넙치, Paralichthys olivaceus의 미세플라스틱 급성 노출에 따른 혈액성상 및 혈장성분의 변화

김소희,김가현,김지수,김준환,전유현,조재황,김석렬,김대희,Kim, So-Hee,Kim, Ga-Hyun,Kim, Ji-Su,Kim, Jun-Hwan,Jeon, Yu-Hyeon,Cho, Jae-Hwang,Kim, Seok-Ryel,Kim, Dae-Hee 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.3

Juvenile olive flounder, Paralichthys olivaceus (mean weight 66.7±7.1 g; mean length 19.2±0.9 cm) in a bio-floc environment were exposed to microplastic (PE: polyethylene, size 40-48 ㎛) at 0, 4, 20, 100, 500 and 2,500 mg L^-1 for 96 hours. No P. olivaceus deaths were observed following microplastic exposure. In the plasma components, calcium was significantly decreased whereas there was no significant change with magnesium following microplastic exposure. Glucose was significantly decreased with over 100mg L^-1 at 48 hours and 20mg L^-1 at 96 hours. Cholesterol was significantly decreased with over 20mg L^-1 after 48 hours, whereas there was no significant change in the total protein content. In enzymatic plasma components, the AST(Aspartate aminotransferase) was significant decreased by microplastic exposure. The results of this study indicate that acute exposure to microplastic induces blood physiological changes in P. olivaceus. 본 실험에서 미세플라스틱의 노출은 넙치의 혈액학적 성상인 hemoglobin 및 hematocrit 수치의 유의적인 감소를 유발하였다. 또한 미세플라스틱의 노출은 넙치 혈장 무기성분인 calcium, 유기성분인 glucose 및 cholesterol, 효소성분인 AST의 유의적인 변화를 나타냈다. 본 연구에서 바이오플락 환경에서 미세플라스틱의 노출이 어류의 혈액생리에 영향을 주며 독성으로 작용하는 것을 확인할 수 있었다. 향후, 일반 해수와 바이오플락 환경으로 각각 양성한 넙치를 이용하여 사육환경의 변화에 의한 미세플라스틱 노출영향의 차이에 대해서도 추가적인 연구가 이루어져야 할 것이다. 본 연구는 다양한 어종에서 종별 미세플라스틱의 농도 연구에서 미세플라스틱 노출에 따른 종별 독성영향비교평가를 위한 기본 자료로 활용될 수 있을 것이다. 하지만, 바이오플락 환경에서 미세플라스틱의 응집은 미세플라스틱의 독성에 영향을 미치는 중요한 요소이기 때문에(Choi et al. 2020), 향후 연구에서 철저한 모니터링과 함께 다양한 미세플라스틱 독성에 미치는 요소에 대한 추가연구가 필요할 것이다.

우리나라 청소년의 수면시간 및 질과 체질량지수와의 관련성

김소희,김유숙,장영희,박종,류소연,Kim, So-Hui,Kim, Yu-Suk,Jang, Young-Hee,Park, Jong,Ryu, So-Yeon 한국학교보건학회 2012 韓國學校保健學會誌 Vol.25 No.1

Purpose: This study aims to discover the relation between the sleep duration and quality of adolescents and their body mass index. Methods: The study used data obtained through the 2009 online survey on adolescents health behaviors, targeting a total of 75,066 middle and high school students (39,612 of male students and 35,454 of female students). We first performed t-test, ANOVA and Scheffe's test and then the potential variables which was possibly related to the body mass index are determined from the tests. Using the potential variable, we performed the multiple regression analysis to finally identify the relation between the duration and quality of sleep. Results: As a result, the 39.8% of male students and the 46.5% of female students most frequently respond that the sleep duration was less than 6 hours, and 36.4% of male students and the 45.3% of female students respond that the quality of sleep was not good enough. The body mass index of both the male and the female students portionally decreases to their sleep duration, but the better quality of sleep makes their body mass index higher. Conclusion: In conclusion, the body mass index has some notable relationship with the sleep duration and quality for adolescents. It is advised to consider the sleep duration and quality in the business plans for adolescents in order for the prevention of their body mass index.

15년간(1991~2005년) 우리나라 소아에서 분리된 폐구균의 혈청형 분포의 변화

김소희,송은경,이준호,김남희,이진아,최은화,이환종,Kim, So-Hee,Song, Eun-Kyung,Lee, Jun-Ho,Kim, Nam-Hee,Lee, Jin-A,Choi, Eun-Hwa,Lee, Hoan-Jong 대한소아감염학회 2006 Pediatric Infection and Vaccine Vol.13 No.2

목 적 : 폐구균 단백 결합 백신이 개발되었지만 이에 포함되는 혈청형은 7가지로 한정되어 있어, 지역별 주요 폐구균 혈청형의 분포에 따라 백신의 유용성에 차이가 있을 수 있다. 본 연구에서는 우리나라 소아에서 분리된 폐구균의 혈청형의 분포를 분석하고 지난 15년간 시기에 따른 변화 양상을 분석하고자 하였다. 방 법 : 1991년 1월부터 2005년 5월까지 서울대학교 어린이병원 소아 환자의 각종 임상 검체에서 분리된 폐구균 465균주의 혈청형을 quellung reaction으로 결정하였고, penicillin 감수성은 oxacillin 디스크 확산법으로 검사하였다. 연구기간은 1991~1995년(I분기), 1995~2000년(II분기), 2001~2005년(III분기) 등 3개의 분기로 나누어 분석하였다. 결 과 : 465균주의 주된 혈청형은 19F(22%), 23F(18%), 19A(10%), 6B(8%), 14(7%), 6A(7%), 9V(5%) 등이었고, 전체 균주의 79%이었다. 60개월 미만 연령군의 침습성 감염 검체에서 분리된 101균주에서의 주된 혈청형은 혈청형 23F(21%), 14(17%), 19F(15%), 19A(11%), 6B(10%), 6A(7%), 9V(7%) 등으로 총 70%이었다. 전체 균주에서 I, II, III의 각 분기별 혈청형 분포는 백신 혈청형에 포함된 혈청형 19F, 14가 감소하는 추세이었고, 교차 반응 혈청형에 포함된 혈청형 6A, 19A가 증가하는 추세이었다. 침습성 감염 균주에서 폐니실린 내성률은 백신 혈청형에서 92.6%, 교차 반응 혈청형에서 75.0%로 높았으나, 비백신 혈청형에서는 34.4%로 낮았다. 결 론 : 최근 15년간 소아의 임상 검체에서 분리된 폐구균의 혈청형은 주로 19F, 23F, 19A, 6B, 14, 6A, 9V 등이 가장 흔하였고, 최근에 혈청형 19A, 6A가 증가하는 추세이고, 혈청형 19F와 14가 감소하였다. 향후 백신 사용과 관련하여 국내 폐구균 혈청형의 분포에 관한 지속적인 관찰이 필요하다고 생각된다. Purpose : The prevalence of serotypes can be changed with respect to age of the patients, regions and times of the surveillance. The aim of this study is to analyze changes in serotype distribution of pneumococcal isolates from Korean pediatric patients. Methods : Four hundred and sixty five strains of S. pneumoniae were isolated from various clinical specimens at the Seoul National University Children's Hospital during a 15 year-period, from 1991 to 2005, were subjected to serotype and penicillin susceptibility. The study period was divided into three 5 year-periods, 1991~1995(period I), 1996~2000(period II), and 2001~2005(period III). Results : Common serotypes were 19F, 23F, 19A, 6B, 14, 6A and 9V in decreasing orders of frequency, and these 7 serotypes accounted for 79% of total 465 strains. In the serotype distribution of invasive infection isolates from children <60 months of age, similar serotypes accounted for 70% of 101 strains. In total strains, the proportion of serotype 19A and 6A increased, while that of serotype 19F and 14 decreased. The majority(75~92%) of vaccine types and cross-reactive types from invasive infection isolates was penicillin-resistant, whereas 34.4% of non-vaccine types was penicillin-resistant. Conclusion : Distribution of serotypes of S. pneuomoniae isolated from Korean children has changed significantly over the last 15 years. The surveillance for pneumococcal serotypes should be continued to monitor changes in serotype distribution which are essential for the establishment of pneumococcal vaccine policy.

시판 보냉팩 개수에 따른 진공포장 냉동 넙치(Paralichthys olivaceus) 및 고등어(Scomber japonicus) 필렛의 상온유통 중 선도유지능 비교평가

김소희,이지운,전은비,김진,박신영,이정석,So Hee Kim,Ji Un Lee,Eun Bi Jeon,Jin Kim,Pantu Kumar Roy,Shin Young Park,Jung-Suck Lee 한국수산과학회 2023 한국수산과학회지 Vol.56 No.6

The surge in online seafood consumption has increased parcel delivery, leading to a need to implement effective preservation methods. As the cold chain system is not fully established in Korea, styrofoam boxes and cold packs are commonly used for low-temperature seafood distribution. The impact of cold packs on product preservation depends on the number utilized. Herein, the freshness of vacuum-packed frozen fish fillets (Paralichthys olivaceus and Scomber japonicus) stored at 25±0.5℃ for up to 84 h was measured. Chemical (pH and volatile base nitrogen), microbiological (viable cell count), and physical (odor intensity) properties were assessed using 2 or 4 cold packs in a styrofoam box. Four cold packs yielded lower values, indicating superior freshness, and extended fish freshness by approximately 12 h compared with two cold packs. Therefore, it is recommended to use a minimum of 4 cold packs (-350 g/cold pack) in a styrofoam box for distributing approximately 300 g of frozen fish fillets at room temperature during the summer, considering an average delivery period of 2 days in Korea.

생달나무 잎 추출물 유래 항염 및 항산화 활성 성분

김소희,김정은,이남호,Kim, So Hee,Kim, Jung Eun,Lee, Nam Ho 대한화학회 2021 대한화학회지 Vol.65 No.1

본 연구에서는 생달나무 잎 추출물 및 용매 분획물의 항염 및 항산화 효능을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. RAW 264.7 세포를 이용한 분획 시료의 NO 생성 억제 활성 실험 결과, EtOAc 분획물이 세포 독성 없는 농도에서 NO의 생성을 억제시키는 효능을 확인하였다. 또한 EtOAc 분획물이 iNOS 단백질의 발현 및 전염증성 사이토카인(TNF-α, IL-1β) 생성 저해 효과를 나타내었다. 항산화 활성 실험 결과, EtOAc 분획물이 DPPH 및 ABTS+ 라디칼 소거능이 우수함을 확인하였다. EtOAc 분획물의 활성 성분을 규명하기 위해 컬럼 크로마토그라피를 수행하여 5개의 화합물을 분리하였다; (4S,5R)-4-hydroxy-5-isopropyl-2-methylcyclohex-2-enone (1), methoxy-(3,5-dimethoxy-4-hydroxyphenyl)ethanediol (2), afzelin (3), nicotiflorine (4), narcissin (5). 화합물 1-5는 모두 생달나무에서 처음으로 분리되었다. 분리 화합물에 대한 항염 활성 실험 결과, 화합물 1, 3, 4 및 5는 세포 독성 없이 NO 생성 저해 효과를 나타내었다. 또한 화합물 1은 염증 매개인자인 TNF-α, IL-1β, IL-6의 생성 억제 활성을 보여주었다. 이상의 연구결과를 바탕으로 생달나무 잎은 항염 및 항산화 효과를 갖는 의약품 및 화장품 관련 천연 소재로의 개발이 가능할 것이라 사료된다. In this study, the extract of Cinnamomum yabunikkei leaves were investigated for the anti-inflammatory and anti-oxidative activities and their active constituents were identified. In the anti-inflammatory tests using LPS-stimulated RAW 264.7 cells, the ethyl acetate (EtOAc) fraction inhibited the production of nitric oxide (NO) without causing cell toxicity. In addition, the EtOAc fraction reduced expression of iNOS protein and production of pro-inflammatory cytokines (TNF-α, IL-1β). Upon the anti-oxidative studies by DPPH and ABTS+ radicals, potent radical scavenging activities were observed in the EtOAc fraction. Five phytochemicals were isolated from the extract of C. yabunikkei leaves; (4S,5R)-4-hydroxy-5-isopropyl-2-methylcyclohex-2-enone (1), methoxy-(3,5-dimethoxy-4-hydroxyphenyl)ethanediol (2), afzelin (3), nicotiflorine (4) and narcissin (5). As far as we know, compounds 1-5 were isolated for the first time from this plant. In the anti-inflammatory tests for the isolates, compound 1, 3, 4 and 5 were determined to decrease NO production without causing cell toxicity. Furthermore, compound 1 reduced expression of iNOS protein and exhibited potent inhibitory activities of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). Based on these results, it was suggested that the extract and isolated compounds from C. yabunikkei leaves could be potentially applicable as natural source for pharmaceutical and/or cosmetic ingredients.

베트남 결혼이주여성의 문화적응 스트레스와 자녀양육효능감의 관계에서 배우자 지지의 영향

김소희,노윤구,Kim, So Hee,Noh, Yoon Goo 한국여성건강간호학회 2018 여성건강간호학회지 Vol.24 No.2

Purpose: To identify the effects of spousal support on the relationship between acculturative stress and sense of parenting competence among married Vietnamese immigrant women. Methods: The sample for this study consisted of 175 married Vietnamese immigrant women in Gyeongsangnam-do. Data were analyzed using descriptive statistics, t-test, ANOVA, $Scheff{\acute{e}}$ test, Pearson correlations, and hierarchical multiple regression with SPSS/WIN 23.0. Results: (a) sense of parenting competence was negatively correlated with acculturative stress, whereas a positive correlation was observed with spousal support; (b) spousal support mediated the relationship between acculturative stress and sense of parenting competence. Conclusion: Results of the study suggest that spousal support decreases the negative effects of acculturative stress on sense of parenting competence. Therefore, we need to find ways to strengthen spousal support for married Vietnamese immigrant women.

간호대학생의 메타인지, 학습동기, 학업적 자기효능감이 자기주도학습능력에 미치는 영향

김소희(So Hee Kim), 이정민(Jung Min Lee), 김민경(Min Gyeong Kim), 김수아(Su Ah Kim), 김주은(Ju Eun Kim), 노윤서(Yun Seo Noh), 송지민(Ji Min Song), 이다원(Da Won Lee), 이소희(So Hee Lee) 다문화건강학회 2023 다문화건강학회지 Vol.13 No.1

Purpose: This study sought to identify the influence of metacognition, learning motivation, and academic self-efficacy on the self-directed learning ability of nursing students. Methods: A descriptive study design was used, and data were collected using self-reported questionnaires from 194 nursing students. Statistical analyses, including independent t-test, one-way ANOVA, Pearson's correlation, and multiple regression analysis, were performed using SPSS 26.0. Results: Metacognition, learning motivation, and academic self-efficacy significantly predicted nursing students' self-directed learning ability, accounting for 66.3% of the variance. Conclusion: Interventions to improve the self-directed learning ability of nursing students should focus on enhancing metacognition, learning motivation, and academic self-efficacy.

수은이 Lactobacillus bulgaricus의 젖산 생성과 생육 저해작용에 대한 양파 추출물의 효과

김소희(So Hee Kim),서명자(Myung Ja Suh) 한국생약학회 1987 생약학회지 Vol.18 No.1

Onion extracts were tested for effects on the growth and lactic acid production of Lactobacillus bulgaricus in the medium containing mercury. The media containing mercury were added with onion extracts and inoculated with the bacterium and then incubated at 39 for four days. All of the onion extracts examined increased the growth and lactic acid production of the bacterium in the medium containing mercury. At the addition of the edible portion extract of onion to the medium containing 5 ppm, 10 ppm and 20 ppm mercury, the higher the concentration of the onion extract was added, the greater the increasing effect on the growth and lactic acid production. The brown peel extract of onion increased the growth and lactic acid production of the bacterium in the medium containing mercury at all concentrations. The higher the concentration of the extract added, the more effective the increasing effect. The onion solutions of edible portion and brown peel extracted at 90 to 100 showed more desirable effects than those extracted at the room temperature. Among four kinds of the onion extracts, the brown peel solution extracted at 90 to 100 was the most effective in increase of the growth and lactic acid production in the medium containing mercury.

사회 복지 서비스 욕구에 관한 서비스제공자와 수혜노인의 인지비교연구

김소희(So Hee Kim),김미혜(Mee Hye Kim) 한국노년학회 1993 한국노년학 Vol.13 No.2

본 연구는 현재 지역사회 내에서 거주하고 있는 노인에게 제공되는 사회복지 서비스의 현황을 파악하고 노인에 대한 서비스욕구를 측정함으로써 노인의 욕구에 부합되는 서비스가 제공될 수 있도록 서비스 개발의 방향을 모색하기 위하여 현재 노인에게 이용가능한 서비스의 종류 및 그 실시횟수를 살펴보고 현존 서비스의 적절성 및 필요성을 조사하였다. 연구방법은 구조화 된 질문지를 이용하여 서베이조사(Survey Research)를 하였으며, 조사대상은 서비스 제공자인 38명의 사회복지사와 사회복지 서비스를 제공받고 있는 노인 43명이 표집되어 조사되었다. 노인에 대한 사회복지 서비스의 종류에 있어서 19가지의 서비스가 제공되고 있었으며, 50%이상의 실시율을 보이는 서비스는 재가노인 서비스, 무료진료, 개별상담, 단체급식 등을 포함한 8가지로서 당면문제의 해결을 위한 서비스가 많았다. 서비스제공자인 사회복지사와 수혜노인 모두 노인학교, 개인취미활동 지도, 경로잔치 등의 주로 발달욕구충족을 위한 서비스에 있어서 현재 적절하게 제공되는 상태로서 인지하고 있었으나, 현존 서비스의 필요정도에 있어서 사회복지사와 노인 모두 단체급식, 무료진료, 물리치료 등과 같은 당면문제의 해결을 위한 서비스의 필요정도가 높음을 나타냈다. 결국 현 서비스의 적절성에 대한 평가와 필요정도에 대한 평가가 서로 일치하지 않음을 보여줌으로써 노인에 대한 사회복지 서비스가 효과적으로 제공되고 있지 못함을 알 수 있다. 이상과 같은 연구결과들은 노인에 대한 사회복지 서비스의 개발 방향을 설정하는 데에 있어 하나의 근거자료로서 활용될 수 있을 것이다. The purpose of this research is to set the direction of social services development to meet the senior citizens' social service needs through understanding the current social services provided to the elderly in community and assessing their needs for services. Therefore, the variety of the services available to them, relevance of current service and needs for service are explored. For this research all 38 social workers on Social Welfare Institutes in Seoul and 43 elders out of those receiving social service are selected. Total of 19 different kinds of social services are being provided to the senior citizens. The services provided by above 50% of social workers are 8 services including in-home service, free health examination etc. In evaluating the relevance of the current services, social workers and clients have same opinion on most of provided services, and class for the elderly sponsoring leisure activity, party for the elderly are high scored by both. The social workers show relatively high level of needs of current services than the clients. However, the needs for several services are scored highly by both social worker and the elderly as follows ; providing free feeding, bathing, hair-cut, health examination, and physiotherapy service. These services can be classified as the solution services for urgent problems. The degree of relevancy of services is not corresponding to that of needs. Therefore, current provided services for the elderly are not effective. Above results can be used as the background information in setting the direction of development social services for the elderly.

변동성을 고려한 알칼리 수전해 시스템의 최적 운영 효과 분석

김소희(So-Hee Kim),손성용(Sung-Yong Son) 대한전기학회 2023 대한전기학회 학술대회 논문집 Vol.2023 No.7