프로스타글란딘F2α가 배양된 섬모체근세포의 산화질소 생성에 미치는 영향

김재우,김윤영,김근해,Jae Woo Kim,M,D,Yoon Young Kim,M,D,Keun Hae Kim,M,D 대한안과학회 2005 대한안과학회지 Vol.46 No.1

Purpose: To investigate whether prostaglandin F2α (PGF2α) affects on the production of nitric oxide (NO) in cultured ciliary muscle (CM) cells. Methods: Following primary culture of CM cells from porcine eyes, the cells were exposed to PGF2α (PhXA85, latanoprost free acid) with and without pretreatments of NO synthase inhibitor (L-NAME, Nω- Nitro-L-arginine methyl ester) and cyclooxygenase inhibitors (indomethacin and dexamethasone) for 3 days. The cellular survivals were also evaluated in serum-deprived and hypoxic conditions induced by sodium cyanide. The cellular survival and nitrite production were assessed by MTT and Griess assay, respectively. Results: PGF2α enhanced the production of NO significantly in cultured CM cells in a dose-dependent manner, while various inhibitors abolished this effect. PGF2α was not cytoprotective in serum-deprived and hypoxic conditions. Conclusions: The current results suggest that PGF2α potentiates NO production but is not cytoprotective in CM cells. This PGF2α-induced NO production in CM cells may be involved in the regulation of uveoscleral outflow.

공동주택 생산성 데이터 수집/분석을 위한 WBS 개발

김재우,김예상,김영석,김상범,Kim, Jae-Woo,Kim, Yea-Sang,Kim, Young-Suk,Kim, Sang-Bum 한국건설관리학회 2008 건설관리 : 한국건설관리학회 학회지 Vol.9 No.5

생산성은 모든 생산조직의 견실성 및 생산 활동의 효율성 평가를 위한 매우 중요한 경영 지표 중 하나로써 생산성의 기능은 건설 산업에 있어서도 예외일 수 없다. 그러나 국내의 경우 생산성의 중요성과 그 기능에도 불구하고 건설 생산성 관리와 관련된 사항은 대부분 현장관리자의 경험과 직관에 의존하고 있으며 생산성 데이터를 데이터베이스화하여 공사계획 및 관리에 적극활용하지 못하고 있다. 이러한 문제는 생산성 데이터와 관련된 정보의 수집 및 분석 방법이 체계적이지 못한 결과로, 생산성 데이터의 효과적인 활용을 위해 우선적으로 WBS개선이 이루어져야 한다. 따라서 본 연구에서는 연구문헌 분석, 해외사례 분석으로 연구에 적용함에 따른 적합여부 및 개선사항을 도출하고, 현장 인터뷰를 통해 현장여건을 반영한 후, 설정된 기본방향을 바탕으로 생산성 데이터 수집 및 분석을 위한 WBS를 구축하였다. 마지막으로 생산성 데이터 수집 및 분석을 위한 체계로의 검증을 실시하였다. 향후 구축된 WBS를 통해 수집된 생산성 데이터는 OLAP을 이용한 생산성 분석 및 Data Mining을 이용한 생산성 예측에도 활용될 것으로 기대된다. Productivity is one of key management indexes for evaluating soundness of a manufacturing organization and its efficiency. In many aspects of productivity management in the construction industry, however, intuition of an experienced field manager still plays a greater role while productivity data is not utilized efficiently for the construction management purposes, because the collection and analysis of the productivity-related information are not systematic. Lack of systematic method in collecting and analyzing the productivity data results in such problems. The existing WBS should therefore be improved to solve them. The authors developed a new WBS for productivity data collection and analysis by following the research direction that was determined by literature reviews, overseas cases, and interviews with field engineers. The new breakdown structure was then evaluated for its feasibility as a productivity analysis framework. It is expected that the productivity data collected by the WBS will be used for OLAP and mining for future productivity forecast.

과산화수소에 의한 산화스트레스가 섬유주세포의 노화에 미치는 영향

김재우,김신후,이재형,Jae Woo Kim,Sin Hoo Kim,Jae Hyung Lee 대한안과학회 2008 대한안과학회지 Vol.49 No.10

Purpose: To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence. Methods: Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-β-gal assays, respectively. Results: Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence. Conclusions: Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma. J Korean Ophthalmol Soc 2008;49(10):1665-1670

은행잎추출물이 배양된 테논낭 섬유아세포의 증식에 미치는 영향

김재우,김성재,강현구,Jae Woo Kim,M,D,Seong Jae Kim,M,D,Hyun Ku Kang,M,D 대한안과학회 2006 대한안과학회지 Vol.47 No.4

Purpose: To investigate the effect of Ginkgo biloba extract (GBE) on the proliferation of cultured human Tenon capsule fibroblasts (HTCF). Methods: Free radical scavenging activity of GBE was assessed with a DPPH assay. Primarily cultured HTCF were exposed to 10 and 100 ?g/ml of GBE, and the effect of this extract on HTCF survival was assessed. Following 48 hr exposure to the media with or without serum, cellular survival and nitrite production were assessed by MTT and Griess assays. To evaluate whether GBE had a cytoproptective effect, HTCF were cultured in a combination of GBE and either sodium cyanide or hydrogen peroxide. Results: GBE showed free radical scavenging activity. GBE increased the cellular survival of HTCF significantly in a dose-dependent manner and provided a cytoprotective effect when cells were exposed to sodium cyanide or were deprived of serum, but not when hydrogen peroxide was added to the medium. GBE decreased nitric oxide production but not to a statistically degree. Conclusions: GBE promotes proliferation of HTCF and has a cytoprotective effect in serum-deprived or hypoxic conditions. This suggests that GBE may be involved in the regulation of conjunctival wound healing by increasing the survival of HTCF.

선천 백내장 수술 후 발생한 망막박리

김재우,장우혁.Jae-Woo Kim. M.D.. Woo-Hyok Chang. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.11

Purpose: To analyze the preoperative clinical characteristics, surgical methods, outcomes and visual prognosis of retinal detachment following congenital cataract surgery. Methods: We retrospectively analyzed the medical records of 16 patients (16 eyes) who had undergone an operation for retinal detachment following congenital cataract surgery. Anatomical success was considered to have occurred if the retina was well attached in fundus exam at postoperative 6 months. Results: There were more male (81.3%) patients than female patients. The average age of cataract surgery was 19.3 years, and the mean interval between cataract surgery and retinal detachment was 7.7 years. The most common type of the retinal break was a round hole and the most common location of retinal break was the inferotemporal quadrant. Total retinal detachment was 56.2% and the macula was detached in 87.5% of patients. The anatomical success rate of the primary operation was 43.8% and the final success rate was 87.5%. Postoperative visual acuity increased in 43.8% of patients. Conclusions: Retinal detachment following congenital cataract surgery had a poor success rate compared to that of other retinal detachments due to difficulties in finding the retinal break (37.5%) and frequent total retinal detachment. Early diagnosis, prompt surgery, vitrectomy and intraocular tamponade for primary operation would increase the success rate and improve postoperative visual prognosis.

Target DNA 염기서열 내에 존재하는 비상동성 간격이 상동성재조합을 이용한 클로닝 빈도에 미치는 영향

김재우,도은주,윤세련,정윤희,윤영호,임선희,선우양일,박인호,Kim Jae-Woo,Do Eun-Ju,Yoon Se-Lyun,Jeong Yun-Hee,Yoon Young-Ho,Leem Sun-Hee,Sunwoo Yangil,Park In-Ho 한국미생물학회 2005 미생물학회지 Vol.41 No.4

Transformation-associated recombination (TAR) 클로닝 법은 복잡한 게놈으로부터 염색체 내의 특정부위나 유전자를 선택적으로 분리할 수 있다. 이 방법은 목적 유전자에 근접한 작은 게놈DNA 염기서열 정보를 필요로 한다. 이 기술은 효모의 spheroplast transformation을 시키는 동안 목적으로 하는 유전자의 5' 또는 3' 서열을 포함하고 있는 TAR vector와 게놈DNA사이에서 일어나는 상동성재조합에 의해 이루어진다. 본 연구에서는 plasmid 모델시스템을 이용하여 target hooks 내에 존재하는 비상동성 염기서 열이 상동성재조합에 미치는 영향을 조사하였다. plasmid에 존재하는HIS3유전자와 변형시킨 his3-TRP1-his3 단편 사이의 상동성재조합의 효율은 $Ura^+$ 형질전환체의 형질분석에 의해 이루어졌다. $Ura^+$ 형질전환체의 수는 7종류의 서로 달리 변형된 his3-TRP1-his3 단편들을 사용하였을 매 거의 동일하게 나타났다. 그러나 $Trp^+His^+$ positive recombinants의 빈도는 변형된 his3-TRP1-his3 단편 내에 비상동성 영역에 부정확한 간격을 지닐 때 현저한 감소를 나타내었다. 이러한 결과로서, 부정확한 간격이 target hook과 substrate DNA 사이에 일어나는 상동성재조합을 방해하는 것으로 사료된다. 그러므로 이종간의 상동유전자를 클로닝 할 때에는 target hook내의 비상동성 염기서열이 존재한다면 이것이 정확한 간격을 지니는지 여부를 중요란 요인으로 고려해야 한다. Transformation-Associated Recombination (TAR) cloning technique allows selective isolation of chromosomal regions and genes from complex genomes. The procedure requires knowledge of relatively small genomic sequences that reside adjacent to the chromosomal region of interest. This technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences. In this study, we examined the effect of non-homologous spacing sequence in target hooks on homologous recombination using a plasmid model system. The efficiency of homologous recombination between the modified his3-TRP1-his3 fragments and HlS3 gene on plasmid were analyzed by the characterization of $Ura^+$ transformants. The numbers of $Ura^+$ transformant showed same level when seven different modified his3-TRP1-his3 fragments were used. But the percentage of positive recombinants. $Trp^+His^-$, dramatically decreased when used the modified his3-TRP1-his3 fragments contained incorrect spacing of nonhomologous region. As a result, we suggest that incorrect spacing inhibits the homologous recombination between target hook and substrate DNA. Therefore, we should consider the correct spacing in target hook when the target hook are used for cloning of orthologue gene.

유리체절제술과 백내장 동시수술에서 네지지부인공수정체의 안정성

김재우,양지욱,지동현,Jae Woo Kim,Ji Wook Yang,Dong Hyun Jee 대한안과학회 2010 대한안과학회지 Vol.51 No.6

Purpose: To evaluate the stability of intraocular lenses (IOLs) according to the haptic design in combined surgery (phacoemulsification, intraocular lens insertion, and vitrectomy) by comparing early IOP elevation and the accuracy of IOL power. Methods: A retrospective study was conducted for 85 eyes of 85 patients who had undergone combined surgery. IOP elevation one week postoperatively and the difference between target refraction and postoperative refraction after two months were evaluated in all patients. All eyes were allocated to either a 2-haptic IOL group or a 4-haptic IOL group, and then each group was divided into three subgroups: gas-filled, silicone oil-filled, or balanced salt solution-filled. Results: In the balanced salt solution-filled group, the early IOP increase was significantly less in the 4-haptic IOL group than it was in the 2-haptic IOL group (3.24 mmHg vs. 6.15 mm Hg, p=0.025). The myopic shifts of the 2-haptic IOL group were significantly greater than those of the 4-haptic IOL group for all of the subgroups (gas subgroup -0.71D vs. -0.31D, p=0.045; balanced salt solution subgroup -0.66D vs. -0.16D, p=0.018). Conclusions: In combined surgery, 4-haptic IOL implantation showed significantly less early IOP elevation and less myopic shift than those of 2-haptic IOL implantation. J Korean Ophthalmol Soc 2010;51(6):829-834

Cyclosporin A가 in vitro에서 조골세포에 미치는 영향

김재우,이현정,강정화,옥승호,최봉규,유윤정,조규성,최성호,Kim, Jae-Woo,Lee, Hyun-Jung,Kang, Jung-Hwa,Ohk, Seung-Ho,Choi, Bong-Kyu,Yoo, Yun-Jung,Cho, Kyoo-Sung,Choi, Seong-Ho 대한치주과학회 2000 Journal of Periodontal & Implant Science Vol.30 No.4

Cyclosporin A(CsA) is an immunosuppressive agent widely used for preventing graft rejecting response in organ transplantation. The basic properties of CsA to osteoblast has not been well known yet. A better understanding of the mechanisms of CsA function on bone could provide valuable information regarding basic properties of bone remodeling, pharmacotherapeutic intervention in metabolic bone disease, and the consequences of immunosuppression in bone physiology. The purpose of this study was to investigate the effect of CsA on osteoblast by evaluating parameters of proliferation, collagen synthetic activity, alkaline phosphatase activity, and ALP mRNA expression in mouse calvarial cell. 1. CsA ($3{\mu}g/m{\ell}$) treated mouse calvarial cell showed statistically significant increase in cell proliferation.(P<0.05) 2. CsA($1,\; 3{\mu}g/m{\ell}$) treated MC3T3 cell line showed statistically significant increase in cell proliferation. 3. The amount of collagen of CsA($3{\mu}g/m{\ell}$) treated mouse calvarial cell was decreased statistically significantly. 4. Alkaline phosphatase activity was increased statistically significantly in CsA treated group($1{\mu}g/m{\ell}$). 5. mRNA expression of ALP was increased in CsA treated group These results suggest that CsA could affect bone remodeling by modulating proliferation & differentiation of osteoblast.

구강편평세포암종 세포주 SCC15에서 Mycolactone에 의한 apoptosis 효과

김재우,송재철,이희경,이태윤,Kim, Jae-Woo,Song, Jae-Chul,Lee, Hee-Kyung,Lee, Tae-Yoon 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.6

The effect of mycolactone, a recently reported apoptosis-inducing factor, was investigated in SCC15 oral squamous cell carcinoma(OSCC) cell line. Mycolactone rapidly induced cell death in OSCC cells in 2days, which was similar to that found in apoptotic cell such as detaching from culture plate and rounding-up of cells. Apoptotic cells were increased 4hrs after mycolactone treatment and more than half of cells showed apoptosis after 72hrs. Caspase 3 activation a biochemical evidence of apoptosis, was determined by Western blotting. Caspase 3 activation was started at 2hrs that lasted until 8hrs after mycolactone treatment. The expression of bcl-2 family genes was determined to explain the mechanism of apoptosis found in OSCC cells. The expressions of bad, bak, and bax (pro-apoptotic genes) and bcl-w and bcl-2 genes (anti-apoptotic genes) were not changed by mycolactone treatment. The expression of bcl-xi was decreased 8 hrs after mycolactone treatment. Mcl-1 expression was initially increased at 2 hrs which was decreased 8 hrs after mycolactone treatment. The down-regulation of these two anti-apoptotic genes might explain the mycolactone-induced apoptosis in OSCC cells. In this study, mycholactone was revealed to induce cell death in OSCC cells apoptosis and the apoptosis mechanism of OSCC cells was shown to be down-regulation of anti-apoptotic genes, bcl-xi and mcl-1. These results suggested the applicability of mycolactone for the development of an anti-cancer drug candidate by inducing apoptosis of OSCC cancer cell.

일산화질소가 섬유주세포에 의한 교원질 겔의 수축에 미치는 영향

김재우,장우석,이수윤,Jae Woo Kim,M,D,Woo Seok Chang,M,D,Su Yoon Lee,M,D 대한안과학회 2007 대한안과학회지 Vol.48 No.11

Purpose: To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs). Methods: After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively. Results: Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05). Conclusions: NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.