조하문의「내 아픔 아시는 당신께」

김애리 대중음악콘텐츠학회 2020 대중음악콘텐츠비평지 Vol.1 No.1

Mutations of the Epidermal Growth Factor Receptor Gene in Triple-Negative Breast Cancer

김애리,장민혜,이수정,배영경 한국유방암학회 2017 Journal of breast cancer Vol.20 No.2

Purpose: Epidermal growth factor receptor (EGFR) is considered a potential therapeutic target for anti-EGFR therapy in triple-negative breast cancer (TNBC). However, the frequency of EGFR gene mutation in TNBC is low and varies with ethnicity. This study aimed to investigate the incidence of EGFR gene mutation in TNBC. Methods: EGFR protein expression was evaluated by immunohistochemistry in tissue microarrays of 493 TNBC cases using four different primary antibodies, which included mutationspecific antibodies. For cases with an immunoreactivity level ≥1+, we performed pyrosequencing analysis for EGFR gene mutation. A case was considered mutation-positive when its mutation frequency minus its limit of detection (LOD) was >10%. Cases with mutation frequency higher than LOD were assessed for EGFR gene mutation status using the Cobas assay and by peptide nucleic acid-mediated polymerase chain reaction (PNA-clamping). Results: Among 493 TNBCs, 148 (30.0%) exhibited staining ≥1+ for EGFR, including 78 with 1+, 49 with 2+, and 21 with 3+. Positive EGFR expression (≥2+) was significantly associated with lymphovascular invasion (p=0.010), but not with overall survival (p=0.444) or disease-free survival (p=0.388). None of the 493 TNBCs harbored an EGFR gene mutation. Among 148 cases with an EGFR staining result ≥1+, five (3.4%) showed mutation frequencies (4.4%–10.9%) higher than LOD (2.6%–4.3%) in exons 19 (L747_P753>Q) or 21 (L858R and L861Q) as determined by pyrosequencing. However, Cobas and PNA-clamping failed to detect the presence of EGFR gene mutation in these five cases. Conclusion: No activating mutation of EGFR gene of clinical significance was observed in 148 TNBC cases using three commercially available methods. Thus, EGFR gene mutation appears to be an extremely rare event in patients with TNBC.

Multiplication of Chromosome 17 Centromere Is Associated with Prognosis in Patients with Invasive Breast Cancers Exhibiting Normal HER2 and TOP2A Status

김애리,신형찬,배영경,김민경,강수환,이수정,이은희 한국유방암학회 2012 Journal of breast cancer Vol.15 No.1

Purpose: This study aimed to investigate the clinical significance of chromosome 17 centromere (CEP17) multiplication (increased copy number of CEP17) related to human epidermal growth factor receptor 2 (HER2) and topoisomerase II alpha (TOP2A) status in patients with invasive breast cancer. Methods: We constructed tissue microarrays using 594 invasive breast cancer samples and performed single-color silver-enhanced in situ hybridization (SISH) assay for HER2, TOP2A, and CEP17 to assess for copy number aberrations. The association of CEP17 multiplication with patient survival was analyzed according to HER2 and TOP2A status. Results: Among 567 informative cases, HER2 amplification was noted in 22.8%, TOP2A amplification in 8.3% and TOP2A deletion in 11.1%. CEP17 multiplication was identified in 33.2% and was significantly associated with worse overall survival (OS) (p=0.02) and disease-free survival (DFS) (p=0.02). CEP17 multiplication correlated with patient survival in patients with normal TOP2A or non-amplified HER2 status, but the prognostic significance was lost in those with altered TOP2A or amplified HER2. On multivariate analyses, CEP17 multiplication was an independent prognostic factor for poorer OS (p=0.02)and DFS (p=0.01) in patients with normal TOP2A and non-amplified HER2. Conclusion: CEP17 multiplication was identified as a promising prognostic marker in patients with invasive breast cancer exhibiting either non-amplified HER2 or normal TOP2A status.

Phosphorylated Akt and Phosphorylated mTOR Expression in Breast Invasive Carcinomas: Analysis of 530 Cases

김애리,안정석,정회선,이영석,우상욱,서재홍 한국유방암학회 2010 Journal of breast cancer Vol.13 No.4

Purpose: The phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway has a central role in regulation of cell proliferation, differentiation, motility and survival. This pathway has recently generated great interest because its elements are, potentially, novel targets for the treatment of various malignancies, including breast cancer. Methods: Using tissue microarray sections of breast carcinoma, we performed immunohistochemical studies using antibodies against the phosphorylated forms of Akt (p-Akt) and mTOR (p-mTOR) in 530 invasive breast carcinomas and 30 ductal carcinomas in situ (DCIS). We investigated possible associations between expression of these proteins and clinicopathologic characteristics and disease outcomes. Results: In 530 invasive carcinomas, weak and strong expression of p-Akt was observed in 180 (34.0%) and 288 (54.3%) cases, respectively. The expression of p-Akt was associated with expression of estrogen receptors (ER) (p=0.045), progesterone receptors (PR) (p=0.003), lymph node metastasis (p<0.001) and cancer stage (p=0.027). Weak and strong expression of p-mTOR was found in 136 (25.7%) and 207 (39.1%) cases, respectively. The mTOR pathway was more frequently activated in DCIS than in invasive breast carcinoma (p=0.001). p-mTOR expression was associated with expression of ER (p=0.040), PR (p=0.009), tumor size (p<0.001), and stage (p=0.002). In a univariate analysis, strong expression of p-Akt was associated with longer disease-free survival (DFS). In a multivariate analysis, neither p-Akt nor p-mTOR was associated with DFS. Conclusion: The PI3K/Akt/mTOR pathway is active in DCIS as well as in invasive carcinoma of the breast. Our study also suggests that the PI3K/Akt/mTOR pathway is influenced by ER rather than erbB-2, and that this pathway may contribute more to cancer pathogenesis in ER-positive tumors.

NLP기법을 활용한 집단미술치료프로그램이 저소득층 중학생의 자기주도학습 준비도에 미치는 효과

김애리,유경미 한국예술심리치료학회 2019 예술심리치료연구 Vol.15 No.4

본 연구는 저소득층 중학생의 자기주도학습 준비도(self-directed learning readiness)에 대한 NLP기법을 활용한 집단미술치료프로그램을 통해 그 효과성을 검증하고자 하였다. 연 구대상은 00도에 소재하고 있는 중학교 교육복지우선 지원 사업 대상자 중, 참여를 희망하 여 참가동의서와 신청서를 제출한 학생 24명(실험집단 8명, 비교집단 8명, 통제집단 8명)으 로 선정하였다. 실험설계는 통제-비교집단 사전사후설계를 적용하였으며 실험집단은 NLP 기법을 활용한 집단미술치료프로그램을, 비교집단은 집단미술치료프로그램을 실시하였다. 자료처리는 SPSS 22.0 프로그램을 활용하여 자기주도학습 준비도 검사의 평균과 표준편차 를 구하기 위해 기술통계를 실시하였으며, 세 집단 간에 실험집단이 효과가 있는지 검증하 기 위해 공분산분석(ANCOVA)을 실시하였다. 본 연구의 결과로 첫째, NLP기법을 활용한 집단미술치료프로그램은 저소득층 중학생의 자기주도학습 준비도 사후검사 전체점수에서 실험집단이 통제집단보다 통계적으로 유의미한 차이가 있었으며, 자기주도학습 구성요인의 자아개념과 창의성 척도점수가 유의미하게 향상되었음을 알 수 있었다. 이는 미술 매체 활 동을 통한 집단미술치료의 치료적 기법과 NLP 무한성취 가능성의 치료적 기법이 통합되어 자기주도학습의 준비를 위한 긍정적 생각의 전환 효과가 있었으며 학생들이 학습의 주체자 로서 역할에 첫걸음인 자기주도학습 준비도 능력 향상에 도움이 되었다. The purpose of this study is to prove the effectiveness of group art therapy with NLP technic on self-directed learning readiness of lower-income group students in a middle school. The subjects of this study w lower-income group students in a middle school and the program in this study was adapted for the. The were 24 students(8 of experimental group, 8 of comparative group, 8 of controled group) of 00 middle school in 00 Province, and they agreed to participate in this study. ontrol group pretestposttest design was adopted in this study. The experimental group students participated in the program of group art therapy with NLP, and the comparative group students in the program of group art therapy without NLP. In order to find out average value and standard deviation of self-directed learning readiness, descriptive statistics were implemented with SPSS 22.0 program. to prove the difference between the three groups results, ANCOVAwas applied. he result of this study is the self-directed learning readiness of experimental group students(lower income group students who participated in the group art therapy program with NLP) was generally higher than those of comparative group students(lower income group students who participated in the group art therapy program without NLP) and controlled group students, in the posttest data. This study tried to improve self-directed learning readiness by integrating group art therapy and NLP technic in the clinical field. This is an early study in its area.

광주광역시 공개 소프트웨어 시범적용사업 구축사례

김애리 한국지역정보개발원 2007 지역정보화 Vol.44 No.-

히스톤 메틸화와 유전자 전사

김애리,Kim, Ae-Ri 한국생명과학회 2007 생명과학회지 Vol.17 No.4

Amino acids of histone tail are covalently modified in eukaryotic cells. Lysine residues in histone H3 and H4 are methylated at three levels; mono-, di- or trimethylation. Methylation in histones is related with transcription of the genes in distinct pattern depending on lysine residues and methylated levels. Relation between transcription and methylation has been relatively well understood at three lysines H3K4, H3K9 and H3K36. H3K4 is methylated in active or potentially active chromatin and its methylation associates with active transcription. H3K9 is generally methylated in heterochromatin or repressed gene, but trimethylation of this lysine occur in actively transcribed genes also. Methylation at H3K36 generally correlates with active chromatin/transcription, but the correlation of its dimethylation with transcription is controversial. All together methylation patterns of individual lysine residues in histone relate with activation or repression of transcription and may provide distinctive roles in transcriptional regulation of the eukaryotic genes.

자기주도적 집단미술치료프로그램이 저소득층 중학생의 학습전략에 미치는 효과

김애리,유경미 한국아동발달지원연구소 2016 임상미술심리연구 Vol.6 No.2

본 연구는 자기주도적 집단미술치료프로그램이 저소득층 중학생의 학습전략에 미치는 효과성을 검증하고자 하였다. 연구대상은 충청북도 00지역에 소재하고 있는 B중학교 2, 3 학년 교육복지우선 지원 사업 대상자 중, 참여를 희망하는 참가동의서와 신청서를 제출한 학생 18명(실험집단 9명, 통제집단 9명)으로 선정하였다. 실험설계는 통제집단 사전사후설 계를 적용하였으며 실험집단에게 자기주도적 집단미술치료프로그램을 주 1회 매 회기 90 분씩 20회기 동안 실시하였다. 연구도구는 학습전략검사를 사전․사후 비교하여 검증하였다. 자료처리는 SPSS 22.0프로그램을 활용하여 공변량분석을 하였다. 본 연구의 결과는 다음과 같다. 첫째, 자기주도적 집단미술치료프로그램은 저소득층 중학생의 성격특성에서 유 의미한 차이가 있었다. 둘째, 자기주도적 집단미술치료프로그램은 저소득층 중학생의 동기 특성에서 유의미한 향상을 보이지 않았다. 셋째, 자기주도적 집단미술치료프로그램은 저소득층 중학생의 정서특성에서 유의미한 향상을 나타냈다. 넷째, 자기주도적 집단미술치료프 로그램은 저소득층 중학생의 행동특성에서 유의미한 보이지 않았다. 다섯째, 자기주도적 집단미술치료프로그램은 저소득층 중학생의 학습전략지수에 유의미한 향상을 보였다. 따라서 본 연구에서 적용한 자기주도적 집단미술치료프로그램은 저소득층 중학생의 학습전략 과 하위요인의 일부에서 긍정적인 영향을 미칠 수 있음을 확인하였다. The study of self-directed learning group art therapy program was aimed to verify the effectiveness of learning strategies for the middle school students in low income bracket. Eighteen students (experimental group 9 and control group 9) were selected who were second or third grade of B middle school in 00 area in Chungcheong buk province. Pretest-posttest design was applied. Self-directed learning group art therapy program was given once a week for ninety minutes each and twenty times. Study tool was verified comparing learning strategies test pre-to post. SPSS 22.0 program was used for the data processing, which was analyzed by ANCOVA. The results of the study are following. First, self-directed learning group art therapy program showed up significant improvement personality traits that the middle school students in low income bracket have. Second, it did not show significant improvement on motivational characteristic of the middle school students of low income bracket. Third, it showed significant improvement on emotional characteristic of the middle school students of low income bracket. Fourth, it did not show significant improvement on behavioral characteristic of the middle school students of low income bracket. Fifth, it showed significant improvement on learning strategies of the middle school students of low income bracket. Therefore, the study applied self-directed learning group art therapy program showed up positive effects to the some characteristic factors of the middle school students' learning strategies of low income bracket.

대장의 양성 및 악성종양에서 Bcl-2 종양단백의 발현과 p53 종양단백 및 Apoptosis와의 상관성에 관한 연구

김애리,조성진,원남희,채양석 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.5

구조화된 상지운동이 혈액투석 환자의 동정맥루 협착에 미치는 효과

김애리 기초간호학회 2012 Journal of korean biological nursing science Vol.14 No.4

최근 과학과 의료의 발달 및 수명 연장에 따른 노인인구의 증가로 인해 신부전에 이환될 가능성이 높아졌으며, 말기 신부전증의 주요 원인인 당뇨병과 고혈압 환자가 증가하고 있어, 신 대체 요법을 필요로 하는 말기 신부전증 환자가 급격히 증가하고 있다(Kim, 2008). 대한 신장 학회 등록위원회(2010)의 조사에 의하면 국내의 신 대체요법을 받고 있는 말기 신부전증 환자 수는 해마다 증가하여 처음 조사된 1986년에 2,534명이던 환자수가 2009년에는 56,396명으로 22배 이상으로 되었으며 계속 증가할 전망이다. 이중에서 혈액투석을 받고 있는 환자 수는 37,391명으로 66.3%를 차지하고 있다. 혈액투석의 효율을 높이기 위해 무엇보다 적절한 혈관통로의 조성 및 기능유지는 매우 중요한데(Hwang, 2006; Kim, 2008; Park, 2002) 동정맥루 이용 혈관은 제한되어 있기 때문에(Lee, 2004; Moon et al., 2006) 사용 혈로확보를 위한 혈관의 관리가 혈액 투석환자 간호 중 중요한 문제로 대두되고 있다.