소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

섬유상 담체 종류에 따른 생물학적 오수처리 비교연구

이수구,임병란,한종옥 서울産業大學校 1994 논문집 Vol.40 No.1

This study was performed to investigate the treatability of sewage from department store by attached growth biological treatment method using several fibrous media such as Supermodule made of polyvinylidine chloride and acry fiber(FBC), ringlace made of polyvinylidine chloride(HBC) and polypropylene(PP). The pilot plant was set up in the field of K department store and four consecutive aerated reactors were packed with each media and maintained in the condition of hydraulic retention time 12 hours. Experimental results showed that the effluent BOD concentration was stable even though the influent concentration was largely fluctuated in the range of 300~900㎎/L. BOD removal efficiencies in HBC, PP and FBC systems were 90%, 87% and 94%, respectively. The influent BOD was mainly removed in the first, second and third reactors and the removal rate in the fourth reactor was the lowest due to the low concentration and low influent load. The concentration of biomass attached to fibrous media was the highest in the FBC system compared with HBC and PP systems. Total concentration of volatile solids in the FBC system was 5,900 ㎎/L which was high compared with the other media systems. The production ratios of waste sludge were 9.2, 8.45 and 7.3 gDS/㎥ of influent sewage in HBC, PP and FBC systems, respectively.

한약재가 Aspergillus parasiticus R-716의 生育과 aflatoxin 生成에 미치는 影響

구성회,이용욱,정덕화,김종규 한국식품위생학회 1988 식품위생학회지 Vol.3 No.2

한의원에서 오래 前부터 사용되어 오고 있는 몇 종의 한약재를 구입하여 이들을 chloroform으로 추출하여 추출물을 조제한 다음 공시균(Aspergillus parasiticus R-716)의 生育과 aflatoxin 生成에 미치는 影響을 조사한 결과 다음과 같은 結論을 얻었다. 1. 목단 추출물 첨가군에서 가장 현저하게 공시균의 生育이 저해되었고 그외의 첨가군에서도 강황, 봉출, 향부자, 백작액, 백출의 順으로 저해되었다. 2. Aflatoxin 生成은 백출과 강황 추출물 첨가군에서만 저해되었고, 나머지 첨가군에서는 오히려 증가되거나 효과가 적었다. 3. 백출 추출물 0.2ml첨가시 3일째부터 菌體 生成을 시작하여 9일째에 0.953g/30ml이었고, aflatoxin 함량은 792㎍으로서 대조군에 비해 약 50%가 감소되었다. 菌體 g當 aflatoxin 함량은 992㎍으로서 대조군의 1,467㎍에 비해 역시 크게 감소되었으며 NADPH oxidase 活性은 대조군보다 높아 백출 추출물이 공시균의 生育과 aflatoxin 生成에 저해 효과가 있는 것으로 나타났다. The possible effects of some oriental herbs, which have been used to treat cancerlike disease in Korea, on growth and aflatoxin production by Aspergillus parasiticus R-716 were investigated. Zedoaria rhizoma, Curcuma longa, Cyperus rotundus, Angelica gigas, Paeonia albiflora, Paeonia mountan, Atractylis ovata, and Pulsatilla koreana were extracted with chloroform. Among them the extract of Paeonia mountan was remarkably effective on the growth inhibition, and Curcuma longa, Zedoaria rhizoma, Cyperus rotundus, Paeonia albiflora, Atractylis ovata also inhibited the growth. The extract of Atractylis ovata and Curcuma longa, also inhibited the aflatoxin production but the others showed no effect at all or sometimes stimulated effect. With the addition of 0.2ml extract of Atractylis ovata in 30ml SLS medium, the growth was delayed for about 2 days, and after 9 days, mycelium weight was 0.953g, and total aflatoxin was reduced 50%(792㎍)of that produced in the control(1547㎍). Aflatoxin per mycelium weight was decreased 32%(992㎍)of that produced in the control(1467㎍), but NADPH oxidase was higher as compared to the control. The extract of Atractylis ovata appeared to have a inhibitory effect on the growth and aflatoxin production of Aspergillus parasiticus R-716.

다단계 검출알고리즘을 이용한 웨이블렛기반 이미지 워터마킹

이구영,정용덕,이종원,김정화 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.2

In this paper, we propose a watermarking algorithm of multiresolution watermarking based on wavelet transform for digital images. Watermark insertion is add to pseudo random sequences to the wavelet coefficients at the high and middle frequency band of the discrete wavelet transform. Instead of previous method, proposed multiresolution watermark decoder that allows efficient identification of the embedded a bank of watermarking key without prior notice of it. Proposed detection method aims at ensuring the maximum exactitude in the detection of the owner identification key and, minimizing the number of false positive detection.

가공속도와 슬러리의 가공온도에 따른 웨이퍼 폴리싱의 표면특성에 관한 연구

이정택,원종구,신태희,이은상 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

Silicon wafers which are the starting materials for the industry of semiconductor must be very flat in order to print circuits on them by lithographic processes. Therefore, the wafer polishing is an essential process used in the fabrication of silicon wafers to achieve a globally planar and mirror-like wafer surface. The surface roughness in the wafer depends on the surface properties of the carrier head unit along with other machining conditions such as polishing pad, Processing velocity, machining temperature, down-force distribution and so on. Also the applied down-force is an important parameter of polishing process. This study shows the surface characteristics between the polishing pad and the wafer carrier unit by wafer polishing system head. The experiments were performed to observe the down-force and temperature when the wafer carrier head unit was pressed down onto the polishing pad. To measure the down-force distribution in processing velocity, the experimental set up used the loadcell to get the signal of the applied pressure against the polishing pad in actual processing with detecting temperature in slurry. Thus the optimum condition selection of ultra precision wafer polishing is using loadcell and infrared temperature sensor. The optimum condition is selected to use a result data that measure a pressure in processing velocity. By using optimum condition, it helps to achieve an ultra precision mirror like surface.

성인 급성 신부전의 원인 및 예후인자 분석

구영선,장윤경,양종오,강민규,황평주,김종학,나기량,이강욱,신영태 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Acute renal failure is a frequent complication in hospitalized patients and is strongly related to the mortality. The Clinical outcome and prognostic factors of acute renal failure(ARF) have been analyzed by many authors. The present study describes the etiologic and clinical aspects, as well as other factors related to mortality. All the patients suffering from acute renal failure admitted during the period of January 1993 - August 1998 were included in the study. The average age of the patients was 52±17 years and mortality rate was 24%. The causes of acute renal failure were hemorrhagic fever with renal syndrome(HFRS), sepsis, renal hypoperfusion, urinary tract obstruction, acute tubular necrosis, etc. The etiology of ARF was a significant prognostic factor on mortality in ARF. Other significant prognostic factors were oliguria, organ failure, use of vasoconstrictors, hypotension, serum bicarbonate, premorbid conditions, sepsis, neurologic complications, gastrointestinal bleeding. On the other hands, operation, sex, anemia, thrombocytopenia, hypoalbuminemia, BUN, serum creatinine, and hyperkalemia were not significant factors for the mortality. We conclude that major prognostic factors of acute renal failure arc premorbid conditions, sepsis and multiorgan failure, and they are responsible for persistent high mortality of acute renal failure despite of advances of medical care.

웨이퍼 폴리싱의 가공 조건에 따른 표면 특성에 관한 연구

이정택,원종구,이은상 한국공작기계학회 2007 한국공작기계학회 추계학술대회논문집 Vol.2007 No.-

The wafer polishing is an essential process used in the fabrication of silicon wafers to achieve a globally planar and mirror-like wafer surface. The surface roughness in the wafer depends on the surface properties of the carrier head unit along with other machining conditions such as polishing pad, working speed, temperature, down-force and ETC. Also the applied down-force is an important parameter of polishing process. This study shows the surface characteristics between the polishing pad and the wafer carrier unit by wafer polishing system head. The experiments were performed to observe the down-force and temperature when the wafer carrier head unit was pressed down onto the polishing pad. To measure the down-force distribution, the experimental set up used the loadcell to get the signal of the applied pressure against the polishing pad in actual processing with detecting temperature using IR temperature sensor.

강의 현대화 추진 및 활용방안 연구

구경완,이근복,장종국,김재홍,김영환,최제영,이승훈 永同大學校 1997 硏究論叢 Vol.3 No.1

본 연구는 각종 첨단 기자재를 활용한 강의 현대화 방안을 모색함으로서 공학교육의 효율성을 도모하고 학생들의 학습동기를 유발하여 창조적 능력을 갖츤 전문기술 인력을 양성하는 데 도움을 주고자 하는 것이다. 이를 위하여 현행 공학교육의 현황과 문제점을 분석하고 강의 현대화 사례를 조사하여 본 연구의 기초자료로 삼고자 하였으며, 학부·전공별 강의 현대화 추진 실태분석 및 개선방안을 모색한 후 이상의 연구결과를 종합하여 향후의 강의 형대화 추진방향을 걸정하였다. This study aims to help bring up the well-skilled and creative engineers by searching the modernization of education using various high technology facilities. For this, we analyzed the general condition and problems of current engineering education and surveyed the case study of the modernization of education as basic study. After analyzing the existing situation of promotion searching the proper improvement methods of it, we proposed the new direction of the modernization of education from now on.

전산화 단층촬영술을 이용한 악하선 타석증의 진단

구치균,이종갑,이제호,최형준 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.3

Sialolithiasis is the formation of calcareous concretions within ductal system of a major or minor salivary gland. They are formed by deposition of calcium salts around a central nidus which may consist of desquamated epithelial cells, bacteria, foreign bodies, or products of bacterial decomposition. An ll-year-old boy complained of pain during meals and intermittent mild swelling in the right submandibular region. Although it was not detected in true occlusal radiograph. panoramic radiograph showed a round radiopaque mass 3mm in diameter. Computed tomography (CT) was taken for locating the stone and 3-dimensional reconstruction was per-formed . Under general anesthesia, sialoadenectomy was done through extraoral approach. diagnosis of submandibular sialolithiasis using high-resolution CT with reconstructions was helpful for surgical decisions, namely radical removal of the submandibular gland and its duct.

BGA 솔더 볼 로딩장치의 최적설계

이준환,이종원,구홍모 중앙대학교 생산공학연구소 1999 생산공학연구소 논문집 Vol.8 No.2

최근의 정보화 장비와 멀티미디어의 소형화 추세는 생산 설비중 반도체 패키지 기술레 힘입은 바 크다. BGA(Ball Grid Array types of package)와 CPS(Chip Scale Packages)는 QFP(Quad Flat Packages)를 대체하는 기술로 인정되고 있다. 볼을 탑재하는 장치는 기존에 진공 - 흡입장치가 많이 사용되어 왔다. 시소 타입 로더에서는 플래이트의 각도와 카트리지의 속도가 중요한 요소로 간주된다. 본 연구에서는 이에 대한 최적 속도와 각도를 제안하였다. With the trends of the recent micro-miniaturization of mutimedia and information equipment, electronic components packaging technology is playing a key role in production technology. BGA(Ball Grid Array thpes of package) and CSP(Chip Scale Packages) are replacing QFT(Quad Flat Packages). Vacuum-suction type loader is a popular loading device. For the seesaw type loader, the velocity fo cartridge and the angle of plate are very important elements. In this study, the optimal values of the velocity and the angle are proposed for the seesaw type loader.