젖소 사육에서 탄소 순환 체계에 관한 고찰 연구

김기연,고한종,김치호,최은규,김종구,유영선,김현태,Kim, Ki-Youn,Ko, Han-Jong,Kim, Chi-Ho,Choi, Eun-Gyu,Kim, Joung-Ku,Ryou, Young-Sun,Kim, Hyeon-Tae 한국축산환경학회 2012 축산시설환경학회지 Vol.18 No.1

젖소를 사육하는 유우농가 마을을 대상으로 선행 국내/외 문헌자료들에 대한 고찰을 통해 탄소 유입과 배출에 따른 각 부문별 발생량 원단위에 대한 다음과 같은 결론을 도출하였다. 1. 젖소 사육과정에서 탄소 유입에 대한 발생량 원단위는 젖소의 사료 섭취에 의한 탄소 유입(${\fallingdotseq}$ 5.9 ton C/head/year), 젖소 분뇨 퇴비를 초지로 환원시 탄소 유입(${\fallingdotseq}$ 2.3 ton C/head/year), 초지가 함유하고 있는 유기탄소(${\fallingdotseq}$ 318g C/$m^2$/year), 사료작물이 함유하고 있는 유기탄소(${\fallingdotseq}$ 145 g C/$m^2$/year) 및 광합성에의한 대기 중 $CO_2$의 초지 사료작물 흡수(${\fallingdotseq}$ 17 g C/$m^2$/year)로 정리할 수 있다. 2. 젖소 사육과정에서 탄소 배출에 대한 발생량 원단위는 젖소 호흡 및 트림에 의한 대기 중으로 $CO_2$와 $CH_4$ 배출(${\fallingdotseq}$ 2,9 ton C/head/year), 젖소 분뇨내 유기탄소 분해에 의한 대기 중으로 $CO_2$와 $CH_4$ 배출(${\fallingdotseq}$ 0.4 ton C/head/year), 초지에서 대기 중으로의 $CO_2$ 배출(${\fallingdotseq}$ 440 g C/$m^2$/year), 및 초지 내 유기탄소의 지하수 용출(${\fallingdotseq}$ 0)로 정리 할 수 있다. The first objective of this study is to estimate emission coefficient of organic carbon regarding its inflow and discharge for dairy farm through reviewing domestic and foreign literature published or reported previously. Its second objective is to provide fundamental data to establish carbon cycle system related to livestock production. Based on literature review, emission coefficients by inflow of organic carbon into dairy farm were 5.9 ton C/head/year for feedstuff ingestion by milk cow, 2.3 ton C/head/year for recycling manure compost of milk cow to grassland, 318 g C/$m^2$/year for contents in grassland, 145 g C/$m^2$/year for contents in fodder crop, and 17 g C/$m^2$/year for $CO_2$ uptake by fodder crop, respectively. on the other hand, emission coefficients by discharge of organic carbon from dairy farm were 2,9 ton C/head/year for emission of $CO_2$ and $CH_4$ by respiration and burp of milk cow, 0.4 ton C/head/year for emission of $CO_2$ and $CH_4$ by decomposition of organic carbon in manure of milk cow, 440 g C/$m^2$/year for emission of $CO_2$ from grassland, and 0 for elution of organic carbon in grassland into underground water, respectively.

복합식 공기청정기의 물리적 및 생물학적 입자상 물질의 제거 효과

김기연,김치년,김윤신,노영만,이철민,Kim, Ki-Youn,Kim, Chi-Nyon,Kim, Yoon-Shin,Roh, Young-Man,Lee, Cheol-Min 한국환경보건학회 2006 한국환경보건학회지 Vol.32 No.5

There was no significant difference in the CADR (Clean Air Delivery Rate) between physical aerosols, NaCl and smoke, and biological aerosols, airborne MS2 virus and P. fluorescens, which implicate that the hybrid-type of air purifier, applying the unipolar ion emission and the radiant catalytic ionization, imposed identical reduction effect on both physical aerosol and bioaerosol. Ventilation decreases the efficiency of air cleaning by unipolar ionization because high ventilation diminishes the particle concentration reduction effect. The particle removal efficiency decreases with increase in the chamber volume because of the augmented ion diffusion and higher ion wall loss rate. Particle size affects the efficiency of air ionization. The efficiency is high for particles with very small diameter because reduction of charge increases with particle size. If there is no increasing supply of ions, the efficiency of air cleaning by unipolar ionization increases with respect to initial concentration of particles because of the large space charge effect at high particle concentration and amplified electric field.

담배 배양세포에서 인간 히스톤 단백질 H1.5의 발현

김기연,권석윤,송재영,이행순,곽상수,Kim, Kee-Yeun,Kwon, Suk-Yoon,Song, Jae-Young,Lee, Haeng-Soon,Kwak, Sang-Soo 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.2

Collagen에 의해 유도되는 류마티스 관절염을 저해하는 효과가 있는 인간 histone 단백질 Hl.5 (hHl.5)를 산화스트레스 유도성 SWPA2 프로모터에 연결하여 형질전환 담배(Nicotiana tabacum L. cv Bright Yellow-2) 배양세포주를 개발하였다. hH1.5 유전자는 Agrobacterium 매개 형질전환 방법으로 담배 BY-2 배양세포에 도입되었다. 형질전환 캘러스는 150mg/L kanamycin과 300mg/L claforan이 포함된 변형된 MS 선발배지에서 선발하여, PCR분석으로 hHl.5 유전자의 도입을 확인하였다. 형질전환 현탁배양세포에서 hH1.5 단백질의 발현은 northern 분석과 Western 분석으로 확인하였는데, 담배배양세포에서 재조합hHl.5 단백질 (42 kDa)은 인간의 것 (32 kDa)과는 다른 크기의 단백질이 확인되었다. 금후 재조합 hH1.5 단백질의 자세한 특성규명이 요구된다. Transgenic tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cell lines expressing a human histone H1.5 (referred to as hH1.5), which suppress collagen-induced rheumatoid arthritis, were developed under the oxidative stress-inducible peroxidase (SWPA2) promoter. Tobacco BY-2 cells were transformed by Agrobacterium-mediated method. The kanamycin-resistant calli were selected on the modified MS medium containing 150mg/L kanamycin and 300mg/L claforan. Transgenic cell lines were confirmed by PCR and northern blot analysis. Recombinant hH1.5 (rhH1.5) protein (42 kDa) was also detected by Western blot analysis, showing a different molecular weight of human hH1.5 (32 kDa). These results suggested that a hH1.5 gene was properly introduced in tobacco cultured cells under the control of SWPA2 promoter. The further characterization of rhH1.5 protein remains to be studied.

돈사 작업장 유형에 따른 입자상 오염물질의 실내농도 및 발생량에 관한 현장 조사

김기연,박재범,김치년,이경종,Kim, Ki-Yeon,Park, Jae-Beom,Kim, Chi-Nyon,Lee, Kyung-Jong 대한예방의학회 2005 예방의학회지 Vol.38 No.2

Objectives: Particulate contaminants, such as total and respirable dusts, can harm the health of farm workers via several routes. The principal aims of this field study were to determine the concentrations and emissions of particulate contaminants: total and respirable dusts, in the different types of swine houses used in Korea, and allow objective comparison between Korea and the other countries in terms of swine housing types. Methods: The swine houses investigated in this research were selected with respect to three criteria: the manure removal system, ventilation mode and growth stage of pigs. Measurements of total and respirable dust concentrations and emissions in the swine houses were carried out on 5 housing types at 15 different farm sites per housing type. The swine houses investigated were randomly selected from farms situated within the central districts in Korea: province of Kyung-gi, Chung-buk and Chung-nam. Results: The total and respirable dust concentrations in the swine houses averaged $1.88\;and\;0.64mg/m^3$, ranging from $0.53\;to\;4.37mg/m^3$ and from $0.18\;to\;1.68mg/m^3$, respectively. The highest concentrations of total and respirable dusts were found in the swine houses with deep-litter bed systems: $2.94mg/m^3\;and\;1.14 mg/m^3$, while the lowest concentrations were found in the naturally ventilated buildings with slats: $0.83mg/m^3\;and\;0.24mg/m^3$, respectively (p<0.05). All the swine houses investigated did not exceed the threshold limit values (TLVs) for total ($10mg/m^3$) and respirable ($2.5mg/m^3$) dusts. The mean emissions of total and respirable dusts, per pig (75 kg in terms of live weight) and area ($m^2$), from the swine houses were 97.33 and 9.55 mg/h/pig and $37.14\;and\;12.83mg/h/m^2$, respectively. The swine houses with deep-litter bed systems showed the highest emissions of total and respirable dusts (p<0.05). However, the emissions of total and respirable dusts from the other swine houses were not significantly different (p>0.05). Conclusion: The concentrations and emissions of total and respirable dusts were relatively higher in the swine houses managed with deep-litter bed systems and ventilated naturally of the different swine housing types tested. In further research, more farms than the number used in this research should be investigated, which will present objective and accurate data on the concentrations and emissions of total and respirable dusts in Korean swine houses. In addition, personal sampling should be performed to objectively assess the exposure level of farm workers to particulate contaminants.

서울시 일부 지하철역 내 부유 진균, 입자상 물질, 이산화탄소의 분포 양상

김기연,박재범,김치년,이경종,Kim, Ki-Youn,Park, Jae-Beom,Kim, Chi-Nyon,Lee, Kyung-Jong 대한예방의학회 2006 예방의학회지 Vol.39 No.4

Objectives: The aims of this study were to examine the level of airborne fungi and environmental factors in Seoul metropolitan subway stations and to provide fundamental data to protect the health of subway workers and passengers. Methods: The field survey was performed from November in 2004 to February in 2005. A total 22 subway stations located at Seoul subway lines 1-4 were randomly selected. The measurement points were subway workers' activity areas (station office, bedroom, ticket office and driver's seat) and the passengers' activity areas (station precincts, inside train and platform). Air sampling for collecting airborne fungi was carried out using a one-stage cascade impactor. The PM and CO2 were measured using an electronic direct recorder and detecting tube, respectively. Results: In the activity areas of the subway workers and passengers, the mean concentrations of airborne fungi were relatively higher in the workers' bedroom and station precinct whereas the concentration of particulate matter, $PM_{10}\;and\;PM_{2.5}$, were relatively higher in the platform, inside the train and driver's seat than in the other activity areas. There was no significant difference in the concentration of airborne fungi between the underground and ground activity areas of the subway. The mean $PM_{10}\;and\;PM_{2.5}$ concentration in the platform located at underground was significantly higher than that of the ground (p<0.05). Conclusions: The levels of airborne fungi in the Seoul subway line 1-4 were not serious enough to cause respiratory disease in subway workers and passengers. This indicates that there is little correlation between airborne fungi and particulate matter.

액상 첨가제 살포 방법에 의한 밀폐형 돈사에서의 분진 저감 평가

김기연,고한종,김치년,Kim, K.Y.,Ko, Han-Jong,Kim, C.N. 한국축산환경학회 2008 축산시설환경학회지 Vol.14 No.2

본 연구는 지금까지 활용되었거나 새로이 제안된 액상 첨가제를 돈사에 살포하여 분진 농도의 경시적 변화를 관찰하여 제거 효과에 대해 객관적으로 비교 평가하기 위한 것으로 다음과 같은 결론들을 도출했다. 1. 살포 전 밀폐형 돈사내 분진 농도는 다른 연구자의 결과와 비교시 전반적으로 낮았다. 2. 온도의 경우는 살포 후 24시간까지 돈사 내부와 외부 큰 차이가 없었으나, 상대습도는 살포 후 1시간까지 증가하여 외부와 약 10% 가량의 차이를 보였다. 3. 평가대상 모든 액상 첨가제들의 살포직후 분진 평균 저감율은 살포 전 농도 대비 약 30%로 나타났다(p<0.05). 4. 살포 3시간 이후부터는 콩기름을 제외하고 다른 액상 첨가제들의 분진 농도에 대한 경시적 저감 효과가 관찰되지 않았다. 5. 평가대상 모든 액상 첨가제들 중 콩기름의 살포가 제거 효율성 및 안전성 측면 모두 돈사 분진 제어에 대해 가장 효과적인 첨가제였다. Appropriate air quality in the pig building is essential to prevent farmers' health as well as to increase pig performance. This on-site experiment was conducted to assess dust reduction efficiencies of several liquid additives with spaying method in the enclosed pig building. The mean reduction rate of total dust only after spray was approximately 30% for all the treatments compared to initial level before spraying additives, which was found to reduce the initial level of total dust significantly (p<0.05). The mean reduction rate of all the treatments at 1hr after spray was about 24% which was 6% lower than only after spray. Since 3hr after spray, however, total dust level were fluctuated variably for all the treatments besides application of the soybean oil. Based on this result, effect of soybean oil on dust reduction in the enclosed pig building was most prominent among liquid additives evaluated in terms of efficiency and duration.

Amylase와 Protease의 활성이 높은 현미 발효 미생물의 선별

김기연,김희규,송병철,차창준,Kim Ki-Yeon,Kim Hee-Gyu,Song Byeong-Chul,Cha Chang-Jun 한국미생물학회 2006 미생물학회지 Vol.42 No.2

Brown rice contains rice bran and germ with higher nutritional value and dietary fiber content compared with the polished rice. However, brown rice has a limitation of poor digestion. fermented brown rice could be better nutritional source and improve digestibility. Therefore, we tried to select good fermentative microorganisms which have nutritional values with high amylase and protease activities, and probiotic effects. Nineteen micro-organisms, including eight Bacillus strains isolated from Chongkukjang and 11 lactic acid bacteria, were screened for the fermentation ability and enzyme production. The liquid broths containing 2.5%(w/v) of raw brown rice powder as a sole nutritional source were used for culture media. Among the strains tested, all of the Bacillus strains and two lactic acid bacteria (Leuconostoc gelidum and Pediococcus pentosaceus) showed increase in cell population and enzyme activities. The viable cell counts of all the Bacillus strains and two lactic acid bacteria exceeded $10^7 CFU/mL$. The maximal enzyme activities produced by Bacillus sp. Bl, Bacillus sp. B2, Bacillus sp. B11, L. gelidum and P. pentosaceus were 17.85, 17.50, 17.10, 17.10 and 3.24 U/mL for amylase and 22.48, 22.04, 23.76, 12.13, and 3.4 U/mL for pretense, respectively. Therefore, the results of this study demonstrated that the above strains could be potential starters for the fermentation of raw brown rice.

학술 1 - 기후변화로 인한 젖소에 미치는 영향

김기연,채준석,Kim, Gi-Yeon,Chae, Jun-Seok 대한수의사회 2012 대한수의사회지 Vol.48 No.2

인터넷전화 수용 결정요인과 사용자 연령 및 경험 변수의 조절효과 분석

김기연,이덕선,설정선,이봉규,Kim, Ki-Youn,Lee, Duk-Sun,Seol, Jeong-Seon,Lee, Bong-Gyou 한국정보처리학회 2009 정보처리학회논문지D Vol.16 No.6

본 연구의 목적은 첫째, 인터넷전화(Voice over Internet Protocol)의 사용자 수용에 미치는 결정요인을 정의하고, 둘째, 수용 이론들의 통합모형인 UTAUT를 기저모형으로 하여, 성과기대감, 사회적 영향, 노력기대감, 사용촉진조건, 행위의도와 사용행위 등의 잠재변수 간의 복합적인 인과관계를 검증하는 것이다. 셋째, 비용기대감 변수를 새롭게 정의하여 연구가설에 포함하였고, 각 변수들 간의 경로에 영향을 주는 조절변수로서 사용자 연령, 성별, 경험기간의 조절효과를 분석하였다. 또한, VoIP의 실제 사용자 641명을 표본 조사함으로써, 행위의도와 사용행위 변수 간의 개념적 차이를 명확히 구분하여 보다 정확한 분석 결과의 향상을 도모하였다. 사용자 수용에 대한 기존 연구들은 사용행위 변수를 검증할 때, 실제 사용자뿐만 아니라 긍정적인 행위의도를 가진 미래의 잠재적 사용자를 포함시키는 사례가 많았다. 분석 결과, 제시한 모든 연구가설이 채택되었으며, 조절효과에서 연령이 성과기대감, 사회적 영향과 행동의도 간에, 경험기간은 사용촉진조건, 행위의도와 사용행위 간에 정의 관계가 성립하였다. The purpose of this study is to define determinants of VoIP user acceptance and to verify significant causality among latent variables - performance expectancy, effort expectancy, cost expectancy, social influence, facilitating conditions, behavioral intend, use behavior - based on UTAUT model. We presented the expanded hypotheses including the new factor, cost expectancy and analyzed the moderating effect of user age, gender and usage experience variables. For a accuracy of predicted results, we focused on survey analysis with 641 real user samples. Compared to previous studies, it is meaningful that this research verified the conceptual difference between behavioral intention and usage behavior. As a result, all proposed hypotheses accepted and moderating effects are supported significantly in age and use experience moderating variables.

암환자의 요구 조사 도구 개발

김기연,최상순,박소미,송희영,허혜경,Kim, Gi-Yon,Choi, Sang-Soon,Pak, So-Mi,Song, Hee-Young,Hur, Hea-Kung 한국호스피스완화의료학회 2002 한국호스피스.완화의료학회지 Vol.5 No.2

Purpose : This study was designed to develop an instrument that could be used for comprehensive and effective need assessment for patients with cancer. Methods : In the first phase, a conceptual framework for the instrument was established by Wingate & Lackey (1989). In the second phase, the preliminary instrument was drawn up through a review of the literature and in consultation with three professors in Nursing. In the third phase validity and reliability of the preliminary instrument were tested as follows; 1) an expert validity test of the preliminary instrument was done by nine head nurses and charge nurses who had over ten years experience caring for patients with cancer at Wonju Christian Hospital. 2) A construct validity test and reliability test was done for the instrument by 116 staff nurses selected by convience sampling from hospitals located in Kang-Won, Kyoung-Ki, and Choong-Chung Provinces. The collected data were analyzed using SPSS 10.0 WIN program. For the factors of the instrument, factor analysis was used. The reliability of the scale was analyzed by Cronbach's alpha. Results : The results of the experts' test of validity, showed that, of 32 items, only one item had less than 55.4%. It was then deleted and a total of 31 items was selected. On the basis of the results of the factor analysis, the following six components were identified: physiological, informational, spiritual, and emotional needs, available resources, and legal/financial needs. These factors explained 61.8% of the variance. In the factor analysis, the first factor (physiological needs) and the second factor (informational needs) explained 25.4% and 10.9% of the variance respectively, which were major factors for the needs of patients with cancer in Korea. Cronbach' alpha for the scale was .90 indicating internal reliability. Conclusion : This instrument can be effectively utilized for assessment of needs of patients with cancer in Korea. Use of the needs assessment instrument developed in this study will allow nurses to develop nursing interventions that provide comprehensiveness and continuity in meeting the needs of patients with cancer.