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여성 생삭기에 있어서의 ${\beta}$-Endorphin에 관한 면역조직학적 연구
김정구,민응기,문신용,이진용,장윤석,Kim, Jung-Gu,Min, Eung-Gi,Moon, Shin-Yong,Lee, Jin-Yong,Chang, Yoon-Seok 대한생식의학회 1988 Clinical and Experimental Reproductive Medicine Vol.15 No.1
The aim of this study was to examine the presence of ${\beta}$-endorphin in female reproductive organs. A total of 104 fresh tissue samples were obtained from normal ovary, tube, endometrium, placenta, amniotic membrane and umbilical cord, and immunostained by the method using biotin-streptoavidin amplified system. The results were as follows: 1. In reproductive age, corpus luteum only showed ${\beta}$-endorphin immunostained cells but no cells in ovaries during proliferative phase of menstrual cycle were stained. 2. Secretory endometrium revealed positive reactions in the cytoplasm of glandular epithelial cells and around the vessels, while proliferative endometrium negative reactions. 3. All the tissues of menopausal women were negative to ${\beta}$-endorphin antibody. 4. In the pregnant women, there are no ${\beta}$-endorphin containing cells in the placenta, amniotic membrane and umbilical cord regardless of gestational age.
치자중 호기성 세균에 의해 청색색소로 변환되는 성분의 단리
박창훈,강소임,민응기,한영환,이정규,이동웅,Park, Chang-Hun,Kang, So-Im,Min, Eung-Gi,Han, Yeong-Hwan,Lee, Chung-Kyu,Lee, Dong-Ung 한국생약학회 1998 생약학회지 Vol.29 No.3
Geniposide, an iridoid glucoside, has been isolated from the butanol fraction of Gardenia jasminoides Ellis (Rubiaceae). The component was found to be transformed into the blue pigments by some aerobic bacteria, suggesting that geniposide is the precursor for the formation of pigments after converting into genipin, an aglycon of geniposide, by ${\beta}-glucosidase$. Some bacteria having a ${\beta}-glucosidase$ activity did not form the pigments, which may mean that the formation of pigments can only be occurred by the reaction of any enzyme or compound in the pigment-producing bacteria.
김충현,정경순,박소현,황도영,김기철,민응기,Kim, Chung-Hyon,Cheong, Kyung-Soon,Park, So-Hyun,Hwang, Do-Yeong,Kim, Ki-Chul,Min, Eung-Gi 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1
To confirm the overcome of in vitro 2-cell block, ICR mouse I-cell embryos were cultured in CZB media. All embryos in CZB were overcome in vitro 2-cell block and 92% of embryos were developed to the blastocyst at day 4. However, in m-KRB group(control) only 20% of embryos were developed over 2-cell. Any embryos in m-KRB did not develop to the morular stage. Developments and degenerations of ICR mouse I-cell embryos were compared in CZB medium prepared with water of three quality:(l) Milli-Q ultrafiltration water(UF);(2) Milli-Q reverse osmosis water(RO);(3) tap water(TAP). The objective was to evaluate the potential of quality control using ICR mouse 1-cell embryos. The more water was purified, the better embryo developments were supported and the less embryos were degenerated. As a quality control system, the culture of ICR 1-cell mouse embryos in CZB was useful.
김충현,장은주,정경순,박소현,황도영,김기철,민응기,Kim, Chung-Hyon,Chang, Eun-Ju,Cheong, Kyung-Soon,Park, So-Hyun,Hwang, Do-Yeong,Kim, Ki-Chul,Min, Eung-Gi The Korean Society for Reproductive Medicine 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.3
난구세포는 lactate와 pyruvate를 쉽게 생성하고, 이로 인해 배양액내 에너지원의 농도를 변화시켜 난자의 수정과 배양에 영향을 주는 것으로 알려져 있다. Glucose, lactate 및 pyruvate의 농도가 상이한 M16, MTF 및 CZB배양액에서 난구세포를 포함한 생쥐난자의 체외 수정과 발달을 관찰하여, 이들 기질의 영향에 대하여 살펴보고 배양액의 유용성을 재검토하고자 하였다. Glucose를 제거한 배양액 (CZ2 배양액)에서 수정율과 배반포 형성율은 다른 배양액에 비해 유의하게 감소되었으나 (p<0.05), 생쥐 난관액과 동일한 기질 농도로 조성된 MTl (난간액이 난구세포를 포함하고 있을 때) 및 MT2배양액 (난관액이 난구세포를 포함하고 있지 않을 때)과 glucose를 포함한 modified CZB배양액에서는 영향이 없었다. 이와 같은 결과로 기질의 농도를 생리적 수준으로 조정한 배양액의 이용은 난구세포를 포함한 생쥐 난자의 체외수정과 그 발달을 향상시키지 못하고, glucose의 제거는 악영향을 나타내는 것으로 사료된다. Cumulus cells have possibly influence on fertilization of mouse oocytes and their subsequent development in vitro, because they readily produce lactate and pyruvate and can modify the concentration of substrates in the medium. In vitro fertilization of mouse oocytes with cumulus mass and their developments in five media which were differently composed in concentrations of glucose, lactate and pyruvate were observed. In the absence of glucose (CZ2 medium) decreased (p<0.01) the percentage of fertilization and embryos reaching the blastocyst stage. But, in the same concentration of glucose, lactate and pyruvate as mouse oviductal fluid with (MT1 medium) and without (MT2 medium) cumulus mass and modified CZB medium containing glucose (CZ1 medium) had no effects (p>0.05). These studies indicate that the adjustments of energy substrates concentration to the physiological level did not improve the fertilization of mouse oocytes with cumulus mass and their development in vitro, and the deletion of glucose showed adverse effects.
난자공여시술의 결과에 영향을 미치는 임상적 인자에 관한 연구
윤상호 ( Sang Ho Yoon ),김진주 ( Jin Ju Kim ),민응기 ( Eung Gi Min ),김석현 ( Seok Hyun Kim ),최영민 ( Young Min Choi ),김정구 ( Jung Gu Kim ),문신용 ( Shin Yong Moon ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.9
목적: 난자공여시술에서 공여자의 연령을 포함한 난자공여시술의 결과에 영향을 미칠 수 있는 임상적 인자들에 대하여 알아보고, 또한 자궁내막수용성 평가에 이용되고 있는 자궁내막 두께와 내막형태의 초음파 소견이 임신의 예측가능지표로서 의미를 가질 수 있는지 살펴보고자 하였다. 방법: 1992년 3월부터 2004년 2월까지 서울대학교병원 불임클리닉에서 난자공여시술을 시행받은 85명의 환자, 109주기를 대상으로 연구를 시행하였다. 임신군과 비임신군, 공여자의 연령, 수혜자의 연령, 자궁내막 두께 및 형태에 따른 임상적인 특징들을 후향적으로 비교하였으며, 통계적인 처리는 Student t-test와 One way ANOVA 그리고 Chi-square test를 시행하였다. 결과: 임상적 임신율은 주기당 38.5% (42/109), 환자당 48.2% (41/85)였다. 임신군과 비임신군 사이에 수혜자의 연령이나 자궁내막의 두께 및 형태, hCG 투여일의 혈중 에스트라디올 농도, 획득된 난자의 개수, 수정률, 누적배아지수, 수혜자의 체질량지수등은 통계적으로 유의한 차이가 없었으나, 공여자의 나이에 유의한 차이가 있었다 (30.2±3.6 vs 32.1±4.3, P=0.017). 공여자의 나이에 따라 대상군을 세분하여 분석해보면 hCG 투여일의 혈중 에스트라디올 농도, 획득된 난자의 개수와 수정률은 35세 이상군에서 유의하게 낮았으며, 임상적 임신율은 30세 미만군에서 35세 이상군에 비해 유의하게 높았다 (50.0% vs 18.2%, P=0.015). 또한 자궁내막 두께에 따른 임신율은 <7 mm군, 7~9 mm군, 10~13 mm군, ≥14 mm군에서 각각 50.0%, 38.1%, 42.9%, 11.1%였으며, 자궁내막 형태에 따른 임신율은 삼중층구조군과 비삼중층구조군에서 각각 41.6%, 31.3%로 나타나, 자궁내막 두께와 형태 모두 임신율에 유의한 차이는 없었다. 결론: 난자공여시술에서 임신을 예측할 수 있는 가장 신뢰할만한 인자는 공여자의 연령이었고, hCG 투여일에 질식초음파로 측정한 자궁내막의 두께나 삼중층 구조는 임신가능성의 예측지표로서 의미를 가지지 못하는 것으로 나타났다. 수혜자의 연령이나 누적배아지수 또한 임신율에 유의한 영향을 미치지는 않는 것으로 나타났다. Objective: Oocyte donation cycle has been a useful model for the assessment of potential factors affecting human pregnancy, such as uterine receptivity or oocyte quality. The purpose of this study was to investigate variable clinical factors affecting the outcomes of oocyte donation cycles. Methods: This study reviewed 109 cycles of 85 women who underwent oocyte donation in SNUH infertility clinic from March 1992 to February 2004. Variable clinical characteristics were compared between pregnant and non-pregnant group. Data was evaluated by student`s t-test, oneway ANOVA, and Chi-square test. Results: Clinical pregnancy rate was 38.5% per cycle and 48.2% per recipient. When pregnant and non-pregnant groups were compared, there was a significant difference in donor age between both groups. (30.2±3.6 vs. 32.1±4.3, P=0.017). On the other hand, there were no significant differences in mean age, BMI, gravidity of recipient, and peak estradiol level of donor. The number of oocytes retrieved, embryos transferred, fertilization rate, and cumulative embryo score were not different between pregnant and non-pregnant group. Among the various donor age groups, clinical pregnancy rate was significantly higher in <30 years group than ≥35 years (50.0% vs 18.2%, P= 0.015). There were no significant differences for both endometrial thickness and pattern in the pregnancy rate during the IVF-ET cycles by ovum donation. Conclusion: The most reliable predictive factor for pregnancy in oocyte donation cycles is the age of oocyte donor. The mid-cycle endometrial thickness and trilaminar patterns are insignificant predictors. The age of recipient and cumulative embryo score are also insignificant factors.