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진정이용 ( 眞正利用 ) 광물질 측정방법에 의한 칼슘이용성 측정에 관한 연구 1 . 산란 메추리의 칼슘요구량 측정
명규호,선상수,김용식 ( K . H . Myung,S . S . Sun,Y . S . Kim ) 한국축산학회 1983 한국축산학회지 Vol.25 No.6
This experiment was conducted to determine the calcium requirement in the determination of calcium availability. Forty breeding Japanese quails of 13 weeks of age were randomly alloted to 10 calcium input levels(0.240,400,480,560,640,720,800,1000 and 1200㎎) with four replicates of one bird each. After the calcium fasting period of 24hr, the calcium unfed group (Omg) was fed the experimental diet to determine the metabolic fecal calcium (FmCa) plus endogeneous urinary calcium (UeCa), however, the calcium fed groups were forcedly fed the known amounts of calcium into the crop and fed the experimental diet afterward for 48hours. The results of the present study were summarized as follows. 1. According to the results of performance during experimental period, body weight, calcium devoid diet intake and excreta weight of tested birds were not significantly affected by the calcium input level. 2. The relationship between calcium excreta and input of 0 to 400㎎ was not showed, however, the highly correlation (P$lt;0.01) was found between calcium excreta and input of 480 to 1200㎎. The correlation equation between calcium excreta and input of 480 to 1200㎎ was Y = 0.9311 X - 463.6012 (r=0.9929**). 3. The maintenance requirement(FmCa+UeCa) of calcium was 0.71㎎ and production plus repletion requirement of that was 497.9 ㎎ for breeding quails. Therefore, the total calcium requirement of a breeding quail was 498.6㎎ in the determination of calcium availability. In conclusion, it could be said that a 72hours experimental period did not affect to body weight, calcium devoided diet intake and excreta weight of tested birds, and that the total requirement was 498.6㎎ in the determination of calcium availability.
한우와 홀스타인 비육우의 근육 위성세포 Cloning 및 배양
명규호,선상수 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.6
The objectives of this study were to exam optimum culture condition and to compare growth rate of muscle satellite cells derived from Hanwoo and Holstein cattle. Animal growth depends on the proliferation and differentiation of muscle satellite cells. Muscle satellite cells were separated by pronase digestion from 250g muscle and cloned pure cell lines. The optimum cell culture condition was tested with three different culture medium (DME / F12, DMEM, McCoy's 5A) and three different source of serums (fetal calf serum, chicken serum, horse serum). The number of nuclei per unit area was lower in DME/F12 (68%) and McCoy's 5A (64%) than that of DMEM (100%) (P$lt;0.05). Also cell growth rate was lower in CS(37.3%) and HS (10.2 %) than that in FCS (P$lt;0.05). Therefore. the optimum muscle satellite cell culture condition was DMEM + 10 % FCS in both Hanwoo and Holstein cattle.
브로일러 병아리의 아미노산 요구량에 관한연구 1 . 필수아미노산의 적정 급여비율 결정
명규호,한인규,박종만,나현채 ( K . H . Myung,I . K . Han,C . M . Park,H . C . Na ) 한국축산학회 1985 한국축산학회지 Vol.27 No.5
A series of experiments was conducted to determine how the essential amino acids in a corn-soybean diet should be proportioned for maximum growth during the second week posthatching. The diet contained 23% protein made up to 11.15% contributed by conventional ingredients and 11.85% protein in the form of free amino acids. Once the level of an amino acid giving maximum response was established, this level was used in all subsequent experiments. Most amino acids found to be required were lower than the levels of the corresponding amino acids in the starting diet formulated to meet the requirements of NRC (1977). The results indicated that growing broiler chicks require not more than 1.24% argine, 1.30% glycine plus serine, 0.80% isoleucine, 1.10% lysine, 0.40% methionine, 0.63% phenylalanine, 0.72% threonine, 0.17% tryptophan and 0.99% valine.
명규호,박종만,한인규 ( Kyu H . Myung,C . M . Park,In K . Han ) 한국축산학회 1980 한국축산학회지 Vol.22 No.5
This experiment was carried out to investigate appropriatencess in application of NRC (1977) protein requirement for broiler chick in Korea. Growth rate, feed efficiency, nutrient utilizability and feed cost affected by dietary protein levels(23-21%, 11-19%, 19-17% and 17-15% respectively in starting and finishing period) and sexes were examined. Results obtained from the present study can be summarized as follows: 1. It was found that growth rate was significantly improved (P$lt;0.01) as the dietary protein level was increased. Significant differences in growth rates of both male and female were found among all the different protein levels (P$lt;0.05). 2. Significantly less feed (P $lt;0.05) has been consumed by the chicks fed low level of dietary. protein in both male and female. Male consumed slightly more feed than female did, and significance was found at only 19-17% dietary protein level (P$lt;0.05). 3. No statistical difference (P$lt;0.05) was found among protein levels or between both sexes except female at 23-21% dietary protein level. But feed efficiency was improved Tightly as the level of dietary protein increased. 4. Utilizability of dry matter, crude protein, ether extract and NFE in the rations have been slightly improved with increment of dietary protein level. Increasing the dietary protein level resulted in improved nitrogen retention. 5. Feed cost per ㎏ body weight gain of chicks was the cheapest when the dietary level of protein was the lowest, and that of male was cheaper than that of female.
땅콩짚의 NaOH 처리에 의한 사료가치 개선에 관한 연구
명규호,김용식,선상수 ( K . H . Myung,Y . S . Kim,S . S . Sun ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2
To evaluate the effect of NaOH treatment on peanut straw of the different parts, chemical composition, in vitro dry matter digestibility (IVDMD, %) and in vitro organic matter digestibility (IVOMD, %) were tested. The parts of peanut straw were devided into four parts of blade, stem, hull and whole straw. The results obtained are summarized as follows; 1. The blade contained more crude protein, crude fat, nitrogen free extract and crude ash, but reverse phenomina were observed in the contents of crude fiber and cell wall constituents than the others. 2. In vitro dry matter digestibilities (IVDMD, %) of the four parts of NaOH-treated peanut straw were highly significantly different (p$lt;0.01) in different levels of NaOH treatment and of in vitro times. 3. In vitro organic matter digestibilities (IVOMD, %) of the four parts of peanut straw were showed the same tendency with the IVDMD`s (%).
명규호 한국영양사료학회 1981 韓國營養飼料學會誌 Vol.5 No.1
本 試驗은 國産 TCP, 外産 TCP, 外産 DCP 및 골분 등 國外産 인산칼슘제의 부로일러에 對한 成長率 및 칼슘, 인의 利用性을 究明하기 위하여 360首의 Hubbar系 초생추를 供試하여 5週間 실시하였다. 실험한 結果를 要約하면 다음과 같다. 1. 本 試驗에 使用된 骨粉 및 인산칼슘제의 칼슘과 인의 함량은 기산제 3인산 칼슘이 32% 및 18%였고 국산 제3인산 칼슘은 29% 및 15%에 이르는 것으로 나타났다. 弗素中毒症은 供試한 인산칼슘제를 사료의 2%까지 급여해도 나타나지 않았다. 2. 增體量에 있어서 대조구 I과 그외의 시험구들 간에 유의차가 있었으며 飼料效率에 있어서는 국산 제 3인산 칼슘구 외산제 2인산 칼슘구 및 골분구 간에 차이가 없었다. 3. 飼料攝取量에 있어서 대조구 I과 그외의 區들 간에 유의적 차이 (P< 0.05)를 보이지만, 그외의 처리 구간에는 통계적 차이는 없었으나, 인산칼슘제를 첨가 하므로서 증가하는 경향을 나타냈다. 4. 시험사료의 영양소 이용율은 모든 區間 차이가 없었으나 인산칼슘區들 및 골분구에서 다소 높은 경향이 나타났다. 5. 脛骨의 조회분 함량은 대조구I이 그외의 區들 보다 적었다(P<0.05). 脛骨 칼슘 함량에서는 全 區間에 차이는 없었으나 인 함량에서는 대조구I과 외산인산칼슘 區間에 유의차 (P<0.05)가 있었다. 6. 인의 공급 수준이 낮고 칼슘 : 인의 비율이 넓을수록 폐사율이 높았다. 以上의 結果에서 인산칼슘제 種類間 칼슘과 燐의 이용성은 거의 차이가 없었으며 부로일러에 魚粉의 低水準 급여시 인산칼슘제를 첨가하므로서 增體量, 飼料效率의 증가를 가져왔다.