0-7.0). The apparent Km and Vmax of the enzyme toward starch was 1.0mg/ml and 100U/mg protein, respectively. The analysis of amino acid composition was found to be acidic protein. The amino acid sequence of N-terminal peptide consisted of Asp-Val-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr.

보문 : Polyethylene Film포장이 포도 Sheridan의 저장력에 미치는 영향

남상영(Sang Young Nam),김경미(Kyoung Mi Kim),박종천(Jong Chun Park),주선종(Seon Jong Joo),정재현(Jai Hyun Jung) 한국식품저장유통학회(구 한국농산물저장유통학회) 1997 한국식품저장유통학회지 Vol.4 No.1

광양만의 퇴적물에 대한 이화학적 조성 및 중금속 함량

박종천,김진,이우범,이성우,주현수,Park, Jong-Chun,Kim, Jin,Lee, Woo-Bum,Lee, Sung-Woo,Joo, Hyun-Soo 한국하천호수학회 2000 생태와 환경 Vol.33 No.1

본 연구는 광양만 퇴적물의 이화학적 조성과 중금속 함량을 알아보기 위해 함수율, COD, $H_{2}S$, 입도 및 10가지의 중금속을 17개 조사지점에 대해 분석${\cdot}$조사하였다. 조사기간동안 나타난 결과로서 퇴적물의 함수율 변화는 35.5${\sim}$53.8%를 나타내었다. COD와 $H_{2}S$는 각각 3.8${\sim}$12.9 mg/g, 0.1${\sim}$11.4 ${\mu}g/g$를 나타내었다. 퇴적물 입도의 구성은 입경 $74{\mu}m$ 이하가 40.5${\sim}$86.7% 74${\mu}m$ 이상이 11.5${\sim}$43.0%로 나타났다. 등농도 분포도를 이용한 중금속의 공간분포로부터 광양만 퇴적물에 영향을 미치는 몇 곳의 배출원을 추정할 수 있었다. 중금속 배출원 중 여천공단 내의 신풍천, 쌍봉천 그리고 하수처리장의 방류수역이 주요 배출원으로 판정되었다. 중금속의 분석 결과를 US, EPA 비오염해역의 guideline에 비교할 때 납과 수은은 guideline에 모두 적합한 것으로 평가되었으나 망간, 아연, 구리, 철, 비소, 크롬 등은 guideline을 모두 상회하고 있었다. For the purpose of surveying the physicochemical composition of sediment collected from Kwangyang Bay, the percentage of water loss, COD, $H_2S$, grain size and 10 heavy metals were studied at 17 sites. During the surveying period, the changes of the percentage of water loss were appeared $35.5\;{\sim}\;53.8%$. COD and $H_2S$ were showed $3.8\l{\sim}\;12.9\;mg/g$, and $0.1\;{\sim}11.4\;{\mu}g/g$, respectively, In composition of grain size on the sediment, percentages of grain sizes under $74\;{\mu}m$ were varied from 40.5% to 86.7% and above $74\;{\mu}m$ were varied from 11.5% to 43.0%. From the spatial distribution of heavy metal using contour map, we can suppose some heavy metal discharges which affect sediment of Kwangyang Bay, It was estimated that Shinpung creek, Ssang-bong creek, and draining area of sewange treatment plant were the main discharge among the heavy metal output sources. By comparison between present study and heavy metal guideline of nonpolluted sea sediment that is provided by EPA, US, it was showed that the contents of Pb and Hg were acceptable but contents of Mn, Zn, Cu, Fe, As, and Cr were higher than those of EPA guideline.

광양만 생태계의 미생물군집에 관한 환경요인 분석

박종천,이우범,주현수 ( Jong Chun Park,Woo Bum Lee,Hyun Soo Joo ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

In Kwangyang bay, the biological and ecological surveys were carried out at seventeen sites both in surface and bottom water from July 1996 to May 1997. Fifteen abiotic and biotic parameters were determined on each sample. These data were subjected to simple regression, multiple stepwise regression and the principal factor analysis-to scrutinize the effect of environmental conditions on water quality and ecosystem. By the results of these analyses in Kwangyang bay, ecosystem were regulated by five factors for four seasons. The results showed that distribution of the microbial population was mainly influenced by inflow of fresh water, nitrogenous nutrients, suspended solids and water temperature. The overall size of microbial population was greater ten and five times than those obtained in 1982 and 1992, respectively.

19-20C 청도 밀양박씨의 향약과 계

박종천 ( Park Jong-chun ) 서울대학교 종교문제연구소 2018 종교와 문화 Vol.0 No.34

The community compacts and mutual aid societies of Milyang Park family in the region of Cheongdo in the 19th and 20th Centuries had showed as follows: (1) The promotions of learning and educational activities of local aristocrats in Joseon dynasty based on the foundation of confucian norms represented how to take control of cultural hegemony in the local area. (2) The community compacts and mutual aid societies of Milyang Park family in the region of Cheongdo built social safety net. And they expended educational activities confined within the relatives, and thereby strengthened the social status of Milyang Park family in the regional community in the 19th through 20th centuries. (3) Janghyun Park inherited the tradition of his family, and thereby developed confucian tradition into national confucianism in modern Korea.

삼위일체와 삼교회통

박종천(Jong-Chun Park) 감리교신학대학교 2000 신학과세계 Vol.- No.41

『민보의(民堡議)』 필사본에 대한 연구

박종천 ( Jong Chun Park ) 다산학술문화재단 2008 다산학 Vol.- No.12

Tasan`s Minboui (1812) is the most pioneering and influential military book concerning the Civil Defense Fortifications in late Joseon dynasty. In this article, I attempt to study the classification and characteristics of Minboui manuscripts through a textual critique. My arguments are as follows: 1. The manuscript of Minboui in Addendum to Complete Writings of Yeoyudang (與猶堂全書補遺: ACWY) is the original version of all existing manuscripts of Minboui in the Yeoyudangjip (與猶堂集: YJ) series. 2. Manuscripts of Minboui in the YJ series are the prototypes of non-YJ versions. The latter versions which have the table of contents of Minboui (民堡議目次: TC-M) are arbitrary variations of the former versions which do not contain TC-M. 3. All manuscripts of Minboui in the YJ series is classified into Saam byeoljip (俟菴別集: SBJ) versions with TC-M of the first volume and the □ volume and non-SBJ versions with TC-M of the first volume and the last volume. SBJ versions are better than non-SBJ versions. 4. SBJ versions are classified into two types of manuscripts: SBJ-A version is the Berkeley manuscript which is the revised version of the ACWY manuscript. SBJ-B versions are the manuscript of Minboui in the Kyujanggak Collection (奎章閣本: KC) and the manuscript in A Complete Collection of Cheong Tasan (丁茶山全書: CCCT). 5. SBJ-A versions are the preceding manuscripts of SBJ-B versions. Therefore, the critical text of Minboui in A Formal Edition of Complete Writings of Yeoyudang (定本與猶堂全書: FECWY) published in the future shall be composed on the basis of a comparative textual critique of SBJ versions which are the best versions of all manuscripts of Minboui.

특집 : 다산학(茶山學)의 경학적 원류 ; 다산(茶山) 정약용(丁若鏞)의 『독례통고(讀禮通考)』 연구(硏究) 초탐(初探)

박종천 ( Jong Chun Park ) 한국실학학회 2011 한국실학연구 Vol.0 No.21

정약용(丁若鏞, 1762~1836)은 청(淸)나라 서건학(徐乾學, 1631~1694)의 『독례통고(讀禮通考)』를 비평적으로 수용하고 활용함으로써, 조선 후기 예학사에서 청초(淸初) 고증학적 예학의 박학(博學)적 장점과 조선 예학의 실용(實用)적 특장을 잘 결합시켜서 새로운 실학적 예학의 가능성을 선보였다. 특히, 『상의절요(喪儀節要)』의 「오복도표(五服圖表)」는 상기(喪期)를 중심으로 구성된 『독례통고』의 「상기표(喪期表)」의 번잡한 문제점을 극복하기 위해 친족(親族) 중심의 새로운 편제를 시도함으로써 실용성을 높이는 한편, 고례(古禮)의 이상을 반영하는 『의례(儀禮)』를 중심으로 하는 예경(禮經)과 역대 주석과 예제 및 국전(國典) 등을 일목요연하게 비교할 수 있도록 함으로써 학문적인 고증과 심층적인 비교를 위한 박학적 체계성을 합리적 실용성과 접목시켰다. 『예고서정(禮考書頂)』과 『독례통고전주(讀禮通考箋注)』는 『독례통고』를 연구하는 구체적인 과정을 잘 보여주었고, 『상례사전(喪禮四箋)』은 체계와 구성방식에서 『독례통고』의 영향을 적극적으로 수용해서 발전시켰으며, 『풍수집의(風水集議)』는 『독례통고』를 통해 유교적 풍수비판을 집대성함으로써 산송(山訟)을 비롯한 각종 사회적 문제를 일으키는 풍수(風水)에 대한 이론적이고 체계적인 비평으로서 주목할 만하다. 이러한 작업을 통해 정약용은 조선 후기 『독례통고』연구의 최고봉을 이루게 된다. Yak-yong Cheong(丁若鏞, 1762~1836) is the most brilliant ritual theorist that combined practical ritual studies in the Chosun(朝鮮) dynasty and erudite ritual studies in the Ching(淸) dynasty. He critically studied Qianxue Xu(徐乾學, 1631~1694)`s Dulitongkao(讀禮通考). In Sangryesajeon(喪禮四箋), Cheong had accepted the format of Dulitongkao and developed it systematically. Sanguijeolyo (喪儀節要) reconstructed some important charts and diagrams of Dulitongkao rationally and practically. Yegoseojeong(禮考書頂) and Tokryetonggojeonju(讀禮通考箋注) showed his study process and situation of Dulitongkao obviously. Pungsujipui(風水集議) represented his critical ritual studies of Fengshui(風水).