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한국 자생나리의 기내 인편삽에 의한 Lily Symptomless Virus 제거 및 구근 재배조건에 따른 재감염 분석
김민희(Min Hui Kim),박인숙(In Sook Park),박경일(Kyeung Il Park),오욱(Wook Oh),김규원(Kiu Weon Kim) 한국원예학회 2015 원예과학기술지 Vol.33 No.6
The lily symptomless virus (LSV) is the most common virus in Korean native lilies and causes various types of damage to overall plant growth. This study was carried out to investigate the elimination rate of the LSV by the in vitro scale culture (scaling) method in Korean native lilies and to test reinfection rates of the LSV under several field culture conditions of bulb production. Four Korean native lilies (Lilium dauricum, L. distichum, L. lancifolium, and L. maximowitzii) were used and their scales were cultured in vitro for micro-scale formation. The micro-scales were subcultured repeatedly using MS culture medium supplemented with 30 or 90 g·L<SUP>-1</SUP> sucrose. The culture conditions were 24 μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> PPFD with 16 hour daylength using fluorescent lamps and maintained at 22 ± 2℃. The virus-free bulblets were grown for one to three years in the greenhouse and transplanted to the field in October or March. Virus infection rates were investigated by direct tissue blotting immunobinding assays and measurement of chlorophyll and protein contents. Virus-free plants could be obtained from the 5th subculture of micro-scales in L. lancifolium and L. maximowitzii or from primary culture in L. dauricum and L. distichum. LSV-free plants were reinfected during bulb production in the field. Reinfection rates were higher at older bulb ages and under higher planting density. The plants planted in October and at inland Gyeongsan had higher infection rates than those planted in March and at coastal area Pohang. The reinfection rate of L. maximowitzii was higher than those of L. dauricum and L. lancifolium. The LSV-infected plants had lower chlorophyll contents and unchanged protein contents compared to virus-free plants.
하수오류(何首烏類)의 형태(形態) 감별(鑑別)과 약리연구(藥理硏究)
김민희 ( Min Hui Kim ),서부일 ( Bu Il Seo ) 한약응용학회 2016 한약응용학회지 Vol.16 No.2
Objective : This study presents the basic elements to give an exact discernment to the sorts of Polygonum multiflorum which had been likely to be confused and some characteristic differences, which was done through the examination of pharmacological literature, the classification of the types and the pharmacological study that had already been conducted in Cynanchum wilfordii, Cynanchum auriculatum, Metaplexis japonica and Polygonum multiflorum. Methods : To differentiate the sorts of Polygonum multiflorum in type, based on the classification of the ones of the original plants, I examined their internal and external types, natures, dispositions and medical efficacies. Results : 1. While Cynanchum wilfordii, Cynanchum auriculatum, Metaplexis japonica which belong to Asclepiadaceae have their symmetry leaves, Polygonum multiflorum of Polygonaceae has alternation leaves. While the torn leaves of C. wilfordii and Metaplexis japonica has a resin, C. auriculatum has not. While the three sorts of Metaplexis japonica wind up counterclockwise, Polygonum multiflorum goes up clockwise. While the flowers of the first bloom umbellate or cyme, the flowers of the second bloom panicle, and then their shapes are so different. 2. In the case of cutting the root of Cynanchi Wilfordii Radix we can find a pattern there. On the contrary, there is no pattern in the root of Cynanchi Auriculati Radix When there is a cut, Cynanchi Auriculati Radix has a white resin, but Cynanchi Wilfordii Radix has not. The cutting plane of Polygoni Multiflori Radix has some peculiar pattern. Conclusion : This study was done for the exact discernment and differentiation of the sorts of Cynanchum wilfordii, Cynanchum auriculatum, Metaplexis japonica, Polygonum multiflorum which could have been misused with each other because of their similar types. For the study, We examined the literatures about the classification of their original plants, the differentiation in type and the disposition. As a result, there are remarkable distinctions in the external and internal types and the medical efficacies among them, which could be concluded as they are the different plants each other.
김민희 ( Min Hui Kim ),김평수 ( Pyung Soo Kim ) 한국정보처리학회 2021 정보처리학회논문지. 컴퓨터 및 통신시스템 Vol.10 No.2
In this paper, an alternative finite memory structure(FMS) smoothing filter is developed for discrete-time state-space model with a control input. To obtain the FMS smoothing filter, unbiasedness will be required beforehand in addition to a performance criteria of minimum variance. The FMS smoothing filter is obtained by directly solving an optimization problem with the unbiasedness constraint using only finite measurements and inputs on the most recent window. The proposed FMS smoothing filter is shown to have intrinsic good properties such as deadbeat and time-invariance. In addition, the proposed FMS smoothing filter is shown to be equivalent to existing FMS filters according to the delay length between the measurement and the availability of its estimate. Finally, to verify intrinsic robustness of the proposed FMS smoothing filter, computer simulations are performed for a temporary model uncertainty. Simulation results show that the proposed FMS smoothing filter can be better than the standard FMS filter and Kalman filter.
김민희 ( Min Hee Kim ),조명희 ( Myeong Hui Jo ),유성환 ( Seong Hwan Ryou ),방상영 ( Sang Young Bang ),허정호 ( Jeong Ho Heo ),김현준 ( Hyun Oon Kim ) 한국동물위생학회 2013 한국동물위생학회지 (KOJVS) Vol.36 No.1
We investigated the prevalence of infectious duck diseases using 156 ducks reared in 18 farms of western Gyeong-nam province. As a result, duck viral hepatitis (12.8%), colibacillosis (7.1%), and fungal disease (9.0%) were detected. However, avian influenza and riemerellosis were not detected. During autopsies, we could grossly observed red swollen liver (12.8%), petechial or ecchymotic hemorrhage on liver (11.5%), and fibrinous perihepatitis (9.0%). Gray-white necrotic spot (23.1%), swollen spleen (22.8%), swollen kidney (20.5%), hyperemia or hemorrhage on tracheal mucous membrane (8.3%), and nodule in long or air sac (9.0%) were also found.