곤충의 수컷생식부속선물질 : Aedes aegypti 모기를 중심으로

이종진,오세원 고려대학교 한국곤충연구소 1995 昆蟲硏究誌 Vol.21 No.1

곤충의 수컷생식부속선물질: Aedes aegypti 모기를 중심으로 이종진, 오세원^*(전북대학교 농생물학과, 광주보건대학 치위생과^*) Aedes aegypti 모기의 수컷생식부속선물질이 암컷의 난성숙, 산란전행동,산란행동 및 숙주탐색행동에 미치는 생리학적 기능을 고찰하였다. 흡혈과 함께 교미한 암컷은 미교미암컷 보다 훨씬 더 많은 난발육이 진행되었으며, 교미와 관련하여 정상교미, 수컷생식부속선전입 혹은 수컷생식부속선 균질물 주입 등의 경우 이들 모두는 난성숙 암컷의 산란전행동을 유도한다. 수컷생식부속선물질의 주요 구성성분은 종특이성을 가지고 있으며, 수컷생식부속선물질의 생물학적 활성은 4˚C에서 24시간, -10˚C에서 2주간 지속되나 그 이상의 시간이나 가열 등에 의해 불활성화된다. 교미시 전달되였거나 암컷에 직접 주입된 수컷생식부속선물질은 난성숙이 이루어진 암컷의 숙주탐색 행동을 현저히 감소시킨다. 따라서 교미시 암컷으로 전달된 Aedes aegypti 수컷생식부속선물질은 암컷의 난성숙, 산란전행동, 산란행동을 유도하는 반면 숙주탐색행동을 억제하는 등 성충암컷의 여러가지 생리 및 행동변화에 직접적인 영향을 미친다. An endogenous physiological mechanisms of the male accessory gland substances (MAGs) of Aedes aegypti mosquitoes are reviewed in regard to the effects on female behaviors such as egg maturation, pre-oviposition, oviposition, and especially host-seeking. Mated females that were maintained on water for 4 days before ingesting blood were more likely to develop eggs than were unmated females. Normal mating, the implantation of whole glands, or the injection of male accessory gland homogenates, all induced pre-oviposition behavior in gravid virgin females. The components of MAGs are species-specific. MAGs retained their biological activity when stored at 4˚C for 24h or when maintained at -10˚C for 2 weeks, but lost their activity after longer periods and were inactivated by heating. MAGs, when transferred during mating or injected directly into females, significantly reduced the host-seeking behavior of the female when they gravid.

패킷 데이터를 이용한 네트워크 기반의 침입 탐지 시스템에 대한 연구

이종진,임현근 長安大學 2005 長安論叢 Vol.25 No.1

고속 인터넷에 연결된 가정용 PC도 해커들의 주요 공격목표가 되고 있는 요즘에는 기업 및 개인까지 영향이 미치는 등 시스템에 많은 주의를 요하며, 개인에서 기업까지의 침투는 사실상 100%차단이 거의 불가능하다. 최근의 인터넷 공격은 기존의 공격과 달리 대규모적이고 인터넷 공간에서의 혼란을 야기 시키고 있으며 침입자들은 단순히 자신의 해킹실력을 과시하고자하는 호기심뿐만 아니라 국가와 기업의 정보기반에 혼란을 주고 있다. 이에 본 논문에서는 네트워크 기반의 침입 탐지 시스템을 제안함으로서 네트워크상에 존재하는 모든 호스트들에게 시스템 변화를 주지 않고 외부로부터의 침입을 탐지 하려고 한다.

천연곡물세안제 구성성분의 항산화력 비교

이종진,이미향,김성호 순천향대학교 기초과학연구소 2008 순천향자연과학연구 논문집 Vol.14 No.2

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소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

씨고자리파리의 에스테라제에 대한 Dichlorvos의 저해효과

이종진,최만영 전북대학교 유전공학연구소 1989 遺傳工學硏究所報 Vol.2 No.1

씨고자리파리의 변태에 따른 단백질 및 에스테라제의 변화, 에스테라제의 활성에 대한 dichlorvos의 영향, 그리고 에스테라제의 α-Naphthylacetate 가수분해 활성에 대한 dichlorvos의 저해효과를 전기영동법과 Gomori의 효소활성 측정법에 의해 조사한 결과 총 28개의 단백질과 14개의 에스테라제 밴드를 분리하였으며, dichlorvos의 중앙치사약량은 암컷성충에서 15.76μg/g, 3령 중기 유충에서 52.17㎍/g이었다. Dichlorvos 처리후 암컷 성충의 에스테라제 밴드의 변화가 관찰되었다. 雌成蟲 homogenate의 전체 esterase 활성중 약 40.9%가 dichlorvos농도 약 6×10^-5M에 의해서 沮害를 받은 것으로 나타났다. Changes in protein and nonspecific soluble esterase patterns, the effect of dichlorvos on esterase activity, and the inhibition of α-Naphthylacetate hydrolyzing activity of esterase by various concentrations of the dichlorvos in the seedcorn maggot, Delia platura(Meigen), were investigated using PAGE and Gomori's colorimetric methods. Through all stages, 28 electrophoretic protein bands and 14 esterase isozyme bands were separated. LD 50 values of the dichlorvos on the larvae and female adults were 52.17μg/g and 15.76μg/g, respectively. Difference of electrophoretic esterase isozyme band patterns between knock-downed, survived and control adults after treatment of dichlorvos were observed. 40.9% of esterase activity to a-Naphthylacetate in homogenate of female adults was inhibitable by 6×10^-5M dichlorvos.

수술 및 Praziquantel을 이용한 뇌 낭미충의 치료 효과

이종진,박광섭,이동열 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.4

이상의 뇌낭미충증 8례에 대한 임상고찰 및 치료의 결과는 다음과 같다. 1) 연령분포는 32세에서 56세로 평균 43.1세였고 남자가 6례 여자는 2례였다. 2) 병변의 위치에 따른 분류는 뇌실질형(6례), racemose형(1례), 혼합형(1례)였다. 3) 임상증상은 전간 및 두통이 주소이며 시력장애, 운동장애와 의식변화등을 나타냈다. 4) 뇌전산화단층촬영상 낭성 저밀도 음영과 환형의 대조강화가 나타나는 경우가 많고, 치료후 7례에서 추적뇌전산화단층촬영상 병변의 현저한 감소 및 소실이 보이고 1례에서만 1년 6개월 후 재발된 병변을 나타냈다. 5) 낭미충에 대한 면역효소측정 검사를 시행한 6례에서 혈청과 뇌척수액에서 모두 양성이었고, 치료 3개월 후 추적면역효소측정 검사상 역가의 증가를 보이나 6개월 후 추적검사를 시행한 2례 중 1례는 감소, 1례는 증가를 나타냈다. 6) 수술은 낭종이 주위 장기에 종괴효과를 나타낸 2례에서 시행하고 수술후 praziquantel투여를 하였다. 7) Praziquantel 투여는 50㎎/㎏/day로 2주간 3회 분복하는 것이 이상적이나 쇄약하거나 부작용이 발현시 감량하여 30㎎/㎏/day로 3주간 투여 할 수도 있다. 8) Praziquantel 투여시 부작용은 두통, 현기증, 오심 및 장관장애와 전간, 국소신경학적이상등이고 부작용을 줄이기 위해서 치료이전부터 corticosteroid 투여가 바람직하다고 생각된다. 9) Praziquantel 단독으로 치료한 예와 수술 및 Praziquantel을 병행하여 치료한 예에서 결과는 혼합형을 제외하고 우수한 효과를 나타낸다. 향후 피낭미충증 치료는 침범장소 및 형태에 따르는 치료방법과 praziquantel 투여시기, 투여방법, 투여량, 재발시 투여에 대한 연구가 더 많이 연구 관찰되어져야 할 것으로 사료된다. The eight patients of neurocysticercosis have been diagnosed from 1985 to 1987 in the department of neurosurgery at The Baptist Hospital. Six patients had parenchymal cysts, one had a racemose cyst and the other one had a mixed cyst. The blood serum and CSF ELISA on six patients had a positive reaction for cysticercosis but the follow-up ELISA titers were variable three months later on these four. The racemose and mixed cysts were removed surgically due to mass effect and followed by praziquantel therapy. The parenchymal cysts were treated with praziquantel only. Praziquantel was given daily at 50㎎/㎏ of body weight for two weeks or 30㎎/㎏ of body weight for three weeks. The common side effects of praziquantel were headache, dizziness, nausea, seizure and focal neurological deficits. These side effects could be prevented or minimized by corticosteroid. The result of praziquantel therapy was excellent in all the patients except the mixed cyst.

중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구

이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.

劉熙載의 論詞 小攷 : 蘇·辛에 관한 詞品을 中心으로

李鍾振 韓國中國語文學會 1980 中國文學 Vol.7 No.-

周濟 詞論中의 寄託論

李鍾振 이화여자대학교 한국문화연구원 1986 韓國文化硏究院 論叢 Vol.48 No.-

Chou Chi(周濟, 1781~1839), a famous theorist on tz'u(詞), heightened the literary standing of tz'u of Ch'ang-Chou School(常州詞派). His views on tz'u can be seen in "The Prefact to Tz'u-pien(詞辨序)", "Chieh-ts'un-chai lun tz'u tsa-chu(介存齋論詞雜著)", "Sung ssu-chia tz'u hsu-lun(目錄序論)", where the method of studying tzu'(詞學法), the theory of appreciation(鑑賞論), the theory of writing(創作論), the theory of criticism(批評論), etc. are mentioned. Among them, the theory of allegory is not only Chou Chi's Key point on tz'u theory, but acts as an insistent main idea in his opinions on tz'u. His theory of allegory is derived from the theory of Pi-hsing(比興說) which Chang Huiyen(張惠言, 1761~1802), the founder of Ch'ang-chou School, suggested to heighten the standing of tz'u theory. Pi and hsing are two elements of six tropes(六義) of Shih-ching, which mean simile and metaphor respectively. Chang Hui-yen's theory of Pi-hsing could be summarized as "external sound corresponding to an internal though(意內言外)". What it means is that though the words in tz'u are easy and shaollow, their implied meanings are very profound. This view, derived from Pragmatic theories on poetry of Han(漢) Confucians who pursued "Subline words with deep meaning(微言大義)", is suggested to explain that love songs also reflect the six tropes of Shih-ching. But the idea of "external sound corresponding to an internal though" bought about a bad custom to fail to appreciate or distort the original meaning of tz'u. Chou Chi, therefore, suggests the use of allegory by which writers express metaphorically their own virtues and patriotism by singing things of same sorts. But it is very difficult to heighten the literary standing of tz'u only by allegory. So he drew "the vagueness of allegory"(無寄託) out of allegory to be able to explain the emotions differently according to the learning, the status of living and the moral culture of readers. The vagueness of allegory, an action of moving emotions(移情作用) which can be obtained by objectifying the subjective emotions into the objective things that reflect the associations of idea and allegories, is possible only when the allegory is profound. So the theory of allegory, pursuing deeper taste and simpler and honester stage, could be set forth by the vagueness of allegory. Chou Chi also insists that apprentice writer of tz'u should deliberately work to have an allegorical dimenion(有寄託), and once he has developed his own style, he should deliberately work to dispense with explicit allegory(無寄託). Consequently, discussing the technique of learning to write tz'u, he suggests that the novice should take Wang Yi-Sun(王沂孫) of Southern Sung as a writer to start with, who although wrote allegorically the significance implied in his tz'u could be easily understood, and finally should reach Chou Pang-yen(周邦彦) of Northern Sung who expressed the stage of the vagueness of allegory, via Wu Wen-ying(吳文英) and Hsin Ch'i-chi(辛棄疾) who showed the method of simple allegory. Because among these four tz'u writers, only Chou Pang-yen was of Northern Sung, we regard Chou Chi's theory of study tz'u as "From South to North"(由南追北論). Chou Chi presents two styles(風格) : "the clearness(淸?) and "the implication"(含蓄). The former is reflected by the use of allegory, the latter by the vagueness of allegory. The clearness is one of the characteristics of tz'u of Southern Sung, in which the allegory is sparse, while the implication is one of the characteristics of tz'u of Northern Sung, in which the allegory is thorough. If the allegory is thorough, it reveals different feelings and tastes. In other words, the deeper the meaning expressed allegorically is, the more different images it reflected to readers. This is the very stage of the vagueness of allegory. Chou Chi calls this style "the implication" and takes it for ideal style of tz'u. There are T'an Hsien(譚獻, 1832~1901), Ch'en T'ingch'ao(陳廷?, 1833~1892), K'uang chou-i(況周이, 1859~1926) and Wang Kuo-wei(王國維, 1877~1927) who are influenced by Chou Chi's theory of allegory. T'an Hsien, criticizing tz'u according to whether the allegory deep or not, enhanced Chou Chi's theory. Ch'en T'ing-ch'ao, who suggested the style called "Ch'en Yu(沈鬱)", elaborated the stage of vagueness of allegory, and set emphasis on the merit of the implication. K'uang Chou-I formed a base of the theory of writing called "Hsing-ling(性靈)" which emphasizes the fact that the feelings of one's personal affairs are the objects of allegory, and they are expressed unconsciously being affected by Hsing-ling. Wang Kuo-wei's theory of world(境界說) is also developed from Chou Chi's theory. In this regard, we can see that Chou Chi's theory of allegory greatly influenced on later theorists on tz'u. Especially, it should be noted that theory of allegory, originally a theory of criticism on tz'u, developed into that of writing and of appreciation.

揚州八怪, 羅聘의 生涯와 繪畵 硏究

李鍾振 동국대학교 교육대학원 1984 교육논총 Vol.14 No.-