Gonadal Maturation and Main Spawning Period of Haliotis gigantea (Gastropoda: Haliotidae)

So Ryung Shin,Hyeon Jin Kim,Dong Han Lee,Hyejin Kim,Young Chang Sohn,김재원,이정식 한국발생생물학회 2020 발생과 생식 Vol.24 No.2

This study was carried out to obtain information on the developmental biology and the management of biological resources of the abalone Haliotis gigantea in Korea. The sex ratio (female:male) in the present study was 1:1.7 and the proportion of females was 36.6% (n=106/290). Their gonadal structures displayed definitive seasonal changes which were similar in pattern to the changes in the gonad index (GI). The GI showed a pattern of definitive seasonal changes in both males and females it was high in the fall and low in the spring. The reproductive cycle could be categorized into the following six stages: inactive, early active, late active, ripe, spent, and degenerative stage. Based on the monthly changes in GI and stages of gonadal development, October to November was determined to be the main spawning period for H. gigantea on Jeju Island, Korea.

Differential expression of p63 isoforms in normal skin and hyperproliferative conditions

Kim, So-Young,Cho, Hyun-Joo,Kim, Dong-Seok,Choi, Hae-Ryung,Kwon, Sun-Bang,Na, Jung-Im,Jeon, Hye-Chan,Huh, Chang-Hoon,Youn, Sang-Woong,Cho, Kwang-Hyun,Park, Kyoung-Chan Blackwell Publishing Ltd 2009 Journal of cutaneous pathology Vol.36 No.8

<P>Background</P><P>The p63 is regarded as a potential stem cell marker.</P><P>Methods</P><P>Expression of p63 isoforms was examined in normal skin and hyperproliferative conditions including psoriasis and artificial skin equivalents (SEs). Rapidly adhering (RA) and slowly adhering (SA) cells were isolated, and Western blotting was performed.</P><P>Results</P><P>Expression of p63 (4A4) and p63 (H-137) is similar in all conditions, although there is some variation in psoriasis. However, expression of p63α (C-12) is markedly different. In normal skin, p63α (C-12)-positive cells were scattered in whole epidermis. But in psoriasis, p63α (C-12)-positive cells were observed at the tips of rete ridges. In SEs, p63α (C-12)-positive cells were not well observed. Western blot results showed that the RA cells express p63 (4A4) and p63 (H-137) strongly compared with SA or nonadhering (NA) cells. In contrast, SA or NA cells strongly express p63α (C-12).</P><P>Conclusions</P><P>Results suggest that both p63 (4A4) and p63 (H-137) can detect epidermal stem cells. But, p63 (H-137) seemed to be a better marker because p63 (H-137)-positive cells were more localized at basal layer. In addition, it can be said that p63α (C-12) can detect TAp63, which is important in differentiation of epidermis. Furthermore, it is concluded that molecular control of TAp63 is especially disorganized in hyperproliferative condition including psoriasis and SEs.</P>

Use of complementary and alternative medicine by lymphoma survivors in South Korea

Kim, Kisook,Kim, Soo Hyun,Ok, Oh Nam,Kim, Im-Ryung,Lee, Suyeon,Kim, So Hee,Kim, Won Seog,Ryu, Min-Hee,Lee, Moon Hee Elsevier 2018 EUROPEAN JOURNAL OF ONCOLOGY NURSING Vol.33 No.-

<P><B>Abstract</B></P> <P><B>Purpose</B></P> <P>We aimed to examine the experience of complementary and alternative medicine (CAM) use and its association with health-related quality of life (HRQOL) in lymphoma survivors in South Korea.</P> <P><B>Methods</B></P> <P>The participants were 869 lymphoma survivors from three hospitals in South Korea, all diagnosed with lymphoma at least 24 months prior to participation. Self-reported questionnaires were used to assess CAM use. The questionnaire addressed types of CAM used, sources of information about CAM, reason for CAM use, satisfaction with CAM use, discussion of CAM use with doctors, experience of side effects, costs of CAM use, and intentions to continue using CAM. HRQOL was measured with the EORTC QLQ-C30.</P> <P><B>Results</B></P> <P>Of the 869 participants, 42.2% had experience using CAM, and there were statistically significant differences among CAM users and non-users in terms of sex, religion, and time since diagnosis. A special diet (e.g., ginseng, chitosan, mixed cereals) was the most commonly used type of CAM, and most CAM users (82.1%) were satisfied with their CAM use. Most CAM users (77.5%) did not discuss the use of CAM with their doctors, and only 9.2% reported any side effects from CAM. CAM users showed significantly lower HRQOL scores than did non-users.</P> <P><B>Conclusion</B></P> <P>A significant number of lymphoma survivors in Korea have used CAM, and most CAM users are satisfied with their CAM use. Oncology nurses should be aware of the range of CAM use among patients and reflect their responses in their treatment and/or follow-up care.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A significant number of Korean lymphoma survivors (42.2%) have used CAM. </LI> <LI> A special diet was the most commonly used type of CAM. </LI> <LI> A majority of CAM users (77.5%) did not discuss the use of CAM with their doctors. </LI> </UL> </P>

Serum estradiol concentration in Korean postmenopausal women during hormone replacement therapy

( So Ra Kim ),( Sa Ra Lee ),( Yun Hee Koo ),( Ji Sun Kim ),( Sung Hoon Kim ),( Hee Dong Chae ),( Chung Hoon Kim ),( Ryung Moon Kang ) 대한산부인과학회 2007 대한산부인과학회 학술대회 Vol.93 No.-

Sex ratio, main spawning period and sexual group maturity of largehead hairtail Trichiurus lepturus

So Ryung Shin,Hyeon Jin Kim,Jung Jun Park,Jae Won Kim,Doo-Jin Hwang,Jung Sick Lee (사)한국해양생명과학회 2022 (사)한국해양생명과학회 학술대회 Vol.2022 No.10

Inhibitory Effect of Scopoletin Isolated from Sorbus commixta on TNF-α-Induced Inflammation in Human Vascular Endothelial EA.hy926 Cells through NF-κB Signaling Pathway Suppression

Hye Ryung Kang(강혜령),Hyo Jung Kim(김효정),Bomi Kim(김보미),Sun-Gun Kim(김선건),Jai-Hyun So(소재현),Soo Jeong Cho(조수정),Hyun Sook Kwon(권현숙) 한국생명과학회 2020 생명과학회지 Vol.30 No.4

마가목은 한방에서 기침, 천식, 기관지 질환 등의 치료에 이용되고 있다. 본 연구에서는 마가목(sorbus commixta) 수피의 chloroform 분획물로부터 3종의 triterpenoid 화합물과 1종의 coumarin 화합물을 분리하였다. 분리된 화합물의 구조는 MS와 1D-, 2D-NMR분석에 의해 확인하였으며, 이들은 lupeol, β-sitosterol, ursolic acid와 scopoletin으로 구조동정되었다. 분리된 화합물 중 scopoletin은 마가목에서 처음으로 분리된 화합물이다. Scopoletin은 식물에 널리 분포하고 있는 물질로써 항염증 활성을 가진 기능성 화합물이다. 분리된 화합물들의 혈관 염증 억제에 대한 효과를 평가하기 위해 in vitro에서 LDL 산화 억제능을 평가한 결과, 분리된 화합물 중 scopoletin (IC50=10.2 μM)이 강한 억제 활성을 나타내었다. TNF-α로 활성화된 인체혈관내피세포(EA.hy926)를 이용한 실험에서 scopoletin은 세포부착인자인 ICAM-1, VCAM-1, E-selectin의 발현을 저해하였고, THP-1 단핵구와 EA.hy926 혈관내피세포 간의 부착력도 약화시켰다. 뿐만 아니라, scopoletin은 TNF-α로 유도된 NF-κB 전사인자의 핵내 이동 및 IκBα의 인산화도 저해하였다. 따라서 마가목 추출물로부터 분리된 scopoletin은 NF-κB 신호전달의 억제를 통해 세포부착인자의 발현을 감소시키고, 단핵구의 혈관내피세포로의 부착을 억제시켜 혈관내 항염증 활성을 나타내었다. 이러한 실험결과, scopoletin은 혈관염증 반응으로부터 유도되는 죽상동맥경화증 치료를 위한 후보소재로서 이용될 가능성이 있다고 사료된다. Sorbus commixta Hedl. has traditionally been used as a remedy for cough, asthma, and other bronchial disorders. In this study, three major triterpenoids—lupeol, β-sitosterol, and ursolic acid and a coumarin, scopoletin, were isolated from a CHCl3-soluble fragment of the bark of S. commixta. Their structures were identified by spectroscopic analyses, including mass spectrometry (MS), 1D-, and 2D- nuclear magnetic resonance spectroscopy (NMR), as well as by comparing the data with data reported in the literature. Scopoletin was isolated from this plant for the first time. It is a nutraceutical compound contained in many plants that has been reported to exert diverse biological activities, including anti-inflammatory effects. This study examined the inhibitory effect of scopoletin on TNF-α-induced vascular endothelial inflammation. Unlike the marginal impact of other compounds against low-density lipoprotein (LDL) oxidation and vascular endothelial inflammation, scopoletin showed remarkable activity on LDL oxidation (IC50 = 10.2 μM) and exerted vascular anti-inflammatory effects in EA.hy926 human endothelial cells activated by TNF-α. It suppressed the expression of adhesion molecules, such as ICAM-1, VCAM-1, and E-selectin, and blocked the adhesion between THP-1 monocytes and EA. hy926 endothelial cells. It also inhibited TNF-α-induced NF-κB translocation from the cytosol to the nucleus. Moreover, IκBα phosphorylation, which was increased by TNF-α treatment, was reduced after treatment with scopoletin. Thus, scopoletin inhibited TNF-α-induced vascular inflammation in endothelial cells by suppressing the NF-κB signaling pathway. These results demonstrate that owing to its anti-inflammatory activity in the vascular endothelium, scopoletin has the potential to inhibit atherosclerosis development.

Proton Energy Straggling Measurement in Metal Foils by Using a Si(Li) Detector

Kye-Ryung Kim,Hong-Joo Kim,Jung-Ho So,Min Yong Lee,Yong-Sub Cho 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.3

Measurements of energy loss and transmitted energy straggling were performed using a Si(Li) detector for 28.6 MeV protons incident on thin foil targets of Al, Cu and Au in air. The straggling widths were studied as functions of the target thickness and the target species. The initial proton beam energy from the MC-50 cyclotron was 45 MeV and it was decreased to 28.6 MeV by passing it through a 2-mm-thick Al window, 34.5 cm of air, a 2-mm-thick Al degrader and 144.5 cm of air. This 28.6 MeV proton beam was incident on Al, Cu and Au foils of various thicknesses installed in front of a Si(Li) detector. As a result, the energy loss strongly depended on the target thickness and species and the transmitted energy straggling was not related to the target species. Compared with the calculated results based on a simple Bohr's expression, the measured values agreed with the calculated ones in the low energy loss region less than 60% of the incident proton beam energy. In the high-loss region, there was big mismatch of more than 25%. Measurements of energy loss and transmitted energy straggling were performed using a Si(Li) detector for 28.6 MeV protons incident on thin foil targets of Al, Cu and Au in air. The straggling widths were studied as functions of the target thickness and the target species. The initial proton beam energy from the MC-50 cyclotron was 45 MeV and it was decreased to 28.6 MeV by passing it through a 2-mm-thick Al window, 34.5 cm of air, a 2-mm-thick Al degrader and 144.5 cm of air. This 28.6 MeV proton beam was incident on Al, Cu and Au foils of various thicknesses installed in front of a Si(Li) detector. As a result, the energy loss strongly depended on the target thickness and species and the transmitted energy straggling was not related to the target species. Compared with the calculated results based on a simple Bohr's expression, the measured values agreed with the calculated ones in the low energy loss region less than 60% of the incident proton beam energy. In the high-loss region, there was big mismatch of more than 25%.

이동성 유전인자의 구조 및 생물학적 기능

김소원(So-Won Kim),김우령(Woo Ryung Kim),김희수(Heui-Soo Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.9

이동성 유전인자는 인간 유전체의 45%를 차지하며 기능성 유전자 내부로 자유롭게 들어갈 수 있다. 이들은 진화과정에서 중복현상으로 다수의 복사수로 생성되며, 생물종다양성 및 계통유전체학 분야에 기여한다. 이동성 유전인자의 대부분은 메틸화 또는 아세틸화 현상과 같은 후성유전학적 조절에 의해 제어된다. 다양한 생물종은 그들만의 고유의 이동성 유전인자를 가지고 있으며, 일반적으로 DNA트란스포존과 레트로트란스포존으로 나뉜다. 레트로트란스포존은 LTR의 유무에 따라 다시 HERV와 LINE으로 구분된다. 이동성 유전인자는 프로모터, 인핸서, 엑손화, 재배열 및 선택적 스플라이싱과 같은 다양한 생물학적 기능을 수행한다. 또한 이들은 유전체의 불안정성을 야기시켜 다양한 질병을 유발하기도 한다. 따라서, 암과 같은 질병을 진단하는 바이오 마커로 사용될 수 있다. 최근, 이동성 유전인자는 miRNA를 만들어 내는 것으로 밝혀졌으며, 이러한 miRNA는 타겟 유전자의 seed 영역에 결합함으로서 mRNA의 분해 및 번역을 억제하는 역할을 수행한다. 이동성 유전인자 유래의 miRNA는 기능성 유전자의 발현에 큰 영향을 미친다. 다양한 생물종과 조직에서 서로 다른 miRNA의 비교 분석 연구는 생물학적 기능과 관련하여 진화학과 계통학 영역에서 흥미 있는 연구 분야라 할 수 있겠다. Transposable elements (TEs) occupy approximately 45% of the human genome and can enter functional genes randomly. During evolutionary radiation, multiple copies of TEs are produced by duplication events. Those elements contribute to biodiversity and phylogenomics. Most of them are controlled by epigenetic regulation, such as methylation or acetylation. Every species contains their own specific mobile elements, and they are divided into DNA transposons and retrotransposons. Retrotransposons can be divided by the presence of a long terminal repeat (LTR). They show various biological functions, such as promoter, enhancer, exonization, rearrangement, and alternative splicing. Also, they are strongly implicated to genomic instability, causing various diseases. Therefore, they could be used as biomarkers for the diagnosis and prognosis of diseases such as cancers. Recently, it was found that TEs could produce miRNAs, which play roles in gene inhibition through mRNA cleavage or translational repression, binding seed regions of target genes. Studies of TE-derived miRNAs offer a potential for the expression of functional genes. Comparative analyses of different types of miRNAs in various species and tissues could be of interest in the fields of evolution and phylogeny. Those events allow us to understand the importance of TEs in relation to biological roles and various diseases.

Anti-angiogenic properties of bioconverted herbal formula

Hye Ryung Kang,Sun Gun Kim,Jae Hyun So,Young Mi Seok,Dong Hee Kim,Hyo Jung Kim 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Anti-senescence and anti-inflammation effect of Pandanus conoideus Lamk through nitric oxide and reactive oxygen species

( Hae Ryung Choi ),( Ye Jin Seon ),( So Ryeong Kim ),( Ji Suk Han ),( Hee Young Ahn ),( Ji Hye Kim ),( Yun Dan Kang ),( Jin Wan Park ),( Jong Soo Kim ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Objective: This study is to investigate if red fruit oil (RFO, Pandanus conoideus Lamk), which regulates NO and reactive oxygen species (ROS) metabolism, suppresses the senescence and inflammatory reaction of endothelial cells. Methods: The antioxidant activity of RFO was evaluated using 2,2,-diphenyl-1-picrylhydrazyl (DPPH) assay. To evaluate anti-senescence effect, 2 mM H2O2 was treated to human umbilical vein endothelial cells (HUVEC). 84 genes related to oxidative stress were evaluated using RT2-profiler PCR array. For anti-inflammatory effect, 1 μg/mL lipopolysaccharide (LPS) was treated to murine monocyte. The level of NO, pro-inflammatory cytokines, and signaling proteins were measured using ELISA, real-time PCR, and western blot analysis. Results: DPPH assay showed that antioxidant potent of RFO was 78.5 times 1mM ascorbic acid. RFO promoted the activation of superoxide dismutase (SOD) which induced degradation of endothelin (ET-1) in HUVECs. RFO increased the levels of SOD, glutathione peroxidases 3 (GPx), and peroxiredoxins (TPx), whereas it decreased the ROS metabolism gene and the oxidative stress responsive genes. In macrophages, RFO reduced nitric oxide (NO), prostaglandin E2 (PGE2), ROS and significantly both protein and mRNA level of iNOS. RFO inhibited the translocation of NF-κB p65 subunit, phosphorylation of I-κB, Extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) in a dose-dependent manner. Conclusion: RFO inhibited the senescence of endothelial cell by superoxide anion/ROS through regulating the ROS metabolism gene and the inflammatory cytokines through NF-κB. To conclude, RFO could be candidate of clinical target for treating vascular senescence or inflammation.