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귀밑샘 암종에서 생존 예측을 위한 임상병리 인자 분석 및 머신러닝 모델의 구축
곽승민(Seung Min Kwak),김세헌(Se-Heon Kim),최은창(Eun Chang Choi),임재열(Jae-Yol Lim),고윤우(Yoon Woo Koh),박영민(Young Min Park) 대한두경부종양학회 2022 대한두경부 종양학회지 Vol.38 No.1
Background/Objectives: This study analyzed the prognostic significance of clinico-pathologic factors including comprehensive nodal factors in parotid gland cancers (PGCs) patients and constructed a survival prediction model for PGCs patients using machine learning techniques. Materials & Methods: A total of 131 PGCs patients were enrolled in the study. Results: There were 19 cases (14.5%) of lymph nodes (LNs) at the lower neck level and 43 cases (32.8%) involved multiple level LNs metastases. There were 2 cases (1.5%) of metastases to the contralateral LNs. Intraparotid LNs metastasis was observed in 6 cases (4.6%) and extranodal extension (ENE) findings were observed in 35 cases (26.7%). Lymphovascular invasion (LVI) and perineural invasion findings were observed in 42 cases (32.1%) and 49 cases (37.4%), respectively. Machine learning prediction models were constructed using clinico-pathologic factors including comprehensive nodal factors and Decision Tree and Stacking model showed the highest accuracy at 74% and 70% for predicting patient’s survival. Conclusion: Lower level LNs metastasis and LNR have important prognostic significance for predicting disease recurrence and survival in PGCs patients. These two factors were used as important features for constructing machine learning prediction model. Our machine learning model could predict PGCs patient’s survival with a considerable level of accuracy.
소세포폐암에서 Neuron Specific Enolase의 면역조직 화학염색과 혈청농도에 관한 연구
곽승민 ( Seung Min Kwak ),김형중 ( Hyung Jung Kim ),신동환 ( Kong Hwan Shin ),장중현 ( Joong Hyun Jang ),이홍렬 ( Hong Lyeol Lee ),김세규 ( Se Kyu Kim ),안철민 ( Chul Min Ahn ),김성규 ( Sung Kyu Kim ),이원영 ( Won Young Lee ),이 대한결핵 및 호흡기학회 1992 Tuberculosis and Respiratory Diseases Vol.39 No.6
조철호,곽승민,문태훈,조재화,류정선,이홍렬,Cho, Chul-Ho,Kwak, Seung-Min,Moon, Tae-Hun,Cho, Jae-Hwa,Ryu, Jeong-Seon,Lee, Hyong-Lyeol 대한결핵및호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.47 No.5
연구배경: 세포성장은 세포증식과 세포죽음의 균형에 의해 이루어 진다. 세포성장에 관여하는 여러 growth factor증 IGF-I은 IGF-IR와 결합하여 세포증식을 유발하는 mitogen으로 알려져 있다. 또한 IGF-I에 결합하는 IGFBPs중에 IGFBP-3는 혈액내에 가장 많은 carrier protein으로, IGF-I과 결합하여 IGF-I의 세포증식 효과를 증가 혹은 억제시킨다. 방 법: 3T3 fibroblast 세포를 이용하여 IGF-I과 IGF-IR transcripts를 northern blot으로 확인하고, IGF-I에 의한 mitogenic effect를 MTT assay 및 $^3H$-thymidine incorporation test로 관찰하고, IGF-I의 receptor인 IGF-IR의 활성화를 보기 위해 intracellular $\beta$-subunit의 tyrosine kinase domain의 phosphorylation을 western blot으로 관찰하였다. 또한 IGFBP-3가 3T3 세포에서 mitogenic effect에 미치는 영향을 보기 위해 anti-IGFBP-3와 ${\alpha}IR_3$을 단독 및 병용투여하여 관찰하였다. 결 과: 3T3 세포는 IGF-I와 IGF-IR의 mRNA expression을 보였으며, IGF-I을 투여시 IGF-IR의 intracelluar cytoplasmic protein인 $\beta$-subunit의 tyrosine kinase domain을 phosphorylation시켜 활성화시키며, 5%, 1% serum-containing media에서 세포증식을 보였으나, serum-free media에서는 세포증식을 보이지 않았다. 또한 anti-IGFBP-3 투여와 ${\alpha}IR_3$과 anti-IGFBP-3를 병용투여시는 세포종식이 각각 2배이상 증가하였으나, ${\alpha}IR_3$을 4시간 전처치후 ${\alpha}IR_3$과 anti-IGFBP-3를 병용투여시는 anti-IGFBP-3에 의한 세포종식이 보이지 않은 것으로 보아 IGF -I/IGFBP-3 결합에서 분리되는 free IGF-I어l 의해 세포증식이 유도된 것으로 생각된다. 결 론: IGF-I은 IGF-IR를 phosphorylation시켜 mitogenic effect를 보이며, IGFBP-3는 IGF-I의 mitogenic effect를 억제하는 것으로 생각된다. Background: Cell growth is a balance between cell proliferation and cell death. Insulin-like growth factor-I(IGF-I), which binds IGF-I receptor(IGF-IR), mediates cellular proliferation as a potent mitogen. IGF binding protein-3(IGFBP-3) as a circulating major IGFBP can inhibit or enhance the effects of IGF-I on cellular growth by binding IGFs. Methods: We investigated the expressions of mRNA of IGF-I and IGF-IR by northern blot and phosphorylation of IGF-IR with the treatment of IGF-I by western blot in 3T3 fibroblast cells. The cellular proliferations of 3T3 cells with the treatments of IGF-I were evaluated using $^3H$-thymidine incorporation and MTT assay. Also to observe the effect of IGFBP-3 on cellular proliferation, 3T3 cells were treated with anti-IGFBP-3 and ${\alpha}IR_3$(monoclonal antibody to IGF-IR) alone or in combination. Results: Our results demonstrated that 3T3 cells showed mRNA expressions of IGF-I and IGF-IR and the IGF-I increased phosphorylation of IGF-IR. The treatments of 3T3 cells with IGF-I increased cellular proliferation in 5 % and 1 % seruma-containing media, not in serum-free media. The addition of anti-IGFBP-3 to neutralize IGFBP-3 showed 2-fold increase of cellular proliferation, and also co-incubation of anti-IGFBP-3 and ${\alpha}IR_3$ together showed similar increase of cellular proliferation in 3T3 cells. Interestingly, when the cells were pretreated with ${\alpha}IR_3$ for 4 hr, prior to the simultaneous addition of ${\alpha}IR_3$ and anti-IGFBP-3, anti-IGFBP-3-mediated cellular proliferation was decreased to control level. All of these results suggest that free IGF-I released from IGF-I/IGFBP-3 complex would be involved in the cellular proliferation. Conclusion: IGF-I is a mitogen through the activation of IGF-IR in 3T3 cells, and IGFBP-3 could be a potent inhibitor for IGF-I action by binding IGF-I.
조직 미세배열법을 이용한 비소세포 폐암 조직에서 에스트로겐과 프로게스테론 수용체 발현
한혜승 ( Hye Seung Han ),김민지 ( Min Ji Kim ),조재화 ( Jae Hwa Cho ),윤용한 ( Yong Han Yoon ),곽승민 ( Seung Min Kwak ),이홍렬 ( Hong Lyeol Lee ),김광호 ( Kwang Ho Kim ),류정선 ( Jeong Seon Ryu ) 대한결핵 및 호흡기학회 2005 Tuberculosis and Respiratory Diseases Vol.58 No.1
연구 배경 : 비소세포 폐암의 암화 과정에서 에스트로겐과 프로제스테론 단백의 역할에 대한 면역조직화학 염색을 이용한 연구들이 진행 중이다. 그러나 이 연구들은 아직 일치된 결과를 보이고 있지 않으며 이는 상용하는 면역조직화학 염색법이 한 문제로 제시되고 있다. 저자 들은 최근 새로 개발된 조직미세배열법을 이용하여 비소세포 폐암 환자의 조직에서 이들 호르몬 수용체 발현을 연구하였다. 대상 및 방법 : 대상은 70예의 비소세포 폐암 환자로 남성이 74%, Background : To evaluate the role of estrogen and progesterone in the carcinogenesis of NSCLC, IHC studies for the expression of the receptors of estrogen and progesterone have been performed with inconsistent results. Recently the TMA method has been dev
우리나라 성인 남성에서 흡연량과 공복혈당장애 또는 제2형 당뇨병 발생과의 연관성
박창해,가혁,임종환,곽승민,김환철,최지호,Park, Chang-Hae,Ga, Hyuk,Lee, Jong-Han,Kwak, Seung-Min,Kim, Hwan-Cheol,Choi, Ji-Ho 대한예방의학회 2008 예방의학회지 Vol.41 No.4
Objectives: To investigate whether smoking and the smoking status are predictors of the incident impaired fasting glucose (IFG) or type 2 diabetes in Korean men. Methods: A cohort of 1,717 Korean men without IFG or diabetes, who underwent annual periodic health examinations for 4 years (2002-2006), were retrospectively investigated. IFG and diabetes were defined as a serum fasting glucose concentration of 100-125 mg/dL and more than 126 mg/dL, respectively. Cox's proportional hazards model was used to evaluate the association between smoking and development of IFG or type 2 diabetes. Results: A total of 558 cases (32.5%) of incident IFG and 50 cases (2.9%) of diabetes occurred. After controlling for the potential predictors of diabetes, the relative risk for IFG, compared with the never smokers, was 1.02 (95% CI=0.88 to 1.19) for the ever-smokers, 0.96 (95% CI=0.79-1.16) for those who smoked 1-9 cigarettes/d, 1.15 (95% CI=1.01 to 1.30) for those who smoked 10-19 cigarettes/d, and 1.31 (95% CI=1.10 to 1.57) for those who smoked 20 or more cigarettes/d (the P value for the current smokers was only p<0.002). The respective multivariate adjusted relative risks for type 2 diabetes, compared with the never-smokers, were 1.07 (95% CI=0.64 to 1.92), 1.47 (95% CI=0.71 to 3.04), 1.84 (95% CI=0.92-3.04), and 1.87 (95% CI=1.13-3.67), respectively (the P value for the current smokers was only p=0.004). Conclusions: The smoking status and the number of cigarettes smoked daily are associated with an increased risk for developing IFG or type 2 diabetes in Korean men.
소방이 형성된 결핵성 흉막염 환자에서 경피적 도관을 이용한 유로키나제 치료의 효과; 전향적 무작위연구
이용환 ( Yong Whan Lee ),곽승민 ( Seung Min Kwak ),권미영 ( Mee Young Kwon ),배인영 ( In Young Bae ),박찬섭 ( Chan Sup Park ),문태훈 ( Tae Hun Moon ),조재화 ( Jae Hwa Cho ),류정선 ( Jeong Seon Ryu ),이흥렬 ( Hyong Lyeol Lee ),노 대한결핵 및 호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.47 No.5
결핵성 및 암성 흉수의 감별진단에서 흉수내 SC5b - 9 농도 측정의 진단적 의의
서동완(Dong Wan Seo),곽승민(Seung Min Kwak),김우성(Woo Sung Kim),김동순(Dong Soon Kim),서을주(Eul Ju Seo),민원기(Won Ki Min),김원동(Won Dong Kim),고윤석(Youn Suk Koh) 대한내과학회 1994 대한내과학회지 Vol.47 No.6
N/A To evaluate the diagnotic value of pleural SCSb-9 in the differential diagnosis of tuberculous and malignant pleural effusion, we measured pleural ADA activity and SCSb-9 concentration in 71 cases of pleural effusions due to tuberculosis (n=35) and malignancy (n=36). And we also measured pleural C4d fragment and Bb fragment concentration to determine by which pathway the complement activation occurs in pleural space. The results were as follows: 1) The median value of pleural SC5b-9 concentration of tuberculous effusion was 6.4 ug/ml (interquartile range 2.6~11.7) and was significantly higher than that of malignant effusion (p<0.005). 2) The median values of pleural C4d fragment concentration were 15.5 mg/ml (9.9~18.9) in tuberculous effusion and 12.8 mg/ml (7.3~19.6) in malignant effusion. There were no significant difference between two groups. The median value of pleural Bb fragment concentration of tuberculous effusion was 7.8 mg/ml (5.5~12.8) and was significantly higher than that of malignant effusion (p<0.01). 3) The SC5b-9 concentration showed no significant correlation with C4d fragment concentration both in tuberculous effusion and malignant effusion. But the SC5b-9 concentration showed significant correlation with Bb fragment concentration both in tuberculous effusion (r=0.48, p<0.005) and malignant effusion (r=0.72, p<0.001). 4) The median value of pleural ADA activity of tuberculous effusion was 64.3 U/L (40.9~103.5) and significantly higher than that of malignant effusion (p<0.001). 5) Pleural ADA activity showed significant correlation with pleural SC5b-9 concentration in tuberculous effusion (r=0.53, p<0.005). 6) According to the receiver-operating characteristic curve analysis, the measurement of pleural ADA activity is superior to that of pleural SC5b-9 concentration in the differential diagnosis of tuberculous and malignant effusion. In conclusion, the measurement of pleural SC5b-9 concentration can be used as an adjunctive test in the differential diagnosis of tuberculous and malignant effusion but pleural ADA activity measurement was superior to pleural SC5b-9 concentration measurement. And pleural SC5b-9 concentration of tuberculous effusion was higher than that of malignant effusion and it seems to be due to the difference in activation of alternative pathway activation of complement in two groups.