비타민E 투여가 마우스의 체액성 및 세포성 면역반응에 미치는 영향 -비타민E 결핍환자의 간호중재 개발을 위한 동물실험-

김금재 ( Kim Kum Jae ) 한국간호과학회 1993 Journal of Korean Academy of Nursing Vol.23 No.4

Vitamin E, which has its advocates in the treatment of diabetes melius, autoimmune disease, cancer and peripheral vascular and thromboembolic disease, has now been alleged to have a powerful antioxident effect and to affect various biological activities such as fertility factor, inhibition of human platelet aggregation and stabilization of biological membranes. The present study was designed to test whether vitamin E(alpha-tocopherol) can : (1) enhance the hemagglutinin response to sheep red blood cells (SRBC), (2) modulate Arthus and delayed type hypersensitivity(DTH) to SRBC and contact hypersensitivity to dinitrofluorobenzene (DNFB), (3) enhance the mitogenic response of murine splenocyte, (4) decrease the recovery of Cryptococcus neoformans from brain, lung, liver, spleen and kidney of infected mice and (5) have an inhibitory or enhancing effect on the induction of active systemic anaphylaxis (ASA) induced by chicken-gamma globulin (CGG) in mice. Mice were given either intramuscular injections of 0.3ml (300mg) of vitamin E before immunization or were infection for 10 consecutive days or were given by vitamin E esophageal intubation, 0.1ml(100mg), for 20 days before sacrifice for the mitogenic response experiments. It was found that vitamin E treated mice showed a significant enhancement in hemagglutinin response, Arthus reaction and DTH to SRBC and contact hypersensitivity to DNFB. There was no significant difference in the mitogenic response to phytohemagglutinini PHA), but the response to concanavalin A(ConA) or pokeweed mitogem(PWM) was increased in vitamin E-treated mice. Interestingly, the vitamin E administration before C. neoformans infection decreased significantly the recovery of C. neoformans from brain, lung, liver, spleen and kidney of the infected mice as compared with that of the control mice, strongly suggesting that vitamin E pretreatment may increase the resistance of mice to the fungal infection. Unexpectedly, vitamin E administration enhanced the production of CGG - induced ASA. Taken together, it can be concluded that vitamin E administration may increase the humoral and cellular immune response and resistance to C. neoformans infection, but enhance the induction of ASA to CGG. Further studies are necessary to clarify the underlying mechanism accounting for these effects.

통닭튀김 산패유를 이용한 버섯균사체의 생산

정기태,김규태,최정식,홍재식,김금재 ( Gi Tai Jung,Kyu Tae Kim,Joung Sik Choi,Jai Sik Hong,Kum Jae Kim ) 한국균학회 1996 韓國菌學會誌 Vol.24 No.4

단전호흡술이 혈당치에 미치는 효과

이경완(Kyeong Wan Lee),변주나(Ju na Byun),김금재(Kum Jae Kim) 대한스트레스학회 2000 스트레스硏究 Vol.8 No.1

韓國人의 Toxoplasma Gondii 抗體價

金錦才 全北大學校 醫科大學附設 看護專門大學 1981 논문집 Vol.7 No.-

In order to determine the incidence of toxoplasma antibodies in Korean people, 72 medical and 166 nursing students were examined by Sabin-Feldman dye-test. Out of a total of 238 human sera from the Jeonbug University medical and nursing students, the overall positive rate was 41.6%. The difference in positive rate was observed between male and female, showing 33.3% and 45.2% respectively. There was a tendency that the dye-test titer of female is somewhat higher than that of male (medical students). The mode of transmission of the parasite to human was briefly discussed.

간호 학생의 개인 위생의 실천에 관한 조사 연구

김금재 全北大學校 醫科大學附設 看護專門大學 1982 논문집 Vol.8 No.-

This research paper on practice of personal hygiene of nursing college students in Jeonju was made by sampling randomly 345 1st, 2nd and 3rd grade students of the Nursing College in Jeonju from the 28th of april to the 18th of may 1981. The results are summarized into 13 items as follows : 1. The house-holders were farmers, foresters, fishermen and related workers(39.7%), salesmen (23.9%), civil servants (18.8%) and other workers (10.1%). 2. Economic situation of the respondants : ordinary (78.6%), less than ordinary (15.4%), and more than ordinary (2.3%). 3. Frequencies of brushing their teeth:tiwce(69.9%), once (26.1%), and three times a day (4.0%) 4. a) Habit of washing their hands before meals : regularly (37.7%), sometimes (51.9%), never (10.4%) b) Habit of washing their hands after having been to the toilet : regularly (42.3%), sometimes (34%), never (10.4%) 5. Taking a bath : once a week (45.5%), once every two weeks (43.2%), and twice a week (7.2%) 6. Frequencies of changing under-wear : once every two days (35.9%), twice per week (35.7%), daily (19.2%) 7. Answers to the question : "What kind of water do you drink?" Any kind of water (60%), always boiled water (30.4%), sometimes not boiled water (9.6%) 8. Percentage of the student's knowledge about hygiene : some knowledge (53.9%), extensive knowledge (45.2%), no knowledge (0.9%). 9. Informations related to personal hygiene was received from : the teachers (53.6%), from news papers and/or magazines (18.6%), form the parents (9.3%). 10. Their parents and/or brothers and sisters(61.5%) were reported as the main health consultants. Others were medical doctors (18.2%), nurses (9.3%) and pharmacists (5.2%). 11. Medical care was mostly received from pharmacies (63.5%), hospitals or private clinics(22.9%), 10.7% had their medical care at home. 12. The practical extent of the personal hygiene appeared as follows : moderate (56.2%), more than ordinary (30.4%), and less than ordinary (4.1%). 13. Reasons why they do not practice personal hygiene is the lack of hygienic facilities because of : lack of money (41.7%), indolence (22.3%), and bad habits (21.7%).

간호대학생의 월경 주기 동안의 기초체온 변화에 관한 조사 연구

김금재,이숙희 전북대학교 의과학연구소 1986 全北醫大論文集 Vol.10 No.3

This study was undertaken to investigate the menstrual cycles and ovulation times. The investigation was made among 100 students of the Department of Nursing during a period of three months from May to July, 1984. The ovulation time was determined by measuring basal body temperature. It was found that the menstrual cycles ranged from 20 to 34 days, and that the most common cycle was 26 to 34 days(89%). The average cycle was 30.8 days. Average basal body temperature was the lowest at 15 days prior to expected flow, regardless of menstrual cycles. The basal body temperature dropped gradually during the pre-ovulatory phase and it gradually rose again during the post-ovulatory phase. These results suggest that there may be a relationship between the lowest besal body temperature and the ovulation time.

右側重複尿管과 開口異常을 同伴한 尿管奇型 1例와 看護

金錦才 全北大學校 醫科大學附設 看護專門大學 1980 논문집 Vol.6 No.-

One Case of ureteral malformation of right duplicated ureter associated with single ectopic ureteral orifice to the vaginal vestibule in a 14-years old girl was presented. In a case while normal self-voiding urinary leakage has been complained since infant without other urinary symptoms and she was treated with right ureteroneocystostomy in paquin's method.

正常月經週期동안의 末梢血液 T-淋巴球의 變動

金錦才,鄭憲鐸,河大有 大韓免疫學會 1980 大韓免疫學會誌 Vol.2 No.1

This study was undertaken to measure peripheral blood T-lymphocyte during the various phases .of the normal menstrual cycle and to investigate the circadian variations of peripheral blood leukocytes. The experiment was carried out on three medical students in the dark-room under the light-dark regimen of L. D. 17: 7 for circadian rhythm and on fifteen nursing students under the normal life pattern for lunadian rhythm. A significant decrease in T -lymphocytes occurred during. the menstrual period. However, the percentages of T -lymphocytes returned to the pre-levels one week after the end. of the menstrual period. On the other hand, total numbers of lymphocytes and neutrophils showed ' no changes during the menstrual period. The acrophase of peripheral neutrophil count was in the early stage of activity span whereas the acrophases of peripheral eosinophil count and lymphocyte count were in the midstage of the rest span.

비타민 E 투여가 Cryptococcus neoformans 및 Candida albicans 감염에 대한 마우스의 저항에 미치는 영향 : 진균감염증 환자의 간호중재 개발을 위한 동물실험 Animal experiment for the development of nursing care for fungal infected patients

김금재 中央醫學社 1996 中央醫學 Vol.61 No.8

The previous studies found that vitamin E(VE) administration increases the humoral and cellular immune response in mice. The present study was undertaken to investigate to know the effects of VE administration on the resistance of mice to two commensal yeasts, Cryptococcus neoformans and Candida albicans which cause opportunistic infections in immunocompromised or severeiy debilitated hosts. Mice were daily treated with intramuscular injection of 300 mg/mouse of VE on 10 consecetive days before intravenous inoculation of 5 X 105 CFU of C. neoformans or C albicans. The colony forming units(CFU) of C neoformans in the lungs, brains, livers, spleens and kidneys of VE-treated and control mice were enumerated 9 days after the infection. VE administration significantly reduced the number of C. neoformans recovered from the above organs examined. Furtheremore, VE adnninistration enhanced survival of mice that had been experimentally infected with C. neoformans compared with control mice. VE administration also reduced the numbers of C. albicans recovered from kidneys of experimentally infected mice. The effects of VE administration on delayed-type hypersensitivity reaction to cryptococcal culture filtrate(CneF) antigen was also assessed and it was found that VE administration enhanced the delayed-type hypersensitivity reaction to CneF. Taken together, the present data strongly suggested that VE administration may increase the resistance to infections with C. neoformans or C. albicans.

단식과 Cyclophosphamide가 먼역반응에 미치는 영향에 관한 실험적 연구

김금재 中央醫學社 1992 中央醫學 Vol.57 No.3

It is important to know the correlation between food restriction and immune functions. However, very little research has been conducted on the effect of food restriction on immune functions and the underlying mechanism to account for the immunological alterations induced by limit-feeding is still not clear. In an attempt to understand better the effects of food restriction stress on immune response and to clarify such mechanism, the effect of food deprivation or water deprivation on cellular and humoral immune responses and the effect of cyclophosphamide (CY), a widely used antitumor drug, on the immunological alterations induced by fasting were studied. The cellular immune response was evaluated by delayedtype hypersensitivity (DTH) reactions to sheep red blood cells (SRBC) and contact hypersensitivity to dinitrofluorobenzene (DNFB). The humoral immune response was assessed by hemagglutinin response to SRBC. Mice were stressed by deprivation of food or water for 2 days before immunization. CY was given intraperitoneally as a single dose of 250 mg/Kg 2 days before immunization. The control mice were fed ad libitum both pellet and water. It was found that food deprivation for 2 days led to enhance Arthus reactions and 24-hr DTH reactions to SRBC. CY, given to food-deprived mice enhanced contact hypersensitivity to DNFB as well as Arthus and DTH reactions to SRBC. The water deprivation for 2 days also enhanced significantly the Arthus and DTH reactions. In contrast to cellular responses, food deprivation decreased hemagglutinin response, and the depressed antibody response by food deprivation stress was significantly reserved by CY injection. Taken together, these findings suggest that food restriction may enhance cell-mediated immunity but suppress antibody response in the applied experimental conditions and that CY-sensitive suppressor T cells may play an important role in the immunological alterations induced by stress.