건설업 안전경영시스템모델 개발

채준석,갈원모,손기상 대한안전경영과학회 2001 대한안전경영과학회지 Vol.3 No.2

안전관리 기법에 관한 실증적 연구 - 건설작업을 중심으로 -

채준석,갈원모,손기상 대한안전경영과학회 2000 대한안전경영과학회지 Vol.2 No.1

마필임상(1)-말산업과 유비무환

채준석,Chae, Jun-Seok 대한수의사회 2008 대한수의사회지 Vol.44 No.6

한우에 감염된 Theileria sergenti merozoite의 순수분리와 genomic DNA probe에 관한 연구

채준석,이주묵,권오덕,채건상 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from bovine crythrocytes. The infected erythrocytes were lysed by Aeromonas hydrophila(Ah-1) hemolysin, and the parasites were isolated by ultracentrifugation on a Percoll discontinuous density gradient. For construction of a T sergenti genomic DNA library, T sergenti DNA was digested with PstI and the fragments were ligated into the PstI site of pUC19 before transformation of Escherichia coli JM83. Out of thousands of transformants obtained by transformation of E coli JM83 with the genomic library, three plasmids were chosen. The sizes of the inserted DNAs were 2.9kb(2.4kb and 0.5kb) in pKTS1, 4.3kb in pKTS2 and 1.5kb in pKTS3, respectively. The DNA fragments used as probe KTS1(2.4kb), KTS2(4.3kb) and KTS3(1.5kb) were labeled digoxigenin-11-dUTP for the Southern hybridization. In Southern hybridization, all of the probes(KTS1, KTS2 and KTS3) reacted specifically to T sergenti DNA, but not to bovine leucocyte DNA. In order to find out the sensitivities of the digoxigenin-11-dUTP-labeled KTS1 and KTS3 as the probes, purified merozoite DNA and bovine DNA(control) were checked by dot blot hybridization with the probes. Both of the probes, KTS1 and KTS3, detected as minimum amount of 975pg of the T sergenti DNA, but not bovine DNA even to 500ng.

개의 Babesia gibsoni 감염예방에 관한 연구 1. 항원의 Sonication 및 Formalin 처리에 의한 예방접종

채준석,인동철,이주묵,윤창모,Chae Joon-Seok,Ihn Dong-Cheol,Lee Joo-Muk,Yoon Chang-Mo 한국임상수의학회 1990 한국임상수의학회지 Vol.7 No.1

To examine the effects of vaccination against Babesia gibsoni infection in dogs, 15 normal mixed-breed dogs(5 month to 1 year old) divided into 3 groups with 5 dogs in a group. One of them was selected as control group(group A) and others were selected as experimental groups(group B and C). The group B was vaccinated with sonicated antigens and the group C was vaccinated with 0.2% of formalin treated antigens. The results obtained in the examination were summarized as follows : 1. In the western blot, the lane A revealed specific two bands on the regions of 54kd and 100kd, respectively. 2. After the first vaccination, the antibody titers of group B and C were higher 5 times(1 : 200) than those of control group(1:40). After the second vaccination, the antibody titers of group B and C have not changed. When challenged with the protozoa(Babesia gibsoni), the antibody titers(1 : 5,000) were elevated in all groups. But these were not exceeded over 1 : 5,000 for 4 weeks. 3. After challenge, the peak time of increased numbers of the protozoa was the 15th day (12-18 days) in all groups. During these days, the rate of parasitized erythrocytes in control group was 55.0${\pm}$5.4%. But those of group B and group C were 26.0${\pm}$6.4%, and 15.6${\pm}$7.8%, respectively. 4. After challenge, all of the values of PCV, Hb, RBC were shown to decrease in all of the control and experimental groups. 5. The total leukocytes counts are shown a tendency of reduction in all groups after challenge. 6. In all groups, there were increase in lymphocytes and monocytes after challenge.

안전관리 기법에 관한 실증적 연구 : 건설작업을 중심으로

채준석,갈원모,손기상 안전경영과학회 2000 안전경영과학회지 Vol.2 No.1

Many Safety training up to now has been done at site varying with their condition without any standardization or fundamental work related technique. In order to prevent any accidents from construction site, now as we all know, we have to approach on it with work based method. The authors have investigated a couple of construction site for collecting the ideas from site Engineers of this. Some of ideas analyzed with the above related data have been shown as conclusions.

건설업 안전경영시스템모델 개발

채준석,갈원모,손기상 안전경영과학회 2001 안전경영과학회지 Vol.3 No.2

Safety management cost, US$ one thousand billion has been input to the construction area from 1995 to 2000 but the management quality is still in the developing country level. The above mentioned increase of accident rate are attributed to the generous code regulation, net fit to the sense, varying with time change, but the more fundamental reason of this is no practical construction safety management system, up to now. Hardware-orienting operation for the system could be a main reason of this problem. In this paper, we developed a model for efficient construction safety system. It was found from the case study that the model result in high efficient with low cost.

국내의 병원체, 매개체, 보균동물 및 숙주동물에 있어서 기후변화의 영향

채준석 대한수의학회 2010 대한수의학회 학술대회발표집 Vol.2010 No.-

학술자료 I - 말 원충성척수뇌염

채준석,강준구,Chae, Jun-Seok,Gang, Jun-Gu 대한수의사회 2010 대한수의사회지 Vol.46 No.3

말 원충성척수뇌염(equine protozoal myeloencenphalitis; EPM)은 말에게 심각한 신경성 질병을 일으키는 원충성 질병으로서 Sarcocystis neurona가 원인체이다. 근육포자낭(sarcocyst)을 숨기고 있는 자연중간숙주를 포함한 S. nuerona의 완전한 생활사는 알려져 있지 않으나 주머니쥐의 일종인 opossum(Didelphis virginiana, Didelphis albiventris)이 최종 숙주이며, 말은 정상적 숙주로 알려져 있다. 왜냐하면 말에서는 분열체(schizonts)와 분열소체(merozoites)가 발견되기 때문이다. EPM 유사 질병들이 고양이, 밍크, 너구리, 스컹크, 태평양 점박이 바다표범, 포니, 바다수달 등을 포함한 다양한 포유류에서 발생한다. 고양이는 포자낭(sporocytes) 섭식 후 근육포자낭 단계를 숨기는 실험적인 중간숙주로서 역할을 할 수 있다. 최근 국내에서 신경증상을 나타내는 말에 대한 의심스러운 임상증상이 임상수의사들에 의해 관찰되어졌는, 정확한 진단을 하기위하여 S. nuerona에 의해 발생된 EPM의 역사, 구조, 생활사, 생물학, 병리론, 동물에서 질병 유도, 임상 증상, 진단, 병인론, 생태학, 치료 등에 대하여 알아보고자 한다.

Comparison of Frequency and Stay Time between Normal and Abnormal Elimination Behavior of Cats Using a Litter Box with Automatic Sensor

채준석 한국임상수의학회 2024 한국임상수의학회지 Vol.41 No.2

Changes in elimination behavior, including urination and defecation, are common clinical signs of numerous disorders in cats. Therefore, this study attempted to automatically measure the elimination behavior of cats using the litter box and develop an early warning system for the guardian in case of abnormalities. To construct an early warning system for abnormal changes through cat elimination behavior, it consisted of a litter box, an automatic sensor for data collection and data wifi transmission, a server for data analysis, and a mobile phone app for result transmission and early warning. To establish the reference interval (RI), the elimination behavior was monitored for more than 2 weeks using a motion sensor within a litter box in 37 healthy cats and 19 diseased cats. The data were expressed as daily total visits, daily total stay duration, average stay duration per elimination, weekly total visits, and weekly total stay duration. Healthy cats showed median daily total visits of 3 times/day (RI 1.0-7.0) and daily total stay duration of 192 s/day (RI 8.0-452.0). For weekly data, the median total visits were 20 times/week (RI 3.0-35.25) and the median total stay duration was 1,147 s/week (RI 80.0-2,249.5). The average stay duration per elimination was 59 s/elimination (RI 4.67-132.0). Diseased cats showed more frequent elimination behavior than healthy cats (p < 0.001). Otherwise, for each elimination, diseased cats had shorter stay durations than healthy cats (p < 0.001). This study established the RIs of elimination behavior parameters (frequency and duration) in healthy cats. The present study might help guardians and veterinarians detect changes in elimination behaviors in diseased cats at an early stage.