B형 간염바이러스의 태아와 성인간세포에 대한 감염성 해명 연구

정태호,김성국,서화정,윤영국,임종완,차상호 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

사람의 태아와 성인의 간세포에 대한 B형 간염바이러스의 감염도를 비교 분석함으로써 HBV 와 간세포간의 친화도내지는 HBV 수용체에 관한 정보를 얻고자 함 대상 및 방법 : 성인이 외상을 입어 부득이 손상된 간조직을 절제하였을 경우와 모자보건법상 임신지속이 어려운 상태에서 부인과적 수술시 21주 전후의 태아 간조직을 얻어 DMEM 을 배지로 50% 용량으로 HBsAg 양성인 사람 혈청을 가하여 5일간 체외 배양하고 병리조직학적 처리후 효소 면역학적 방법으로 HBsAg 를 염색하여 바이러스의 존재 여부를 검색하였다. 결과 : 태아와 성인 간조직을 5일간 체외 배양하였을 때 그 조직상은 간조직임을 인지할 수 있었다. HBV 의 감염여부는 DAKO 사의 효소면역 법으로 조사한 결과 태아의 간조직에서는 HBsAg 양성부위를 발견하지 못하였으나, 성인 간조직에서는 HBsAg 양성부위가 발견되었다. 결론 : 태생 21주 전후의 태아의 간세포는 HBV 와 친화도는 아주 미약하거나 없고, 성인 간세포는 HBV에 대한 친화도가 높다. 따라서 태생 21주의 간세포에는 HBV 의 감염이 어렵다는 것을 인지하게 되었다. Considerable progress has been made in the immune prophylaxis of hepatitis B virus(HBV) infection and in the understanding of its replication and pathogenecity at the molecular level. However, a convenient in vitro assay for HBV infectivity has been lacking and the early steps of viral life cycle are now yet well understood. Primary cultured human hepatocytes are reported to be susceptible to HBV 10-12, but these systems are not practical because they are short-lived and require primary explanted human liver. However, it is uncertain yet whether primary hepatocytes of human fetal liver are susceptible to HBV for infection or not. We have expanded the infectivity of HBV into a primary cultures of human fetal hepatocytes together with controversy of adult primary hepatocytes. To investigate the infectivity of HBV for, adult and fetal hepatocytes After 5 day culturing fetal(1) and adult(2) liver tissues, which obtained from a surgical operation on DMEM culture media with half HBV positive serum, separating liver cells and fixing them, the existence of HBsAg antigen was investigated through the immuno-enzymatic staining. HBsAg stained area was not found in the 21 weeks gestation liver cells until 5 days of invitro-culture, while it was found in the adult liver cells. This test result showed that the HBV infection is very hard to happen in the liver cells 21 weeks gestation or prior one.

An Integrated Tin Oxide Gas Sensor on Silicon Substrate

鄭完永 世明大學校 1992 世明論叢 Vol.2 No.-

도시가스로 널리 사용되고 있는 LPG를 모니터링하기 위해 실리콘기술을 이용한 SnO₂가스센서를 제조하였다. 이 가스센서는 실리콘 IC기술과 SnO2 박막형성법을 결합하여 제작되었다. 9개의 센서들(3x3 에레이)로 구성된 전체적인 칩크기는 9x9mm였다. 각 센서는 열손실의 감소와 이웃 기판물질로의 열전달을 방지하기 위하여 SiO₂/Si₃N₄/SiO₂층의 얇은 막위에 형성되었다. SnO₂막은 열증착 방법으로 금속 Sn박막을 형성한후 600℃에서 열산화하여 형성하였다. SnO₂(PT)막을 만들기 위해 수십A 의Pt 층을 SnO₂막 위에 스퍼터링하고 막의 전기적 특성의 안정화를 위해 500℃에서 수시간 열처리하였다. 제조된 SnO₂(Pt) 마이크로센서는 동작온도 250℃에서 LPG의 주요성분인 5000ppm 의CH₄와C₄H₁에 대해 각각 85%와 92%의 감도를 나타내었고 5초 이하의 빠른 응답특성을 보였다.

船舶抵當權法制에 관한 考察

鄭完溶 圓光大學校 1989 論文集 Vol.23 No.1

In order to purchase existing vessels or other the building of new vessels, it is necessary for finance to be freely available. To encourage the provision of finance, a method has been developed in most national systems of assuring financiers that their loans are protected. Mortgages(at Common law) and hypotheques(in civil law) are designed to provide security to the lender of money(the mortgagee) in that he has the right to satisfy his claim with priority over most other claimants against the vessel and his security remains intact even if the mortgaged vessel is transferred to new ownership. The right of the mortgagee is enforced through the sale of the mortgaged vessel. A person wishing to purchase an existing vessel or order the building of a new vessel(the mort-gagor) will borrow the purchase money from a bank or other financial institution(the mortgagee). An agreement will be made between these two parties as to the repayment of the loan capital and other matters such as the amount of interest on the loan, insurances required and events causing the repayment of the loan. The mortgage agreement gives to the mortgagee the vessel as security which can be realized by arrest and sale in the event that the loan is put in jeopardy. It is possible to transfer ownership of a mortgaged vessel but the mortgage remains attached to the vessel. Although the mortgagee acquires "property" in the vessel, he is not entitled to make use of it so long as the mortgagor is not in default;his "property" ceases automatically and reverts to the mortgagor if the latter has performed all the obligations which are assumed by the mortgagee, otherwise the mort-gagee's right as a proprietor gets effect although only temporarily and to a restricted extent. In civil law systems the method differs:in the case of non-performance of the borrower's obligations, the person entitled may not take possession of the vessel but must apply to the court or to a suitable judicial authority as being competent for enforced sale in order to be satisfied from the proceeds of the enforced sale. The legal institution of hypotheque maritime upon a vessel follows the model of land own-ership. Private landed property can be used as security for the landowner's debt or that of a third person in whose favour the hypotheque has heen granted. Most national systems provide for the recognition of mortgages only when these have been recorded in a national register.

천연가스 누설 감시시스템 개발

정완영 東西大學校 1999 동서논문집 Vol.5 No.-

천연가스(도시가스) 누설 모니터링 시스템을 개발하였다. 미세분말의 SnO₂분말을 합성하고 그것을 이용한 스크린 인쇄방법에 의해 후막형 반도체 가스센서를 제조하였다. 제조된 센서소자는 천연가스배관 보호용철판에 부착되어서, 각각의 센서소자로부터의 데이터를 국부적인 모니터링 시스템에서 모으게 하였다. 본 연구에서 제조된 가스누설 모니터링시스템은 개발된 시스템의 가능성을 알아보기 위하여 우선 4-채널로 구성되었다. 제조된 시스템은 실제 지하 환경에서 잘 동작 하였으며, 이 결과는 이러한 시스템이 저비용의 다채널 모니터링시스템으로서, 천연가스파이프 네트웍의 누설감시시스템으로 사용될수 있음을 나타내어 주고 있다. A natural gas(or city gas) leakage monitoring system was developed. Semiconducting SnO₂gas sensor was fabricated by thick film screen printing method using synthesized nanocrystalline power. Fabricated sensor elements were attached on safeguard steel plate of natural gas supply pipes, and the local monitoring system get together the data of each sensing elements. The fabricated leakage monitoring system get together the data of each sensing elements. The fabricated leakage monitoring system in this study included 4 channels to know the feasibility of the developed system. The fabricated system was well operated under practical underground circumstance, so this kind of system could be applicable to leakage monitoring system of natural gas pipe network for simultaneous multi-channel monitoring at low cost.

오존감지를 위한 졸-겔 In₂O₃박막의 형성

정완영 東西大學校 2000 동서논문집 Vol.6 No.-

Ozone sensing properties of In2O3 semiconductor thin film prepared by sol-gel method have been investigated. The indium alkoxide precursor was synthesized from the reaction involving indium hydroxide and butanol. The indium sol solution was deposited by spin coating technique. The In2O3 thin films were coated between 1 and 5 times followed by drying at 100℃ and calcining at 600℃. The film thickness was controlled by the number of spin coating. The morphology and the thickness of the In2O3 films were examined by SEM and XRD. The In2O3 thin films have show high sensitive to ozone gas at 250℃ temperature. The In2O3 sensor showed very good selectivity to CH4, CO and C4H10 gases.

마이크로 가스센서의 열해석

정완영,이상윤,임준우,이덕동 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

Thermal analyses for typical stack-type and newly proposed planar-type micro-gas sensors were accomplished. The thermal analyses for the proposed planar structure including temperature distribution over the sensing layer and power consumption of the heater were carried by finite element method and well compared with those of typical stack-type micro-gas sensor. The thermal properties of the microsensor from thermal simulation were tested to investigate the applicability of the heater to a real device.

전기광학 소자를 이용한 광응용 전압센서에 관한 연구

김영수,정진완,전병실 전북대학교 전자산업개발연구소 1991 전자산업연구 Vol.2 No.-

醱酵飼料 제조에 관한 硏究 : 3. 醱酵飼料 제조시 水分添加 水準에 따른 아미노산 組成에 관한 硏究 3. A Study on Amino acid Composition as added Moisture Levels

송영민,진상근,갈상완,이성대,김성찬,정맹화 진주산업대학교 농업기술연구소 2001 農業技術硏究所報 Vol.14 No.-

To estimate the essential and nonessential amino acid compositions as added moisture levels in manufacturing fermentation feedstuffs, the three kinds of experimental conditions, T1, T2, T3 were made with rice bran(90kg), leaf mold(10kg) according to added moisture 40kg, 60kg, and 80kg, respectively. In T1 and T2 cases, the contents of threonine, phenylalanine, histidine, aspartic acid, glutamic acid, cystine, arginine increased significantly during fermentation, however, in T3 case, the thereonine content increased significantly after 9 hours fermentation but decreased at 12 hours fermentation(P<0.05). In T1 and T3 cases, the contents of valine, leucine and arginine increased significantly after 9 hours fermentation and decreased at 12 hours fermentation(P<0.05). Methionine increased for three hours fermentation and then did not increase in content in the T1 and the T3. In T2 case, methionine level increased gradually for whole fermentation time. In T3 case, phenylalanine, histidine, aspartic acid, glutamic acid and cystine increased significantly after 9 hours fermentation, but decreased at 12 hours fermentation(P<0.05). In T1, T2 and T3, isoleucine, lysine, serine, proline, glycine, alanine and tyrosine increased significantly during fermentation time(P<0.05).

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

행동수정요법과 유산소운동이 중년기비만 여성의 혈청지질에 미치는 영향

남청웅,고영완,김종언 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was conducted to investigate the effect of the aerobics exercise and combined with behavior modification therapy on blood lipids level in obese middle-aged women. The 32 obese middle-aged women volunteers over 30% BMI were assigned to the aerobic exercise group(n=8), behavior modification therapy group(n=8), and aerobics exercise and combined with behavior modification therapy(n=8) or to a control group(n=8). To achieve presribed intervention for 16weeks, the experiment groups was bicycle ergometer and behavior modification therapy. Related materials were analyzed through one-way ANOVA, twoway ANOVA, and SNK-Test by using SPSS 10.0/PC+. The results of TC, TG, LDL-C, and % TC/HDL-C was significant decreased in experimental group, and the control group was no significant differences. Four groups were significant differences except to TG and the changes of HDL-C was significant increased in experimental group, and the control group was no significant differences. Four groups were significant differences. In conclusion, this study provides evidence that aerobic exercise, behavior modification, and aerobics exercise & combined with behavior modification is effective in changing blood lipids level in obese middle-agd women during 16 weeks.