타고난 지능의 필요성에 대한 청소년의 믿음과 학문 분야에 따른 성별 분포와의 관계

김서우,전지현,진경선 한국심리학회 산하 한국발달심리학회 2024 한국심리학회지 발달 Vol.37 No.2

여러 사회에서 여성이 이공계(STEM)를 기피하는 현상을 우려하고 있다. 그러나 학문 분야별 성 불균형을 좀 더 자세히 살펴보면 STEM여부와 관계없이 특정 분야에서 여성 박사가 남성 박사보다 적게 배출된다. 이러한 분야별 성 불균형에 대한 한 가지 설명인 영역특정적 능력에 대한 믿음 가설은 성공에 타고난 지능이 필수로 지각되는 분야일수록 여성이 적게 진출할 것을 예측한다. 본 연구는 영역특정적 능력에 대한 믿음의 발달적 궤적을 살펴보고자 한국 고등학생(N = 282)을 대상으로 30개 분야에서 성공하기 위해 타고난 지능이 얼마나 필요하다고 생각하는지 묻는 설문을 실시하였다. 이후 이러한 믿음과 해당 분야의 여성 박사 비율(교육통계서비스) 간의 관계를 알아보았다. 분석 결과, 학생들이 성공에 타고난 지능이 필수적이라고 생각할수록 실제 해당 분야의 여성 박사 비율이 낮았다. 또한, 학생들의 영역특정적 능력에 대한 믿음과 전공 선택 의향 간 관계에 성차가 나타났다. 남학생의 경우, 타고난 지능을 요구한다고 지각된 분야일수록 높은 전공 선택 의향을 보였지만, 여학생은 이러한 관계가 관찰되지 않았다. 이러한 결과는 영역특정적 능력에 대한 믿음 가설을 지지한다. Women‘s underrepresentation in STEM fields is a concern in many societies. However, closer inspection of this gender gap reveals a more complex picture, with women achieving parity with men at the Ph.D. level in certain STEM fields, while also being underrepresented in some non-STEM fields. The field-specific ability belief (FAB) hypothesis aims to explain this variability across academic disciplines and suggests that women are underrepresented in fields where raw intelligence is seen as required for success. This study explored the FABs of 282 Korean high school students. We found that the academic fields believed by adolescents to require brilliance are the fields with lower female representation. Moreover, there was a gender difference in how these beliefs were related to students’ interests in majors. Male students were more inclined to choose majors in fields with high FABs, whereas female students were not. These findings support the field-specific ability belief hypothesis.

크론병 환자에서 azathioprine으로 유발된 췌장염

김서우,정성애,권경주,류민선,노선희,태정현,최주영,김성은,정혜경,심기남,김태헌,유권,문일환 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-

결핵의 감별 진단을 위한 객담 검체에서 Real Time PCR의 임상적 유용성

김서우,장중현,심윤수,류연주,이진화 대한결핵 및 호흡기학회 2011 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.112 No.-

교복 설계를 위한 여자 청소년의 체형 변화 연구

김서우,남윤자,김경선 한국의류학회 2020 한국의류학회지 Vol.28 No.3,4

In this study, the 4th (1997) and 6th (2013) direct measurements of Size Korea compared the changing shape of women between the ages of 12 and 18. Comparing the items of height and length, the height and shoulder height were significantly reduced, the waist height was not significantly different, and the height of the upper hip was significantly increased, resulting in a smaller upper torso ratio and a higher lower torso rate. The width and thickness associated with human obesity, the circumference items and obesity levels often increased significantly with the change of the times, indicating that the overall body size was increased and that the chest area was changed to a cylindrical shape with changes in the breast equilibrium. Comparing agespecific measurements with graphs analyzing the trend of change in growth, the results showed that the change in 1997 was minimal since age 14; however, a continuous increase was achieved in 2013 that corresponded to the age at which growth is complete. The results of these studies are expected to be used as basic data to predict pattern design, body implementation, and trends in shape changes for young women.

Real-Time Polymerase Chain Reaction Assay: A Response to Recent Letter to the Editor

김서우,장정현 대한결핵및호흡기학회 2016 Tuberculosis and Respiratory Diseases Vol.79 No.1

Streptavidin이 융합된 DR4 항원에 특이적인 single-chain Fv 항체의 개발

김서우,우상욱,김진규,Kim, Seo Woo,Wu, Sangwook,Kim, Jin-Kyoo 한국미생물학회 2018 미생물학회지 Vol.54 No.4

Streptavidin (STR)과 Biotin system은 Biotin의 Streptavidin에 대한 높은 비공유 친화력(non-covalent affinity; $K_D=10^{-14}M$)과 4 Biotin 결합부위를 갖는 Streptavidin의 tetramer 구조로 인해 복수의 항원결합부위 및 복수의 항원특이성을 갖는 항체를 제조할 수 있기 때문에 가장 활발하게 연구되고 있다. 이 system을 활용하기 위해 우리는 Streptomyces avidinii 염색체 DNA로부터 PCR을 통해 Streptavidin (STR) 유전자를 증폭하고 이를 TRAIL (tumor necrosis factor ${\alpha}$ related apoptosis induced ligand) receptor인 death receptor 4 (DR4)에 특이적으로 결합하는 hAY4 single-chain Fv 항체유전자에 융합시켰다. 대장균에서 발현시킨 STR에 융합된 hAY4 ScFv (hAY4-STR) 항체는 가열시킨 SDS-PAGE에서 43 kDa monomer를 나타내었다. 그러나 가열하지 않은 SDS-PAGE와 Size-exclusion chromatography에서는 tetramer인 172 kDa을 나타내었는데 이는 hAY4 ScFv-STR 항체가 STR의 자연적인 비공유결합에 의해 유도된 tetramer를 형성하고 있음을 나타내고 있다. 본 융합 단백질은 Ouchterlony assay와 ELISA에서 보여주는 것처럼 자연 Streptavidin과 유사한 Biotin 결합력을 유지하고 있었다. ELISA와 Westernblot을 이용하여 정제된 hAY4-STR 융합항체의 DR4 항원결합력 또한 확인하였다. 게다가 표면 플라즈몬 공명(surface plasmon resonance) 분석에서 hAY4 ScFv-STR tetramer는 tetramerization에 의해 hAY4 ScFv monomer보다 60배 더 높은 항원결합력을 나타내었다. 요약하면 hAY4 ScFv-STR 융합단백질은 E. coli에서 soluble tetramer로 성공적으로 발현 및 정제되었으며 Biotin과 DR4 항원에 동시에 결합함을 보여 주었다. 이는 bifunctional and tetrameric ScFv 항체를 제조 할 수 있음을 제시해 주고 있다. The Streptavidin and Biotin system has been studied most extensively as the high affinity non-covalent binding of Biotin to STR ($K_D=10^{-14}M$) and four Biotin binding sites in tetrameric Streptavidin makes this system useful for the production of multivalent antibody. For the application of this system, we cloned Streptavidin amplified from Streptomyces avidinii chromosome by PCR and fused to gene of hAY4 single-chain Fv antibody specific to death receptor 4 (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand. The hAY4 single-chain Fv antibody fused to Streptavidin expressed in Escherichia coli showed 43 kDa monomer in heated SDS-PAGE. However, this fusion protein shown in both non-heated SDS-PAGE and Size-exclusion chromatography exhibited 172 kDa as a tetramer suggesting that natural tetramerization of Streptavidin by non-covalent association induced hAY4 single-chain Fv tetramerization. This fusion protein retained a Biotin binding activity similar to natural Streptavidin as shown in Ouchterlony assay and ELISA. Death receptor 4 antigen binding activity of purified hAY4 single-chain Fv fused to Streptavidin was also confirmed by ELISA and Westernblot. In addition, surface plasmon resonance analysis showed 60-fold higher antigen binding affinity of the hAY4-STR than monomeric hAY4 ScFv due to tetramerization. In summary, hAY4 single-chain Fv fused to Streptavidin fusion protein was successfully expressed and purified as a soluble tetramer in E. coli and showed both Biotin and DR4 antigen binding activity suggesting possible production of bifunctional and tetrameric ScFv antibody.

Integrating omics analysis of salt stress-responsive genes in rice

김서우,정희정,정기홍 한국유전학회 2015 Genes & Genomics Vol.37 No.8

The detrimental effect of high salinity on crop production is a serious problem. However, the number of genes with known functions relating to salinity tolerance is very limited in rice. To effectively address this limitation, selection of useful candidate genes and identification of major regulatory factors through global approaches are necessary. To this end, we used three data series of affymetrix array data produced with salt-treated samples from NCBI Gene Expression Omnibus (http://www.ncbi.nlm. nih.gov/geo/) and identified 653 rice genes commonly differentially expressed under three salt-stress conditions. While evaluating the quality of selected candidate genes for salt-stress responses, Gene ontology enrichment analysis revealed that responses to salt and water stresses of biological process category are highly overrepresented in salt-stress conditions. In addition, the major salt stress-responsive metabolism process and regulatory gene modules are classified through MapMan analysis, and detailed elements for further studies are suggested. Based on this, we proposed a salt stress-responsive signaling pathway in rice. The functional analysis of the main signal transduction and transcription regulation factors identified in this pathway will shed light on a novel regulatory metabolism process that can be manipulated to develop crops with enhanced salinity tolerance.

The Effectiveness of Real-Time PCR Assay, Compared with Microbiologic Results for the Diagnosis of Pulmonary Tuberculosis

김서우,장중현,김새인,이석정,이진화,류연주,심신애,김유경,이미애,심성신 대한결핵및호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.1

Background: The incidence of tuberculosis (TB) in Korea is relatively high compared to the other Organisation for Economic Co-operation and Development (OECD) countries, with a prevalence of 71 per 100,000 in 2012, although the incidence is declining. Real-time polymerase chain reaction (PCR) has been introduced for the rapid diagnosis of TB. Recently, its advantage lies in higher sensitivity and specificity for the diagnosis of TB. This study evaluated the clinical accuracy of real-time PCR using respiratory specimens in a clinical setting. Methods: Real-time PCR assays using sputum specimens and/or bronchoscopic aspirates from 2,877 subjects were reviewed retrospectively; 2,859 subjects were enrolled. The diagnosis of TB was determined by positive microbiology, pathological findings of TB in the lung and pleura, or clinical suspicion of active TB following anti-TB medication for more than 6 months with a favorable response. Results: Sensitivity, specificity, and accuracy were 44%, 99%, and 86% from sputum, and 65%, 97%, and 87% from bronchoscopic aspirates, respectively. For overall respiratory specimens, sensitivity was 59%, specificity was 98%, and accuracy increased to 89%. Conclusion: Positivity in real-time PCR using any respiratory specimens suggests the possibility of active TB in clinically suspected cases, guiding to start anti-TB medication. Real-time PCR from selective bronchoscopic aspirates enhances the diagnostic yield much more when added to sputum examination.

흡인성 폐렴 환자의 병원 내 사망률과 그 예측 인자

김서우,류연주,홍성철,김경진,정가영,심윤수,이진화,장중현 대한결핵 및 호흡기학회 2011 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.112 No.0

Prevalence and Risk Factors for Reflux Esophagitis in Patients with Chronic Obstructive Pulmonary Disease

김서우,이진화,심윤수,류연주,장중현 대한결핵 및 호흡기학회 2011 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.112 No.-