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박경의,김정구,이동옥,김훈,구승엽,김석현,최영민 대한골다공증학회 2012 Osteoporosis and Sarcopenia Vol.10 No.3
Objectives: To investigate the relationship between single nucleotide polymorphism (SNP)s in Wnt antagonist genes, and production of osteoprotegerin (OPG) and soluble receptor activator of NF-κB ligand (sRANKL) by whole blood cells after hormone therapy (HT) in postmenopausal Korean women. Materials and Methods: The Dkk1 c.318A>G, Dkk2 c.437G>A, Dkk3 c.1003A>G polymorphisms and sFRP3 c.970C>G, sFRP4 c.958C>A, and c.1019G>A polymorphisms, and sFRP5 c.20G>C polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), direct sequencing, and Taqman assay in 75 postmenopausal Korean women receiving estrogen-progestogen therapy. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells (WBC) before and after HT of 6 months were also measured. Results: Changes in the production of OPG and sRANKL by lipopolysaccharide-stimulated WBC, and in ratios of sRANKLx1,000/OPG after HT of 6 months were not different according to SNPs in Wnt signal pathway genes except Dkk1 c.318A>G SNP. The AA genotype of Dkk1 c.318A>G SNP showed significantly higher changes (p<0.05) in ratios of sRANKLx1,000/OPG compared to other genotypes. There were no significant differences in changes in the production of OPG and sRANKL, and in ratios of sRANKLx1,000/OPG among combined genotypes of sFRP4 c.958C>A, and c.1019G>A polymorphisms after HT. Conclusions: Dkk1 c.318A>G SNP are related with changes in ratios of sRANKLx1,000/OPG in terms of the production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells after HT.
The Use of Tenaculum During Intrauterine Insemination May Not Affect the Pregnancy Outcome
박경의,김훈,지병철,서창석,김석현,최영민,김정구,문신용,구승엽 연세대학교의과대학 2010 Yonsei medical journal Vol.51 No.6
Purpose: The association between tenaculum application to the cervix just before embryo transfer and lower pregnancy rate has been reported. However, studies on the use of tenaculum in intrauterine insemination (IUI) cycles are not available. This study attempted to investigate whether the use of tenaculum affects the clinical outcomes of IUI. Materials and Methods: Two hundred and thirty three CC/hMG/IUI cycles of one hundred and forty three couples were recruited at Seoul National University Hospital from October 2006 to December 2008. Mock insemination and IUI with or without tenaculum application to the cervix were also performed, and clinical pregnancy rate was compared. Results: The incidence of difficult mock insemination at the beginning of cycle was higher in the tenaculum use group (p <0.001). No significant statistical difference in the clinical pregnancy rate was observed between the groups with or without tenaculum application (12.1% vs. 18.5%; p =0.175), which was not influenced by the difficulty of mock insemination. Conclusion:The use of tenaculum during IUI may not affect the pregnancy outcome. Our results need to be confirmed by a prospective study in a larger population.
김용진,박경의,김윤영,김훈,구승엽,서창석,김석현,최영민 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.1
The preservation of female germ cells is important in the individuals with ovarian dysfunction and failure. For this purpose, ovarian follicle in vitro maturation (OFIVM) is an important technology for the retrieval of mature oocytes. In the in vivo follicular development, paracrine factors such as angiotensin (AT) and anti-Mu¨llerian hormone (AMH) play important roles. We attempted to add estrogen during the OFIVM and to assess their expression on the follicular cells. The ovaries and pre-antral follicles were collected from 13-day C57BL/6 mice and cultured in vitro with estradiol (E2) treatment for up to two weeks. In the whole ovaries, the expression of AT II was decreased and the expression of AMH was similar between control and E2-treated ovaries after in vitro culture. Although there was no difference in the survival, ovulation, maturation and fertilization rates between control and E2-treated groups, the expression of AT II in the follicular cells was down-regulated after E2 treatment at mRNA level, and AMH showed similar expression. In conclusion, adding E2 in OFIVM may regulate paracrine factors and their receptors that are related to follicular development. Further investigations are necessary to elucidate the roles of various sex hormones in the regulation of AT and AMH expression during the OFIVM.
김중현,정은희,이경의,남춘희,박성애,박찬호,남기흠,이병윤,서민환 한국식물분류학회 2016 식물 분류학회지 Vol.46 No.1
연구는 욕지도의 관속식물상과 식생을 밝히기 위하여 2015년 3월부터 2016년 1월까지 총 6회에 걸쳐 21일간 현지조사를 실시하였다. 관속식물은 113과 354속 487종 4아종 50변종 4품종 2교잡종의 총 547 분류군으로 정리되었다. 이 중 기존의 조사에서는 발견되지 않았으나 본 연구에서 처음으로 밝혀진 분류군 은 314분류군이었다. 한반도 고유종 8분류군, IUCN 평가기준에 따른 적색목록식물 6분류군, 식물구계학적 특정식물 86분류군이 조사되었다. 본 조사지에서 다른 지역에 비해 특이적으로 분포하는 식물은 99분류군이 며, 이 중 남방계식물은 97분류군, 제한분포 식물은 2분류군이 확인되었다. 귀화식물은 74분류군이 발견되었다. 욕지도의 산림식생은 곰솔군락과 사방오리군락이 주를 이루는 것으로 나타났다. 군락의 수직구조는 안정 적이었으며, DBH-class 분석 결과 교목층의 우점종이 유지될 것으로 나타났다. 간벌과 같은 교란인자가 제거 되면 초본과 목본종의 구성은 현재와 다른 양상이 나타날 것으로 보인다. This study was carried out to investigate the flora and vegetation of Yokjido Island in Tongyeong-si, Korea. Vascular plants collected six times (from March of 2015 to January of 2016) were identified as 547 taxa in total, includ- ing 113 families, 354 genera, 487 species, four subspecies, 50 varieties, four forms and two hybrids. The first records from this region contained 314 taxa. In the flora of this area, endemic Korean species numbered eight taxa, and the red list of vascular plants according to the IUCN valuation basis numbered six taxa. In addition, 86 taxa of floristic regional indicator plants specially designated by the Ministry of Environment are included. Among the species investigated in this survey, 99 taxa were identified as specific species biogeographically as compared floras of other terrestrial regions; 97 taxa of plants in southern areas and two taxa of limited distribution plants on the Korean Peninsula. In all, 74 naturalized plants were recorded in the investigated area. Samples of the forest vegetation of Yokjido Island were mainly classified as Pinus thunbergii community and the Alus firma community. Vertical structures of the communities were stable, and a DBH-class analysis showed that the dominant tree species would be maintained. If disturbance factors such as thinning could be halted, the composition of woody species and herbaceous species would change.
The Expression Profile of Angiotensin System on Thawed Murine Ovaries
김윤영,김용진,조경미,김수현,박경의,강병철,정경천,김문석,구승엽 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.6
Freezing and thawing is one of the most widely used tissue engineering techniques for the preservation of ovaries. Many cells and tissues demonstrate changes in functional gene expression after thawing. Several studies have reported the important roles of angiotensin (AT) system during the ovarian follicular growth. AT system consists of ATII, and ATII receptors type I (ATII-RI) and type II (ATII-RII). However, little is known whether frozen-thawed ovaries show any alteration of AT system member gene expression when treated with survival-enhancing factors. We aimed to investigate whether mass freezing and thawing with or without the use of Rho-associated kinase (ROCK) inhibitors up- or down-regulate the expression of ATII, ATII-RI, and ATII-RII genes on frozen-thawed ovarian tissues. Significant changes in the expression of ATII, ATII-RI, and ATII-RII genes were observed on thawed ovaries when compared to fresh control. The treatment with ROCK inhibitors did not significantly alter their expression. In conclusion, freezing and thawing of ovarian tissue may affect the mRNA expression levels of intra-ovarian AT system genes, and modulation of ROCK inhibitor activity may not regulate AT system on the frozenthawed ovarian tissue.
소형 전자 기기의 열설계를 위한 복합층 방열시트의 등가 열물성 측정법
이요셉(J.S. Lee),박경의(K.U. Park),김동현(D.H. Kim),임영태(Y.T. Im),김인중(I.J. Kim),안원기(W.K. Ahn),김민수(M.S. Kim),홍석호(S.H. Hong) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.11
Portable electronic devices generate heat more and more because of high power and thinner design specifications. The excessive heat makes the devices operate not properly and makes customers feel uncomfortable. To dissipate such excessive heat, various multi-layer heat spreading sheets (HSS) are attached to the various components of electronic devices. This study is conducted for the optimized thermal design of portable devices in the aspect of an analytical simulation modeling of HSS. First of all, test module which is similar to the product was employed and the test was done following the Graphite sheet. This result is used as input data for the computer simulation to calculate the equivalent thermal properties. Then the analytical result is compared and verified with theoretical thermal properties. Finally, thermal properties which can be applied to the concept design stage have been proposed.