Ba(La<SUB>1/2</SUB>Nb<SUB>1/2</SUB>)O₃-Pb(Zr, Ti)O₃ 세라믹의 광변조 특성에 관한연구

류기원(柳基元) 대한전기학회 1994 전기의 세계 Vol.43 No.12

사매가 위암세포에 미치는 영향

류봉하,김진성,윤상협,류기원,홍상선,Ryu Bong-Ha,Kim Jin-Sung,Yoon Sang-Hyub,Ryu Ki-Won,Hong Sang-Sun 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Background : Nowadays many researches about it s cure are going on world widely since cancer is one of the most human health threatening diseases. In Chinese and North Korean medicine, Duchesnea india(Audra.) Foche. is practically used to treat many kinds of cancer, but in Korea it is rarely used. So, we need to scientifically identify anti-tumor effects of Duchesnea india(Audra.) Foche. Objective : We are aimed to identify anti-tumor effects of Duchesnea india(Audra.) Foche. on the stomach cancer cells through molecular biologic methods. Material & Methods : We used AGS as stomach cancer cells from American Type Culture Collection. We added the boiled extract of Duchesnea india(Audra.) Foche. $5{\mu}l$(Sample I), $10{\mu}l$(Sample II) to cultural media(ml)for 0,6, 12, 24, 48 hours. We measured the killing effect on stomach cancer cells through Tryphan blue exclusion test and the suppressive effect on viability of stomach cancer cells via MTT assay. the quantitative RT-PCR was used to examine their effect on the revelation of Bcl-2, Bcl-XL, and Bax, which are genes related to apoptosis. We measured change of mitochondria membrane permeability and membrane potential via flow cytometry. Result : 1. The killing effect on stomach cancer cells showed that each test groups killed more stomach cancer cells than the control group with a time(6 hours later) and density dependent manner, which was statistical significance. 2. The suppressive effect on viability of stomach cancer cells showed that each test groups had more suppressive effects on viability of stomach cancer cells than the control group with a time(6 hours later), which was statistical significance. 3. In the test about the revelation of genes related to apoptosis, the revelation of Bcl-2 and Bcl-XL decreased with a density manner which was statistical significance. but the revelation of Bax was not changed with statistical significance. 4. As a result of this test, Duchesnea india(Audra.) Foche. caused apoptosis by decreasing the absorbance of mitochondria with statistical significance. and also induced apoptosis by decreasing the membrane potential of mitochondria. Conclusion : This experiment showed that Duchesnea india(Audra.) Foche. has anti-tumor effect with statistical significance. This is in vitro experiment and basic experiment on Duchesnea india(Audra.) Foche. We hope more progressive researchs on Duchesnea india(Audra.) Foche. will go on and its anti-tumor effects will be more practically identified.

Ba(La<SUB>1/2</SUB>Nb<SUB>1/2</SUB>)O₃의 고용량과 Zr/Ti 조성비가 Ba(La<SUB>1/2</SUB>Nb<SUB>1/2</SUB>) O₃-Pb(Zr,Ti)O₃ 세라믹의 전기광학 특성에 미치는 영향

柳基元(Ki-Won Ryu),李成甲(Sung-Gap Lee),裵善基(Seon-Gi Bae),李永熙(Young-Hie Lee) 대한전기학회 1995 전기학회논문지 Vol.44 No.5

기능성 소화불량증 환자에 대한 반하사심탕 엑기스제의 임상효과

류봉하,류기원,김진성,윤상협,Ryu Bong-Ha,Ryu Ki-Won,Kim Jin-Sung,Yoon Sang-Hyub 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Objective : We evaluated the therapeutic effect of Banwhasasimtang dry extract powder on gastrointestinal symptoms and its side effects in patients with functional dyspepsia. Methods : Patients with functional dyspepsia, defined as discomfort in the epigastrium. A total 45 patients participated in this study. Dyspeptic symptoms ratio was obtained by patients, total point of symptom score/total 21 points x 100(%). On each of symptom after 4 weeks administration of Banwhasashim-tang was made by variation of symptom score: If symptoms score of posttreatment is lower than that of pretreatment, it is improved ; Otherwise aggravated, and if there is no change between them, unchangeable. When a full health condition of normal control is assumed 100%, relative health condition of dyspeptic patient could be calculated from 'full health condition-dyspeptic symptoms ratio'. Therefore a whole satisfaction rate on symptom improvement was presented with variation of a relative health condition to dyspepsia between pretreatment and post. Results : Banwhasashimtang significantly improved symptoms in patients. The significance was shown in both all seven items(p<0.01, respectively) and 19.2% (p<0.01) of increase of a whole satisfaction rate on symptom improvement. There is no remarkable side-effect in all laboratory test, excepting 1 case of abdominal pain and another one case of recurred pain of chronic prostatitis. Conclusion : Banwhasashimtang extract provides symptomatic relief in functional dyspepsia and is no remarkable side-effect drug.

21세기의 새로운 疾病觀과 한방임상의 現代化에 대한 提言

柳基遠 대한한의학회 1992 대한한의학회지 Vol.13 No.2

難治病에 대한 韓醫學的 治療의 展望

柳基遠 대한한방종양학회 1996 대한한방종양학회지 Vol.2 No.1

단삼보혈탕(丹蔘補血湯)이 Stress로 유발(誘發)된 백단삼보혈탕(白丹蔘補血湯)이 Stress로 유발(誘發)된 백서(白鼠) 위장관(胃腸管)의 조직손상(組織損傷)에 미치는 영향(影響)

류봉하,박동원,류기원,공현식,Ryu, Bong-Ha,Park, Dong-Won,Ryu, Ki-Won,Kong, Hyun-Sig 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.1

Objectives : This is the experimental paper to study the curative and preventive effects of Dansambohyultang, Methods : Oriental herb medicine for gastro-intestinal disease caused by stress, on the rats put under the stress through starvation, heating and immoblization, the author used four different group; the normal group, which was not put under stress and well fed, and the control group, to which the drug was not administered and put under the stress through starvation, heating and immoblization, the Sample I, to which the drug was administered before they were put under stress, the Sample lI, to which the drug was administered after they were put under stress. Results : When a Masson's trichrome stain method was applied to the control group, a small size of ulcer was found in the fundus and the pylorus, and atrophy was observed in the neck region of mucous membrane. When the drug was administered to the Sample I and the Sample II, the former recovered from the ulcer and atrophy to almost normal and showed better results than the latter. When a Masson's trichrome stain method was applied to the control group, atrophy of mucous membrane was found all over the intestines. When the drug was administered to the Sample I and the Sample ll, the curative and recovered effects was seen in both, but the former showed better results than the latter. After application of Mayer's hematoxylin stain method, the observation of numerical changes of goblet cells in the small intestines showed the most significant decrease in the control group. In the Sample I, the number of the cells in the duodenum, the jejunum and the ileum returned to an almost normal level. In the Sample II, a slight increase was observed only in the ileum. After application of Mayer's hematoxylin stain method, the observation of numerical changes of goblet cell in the large intestines showed a significant decreased in the control group. Both the Sample I and the Sample II showed an increase in the number of goblet cells in comparison with the control group. When a serotonin-immunoreaction test was applied, the control group showed a general decrease in the number of gastro-entero-endocrine cells, The Sample I showed the effect in all over gastro.intestinal tract as compared with control group and the Sample II showed the effect in all over gastro-intestinal tract except the duodenum and the former showed better results than the latter. Conclusion : According to the above results, the regions which are most sensitive to stress were observed to the ileum and the colon. It was concluded that Dansambohyultang has a remedical value particularly on the abnormalities caused by stress in all over gastro-intestinal tract and it was found to be more effective in the Sample I than in the Sample ll. Judging from the fact that the Sample I showed better results than the Sample ll, Dansambohyultang can be said to have a preventive effect more than a curative effect on gastro-intestinal tract disease.

보심건비탕(補心健脾湯)과 정전가미이진탕(正傳加味二陳湯)의 적출장기(摘出臟器), 항궤양(抗潰瘍), 위액(胃液)·gastrin 분비(分泌), 장관수송능(腸管輸送能) 및 진통(鎭痛).진경작용(鎭痙作用)에 대한 비교(比較) 연구(硏究)

류봉하,박동원,류기원,윤상건,김진성,Ryu, Bong-Ha,Park, Dong-Won,Ryu, Ki-Won,Yin, Chang-Jina,Kim, Jin-Seong 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.1

Objectives : This is the experimetal paper to evaluate and compare the effects of Bosimgunbitang(補心健脾湯) with those of Jungjungamiyijintang(正傳加味二陳湯) on contraction of isolated organs, anti-ulcer, secretion of gastric juice, secretion of gastrin in serum, transport ability of intestine, analgesic effect and sedative effect Methods : We used mice and rats administered with the extract of the above herbs. Results: Bosimgunbitang and jungjungamiijintang showed the inhibitory effect on the smooth muscle contraction of the isolated ileum induced by acethylcholine chloride and barium chloride in mice. transport ability of intestine. Bosimgunbitang and jungjungamiijintang showed the inhibitory effect on the contraction of the fundus-strip induced by acethylcholine chloride and barium chloride in rats. The preventive effect on pyloric ulcer, indomethacin induced ulcer and ethanol-HCl induced ulcer bf mice was significant. The inhibitory effect of Bosimgunbitang on gastric free acidity, total acidity in Shay rats was significant. The inhibitory effect of on gastrin secretion in serum was significant after only 1 hour when the Bosimgunbitang and jungjungamiijintang was administered. The promoting effect of the jungjungamiijintang on the transport ability of small intestine was significant in the high concentration. The promoting effect of the Bosimgunbitang and jungjungamiijintang on the transport ability of large intestine was significant. The analgesic and the sedative effect were recognized. It meaned that Bosimgunbitang and jungjungamiijintang effected on the C.N.S.. Conclusion : Bosimgunbitang and jungjungamiijintang have the inhibitory effect on the smooth muscle abnormal contraction of the isolated ileum and the anti-ulcer effect to prevent, secretion of gastric juice and gastrin in serum. And they also have to increase a transport ability of intestine, analgesic effect and sedative effect. Jungjungamiijintang especially is eminent for analgesic effect while Bosimgunbitang has excellent sedative effect.

사군자탕가산자고가 위암세포에 미치는 항암효과에 대한 분자생물학적 연구

류봉하,류기원,윤상협,김진성,김진석,Ryu, Bong-Ha,Ryu, Ki-Won,Yoon, Sang-Hyub,Kim, Jin-Seong,Kim, Jin-Seok 대한한방내과학회 2002 大韓韓方內科學會誌 Vol.23 No.2

1. Background The previous studies on anticancer medicine derived from korean traditional medicine have focused on the life elongation of cancer cell bearing animals. However, it is thought that more molecular biological studies are needed to reveal their mechanism. 2. Objective The aim of this study was to investigate the molecular biological function of Sagunjatang plus Cremastrae Appenediculatae Tuber on cytostaticity, apoptosis and apoptosis related genes revelation against human stomach cancer cells(AGS). 3. Methods After administrating Sagunjatang and Sagunjatang plus Cremastrae Appenediculatae Tuber to human stomach cancer cell. MTT assay was performed to compare and examine the efficacy of each medicine on the cytostaticity of stomach cancer cells in proportion to time and doses, and apoptosis assay was performed to examine their effect on apoptosis by using DAPI dye and counting the number of cells which developed in an apoptotic body. In addition, the quantitative RT-PCR was used to examine their effect on the revelation of Bcl-2, Bax and P53, which are genes related to apoptosis. 4. Result and Conclusion Sagunjatang plus Cremastrae Appenediculatae Tuber demonstrated increased cytostaticity. decreased apoptosis and unremarkable revelation of apoptosis related genes. But in the cytostaticity and apoptosis, Sagunjatang plus Cremastrae Appenediculatae Tuber showed a tendency to control stomach cancer cells. Therefore, we can expect the clinical application to the related diseases. Besides, it needs another experiment on various cancer cells, such as, lung cancer cell and hysterocarcinoma cell.

대규모 인터넷 시뮬레이션을 위한 네트워크 토폴로지 자동 생성 기법

류기원 ( Key-won Ryu ),최경희 ( Kyung-hee Choi ),박승규 ( Seung-kyu Park ),정기현 ( Gi-hyun Jung ),이상훈 ( Sang-hun Lee ),박응기 ( Eung-ki Park ) 한국정보처리학회 2005 한국정보처리학회 학술대회논문집 Vol.12 No.2

인터넷 토폴로지 생성 기법에 대하여 많은 연구가 진행되고 있다. 이는 인터넷 토폴로지가 라우팅, 멀티캐스트, 프로토콜 등의 성능과 사이버 테러 공격에 대한 분석 및 방어체계와 밀접한 관계가 있기 때문이다. 토폴로지를 생성하는 GT-ITM[5], INET-3.0[6] 등은 Autonomous System (AS) 노드들로 이루어진 네트워크 토폴로지를 생성한다. 즉, 이들 도구들은 AS 가 노드인 토폴로지를 생성하며, AS 내의 네트워크에 대해서는 표현하지 않는다. 이에, 본 논문에서는 대규모 네트워크의 시뮬레이션을 위한 토폴로지를 구성하는 데 필요한 PoP (Presence of Point), ISP(Internet Service Provider) 등 하위 레벨의 토폴로지를 자동 생성함으로써 네트워크 토폴로지를 표현하는 방법을 제안한다. 본 연구에서 제안하는 방법은 서로 다른 특성을 가진 하부 서브넷을 가지고 있으며 hierarchy 를 반영한 토폴로지를 생성할 수 있다는 장점을 가지고 있다. .