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흑염소 간-조직과 Ferrocene 매개체를 사용한 과산화수소정량 전류법 바이오센서
권효식,박인근,김양숙,Kwon, Hyo-Shik,Park, In-Keun,Kim, Yang-Sug 대한화학회 2004 대한화학회지 Vol.48 No.5
흑염소의 간-조직과 ferrocene을 탄소반죽 전극에 고정하여 과산화수소를 정량할 수 있는 전극을 만들었다. Ag/AgCl 전극에 대하여 $-0.3{\sim}+0.0\;V$의 전위 범위에서 전극의 감도를 관찰하였으며 전극의 감응시간은 12 s로 나타났다. 전극의 검출한계는 2.25${\times)10^{-6}M$ (S/N=3)이었으며,1.0${\times}10^{-2 }$M 과산화수소를 이용해 15회 반복 측정한 결과, 상대표준편차는 1.87%이었다. 또한, 방해물질의 영향도 없는 것으로 나타났다. 효소전극의 감도는 19일 사용 후 50%로 감소하였다. The response characteristics of the bioelectrode developed by the co-immobilization of black goat liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined ($-0.3{\sim}+0.0\;V$ vs. Ag/AgCl), the response time was relatively short (t95%=12 s) and it responded in the wide range of pH. The detection limit was 2.25${\times)10^{-6}M$ and a relative standard deviation of the measurements which were repeated 15 times using 1.0${\times}10^{-2 }$M hydrogen peroxide was 1.87%. The bioelectrode sensitivity decreased to 50% of the original value in 19 days of continuous use.
구름 버섯(Coriolus versicolor) 탄소반죽 전극을 이용한 과산화 수소 정량 바이오센서
권효식 ( Hyo Shik Kwon ) 충북대학교 과학교육연구소 2012 과학교육연구논총 Vol.27 No.2
The response characteristics of the new biosensor developed by the co-immobilization of juice of Coriolus versicolor mushroom and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined(-0.20 - 0.00 V vs. Ag/AgCl), it responded in the wide range of pH and its biocatalytic stability was superior. Also, it``s detection limit was 1.75×10-6 M ( S/N = 3) and it had relatively good selectivity to the materials considered to disturb the response. The result of this biosensor study using juice of Coriolus versicolor mushroom can be utilized as the basic information for the determination of hydrogen peroxide.
권효식 ( Hyo Shik Kwon ),김두영 ( Doo Young Kim ) 충북대학교 과학교육연구소 2007 과학교육연구논총 Vol.23 No.1
The continuous increase of the world population is escalating the requirements for energy and materials. Increasing science industrial and technological development have additional needs energy and raw materials. The fossil energy and nuclear energy research and development has been declining since the late 1980s, for the higher energy price, pollution and the shortage of energy resources. The world needs clean, abundant, and cheap sources of energy to sustain itself. So we need the energy research and development for the economical renewable and alternative energy. In this paper, we review the present energy resources(conventional energy) and the energy futures(renewable and alternative energy).