Collagenase와 esterase가 상아질 접착강도와 nanoleakage에 미치는 영향

정영정,한세현,김종철,이상훈,김정욱,김영재,장기택 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.3

본 연구는 상아질 접착계면에서 collagenase와 esterase가 접착강도와 극미세누출에 미치는 영향을 살펴보기 위해 시행하였다. 발치된 치아의 교합면 상아질에 Single Bond 2(SB)와 Clearfil SE bond(SE)를 사용하여 상아질-레진 접착시편을 제작하고,시편을 인산완충용액(PBS)에 24시간(I),또는 PBS(II),collagenase 용액(III),esterase 용액(IV)에 4주간 보관 한 후 질산은 용액으로 염색하였다. 시편의 미세인장접착강도(μTBS)와 질산은 침투 면적을 측정하여 다음과 같은 결과를 얻었다. 1. SB군의 접착강도가 II∼IV군에서 SE군에 비해 낮은 값을 나타내었다(p<O.05). SB군은 II∼IV군의 접착강도가 I군에 비해 낮은 값을 보였으나(p<O.05),SE군의 접착강도는 I∼IV군간에 차이를 보이지 않았다(p>O.05). 2. SB군의 질산은 침투 면적이 SE군에 비해 높았으며(p<O.05),SB군과 SE군에서 질산은 침투 면적은 I ∼ IV군간에 차이를 보이지 않았다(p>O.05). 3. 접착강도와 질산은 침투 면적은 SE군의 I, II,III군에서 음의 상관관계를 보였다(p<O.05). The purpose of this study was to evaluate the effects of collagenase and esterase on dentin bond strength and nanoleakage. Resin composites were bonded to occlusal dentin of premolars with Single Bond 2(SB) and Clearfil SE Bond(SE). After the microtensile specimens were prepared and stored in PBS for 24 hours(I) or, PBS(II), collagenase(III), esterase(IV) solution for 4 weeks, the specimens were stained with silver nitrate solution. Microtensile bond strength(μTBS) and silver penetration area were measured and, the results were as follows: 1. For group II, III, and IV, the bond strengths of SB were lower than those of SB(p<0.05). The bond strengths of SB II, III, and IV were lower than that of SB I(p<0.05). There was no difference among the bond strengths of SE I ∼ IV groups(p>0.05). 2. Silver penetration areas of SB were higher than those of SE for all storage groups(p<0.05). In SB and SE groups, there was no significant difference of silver penetration area among I ∼ IV groups(p>0.05). 3. SE I, II, and III showed inverse relationship between the bond strengths and the silver penetration areas(p<0.05).

상아질 접착에서 collagenase와 esterase가 미세인장결합강도에 미치는 영향

정영정,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2

상아질-레진 접착강도에 대한 collagenase와 esterase의 영향을 살펴보기 위해, 소구치의 교합면 상아질에 Single Bond 2와 Clearfil SE Bond로 접착을 시행하고 미세 시편을 제작하여 PBS, collagenase 용액, esterase 용액에 4주간 보관한 후 미세인장결합강도를 측정, 비교하여 다음과 같은 결론을 얻었다. 1. 모든 보관 용액에서 Single Bond 2의 미세인장결합강도는 Clearfil SE Bond보다 유의하게 낮았다(p<0.05). 2. Single Bond 2의 미세인장결합강도는 collagenase군이 PBS군, esterase군보다 낮았다(p>0.05). 3. Clearfil SE Bond의 미세인장결합강도는 esterase군이 PBS군에 비해 낮았으나(p>0.05), collagenase군보다는 높았다(p>0.05). Collagenase군은 PBS군에 비해 유의하게 낮은 미세인장결합강도를 보였다(p<0.05). The purpose of this study was to evaluate the effect of collagenase and esterase on the microtensile bond strength (μTBS) in dentin bonding. After resin composites were bonded to occlusal dentin, μTBS specimens were formed and stored in PBS, collagenase, or esterase solution. After 4-week storage, μTBS was determined and, the results were as follows: 1. μTBS values of Single Bond 2 were lower than those of Clearfil SE Bond for all storage medium (p<0.05). 2. In single Bond 2 group, collagenase solution lowered bond strength more than PBS and esterase solution (p>0.05). 3. In Clearfil SE Bond group, esterase solution lowered bond strength more than PBS(p>0.05). Collagenase solution lowered bond strength more than esterase solution(p>0.05) and PBS(p<0.05).

농산물중의 잔류농약 실태에 관한 연구(Ⅰ) : 고체상 추출에 의한 농산물중 잔류농약 분석에 관한 연구 Analysis of Residual Pesticides in Agricultural Products by Solid-Phase Extraction

허옥순,이재관,이정성,서정혁,주인선,허수정,김세은,김정근,신영희,유미자,김지연,심규창,김성환 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

신속 · 정밀하고 효율적인 짠류농약 검출을 위하여 극싱칼럼 (Extrelut-3')을 사옹한 SPE법을 시도하였다. 시료는 채소류체 사용하는 농약중 잔류성이 있고 식품공전상 시험 전처리가 각각 다른 Chlorothalonil등 6종의 농약을 선정하고 그들 표준액에 대한 PPIR수준의 농도로추출용라의 종류 및 용매량 등의 추출조건을 달리하여 회수율 등을 측정한 결과 최적의 분석조건을 얻었다. 1) 추출 용매량은 증가할수록 추출 수율이 높았고,용매 종류에 대한 추출수율은 각 농약별로 다소 차이는 있었으나 극성 정도가 비교적 높은 Ethyl acetate애서 평균 추출수율이 가장 높았다 2) 최대 평균회수율은 Ethyl acetate 60mL를 사웅할 경우로서 93.6%였다. 3)표준액을 시료에 첨가한 시험에서도 역시 Ethyl ace늘to가 푼출수율이 가장 높았으며 불순물 분리제거 효과도 LLE법 보다 월등히 우수하였다. 4) 각 농약별 검출한계는 치소 0.001ppin으로 농산물 중 미량 잔류하는 농약을 검출할 수 있을 것으로 사료된파. 그러므로 SPE법은 LLE법보다 경제적인 방법으로 판명되었으며, 앞으로 농산물 중 잔류농약 분석에서 LEE법을 대치할 쑤 있을 것으로 기대된다. Solid-phase extraction by polar column(Extrelut-3') was attempted to develope the fast and efficient method of detecting pesticide of farm product.5·ix kinds of pesticide used in farming fieldfrequently and different in pretreatment, were chosen from Korea Food Cord. Optfmal analysis condi-tions were determiBed from t31e recovery rate of standard pesticides according to extraction solvent andextraction volurae. Extraction yietd was increased as solvent polarity and extraction volume. Maximumrecovery rate was acquired at 93.6% when 60mL of ethyl acetate was used as eluent. Tllis method wasmore effective than liquid-liquid partition extract·ion method to eliminated the impurity and had 0.001ppm of detection limit. Therefore. solid-phase eEl=raction was expected to be economical substitute fortraditional liquid-liquid extT·action method.

Improved Internal Quantum Efficiency and Light-Extraction Efficiency of Organic Light-Emitting Diodes via Synergistic Doping with Au and Ag Nanoparticles

Cho, Changsoon,Kang, Hyunbum,Baek, Se-Woong,Kim, Taesu,Lee, Changyeon,Kim, Bumjoon J.,Lee, Jung-Yong American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.41

<P>This paper reports the distinct roles- of Au and Ag nanoparticles; (NPs) in organic light-Orating-diodes (OLEDS) depending on their sizes. Au and Ag-NPs that.are 40 arid 50 nm in size, respectively, are the most effective for enhancing the performance of green: OLEDs. The external quantum efficiencies (EQEs): of green OLEDs doped with An and Ag N.Pg, (40 and 50 rim, respectiVely) are improved by 29.5% and 36.1%, respectively, while the power. efficiencies (PE8) are enhanced by 47.9% and 37.5%, respectively. Furthermore, combining the Au and Ag NPs produces greater enhancements. The EQE and PE of the coci-oped OLEDs are improved by 63.9% and 68.8% respectively,-.through the synergistic behavior of the different NPs. Finite difference time-domain simulations confirm that the localized surfaee:plasmon resonance of the Au NPs near 580 nth improves the radiative recombination rate (k(rad)) of green-light emitters locally (<50 pm), while the Ag NPs cause relatively long, range and broadband enhancements in k(rad). The-Simulations of 'various domain sizes. Verify that the light-extraction efficiency (LEE),can, be enhanced by More than 4.2% by applying Ag NPS.. Thus, size-controlled Au and Ag NPs can synergistically enhance LEDS by improving both the internal quantum efficiency and LEE.</P>

톨루엔 노출 근로자에서 유전자적 요인, 생활습관 및 식이가 요중 마뇨산 배설에 미치는 영향

임현우,박정일,노영만,이강숙,이정민,이원철,이세훈,정치경 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.3

목 적 : 본 연구는 생물학적 지표 특성 연구를 위한 단면조사 연구로서 톨루엔 노출과 톨루엔의 생물학적 모니터링으로 이용되는 요중 마뇨산 농도간의 양-반응 관계에서 효과 변경인자로서 작용하는 유전자 ALDH2, CYPIAI, CYP2El의 다양성 분포를 조사하고, 이들 유전자가 요중 마뇨산 배설 농도에 미치는 영향을 알아보고자 하였다. 방 법 : 연구대상자는 경인지역에 위치한 22개의 사업체에서 페인트 제조 72명, 제조업에서 도장작업 50명, 인쇄업 12명, 접착작업 12명, 벽지의 코팅작업 14명등 160명의 근로자이었다. 성, 연령, 유기용제 작업장 근무경력, 흡연습관, 음주습관, 검사일 이전 밤에 음주 여부, 시료 채취전 6시간 이내에 섭취한 식품조사, 측정일 개인보호구의 착용 여부에 대하여 설문조사를 시행하였다. 톨루엔에 대한 개인노출량은 passive air sampler로 측정하였고, 요중 마뇨산은 요중 크레아틴으로 보정하여 측정하였다. 또한 aldehyde dehydrogenase 2 (ALDH2) , cytochrome P45O IAI(CYPIAI), 그리고 cytochrome P45O 2El(CYPfEl)의 유전적 다양성은 혈액으로부터 백혈구의 DNA를 추출하여 제한 효소 절단 길이 다양성 (restiction fragment length polymorphism, RFLP)법으로 검사하여 다음의 결과를 얻었다. 결 과 : 연구대상자의 요중 마뇨산 농도의 기하평균은 0.44g/g creatinine, 기하표준편차 2.80이었다. 톨루엔 노출 농도, 개인 보호구 착용여부, 안식향산이 함유된 식품의 섭취여부중 요중 마뇨산 배설농도에 영향을 미치는 인자는 톨루엔 노출 농도뿐이었다. ALDH2, CYPIAI, CYPfEl, 연령, 흡연, 근무기간에서 회귀계수간에 차이를 보이는 경향이 있었다. 요중 마뇨산 농도에 대한 다중회귀분석에서 톨루엔, ALDH2, CYPIAI, CYP2El유전자형의 회귀계수가 통계적으로 유의하였다. 결 론 : 톨루엔 노출로 인한 요중 마뇨산 배설 농도는 ALDH2, CYPIAI, CYP2El유전자 다양성에 의해 영향을 받기 때문에, 유전자형의 대사능의 차이를 고려하여 마뇨산 기준 농도를 찾는 연구가 이루어져야 하겠다. Objectives The purpose was to investigate the distributions and the effects of genetic polymorphism of aldehyde dehydrogenase 2(ALDH2), cytochrome P45O IAI (CYPIAI), and cytochrome P45O 2El (CYPfEl) on the toluene metabolism. Methods : The subacts consisted of 160 workers who were exposed to toluene in different industries such as paint manufacturing, painting on steel and wood products, printing, bonding, and coating. The exposed toluene level was monitored by passive air sam paler, and the questionnaire variables were age, sex, smoking, drinking, previous night's drinking, use of personal protectlve equipment, work duration, and taking benzoic acid containing food. The urinary hippurric acid collected in the end of shift was corrected by urinary creatinine concentration. The genotypes of ALDH2, CYPIAI, and CYP2El were investigated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods with DNA extracted from venous blood. Results'The geometric mean and the geometric standard deviation of urinary hippuric acid concentration were 0.44 g/g creatinine and 2.80. The urinary hippuric acid concentration was significantly related to personal exposed toluene level among personal exposed toluene level, use of personal protective equipment, and benzoic acid containing food diet. The slope differences of the regression for ALDH2, CYPIAI, and CYP2E1 genetic polymorphism, age, smoking, and work duration tended to be significant. In multiple regression analysis, the regression coefficient of toluene, ALDH2, 7YPIAl, CYP2E1 genetic polymorphism were significant. Conclusions : From the above results, urinary hippuric acid level after toluene exposure was significantly affected by the genetic polymorphism of ALDH2, CYPIAI, CYPfEl. It is needed further investigation of the urinary hippuric acid level considering the effect of genetic polymorphism.

사상의학과 형상의학의 유형분류의 연관성에 관한 연구

장아름,전수형,박세정,이현미,김규곤,이용태,지규용,이인선,김종원,Jang, A-Reum,Jeon, Soo-Hyung,Park, Se-Jung,Lee, Hyun-Mi,Kim, Kyu-Kon,Lee, Yong-Tae,Ji, Gyu-Yong,Lee, In-Sun,Kim, Jong-Won 사상체질의학회 2010 사상체질의학회지 Vol.22 No.3

1. Objectives: This study was performed to research the relationship between Sasang Constitutional medicine and Hyungsang medicine. 2. Methods: Four hundred and thirty applicants are involved in this study. They are devided into Sasang Constitution and Hyungsang Classification 3. Results and Conclusions: There are significances between Sasang Constitutional diagnosis and Hyungsang Classification. There are significantly more Bangkwang-body, Gi-kwa, Taeyang-hyung, Yangmyung-hyung in Taeyangin and Bangkwang-body, Jung-kwa, Soyang-hyung, Yangmyung-hyung in Soyangin. There are significantly more Dam-body, Gi-kwa, Taeyang-hyung, Yangmyung-hyung in Taeeumin and Bangkwang-body, Gi-kwa, Taeyang-hyung, Yangmyung-hyung in Soeumin.

양식 넙치(Paralichthys olivaceus)에서 발생한 랩도바이러스성 질병의 병리조직학적 특징

이남실,강형길,최희정,전세규,박남규,허민도 한국어병학회 2002 한국어병학회지 Vol.15 No.1

2000년 4월경부터 (수온7-13℃) 우리나라 동해안과 남해안 육상 수조에서 사육되던 넙치에서 체색흑화, 복수저류, 비장종대의 육안적 소견을 보이는 질병이 발생하여 대량폐사를 불러왔다. 병리조직학적 소견으로, 비장과 신장의 조혈조직에서 괴사병변이 주로 나타났다. 비장과 신장의 간질조직에서 괴사소가 관찰되었으며, 임파구, 괴사세포의 침윤이 나타났다. 비장 조직의 전자현미경적 관찰에서 변성혈구 내 세포질에서 탄환형의 바이러스 입자가 관찰되었는데 직경과 길이가 각각 약 50nm, 170nm였다. 이상의 결과로 본 질병은 rhabdovirus에 의한 질병으로 판단되었다. On April of 2000 and February of 2001, a disease characterized by darkening, ascites and enlargement of the spleen occurred in flounder, Paralichthys olivaceus, farmed near northern and eastern coasts of Korea. Various degree of necrosis including liquefactive-type was observed mainly in hemopoietic tissue of spleen and kindney. Certain tissue such as branchial soft tissue including epithelium showed a similar necrotic findings. But some tissues such as intentine had several necrotic clusters of wandering cells, being possibly leukocytes. Necrotic evidences of blood cells were evident in various tissues including hemopoietic tissues. Necrobiotic bodies were frequently encountered in blood smear preparations. Bullet type-virious were detected in the spleenic cells. Each virion was about 500 nm in diameter and 170nm in length. From these results, this disease was thought to be a rhabdoviral disease.

구강병 예방진료 일부항목의 건강보험급여를 위한 재정추계 연구

이영수,정세환,이규식 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.4

Objectives: This study aims (1) to determine the actuarial budget of national insurance payment for some items of preventive dental service and (2) to obtain basic data to develop dental policy which is for including preventive dental service within insurance payment. Methods: Pit and Fissure Sealing (PFS), Self-plaque Control Instruction (S-PCI), and Fluoride Topical Application (FTA) were selected for some items of preventive dental service. The actuarial budget of insurance payment was calculated through multiplying followings; 1) Applicable population or number of teeth (based on population data and Korean National Oral Health Survey 2000) 2) The usual and customary fee of 2001 from three dental colleges which have preventive dental clinic or RBRVS (Resource-based relative value scale) fee which was multiplied the RBRVS developed through the research of Yonsei University and conversion factor Korean Ministry of Health and Welfare had announced at 2001 as 55.4 won 3) 70% of insurer share rate 4) The utilization rate of the items of preventive dental service presumed to be at least 5% and at the most 36% Results: Actuarial budgets of 1) PFS are 18.8 billion (usual and customary fee) and 5.7 billion (RBRVS fee) for 5% utilization rate and 135.1 billion (usual and customary fee) and 41 billion (RBRVS fee) for 36% utilization rate when limited to first molar and second molar which are firstly facing teeth of 6-14 age group; 2) FTA are 2.5 billion (usual and customary fee) and 1.5 billion (RBRVS fee) for 5% utilization rate and 18.3 billion (usual and customary fee) and 10.6 billion (RBRVS fee) for 36% utilization rate when applied only to 6-14 age group annually once; 3) S-PCI are 9.1 billion usual and customary fee) and 5.5 billion (RBRVS fee) for 5% utilization rate and 65.7 billion (usual and customary fee) and 39.8 billion (RBRVS fee) for 36% utilization rate when limited over 12 age group who could have periodontal pocket. Conclusions: The actuarial budgets of PFS, FTA, and S-PCI if they were included within insurance payment system are minimum 13 billion and maximum 219 billion. It is presumed, however, that over 1,000 billion for dental treatment and the pain and time consuming from oral &ease could be decreased when dental caries and periodontal disease were prevented effectively. As a conclusion, preventive dental service should be included to the insurance payment system to decrease budget.

이동로봇의 동적 경로계획

이우송,오세봉,안용근,문정훈,한성현 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

This paper propose a new path planning method for mobile robots. The creation method of the course approaches with the method of the frame line graph base where the calculation time becomes disturbance. The graph composition where is not the static course plan method of existing, a course search and repeats continuously changes a graph and proposes the course the dynamic routing plan method. The method which is proposed is composed of a course creation phase and a course improvement phase, each phase includes a graph composition and a course search process.

서울대학교치과병원 장애인진료실에서의 치과치료 분석

이현정,김영재,김정욱,장기택,이상훈,김종철,한세현 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.2

전신마취하에서의 치과치료는 자발적인 협조가 불가능하거나 전신적인 문제가 있는 경우, 다수의 치료를 한 번에 하고자하는 경우 등에 행해진다. 이 연구는 서울대학교치과병원 장애인진료실에서 외래 전신마취하에 행해진 환자와 치과치료를 분석함으로써 보다 나은 장애인 진료 시스템을 위한 방안 제시에 도움이 되고자 하였다. 2002년 6월 11일부터 2006년 12월 31일까지 장애인진료실에서 치료 받은 432명의 환자들을 대상으로 조사한 결과 15세 이하의 환자가 50.46%로, 소아 및 청소년 환자가 높은 비율을 차지하고 있었다. 진료과는 보존과, 소아치과, 구강외과, 치주과, 보철과 순으로 나타났으며 치료내용을 보면 보존치료와 발치가 주를 이루고 있었다. 또 2회 이상 전신마취를 시행한 환자가 14.1%나 되었다. 본 연구는 장애인 환자를 위한 보다 포괄적인 치과 치료 및 진료과들 간의 긴밀한 협진 체계를 구축하고 필요한 인력과 설비를 보충함으로써 장애인 환자들에게 보다 보존적인 치과치료와 편안한 치료 환경을 제공해야 할 필요성을 제시하고 있다. Seoul National University Dental Hospital opened The Clinic for The Disabled to provide dental treatment under outpatient general anesthesia. 432 patients underwent general anesthesia for dental treatment. The informations such as the pattern of dental treatment, the reasons of general anesthesia, the characteristics of the patients were analyzed. Followings are concluded. 1. The patients below 15 years old made up 50.60%. 2. The reasons for general anesthesia included mental and physical disabilities, systemic disease, dental phobia, and so on. 3. Restorative treatment was the most common procedure with the average of 9.7 teeth treated per one patient. 4. 62(14%) patients underwent general anesthesia for dental treatment more than once. 5. The efforts for better multidisciplinary system to provide more comprehensive and effective dental treatment including periodic recall check, preventive treatment for handicapped patients are necessary.