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- 저자 : 韓盛植 (검색결과 208 건)
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한성식 한국곤충학회 1983 Korean journal of entomology Vol.13 No.2
월동중인 배추흰나비 번데기의 지방체를 재료로하여 -3$0^{\circ}C$에서 1hr동안 급속동결 처리후 지방세포가 받는 냉해를 전자현미경을 사용하여 관찰한 결과 다음과 같은 결과를 얻었다. 1. 급속 냉처리 에서 가장 큰 변화를 받는 기관은 핵으로서 Chromatin들이 파괴되어 nuclear membrane에 집적되어 있었다. 핵의 모양 역시 대조군에 비하여 불규칙 적으로 변화하였다. 2. 급속 냉 처리시 proton storage granule에서 granule membrane은 유리되었고 P 5.g은 파괴되었다. 3. Lipid droplet는 변화가 없었다. 4. mitochondria, rough endoplasmic reticulum등 cell organelles은 냉해를 받지 않았다. 솔잎혹파리 유충의 지방세포의 냉해를 전자현미경을 통하여 관찰한 결과 다음과 같은 사실들을 확인하였다. 1 냉 처리시 지방세포가 전반적으로 전자밀도가 높아진다. 2. 핵에서도 degradation이 일어났으며 특히 단백질 저장과립 (protein storage granule)은 가장 뚜렷하게 냉해를 보여주었다. 3. Glycogen particles이나 lipid droplets들은 냉처리시에 아무런 변화가 일어나지 않았다. A ultrastructural study on fat body cells of Pieris rapae L. and Thecodiplosis japonensis Uchida et Inouye was made to observe their fine structures of normal hibernation and freezing injury at -3$0^{\circ}C$. The results were as follows: In Pieris rapae L 1 Nucleus suffered freesing injury most by rapid freezing, where chromatins were degraded and accumulated around nuclear membrane. 2. Protein strage granules were degraded by rapid freezing. 3. Mitochondria, rough endoplasmic reticulum and other cell organelles did not changed at -3$0^{\circ}C$. In Thecodiplosis japcnensis Uchida et Inouye 1. The electron density of fat body cells, on the wholly, increased when they were frozen at -3$0^{\circ}C$. 2. Degradation was found in nucleus and protein storage granule, especially, soured freezing injury. 3. Glycogen particles and lipid droplets did not changed when they were exposed to rapid freeging at -3$0^{\circ}C$.
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곡식얼룩명나방(Plodia interpunctella Hubner)의 Sex Pheromone Gland에 관한 형태학적 연구
한성식,남상호,김우갑,김창환 한국곤충학회 1979 Korean journal of entomology Vol.9 No.2
Female Plodia interpuctella Hubner was cheesed as a material and its sex pheromone glands were observed by light microscope and electron microscope. 1. In morphology Pheromone gland cells were various from the cuboid, around 3 micron in length, to column, around 14 micron in length, and these cells were arranged with monolayer. 2. Lumen wall was covered with the bilayered cuticle in accordance with the staining degree. 3. Starting from apodeme 6 to 6.3 micron in diameter, the pheromone gland was connected with both sides of abdomen muscle. 4. Pheromone gland between 8th and 9th segments was inveginated in the 8th segment, and judging from its total view its shape was like "ring". Especially in the lower side it was considerably deeply rooted with an estimated sixty micron in length in comparision with the other parts. 5. Cell cytoplasm of pheromone gland connected with the cuticle layer showed the infolded phenomenon thoroughgoingly. 6. Cytoplasm had well-developed smooth endoplasmic reticulum, but few rough endoplasmic reticulum, and a few ribosomes, sometimes forming clutters 7. The phenomena of mitochondrial autolysis took place, accassionally, microtubule was well developed.
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배추흰나비 (Pieris rapae L.)와 누에나방(Bombyx mori L.) 의 변태(變態)에 따른 지방체(脂肪體)의 미세구조(微細構造) 변화(變化)에 관한 연구(硏究)
한성식,김지현,김창환,김우갑,Han, S.S.,Kim, J.H.,Kim, C.W.,Kim, W.K. 한국현미경학회 1982 Applied microscopy Vol.12 No.2
The fat bodies of cabbage worm (Pieris rapae) and silk worm (Bomyx mori) during metamorphosis was comparatively studied by electron microscope. 1. Cell oranelles: Golgi apparatus were not observed in both species. It is observed that RER of cabbage worms initiate to degenerate in prepupa stage with complete degeneration at adult stage, while that of silk worms shows similar degenerative pattern. However, mitochondria of cabbage worms are transformed into autophagic vacuole from prepupa stage until adult stage whereas those of silk worm shows a decrease in number in prepupa stage but maintains a certain level until adult stage. 2. Storage substance in cell: Lipid droplets in cabbage worms were observed to increase in numbers during larval stage but afterward decrease in number with an enlargement in size. However immediately after their pupal stage, they almost disappear. On the contrary lipid droplets in silk worms show rather increase in number until adult stage. Protein storage granules in bothspecies were arised from autophagic vacuoles(lysosome) . Fat cells of cabbage worm in adult stage turn out to be residual bodies which last until final stage, but those of silk worm rapidly decrease. Glycogen particles in both species reach maximum at last larval instar and thee gradually decrease thereafter. 3. Fat body sheath: The average width of fat body sheath was measured to be $0.2{\mu}m$ and $0.6{\mu}m$ and surface of fat cells adjacent to fat body sheath in silk worm is heavily infolded.
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배추흰나비 (Pieris rapae L.)의 성숙란에 관한 전자현미경적 연구
한성식,김지현,이근옥,김우갑,김창환 한국곤충학회 1980 Korean journal of entomology Vol.10 No.1
배추흰나비 암컷의 ovariole내에 있는 성숙된 oocyte와 산란후 부화되기전까지의 embryo를 전자현미경으로 관찰하였다. 1. 산란전의 oocyte cortex finger-like projection이 발달되어 있으나 산란되면 없어지며 ovariole sheath를 형성하는 cell과 chorion 사이에는 무수히 많은 microvilli가 있다. 2. yolk protein 형성은 Golgi complex, E.R에서 site가 제공되는 것으로 생각되었다. 3. 본종에서 oocyte에 glycogen particle이 존재하였다. 4. 산란후 초기 배발생이 시작되면 oocyte periplasm에 있던 소기관은 흩어져 각기 group으로 존재하였다. 5. blastodermal cell은 매우 미분화된 상태를 보여주었다. The matured oocyte in the ovariole of Pieris rapae L. and the embryo before the hatching from the breeding were observed. 1. The oocyte cortex before breeding has many finger-like projections but after the breeding the projection was disappeared and numberless microvilli existed between the tell forming ovariole sheath and chorion. 2. The formation of yolk protein took place in the site of Golgi complex and E.R. 3. Glycogen particle existed in the oocyte of Pieris rapae L. 4. On the beginning of the development, microorgans were in the existence of islet form. 5. Blastodermal cells were on the remarkably undifferentiated condition
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