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삽사리의 가계 분석에 사용할 수 있는 DNA probe 의 선정
정운복,하지홍,이호섭,김종봉,이상로,탁연빈,박희천 한국유전학회 1991 Genes & Genomics Vol.13 No.4
In order to breed the Korean native dog, sapsaree, which exist as a single population in KyungPook province, we are going to use DNA fingerprinting as a tool for studying the pedigree. In this experiment, 4 kinds of DNA probes, Exon 8, Intron B, M13, and pV47-2 were analysed by means of conventional Southern hybridization with Sapsaree genomic DNAs digested with restriction enzymes for their usefulness as probe. On the basis of their autoradiographic band patterns and resolution, pV47-2 was determined to be the best probe for studing the pedigree of sapsaree.
유방 침윤성 관암종의 CD44 발현도와 예후 인자들과의 관계
김정완,곽정식,손윤경,이승철,정운복 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1
CD44 is a family of transmembrane glycoproteins involved in cell-cell and cell-matrix interactions. Expression of CD44 isoforms (splice variants) has been shown to be associated with poor prognosis in several human cancers. We evaluated the expression patterns of the CD44 isoform (CD 44 splice variant v6) in infiltrating ductal carcinoma of the breast by immunohistochemical and RT-PCR method. Paraffin embedded blocks from seventy-five cases of mastectomized samples were analyzed immunohistochemically using monoclonoal antibody against CD44v6. CD44v6 was detected in fifty-seven cases(76%) of the tumor samples. Adjacent normal myoepithelial cells and ductal epithelial cells revealed focal positive reaction to CD44v6. Thirty-two cases (80.0%) withe lymph nodal metastasis revealed overexpression of CD44v6 monoclonal antibody, but twenty-five cases (71.4%) without nodal metastasis also showed positive reaction to CD44v6 monclonal antibody, and there is no statisticallly significant value. Other prognostic factors of infiltrating ductal carcinoma, such as tumor size, histologic grade and hormonal receptors did not show any significant correlation with CD44v6 expression. The RT-PCR studies for 9 cases of infiltrating ductal carcinoma showed the same band patterns both in the normal and tumor tissues. From the above results, it is concluded that the expression of CD44v6 is not a valuable prognostic marker of infiltrating ductal carcinoma of breast.(Korean J Pathol 1997;31: 635~643)
간세포암종에서 TPEF의 Methylation에 의한 불활성화
정운복,김순영,전소영,강구성,이해암,김정옥,박지영,손윤경,김정완 대한병리학회 2008 Journal of Pathology and Translational Medicine Vol.42 No.1
Background : Abnormalities of genomic methylation patterns have been shown to play a role in the development of carcinoma, and the silencing of tumor suppressor genes is related to local de novo methylation. Methods : Using methylation specific arbitrarily primed-Polymerase Chain Reaction (Ms AP-PCR), we identified a 322 bp sequence that contained a 5’ un-translated and exon1 regions of the TPEF gene. To evaluate the inactivation of the TPEF gene through hypermethylation in hepatocellular carcinoma (HCC), we investigated the correlation between methylation patterns and TPEF expression in tumor tissues of human HCC and cell lines via a Combined Bisulfite Restriction Assay (CoBRA) and RT-PCR. Results : A dense methylation pattern of the TPEF was detected in most cell lines, as well as in 10 of the 14 (71.4%) HCC tissues. In addition, loss of heterozygosity (LOH) from the TPEF gene was observed in 5 of the 14 (36%) HCC tissues. Furthermore, RT-PCR analysis revealed TPEF expression in 5 of 8 (62.5%) cell lines. Finally, treatment with a demethylating agent, 5-Aza- 2’-deoxycitidine (5-AzaC), increased the expression of TPEF mRNA. Conclusion : These results indicate that inactivation of the TPEF gene through hypermethylation may be a mechanism by which tumorigenesis occurs in HCC.
정운복,하지홍,김종봉,이상로,탁연빈 한국유전학회 1991 Genes & Genomics Vol.13 No.4
The Sapsaree is a nearly extincted Korean native dog whose historic habitat has included much of the Southeastern Korea. The only existing population of Sapsaree consists of 50 dogs living in the small area of Kyung-San Goon in Kyung-Pook Province. Field observations of the population indicated the presence of two distinct morphological phenotypes that are divided into Blue- and Yellow-Sapsaree. A RFLP (Restriction Fragment Length Polymorphism) analysis using pV47-2 probe and Sapsaree DNA indicated the existance of two distint genetic stocks concordant with the morphological phenotypes.
하지홍,정운복,이상로,탁연빈,김종봉 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.1991 No.1
The Sapsaree is a nearly extincted Korean native dog whose historic habitat has included much of the Southeastern Korea. The only existing population of Sapsaree consists of 50 dogs living in the small area of Kyung-San Goon in Kyung-Pook Province. Field observations of the population indicated the presence of two distinct morphological phenotypes that are divided into Blue- and Yellow-Sapsaree. A RFLP (Restriction Fragment Length Polymorphism) analysis using pV 47-2 probe and Sapsaree DNA indicated the existence of two distint genetic stocks concordant with the morphological phenotypes.
내시경 생검으로 얻은 위선암조직에서의 Microsatellite 불안정성 분석
최병렬,김진아,정운복,홍수형,김정완,이창형,손윤경,조창호,배정동,김호각,양전호 대한소화기학회 2000 대한소화기학회지 Vol.35 No.5
Background/Aims : Microsatellite instability (MSI) is a genetic alteration observed frequently in gastric carcinoma (GC). To assess the role of microsatellite instability (MSI) in gastric cancer (GC), we investigated the incidence of MSI, especially widespread MSI (MSI-H). Methods : Endoscopic biopsy specimens from 59 gastric adenocarcinomas were analyzed for MSI at 13 loci. Paraffin-embedded GC samples from the same 59 GC were analyzed for p53 protein overexpression using immunohistochemical staining. Results: MSI was detected in 33/59 (56%). MSI-H in which over than 40% of investigated loci had MSI, was observed in 7 cases (12%) and low MSI (MSI-L), in which less than 40% of the loci had MSI, was observed in 26 cases (44%). All MSI-H found in 7 cases (12%) were located in distal stomach. There was no difference in the incidences of MSI and MSI-H according to the histology of Lauren's classification, TNM stage, presence of metastasis to lymph node and family history of GC. Overexpression of p53 protein was detected in 43/59 (73%) GC, but MSI-H was not related to the p53 overexpression. MSI in interferon-αR was observed more frequently in GC with family history than GC without family history (55% vs 3%)(p=0.0002). Conclusions : MSI and p53 mutation are involved frequently in oncogenesis of GC. MSI-H is frequently observed in distal GC, but is not related with tumor progression and family history of GC.
삽사리의 가계 분석에 사용할수 있는 DNA probe의 선정
이상로,이호섭,정운복,탁연빈,박희천,김종봉,하지홍 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.1991 No.1
DNA지문법은 범죄감식, 친자감별, 가계분석, twin zygosity의 결정, 집단 유전학, 행동 생태학 등의 분야(jeffreys et al., 1985:Gill et al., 1985, 1987 : Lander, 1989:Neufeld and Colman, 1990)에서 널리 활용될 뿐만 아니라 멸종위기에 처한 동식물의 육종과 보존을 위해서도 없어서는 안되는 중요한 연구방법이 되었다.(Georges et al., 1988:Burke et al., 1989). 최근 미국에서는 멸종위기에 처한 퓨마와 매의 보존을 위해 이들 동물들에 대한 RFLP연구를 행했는데 현존집단내 혈통관계에 대한 중요한 정보를 얻을 수 있었다