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임상병리 진단검사에서의 차세대염기서열분석 기술의 적용 및 발전 방향
김현숙 청주대학교 보건의료과학연구소 2019 보건의료과학연구 Vol.8 No.1
The Sanger Sequencing method, a sequencing technique of DNA that includes genetic information from living organisms, is the most popular sequencing method. However, because this method has consumed high cost and time, Next Generation Sequencing(NGS) technique has been developed to supplement this method. With the development of the NGS technique, various disease-related genes can be simultaneously analyzed from a single sample, and investigation and molecular diagnostics using genome analysis have been started in earnest. For the first time in 2013, Illumina's MiSeqDx platform and in 2014 Thermo's Ion PGM Dx system was approved by the US FDA for diagnosis. After that, researches on cancer gene discovery, cancer development research, various cancer diagnosis research and biomarker development are actively being carried out. Accordingly, major overseas companies are developing NGS platform for diagnosis and NGS has started to be applied in various medical institutions in Korea. NGS technology is expected to be applicable to the diagnostic laboratory medicine, genome research, population genetics, microorganism species identification and metagenomics, forensic DNA profiling, and industrial process. Therefore, in the future, NGS panel of various diseases will be developed and it is predicted that it will play a critial role in the development of precision medicine which is receiving the highest attention am ong medical fields.
Effects of Ginsenosides Rg3 and Rh2 on the Proliferation of Prostate Cancer Cells
김현숙,이은희,고성룡,최강주,박종희,임동순 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.4
Ginseng has an anti-cancer effect in several cancer models. This study was to characterize active constituents of ginseng and their effects on proliferation of prostate cancer cell lines, LNCaP and PC3. Cell proliferation was measured by [3H]thymidine incorporation, the intracellular calcium concentration by a dual-wavelength spectrophotometer system, effects on mitogen- activated protein (MAP) kinases by Western blotting, and cell attachment and morphologic changes were observed under a microscope. Among 11 ginsenosides tested, ginsenosides Rg3 and Rh2 inhibited the proliferation of prostate cancer cells. EC50s of Rg3 and Rh2 on PC3 cells were 8.4 mM and 5.5 mM, respectively, and 14.1 mM and 4.4 mM on LNCaP cells, respectively. Both ginsenosides induced cell detachment and modulated three modules of MAP kinases activities differently in LNCaP and PC3 cells. These results suggest that ginsenosides Rg3 and Rh2-induced cell detachment and inhibition of the proliferation of prostate cancer cells may be associated with modulation of three modules of MAP kinases.